BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,747,149	G→T	9.9%	V7L (GTG→TTG)	ydhS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,747,149	0	G	T	9.9%	288.3 / 9.4	104	V7L (GTG→TTG)	ydhS	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (48/44); new base T (3/8); total (52/52)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

GCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAAT  >  NC_000913/1747000‑1747298

gCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATTCGTACAGGTAAGTAACAATGAAAAAAATTGCTATtg                                                                                                                                                       >  1:575695/1‑150 (MQ=255)

 ccTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATtgt                                                                                                                                                      <  1:1287325/150‑1 (MQ=255)

    gACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTggg                                                                                                                                                   >  2:2021996/1‑150 (MQ=255)

       aGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGc                                                                                                                                                >  1:2083858/1‑150 (MQ=255)

        gAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCGGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGcc                                                                                                                                               >  1:1784288/1‑150 (MQ=255)

          aTTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCgg                                                                                                                                             >  1:1400810/1‑150 (MQ=255)

          aTTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCgg                                                                                                                                             <  1:897935/150‑1 (MQ=255)

           tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCggg                                                                                                                                            <  2:824634/150‑1 (MQ=255)

               aTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTa                                                                                                                                        <  1:1296706/150‑1 (MQ=255)

               aTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTa                                                                                                                                        >  1:218730/1‑150 (MQ=255)

                 cAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCTAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACg                                                                                                                                      >  2:2018267/1‑150 (MQ=255)

                     ggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGa                                                                                                                                  <  2:575840/150‑1 (MQ=255)

                       cGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGATATTTTGCGTGCCGGGCCTACGGGGATc                                                                                                                                >  2:1041986/1‑150 (MQ=255)

                        ggTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATTCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCt                                                                                                                               <  2:575695/150‑1 (MQ=255)

                         gctAGCGCCCTTTTCATCCCAGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATTACACTAAAGTCGAAAGAATGCGTACAGGTAAGTAACAATGAAATAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTa                                                                                                                              <  1:329889/148‑1 (MQ=255)

                           tAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAATAATGTGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTaca                                                                                                                            <  1:1184020/150‑1 (MQ=255)

                           tAGCGCCCTTTTCATCCCTGTATGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTaca                                                                                                                            <  2:1424584/150‑1 (MQ=255)

                             gctcccTTTTGATCCCTGTCAGAAATTTCTCAAATTCTAAAAATCTAAACCAAACTTATCTGATAACACTAAATTCGCAAGAATGCGTACTGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACAcc                                                                                                                          <  1:2073113/147‑1 (MQ=255)

                             gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACATGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACAcc                                                                                                                          <  1:383621/150‑1 (MQ=255)

                             gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACACTGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACAcc                                                                                                                          <  1:1301644/150‑1 (MQ=255)

                             gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACAcc                                                                                                                          <  1:2182991/150‑1 (MQ=255)

                             gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTAAAGGTAAGTAACAATGAAAAAAATTGCTATTTTGGGTCCCGGCCCTACGGGGATCTACAcc                                                                                                                          >  2:944282/1‑150 (MQ=255)

                               gcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTAAAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCtt                                                                                                                        >  2:114031/1‑150 (MQ=255)

                                  cTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTAtt                                                                                                                     <  1:1272671/150‑1 (MQ=255)

                                      tCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTGAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCg                                                                                                                 <  1:1815263/150‑1 (MQ=255)

                                      tCATACCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTCGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCg                                                                                                                 <  1:2008979/150‑1 (MQ=255)

                                       cATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTAATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGAAACAATGAAAAAAATTGCTATATTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGc                                                                                                                >  2:1934983/1‑150 (MQ=255)

                                       cATCACTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGc                                                                                                                >  2:1144945/1‑150 (MQ=255)

                                            cTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTa                                                                                                           <  1:1060636/150‑1 (MQ=255)

                                             tGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTAc                                                                                                          <  1:644580/150‑1 (MQ=255)

                                              gTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACa                                                                                                         >  1:480587/1‑150 (MQ=255)

                                                cTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGACCTACGGGGATCTACACCTTATTCTCGCTTCTACAGc                                                                                                       >  2:903335/1‑150 (MQ=255)

                                                 tGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGca                                                                                                      >  2:1551392/1‑150 (MQ=255)

                                                   aaaTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGcaac                                                                                                    <  1:518444/150‑1 (MQ=255)

                                                         ctcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                              >  1:460376/1‑150 (MQ=255)

                                                         ctcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATAGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTAAAGCAACAAACTc                                                                                              >  2:427586/1‑150 (MQ=255)

                                                          tcAAATTCTAAAAATCTCAACCAAACTTATCTGATCACACTAAATTGGAAAGAATGCGTCCAGGTAAGTACCAAAGAAAAAAATAGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTcc                                                                                             <  1:1427404/150‑1 (MQ=255)

                                                          tcAAATTCTAAAAATCTCAACCAAACTTATCTGATACCACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATAGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTcc                                                                                             >  1:1466716/1‑150 (MQ=255)

                                                          tcAAATTCTAAAAATCTCAACCAAACTTATCTGAGAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACtac                                                                                             >  2:1572629/1‑148 (MQ=255)

                                                           cAAATTCTAAAAATCTCAACCAACCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGTTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCa                                                                                            >  1:192522/1‑150 (MQ=255)

                                                           aaaaTTATAAAAATCTCAACCAAACTTATCTTATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTTCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCa                                                                                            <  1:475400/149‑1 (MQ=255)

                                                             aaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACt                                                                                          <  2:111032/150‑1 (MQ=255)

                                                              aTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtt                                                                                         >  1:1747346/1‑150 (MQ=255)

                                                              aTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtt                                                                                         >  1:211918/1‑150 (MQ=255)

                                                                  tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttcta                                                                                     >  1:1353098/1‑150 (MQ=255)

                                                                  tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTCTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCAACtttcta                                                                                     >  2:888419/1‑150 (MQ=255)

                                                                   aaaaaTCTCAACCAAACTTATATGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctat                                                                                    <  1:2063923/150‑1 (MQ=255)

                                                                    aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatt                                                                                   >  2:487033/1‑150 (MQ=255)

                                                                      aaTCTCAACCAAACTTATCTGATAACAATAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTTTTATTTTGGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttc                                                                                 <  2:1580970/150‑1 (MQ=255)

                                                                       aTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttct                                                                                >  2:547518/1‑150 (MQ=255)

                                                                          tcAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATc                                                                             >  2:1137505/1‑150 (MQ=255)

                                                                                aaaCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGATATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAg                                                                       >  1:759774/1‑150 (MQ=255)

                                                                                  aCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCa                                                                     <  1:1534755/150‑1 (MQ=255)

                                                                                   cTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATTTTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAg                                                                    <  2:1415453/150‑1 (MQ=255)

                                                                                     tATCTGATAACACTAAATTCGAAATAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGc                                                                  <  1:517809/150‑1 (MQ=255)

                                                                                     tATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGc                                                                  <  1:1605941/150‑1 (MQ=255)

                                                                                       tCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTTTTATTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTg                                                                <  2:1465409/150‑1 (MQ=255)

                                                                                         tGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                              >  1:2148379/1‑150 (MQ=255)

                                                                                          gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACTATGAAAAAAATTGCTATTGTGGGTGCGGGGCCTACGGGGATCTACACATTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGTGCAGGCTGACg                                                             <  1:600413/150‑1 (MQ=255)

                                                                                          gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTGCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACg                                                             <  1:164203/150‑1 (MQ=255)

                                                                                          gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGAAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACg                                                             >  2:2030555/1‑150 (MQ=255)

                                                                                           aTAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGTTGACGa                                                            <  1:806714/150‑1 (MQ=255)

                                                                                              agacTAAATTCGAAAGAATGCGTACATTTATGTAACAATGAAAAAAATTTCTATTGTGGGTGCCGGGCCTACGGGGTGCTACACCTTATTCTCTCTTCTACAGCAACAAACTCCACTTTCTTTTTCTATCTTCGAGCAGGCTGACGAGGc                                                         <  1:427586/148‑1 (MQ=255)

                                                                                              acacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATTTTTTTTTTTTGGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                         <  2:1595969/150‑1 (MQ=255)

                                                                                               cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                        >  2:2097657/1‑150 (MQ=255)

                                                                                               cacTAAATTCGAAAGAATGAGTACAGTTAAGTTACAATTATAAAAATTTCTATTGTGGGTGCCGGGCCTACGGGTATCTACACCTTATTCTCGCTTCTACAGCAACAAAGTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                        <  1:1934983/150‑1 (MQ=255)

                                                                                                 cTAAATTCGTAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTAATCTCGCTTCTACAGCACCAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGCGGCCgg                                                      <  1:295793/150‑1 (MQ=255)

                                                                                                 cTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTTTTTTTTTTGTTGCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCgg                                                      <  2:2103076/150‑1 (MQ=255)

                                                                                                  tAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCCACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTCCTATTTCTATCTTCGAGCAGGTTGACGAGGCCGgt                                                     >  2:1805543/1‑150 (MQ=255)

                                                                                                    aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgtgt                                                   >  1:88354/1‑150 (MQ=255)

                                                                                                       tCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCgg                                                >  2:192755/1‑150 (MQ=255)

                                                                                                       tCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCgg                                                <  2:1824682/150‑1 (MQ=255)

                                                                                                       tCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCgg                                                <  1:2018267/150‑1 (MQ=255)

                                                                                                        cGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCggg                                               >  2:955678/1‑150 (MQ=255)

                                                                                                         gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCggta                                              >  2:1157239/1‑148 (MQ=255)

                                                                                                           aaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATg                                            <  1:1879274/150‑1 (MQ=255)

                                                                                                             gAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGcc                                          <  1:152670/150‑1 (MQ=255)

                                                                                                             gAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGcc                                          <  1:2123227/150‑1 (MQ=255)

                                                                                                                tGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATa                                       <  1:944282/150‑1 (MQ=255)

                                                                                                                        ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatg                               >  1:881525/1‑150 (MQ=255)

                                                                                                                        ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatg                               >  1:154627/1‑150 (MQ=255)

                                                                                                                        ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatg                               >  1:947738/1‑150 (MQ=255)

                                                                                                                        ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatg                               <  1:1706985/150‑1 (MQ=255)

                                                                                                                        ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTATCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatg                               >  1:527411/1‑150 (MQ=255)

                                                                                                                          taagtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAg                             >  2:2101719/1‑150 (MQ=255)

                                                                                                                           aagtaaCAATGAAAATAATTGATATTGTGGTTTCCGGGCCTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCAAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAgg                            <  2:1784288/150‑1 (MQ=255)

                                                                                                                           aagtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAgg                            >  1:2096137/1‑150 (MQ=255)

                                                                                                                           aagtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAgg                            >  2:902588/1‑150 (MQ=255)

                                                                                                                                actaTGAAAAAAATTGCTATTGTGGGGGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCATGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAAc                       <  1:165960/147‑1 (MQ=255)

                                                                                                                                 cAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACt                      >  1:511557/1‑150 (MQ=255)

                                                                                                                                 cAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACt                      >  1:1875213/1‑150 (MQ=255)

                                                                                                                                   aTGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAATCTCa                    >  1:784792/1‑150 (MQ=255)

                                                                                                                                    tGAAAAAATTTGCTATTGTGGGTGCCGGGCCTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaa                   <  2:1070514/150‑1 (MQ=255)

                                                                                                                                      aaaaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCAACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaa                 >  1:2133269/1‑150 (MQ=255)

                                                                                                                                       aaaaaaTTGCTATTGGGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGCGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaaa                <  1:1801195/150‑1 (MQ=255)

                                                                                                                                          aaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatga             <  1:1144945/150‑1 (MQ=255)

                                                                                                                                               gCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTg        <  1:192755/150‑1 (MQ=255)

                                                                                                                                                  aTTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCa     >  1:1845101/1‑150 (MQ=255)

                                                                                                                                                     gtgGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAACCTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAat  >  1:1120997/1‑150 (MQ=255)

                                                                                                                                                     gtgGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGCCGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAat  >  1:2091864/1‑150 (MQ=255)

GCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAAT  >  NC_000913/1747000‑1747298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`