Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,366 T→A 9.1% intergenic (+23/+24) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,3660TA 9.1% 267.2 / 6.5 105intergenic (+23/+24)slyB/slyAouter membrane lipoprotein/global transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (62/32);  new base A (3/7);  total (65/40)
Fisher's exact test for biased strand distribution p-value = 3.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTC  >  NC_000913/1720217‑1720515
                                                                                                                                                      |                                                                                                                                                      
   aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTCCTGGCCCGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                    >  1:1219165/1‑150 (MQ=255)
   aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                    >  2:459203/1‑150 (MQ=255)
      tCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACTCTCGTTTCTCTACGGGCCTACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                                 <  2:1730758/150‑1 (MQ=255)
        gTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACca                                                                                                                                               >  1:2055243/1‑150 (MQ=255)
        gTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACca                                                                                                                                               <  2:443506/150‑1 (MQ=255)
          acaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                             <  2:444643/148‑1 (MQ=255)
          aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACcaca                                                                                                                                             <  1:1365718/150‑1 (MQ=255)
          aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGAGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                             >  1:501474/1‑150 (MQ=255)
           aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                            <  2:600715/150‑1 (MQ=255)
             catgATGGTATTACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGCCCTTTTCTCCGCGCTAAATAATTTTGCGTGTGGTCCGGTTCCTGGCCCCCCGc                                                                                                                                          >  1:454869/4‑150 (MQ=255)
               cGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAACACACGccc                                                                                                                                        >  1:439916/1‑150 (MQ=255)
               cGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCACCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGccc                                                                                                                                        >  1:1162500/1‑150 (MQ=255)
                    gTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTc                                                                                                                                   >  1:190668/1‑150 (MQ=255)
                      aaTACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCAAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCAt                                                                                                                                 >  2:1581476/1‑150 (MQ=255)
                          ccATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCa                                                                                                                             <  2:24496/150‑1 (MQ=255)
                           catcatGGTGGTACAGAAACAAGGCAACAATCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                            <  1:1288749/150‑1 (MQ=255)
                            atcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAcc                                                                                                                           >  1:862781/1‑150 (MQ=255)
                            atcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCATCATTTCAcc                                                                                                                           >  1:617746/1‑150 (MQ=255)
                             tcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTCGCCAGCAAAGGCAGTCACGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCACGTTACAGACCACACGCCCCCTTCATTTCAccc                                                                                                                          >  1:164501/1‑150 (MQ=255)
                              catGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCt                                                                                                                         >  1:2195458/1‑150 (MQ=255)
                                  gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTGGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTgg                                                                                                                     <  1:1560848/150‑1 (MQ=255)
                                      tACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTg                                                                                                                 >  1:2077406/1‑150 (MQ=255)
                                       aCAGAAACACGCCAACACTGGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCTGCATTGGCAGTCCGGTGACCGTTTCTCCGCGCTAAATACGTTTGGGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGt                                                                                                                <  2:2055844/150‑1 (MQ=255)
                                        cataaaCAAGGCAACACTCGTTTCTCTCCGGGCAAACGTGTCTTACTGGCCCGCAATGGCAGTCAGGTCACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTTACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                               <  1:5827/147‑1 (MQ=255)
                                        cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGGTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                               <  2:313649/150‑1 (MQ=255)
                                        cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACATGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCTCTAAATAAGTTTGCGTTTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                               >  2:425798/1‑150 (MQ=255)
                                        aacaaaCACGCCAATACTCTTTTGTCTCCGGGCCAAGGTGGCTTACTGGCAATATTTGGCTGTGTGGTGAGTGTTTCTCCGCTAAAAATATGTTGGGGTGTGGTTTGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                               <  2:1767666/147‑1 (MQ=255)
                                         aGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                              >  2:5030/1‑150 (MQ=255)
                                          gAAACAAGGCTACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGTCAGTCAGGTTACCGTTTCTCCGCGCAAAATAAGTTTGCGTGGGGTCAGTTTACTTACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                             <  1:1365683/150‑1 (MQ=255)
                                           aaaCAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACt                                                                                                            >  2:1969729/1‑150 (MQ=255)
                                                cggCACCTCTCGCTTCCCTCCGGGCCCTCGTGGCGTACTGGCCCGCAAGGGCAGTCAGGGGACCGTTTCGCCGCGCTAAATTAGGTGGGGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATg                                                                                                       <  1:331534/149‑1 (MQ=255)
                                                cggCAACACTGGTTTCTCTCCGGGCCTCCGTTGCGTAGTGGCCAGCATTGGCAGTCAGGTGACCTTTTCTCCGCGCTAAATATGTTTGGGTGTGGGCAGGTAACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATg                                                                                                       <  2:180157/149‑1 (MQ=255)
                                                aGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATg                                                                                                       <  1:302324/150‑1 (MQ=255)
                                                  gCAACACTCGTTTGTCTCCGGGCCAACGTGTGGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGat                                                                                                     <  1:58116/150‑1 (MQ=255)
                                                  gCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACACTTTGGCCTGTAACTCAATGat                                                                                                     >  2:693906/1‑150 (MQ=255)
                                                     acacTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGAtatt                                                                                                  <  2:247192/150‑1 (MQ=255)
                                                           gcttCTCTCCGGCCCACCGGGGCGTCCTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATACGTTTGCGTGCGGTCCGCTCCCTGACCACACGCCCCCATCATTTCACCCTTTGGCCTGTAACTCAATGATATAATGCTc                                                                                            <  2:349698/148‑1 (MQ=255)
                                                           gTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTc                                                                                            >  1:1323676/1‑150 (MQ=255)
                                                                  tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCACCCGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                     >  1:49861/1‑150 (MQ=255)
                                                                    cGGGCCAACGTGTCGTACTGGCCTGCATTGTCAGTCAGGTGACCTTTTCTCCCCGAAAAATACGTTTGCGTTTGGTCTGGTTAGTGACCACACGCCCCCTTCATATCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                   <  1:1262746/150‑1 (MQ=255)
                                                                    cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCGGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                   >  1:620684/1‑150 (MQ=255)
                                                                    cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACCCGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                   >  2:2191634/1‑150 (MQ=255)
                                                                     gggCCAACGTGTCGAACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCCCTAAATAAGTATCCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATATCACCCAATCGCCTCTAACTCAATGATATTATGCTCAACATTTGCg                                                                                  >  2:808245/1‑150 (MQ=255)
                                                                      ggCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGa                                                                                 >  2:813893/1‑150 (MQ=255)
                                                                         cAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATgg                                                                              >  2:941130/1‑149 (MQ=255)
                                                                         cAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGa                                                                              >  1:1598973/1‑150 (MQ=255)
                                                                            ggtgtCGTACTGGCCTGCATTGGAAGTCAGGTGACCGTTTCTCCGCGATAAATATGTTTGCGTGTGGTCAGGTTATTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCg                                                                           <  2:870621/149‑1 (MQ=255)
                                                                            cggttCGTACTGGCCAGCATGGCCCGTCGGTGTCCCGTTTCTCCGCGCTAAATAAGTTTGCGTGGGTACAGTTTACAGCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCg                                                                           <  2:417934/146‑1 (MQ=255)
                                                                              tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACCCGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                         >  1:1465944/1‑150 (MQ=255)
                                                                               gtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTaa                                                                        >  1:1447411/1‑150 (MQ=255)
                                                                                 cGTACTGTCCAGAAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATc                                                                      <  2:28443/150‑1 (MQ=255)
                                                                                   tACTGGCCAGCAATGGCATTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTTGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAg                                                                    <  2:1269035/150‑1 (MQ=255)
                                                                                   tACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCACACGCCCCATTCATTTCAGCCGTTGGCCTGTACCTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAg                                                                    >  1:138573/1‑150 (MQ=255)
                                                                                       ggCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGc                                                                >  2:1534587/1‑150 (MQ=255)
                                                                                        gCCAGCAATGGCCGTCAGGTGACCGTTTCGCCGCGCTAAATACGTTTGCGTGTGGTCAGGTTACTTACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCt                                                               <  1:1166517/150‑1 (MQ=255)
                                                                                        gCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCt                                                               >  1:693647/1‑150 (MQ=255)
                                                                                         ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                              >  2:2145732/1‑150 (MQ=255)
                                                                                         ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                              >  1:414402/1‑150 (MQ=255)
                                                                                            gCAATGGCAGTCAGTGTCCCGTTTCTCCGCCCAAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAg                                                           <  1:379551/150‑1 (MQ=255)
                                                                                            gCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCATTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAg                                                           >  1:1619785/1‑150 (MQ=255)
                                                                                              aaTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGtt                                                         >  2:902929/1‑150 (MQ=255)
                                                                                               aTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTc                                                        >  1:479329/1‑150 (MQ=255)
                                                                                               aTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTc                                                        >  1:265299/1‑150 (MQ=255)
                                                                                                   cAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACATGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCctct                                                    >  2:882078/1‑150 (MQ=255)
                                                                                                   cAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCctct                                                    >  2:1214402/1‑150 (MQ=255)
                                                                                                     gTCTGGTGACCGTTTCTCCGCTATAAAAATTTTGGCGGGTGGTCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGc                                                  <  1:2084943/150‑1 (MQ=255)
                                                                                                     gTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGc                                                  <  1:1520470/150‑1 (MQ=255)
                                                                                                       cAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTTGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCAATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                                >  2:1976780/1‑150 (MQ=255)
                                                                                                         gttGACCGTTTGGCGGCGCTAAATAAGTTTGGGTGGGGTCAGGTAACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGCAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgag                                              <  2:148393/148‑1 (MQ=255)
                                                                                                         ggTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgag                                              <  2:1162500/150‑1 (MQ=255)
                                                                                                                cGTTTCCCCGCCCTAAATAAGTTTGCGGGGGGACTGGTAACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCa                                        <  2:190668/150‑1 (MQ=255)
                                                                                                                 gTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                       >  1:137698/1‑150 (MQ=255)
                                                                                                                 gTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                       >  2:790542/1‑150 (MQ=255)
                                                                                                                 gTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCGTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                       >  2:91407/1‑150 (MQ=255)
                                                                                                                    tctcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAACACACGCACCATTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTAAAGTTTTGCTATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTa                                    >  2:288089/1‑150 (MQ=255)
                                                                                                                     ctcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCGCCGTCATTTCCCCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTaa                                   >  2:1176811/1‑150 (MQ=255)
                                                                                                                       ccGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCCCCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAata                                 >  1:610103/1‑150 (MQ=255)
                                                                                                                        cgcgCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAGGTAatat                                >  1:1374494/1‑150 (MQ=255)
                                                                                                                        cgcgCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTAACCCTTTGGCCTGTAACTAAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAatat                                >  1:2206749/1‑150 (MQ=255)
                                                                                                                         gctcTAAATAAGTGGGGTGGGGTTAGGTTACCTCACCACACGCCCCCTTCATTTCACCCTTTGGCATGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATAtt                               <  1:2152718/147‑1 (MQ=255)
                                                                                                                         gcgcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTGCATTTCACGCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTTATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTTATAtt                               >  1:319655/1‑150 (MQ=255)
                                                                                                                             taataatgTTTGCGGGGGGGCTGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcg                           <  2:1741984/143‑1 (MQ=255)
                                                                                                                             aaaaTAAGTTTTCGTGTGGTCAGTTTAATGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcg                           <  1:1432301/149‑1 (MQ=255)
                                                                                                                              aaaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTATCTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCAATGTAATATTTCcgc                          >  1:347815/1‑150 (MQ=255)
                                                                                                                                 tAAGTTTGCGTGTGGTCAGGTTACTGACCACAGGCCCCCTTCATTTCACCCTTTGGCCTGTAAGTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTTATCAGTTGCTCCAGTTCCTCAGCGGAGATGCCATGTAATATTTCcgcgcg                       >  1:1097637/1‑150 (MQ=255)
                                                                                                                                    gTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATGTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCTCGGGt                    >  2:1590475/1‑150 (MQ=255)
                                                                                                                                         cGTGTGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt               >  2:1272562/1‑150 (MQ=255)
                                                                                                                                         cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt               >  2:54704/1‑150 (MQ=255)
                                                                                                                                         cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt               >  2:1246721/1‑150 (MQ=255)
                                                                                                                                         cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATAGTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGAAATATTTCCGCGAGGGTTTTGt               >  2:1328940/1‑150 (MQ=255)
                                                                                                                                          gtgtGGTCAGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGTGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt              <  2:2055243/150‑1 (MQ=255)
                                                                                                                                          gtgtGGTCAGTTACCTCCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt              <  1:488534/150‑1 (MQ=255)
                                                                                                                                          gtgtGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGGTATCAGTTGCTCCAGTTCCTCTGCGGAGCTGCCATGTAATATTTCCGCGCGGGTTTTGtt              >  2:1333903/1‑150 (MQ=255)
                                                                                                                                           tgtgGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTta             >  2:1849155/1‑150 (MQ=255)
                                                                                                                                            ggggTCTGGTATCTTACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTATCTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaa            <  1:221532/148‑1 (MQ=255)
                                                                                                                                              ggTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATCTAATATTTCCGCGCGGGTTTTGTtaata          >  2:30622/1‑150 (MQ=255)
                                                                                                                                               gTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGCTGCCATGTAATATTTCCGCGCGGGTTTTGTtaataa         >  1:18162/1‑150 (MQ=255)
                                                                                                                                                 cAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACa       <  1:1508697/150‑1 (MQ=255)
                                                                                                                                                      tACTTACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTc  <  2:862781/150‑1 (MQ=255)
                                                                                                                                                      tACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTc  <  1:1295508/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTC  >  NC_000913/1720217‑1720515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: