| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,751 | A→G | 20.4% | E611E (GAA→GAG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,751 | 0 | A | G | 20.4% | 48.1 / 7.3 | 34 | E611E (GAA→GAG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base A (7/20); new base G (5/2); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAT > NC_000913/1708603‑1708894 | caaTCTCCCGCGCCAAACCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAc < 1:1559656/148‑1 (MQ=255) tATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAcc < 1:1728567/150‑1 (MQ=255) tCGACCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCag < 2:1947099/150‑1 (MQ=255) cccGCGCCCAACCCCGCAACCAGGAACACCAACAGGCAAATCCGGAACCAGAACCCCAGGCCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaag < 2:596522/150‑1 (MQ=14) gcgactaaGCGCGCAATCAGGAACATCATCAGGATAATCAGCAACCAGCACAACAGGTCGATCCTCGTAAACCTGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAc < 1:1059263/144‑1 (MQ=14) ctccAACGCGCGCAAGCTGGAACAGCATCAGGCTAATGAGGAACCATATCAAGATGTCGATCCGCTAAAAGCCTCCGTCGAAGCAGCTATAGCACTTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACa < 1:676839/148‑1 (MQ=14) cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACa < 1:1028296/150‑1 (MQ=255) cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACa < 1:1806748/150‑1 (MQ=255) ccaactcgcgcAAGCTGGAACACCAACAGGATAATCAGGAACCAGAACCACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg < 1:161097/144‑1 (MQ=14) atgCTGTAAGAGCTACAGGCTAATCAGGAAGCAGCACCAGAGGTGGATCCTCGCAAAGCCGCCGTGGAAGCAGCTATAGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCAATCCgcgc < 2:1774532/148‑1 (MQ=14) aagatcAACAGGCTAATTCGGAACCAGAACAAGAGGTCGATCCGCGCAAATCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccg < 2:1950816/145‑1 (MQ=255) aagatcAACAGGATACTCCCGAACCAGAACACGAGGTCGATCCTCGTAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccg < 2:503254/145‑1 (MQ=14) aaCACCAACAGGATAATTAGGAACCAGCACAAGTGGTCGATCCTCGCAAATCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccg < 1:1166187/150‑1 (MQ=14) tGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTAATGCGGAGCCagaaga > 2:2072134/1‑111 (MQ=255) acaacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTAGCAGCCGCTATTGCCCGAGCCaa > 1:1969601/1‑150 (MQ=255) caacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCGAAGCCGCGCGCGAAGACTCTTTTGCGCGTggaaag > 2:1232782/1‑145 (MQ=11) agaGGTGGATCCTCGCAAAGCGGCAGTCGATGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAGAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGc < 1:701101/148‑1 (MQ=14) caGGTTGAGCCTCGCAAATCCGCAGTAGAAGCCGCTATTGCCCTTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCa < 1:1026686/150‑1 (MQ=14) ccgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCaga > 1:2065235/1‑73 (MQ=21) cgccgcCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGCACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACa < 1:708174/150‑1 (MQ=255) aGCCGCCATTGCCCCGGCCCAACCGCGCAAGCTGGAACACCAACAGGCAAATGCGGAACCAGCACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc < 1:20133/150‑1 (MQ=11) ccAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAGGCCGCCGTCGACGCCGCTTTTGACCGTGCCCAAGCACGCAAGCTGTCACAGCAACAGGGTAATGCAGAGCAAGAACAGCAgg > 1:1641112/1‑150 (MQ=14) cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCgcgc < 1:1372695/64‑1 (MQ=255) cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCgcgc > 2:1372695/1‑64 (MQ=25) cgcgcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 2:2087703/79‑1 (MQ=11) cgcgcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCGGc > 1:2087703/1‑79 (MQ=11) cAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 2:2012936/1‑150 (MQ=255) aGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGccgccg < 1:2012936/150‑1 (MQ=255) aaCGGGCTAATGCGTAAGCACAAGAACTTTTCTATCTTAGCATAGCCGACGTTGAAGCCTCTTTTGCGCGCGCCATTGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCg < 2:1924067/150‑1 (MQ=2) tAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGcc < 2:1961450/67‑1 (MQ=25) tAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGcc > 1:1961450/1‑67 (MQ=25) aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCCAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTa > 1:751097/1‑150 (MQ=255) tGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGACGAGCCAGCACACCAGGTCCATCCCCGCAACGCAGCCGTCGCAGACGCTAt > 1:1651674/1‑150 (MQ=14) tGCGGAACCAGAAGAAAAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGTGCCAACGCACCCAAGCTGGAACAGCAGCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAt > 1:1990412/1‑150 (MQ=14) | CTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAT > NC_000913/1708603‑1708894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |