Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,367 A→C 5.5% intergenic (+24/+23) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,3670AC 5.5% 276.6 / 4.1 107intergenic (+24/+23)slyB/slyAouter membrane lipoprotein/global transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (61/37);  new base C (4/3);  total (65/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 108 total aligned reads displayed.

AATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTCC  >  NC_000913/1720220‑1720516
                                                                                                                                                    |                                                                                                                                                      
aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTCCTGGCCCGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                     >  1:1219165/1‑150 (MQ=255)
aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                     >  2:459203/1‑150 (MQ=255)
     gTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACca                                                                                                                                                <  2:443506/150‑1 (MQ=255)
     gTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACca                                                                                                                                                >  1:2055243/1‑150 (MQ=255)
       acaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                              <  2:444643/148‑1 (MQ=255)
       aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACcaca                                                                                                                                              <  1:1365718/150‑1 (MQ=255)
       aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGAGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                              >  1:501474/1‑150 (MQ=255)
        aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                             <  2:600715/150‑1 (MQ=255)
          catgATGGTATTACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGCCCTTTTCTCCGCGCTAAATAATTTTGCGTGTGGTCCGGTTCCTGGCCCCCCGc                                                                                                                                           >  1:454869/4‑150 (MQ=255)
            cGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCACCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGccc                                                                                                                                         >  1:1162500/1‑150 (MQ=255)
                 gTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTc                                                                                                                                    >  1:190668/1‑150 (MQ=255)
                   aaTACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCAAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCAt                                                                                                                                  >  2:1581476/1‑150 (MQ=255)
                       ccATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCa                                                                                                                              <  2:24496/150‑1 (MQ=255)
                        catcatGGTGGTACAGAAACAAGGCAACAATCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                             <  1:1288749/150‑1 (MQ=255)
                         atcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCATCATTTCAcc                                                                                                                            >  1:617746/1‑150 (MQ=255)
                          tcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTCGCCAGCAAAGGCAGTCACGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCACGTTACAGACCACACGCCCCCTTCATTTCAccc                                                                                                                           >  1:164501/1‑150 (MQ=255)
                           catGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCt                                                                                                                          >  1:2195458/1‑150 (MQ=255)
                               gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTGGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTgg                                                                                                                      <  1:1560848/150‑1 (MQ=255)
                                    aCAGAAACACGCCAACACTGGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCTGCATTGGCAGTCCGGTGACCGTTTCTCCGCGCTAAATACGTTTGGGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGt                                                                                                                 <  2:2055844/150‑1 (MQ=255)
                                     cataaaCAAGGCAACACTCGTTTCTCTCCGGGCAAACGTGTCTTACTGGCCCGCAATGGCAGTCAGGTCACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTTACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                                <  1:5827/147‑1 (MQ=255)
                                     cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGGTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                                <  2:313649/150‑1 (MQ=255)
                                     cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACATGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCTCTAAATAAGTTTGCGTTTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                                >  2:425798/1‑150 (MQ=255)
                                     aacaaaCACGCCAATACTCTTTTGTCTCCGGGCCAAGGTGGCTTACTGGCAATATTTGGCTGTGTGGTGAGTGTTTCTCCGCTAAAAATATGTTGGGGTGTGGTTTGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                                <  2:1767666/147‑1 (MQ=255)
                                      aGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                               >  2:5030/1‑150 (MQ=255)
                                       gAAACAAGGCTACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGTCAGTCAGGTTACCGTTTCTCCGCGCAAAATAAGTTTGCGTGGGGTCAGTTTACTTACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                              <  1:1365683/150‑1 (MQ=255)
                                        aaaCAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACt                                                                                                             >  2:1969729/1‑150 (MQ=255)
                                             cggCACCTCTCGCTTCCCTCCGGGCCCTCGTGGCGTACTGGCCCGCAAGGGCAGTCAGGGGACCGTTTCGCCGCGCTAAATTAGGTGGGGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATg                                                                                                        <  1:331534/149‑1 (MQ=255)
                                             cggCAACACTGGTTTCTCTCCGGGCCTCCGTTGCGTAGTGGCCAGCATTGGCAGTCAGGTGACCTTTTCTCCGCGCTAAATATGTTTGGGTGTGGGCAGGTAACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATg                                                                                                        <  2:180157/149‑1 (MQ=255)
                                             aGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATg                                                                                                        <  1:302324/150‑1 (MQ=255)
                                               gCAACACTCGTTTGTCTCCGGGCCAACGTGTGGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGat                                                                                                      <  1:58116/150‑1 (MQ=255)
                                               gCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACACTTTGGCCTGTAACTCAATGat                                                                                                      >  2:693906/1‑150 (MQ=255)
                                                  acacTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGAtatt                                                                                                   <  2:247192/150‑1 (MQ=255)
                                                        gcttCTCTCCGGCCCACCGGGGCGTCCTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATACGTTTGCGTGCGGTCCGCTCCCTGACCACACGCCCCCATCATTTCACCCTTTGGCCTGTAACTCAATGATATAATGCTc                                                                                             <  2:349698/148‑1 (MQ=255)
                                                        gTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTc                                                                                             >  1:1323676/1‑150 (MQ=255)
                                                               tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCACCCGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                      >  1:49861/1‑150 (MQ=255)
                                                                ccGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTATCCCCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCATTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTg                                                                                     >  1:20707/1‑150 (MQ=255)
                                                                 cGGGCCAACGTGTCGTACTGGCCTGCATTGTCAGTCAGGTGACCTTTTCTCCCCGAAAAATACGTTTGCGTTTGGTCTGGTTAGTGACCACACGCCCCCTTCATATCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                    <  1:1262746/150‑1 (MQ=255)
                                                                 cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCGGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                    >  1:620684/1‑150 (MQ=255)
                                                                 cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACCCGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                    >  2:2191634/1‑150 (MQ=255)
                                                                  gggCCAACGTGTCGAACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCCCTAAATAAGTATCCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATATCACCCAATCGCCTCTAACTCAATGATATTATGCTCAACATTTGCg                                                                                   >  2:808245/1‑150 (MQ=255)
                                                                   ggCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGa                                                                                  >  2:813893/1‑150 (MQ=255)
                                                                      cAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATgg                                                                               >  2:941130/1‑149 (MQ=255)
                                                                      cAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGa                                                                               >  1:1598973/1‑150 (MQ=255)
                                                                         ggtgtCGTACTGGCCTGCATTGGAAGTCAGGTGACCGTTTCTCCGCGATAAATATGTTTGCGTGTGGTCAGGTTATTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCg                                                                            <  2:870621/149‑1 (MQ=255)
                                                                         cggttCGTACTGGCCAGCATGGCCCGTCGGTGTCCCGTTTCTCCGCGCTAAATAAGTTTGCGTGGGTACAGTTTACAGCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCg                                                                            <  2:417934/146‑1 (MQ=255)
                                                                           tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACCCGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                          >  1:1465944/1‑150 (MQ=255)
                                                                            gtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTaa                                                                         >  1:1447411/1‑150 (MQ=255)
                                                                              cGTACTGTCCAGAAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATc                                                                       <  2:28443/150‑1 (MQ=255)
                                                                                tACTGGCCAGCAATGGCATTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTTGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAg                                                                     <  2:1269035/150‑1 (MQ=255)
                                                                                tACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCACACGCCCCATTCATTTCAGCCGTTGGCCTGTACCTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAg                                                                     >  1:138573/1‑150 (MQ=255)
                                                                                    ggCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGc                                                                 >  2:1534587/1‑150 (MQ=255)
                                                                                     gCCAGCAATGGCCGTCAGGTGACCGTTTCGCCGCGCTAAATACGTTTGCGTGTGGTCAGGTTACTTACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCt                                                                <  1:1166517/150‑1 (MQ=255)
                                                                                     gCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCt                                                                >  1:693647/1‑150 (MQ=255)
                                                                                      ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                               >  1:414402/1‑150 (MQ=255)
                                                                                      ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                               >  2:2145732/1‑150 (MQ=255)
                                                                                         gCAATGGCAGTCAGTGTCCCGTTTCTCCGCCCAAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAg                                                            <  1:379551/150‑1 (MQ=255)
                                                                                         gCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCATTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAg                                                            >  1:1619785/1‑150 (MQ=255)
                                                                                           aaTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGtt                                                          >  2:902929/1‑150 (MQ=255)
                                                                                            aTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTc                                                         >  1:265299/1‑150 (MQ=255)
                                                                                            aTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTc                                                         >  1:479329/1‑150 (MQ=255)
                                                                                                cAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACATGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCctct                                                     >  2:882078/1‑150 (MQ=255)
                                                                                                cAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCctct                                                     >  2:1214402/1‑150 (MQ=255)
                                                                                                  gTCTGGTGACCGTTTCTCCGCTATAAAAATTTTGGCGGGTGGTCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGc                                                   <  1:2084943/150‑1 (MQ=255)
                                                                                                  gTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGc                                                   <  1:1520470/150‑1 (MQ=255)
                                                                                                    cAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTTGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCAATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                                 >  2:1976780/1‑150 (MQ=255)
                                                                                                      gttGACCGTTTGGCGGCGCTAAATAAGTTTGGGTGGGGTCAGGTAACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGCAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgag                                               <  2:148393/148‑1 (MQ=255)
                                                                                                      ggTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgag                                               <  2:1162500/150‑1 (MQ=255)
                                                                                                             cGTTTCCCCGCCCTAAATAAGTTTGCGGGGGGACTGGTAACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCa                                         <  2:190668/150‑1 (MQ=255)
                                                                                                              gTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                        >  1:137698/1‑150 (MQ=255)
                                                                                                              gTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                        >  2:790542/1‑150 (MQ=255)
                                                                                                              gTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCGTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                        >  2:91407/1‑150 (MQ=255)
                                                                                                                 tctcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAACACACGCACCATTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTAAAGTTTTGCTATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTa                                     >  2:288089/1‑150 (MQ=255)
                                                                                                                  ctcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCGCCGTCATTTCCCCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTaa                                    >  2:1176811/1‑150 (MQ=255)
                                                                                                                    ccGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCCCCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAata                                  >  1:610103/1‑150 (MQ=255)
                                                                                                                     cgcgCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAGGTAatat                                 >  1:1374494/1‑150 (MQ=255)
                                                                                                                     cgcgCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTAACCCTTTGGCCTGTAACTAAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAatat                                 >  1:2206749/1‑150 (MQ=255)
                                                                                                                      gctcTAAATAAGTGGGGTGGGGTTAGGTTACCTCACCACACGCCCCCTTCATTTCACCCTTTGGCATGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATAtt                                <  1:2152718/147‑1 (MQ=255)
                                                                                                                      gcgcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATAtt                                >  1:4292/1‑150 (MQ=255)
                                                                                                                      gcgcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTGCATTTCACGCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTTATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTTATAtt                                >  1:319655/1‑150 (MQ=255)
                                                                                                                          taataatgTTTGCGGGGGGGCTGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcg                            <  2:1741984/143‑1 (MQ=255)
                                                                                                                           aaaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTATCTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCAATGTAATATTTCcgc                           >  1:347815/1‑150 (MQ=255)
                                                                                                                            aaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCAAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcg                          >  1:862212/1‑150 (MQ=255)
                                                                                                                              tAAGTTTGCGTGTGGTCAGGTTACTGACCACAGGCCCCCTTCATTTCACCCTTTGGCCTGTAAGTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTTATCAGTTGCTCCAGTTCCTCAGCGGAGATGCCATGTAATATTTCcgcgcg                        >  1:1097637/1‑150 (MQ=255)
                                                                                                                                 gTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATGTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCTCGGGt                     >  2:1590475/1‑150 (MQ=255)
                                                                                                                                      cGTGTGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt                >  2:1272562/1‑150 (MQ=255)
                                                                                                                                      cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt                >  2:54704/1‑150 (MQ=255)
                                                                                                                                      cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt                >  2:1246721/1‑150 (MQ=255)
                                                                                                                                      cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATAGTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGAAATATTTCCGCGAGGGTTTTGt                >  2:1328940/1‑150 (MQ=255)
                                                                                                                                       gtgtGGTCAGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGTGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt               <  2:2055243/150‑1 (MQ=255)
                                                                                                                                       gtgtGGTCAGTTACCTCCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt               <  1:488534/150‑1 (MQ=255)
                                                                                                                                       gtgtGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGGTATCAGTTGCTCCAGTTCCTCTGCGGAGCTGCCATGTAATATTTCCGCGCGGGTTTTGtt               >  2:1333903/1‑150 (MQ=255)
                                                                                                                                        tgtgGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTta              >  2:1849155/1‑150 (MQ=255)
                                                                                                                                         ggggTCTGGTATCTTACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTATCTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaa             <  1:221532/148‑1 (MQ=255)
                                                                                                                                           ggTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATCTAATATTTCCGCGCGGGTTTTGTtaata           >  2:30622/1‑150 (MQ=255)
                                                                                                                                            gTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGCTGCCATGTAATATTTCCGCGCGGGTTTTGTtaataa          >  1:18162/1‑150 (MQ=255)
                                                                                                                                              cAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACa        <  1:1508697/150‑1 (MQ=255)
                                                                                                                                              aatgTTTCTTACCACACGCCCCCTTCATTTCACCCTTTGGGCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGGGGAGATGCCATGTAATTTTTCCGCGCGGGTTTTGTTAATAACa        <  2:164501/147‑1 (MQ=255)
                                                                                                                                                   tACTTACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTc   <  2:862781/150‑1 (MQ=255)
                                                                                                                                                   tACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTc   <  1:1295508/150‑1 (MQ=255)
                                                                                                                                                    aCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTcc  <  2:2195458/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
AATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTCC  >  NC_000913/1720220‑1720516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: