Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,822,071 G→A 6.6% I62I (ATC→ATT) ‡ osmE ← osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,822,0710GA 6.6% 428.5 / 11.9 136I62I (ATC→ATT) ‡osmEosmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (63/63);  new base A (3/6);  total (67/69)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

Only 100 of 141 total aligned reads displayed.

TTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCG  >  NC_000913/1821922‑1822219
                                                                                                                                                     |                                                                                                                                                    
ttCGCAGCCTTTGTATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATCACATGTCCGGTAACATCTAACGCGACAAATTAGGTTTCTGCTTAACCATCAGGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCTAGCATgg                                                                                                                                                      <  2:942756/150‑1 (MQ=255)
ttCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATgg                                                                                                                                                      <  2:1538684/150‑1 (MQ=255)
ttCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATgg                                                                                                                                                      <  2:1487276/150‑1 (MQ=255)
  cGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt                                                                                                                                                    <  1:2134342/150‑1 (MQ=255)
  cGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt                                                                                                                                                    <  2:258737/150‑1 (MQ=255)
         tgtgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc                                                                                                                                             <  1:1565911/150‑1 (MQ=255)
           tgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgc                                                                                                                                           <  2:821124/150‑3 (MQ=255)
           tgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgc                                                                                                                                           <  2:641759/150‑3 (MQ=255)
           tgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgc                                                                                                                                           <  2:7502/150‑3 (MQ=255)
           ggtctCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATTACATGTCCGGTATCATCTAACTCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCAc                                                                                                                                           <  2:1913542/146‑1 (MQ=255)
            ggATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTCACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgcg                                                                                                                                          <  1:1519503/150‑4 (MQ=255)
            ggATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACt                                                                                                                                          >  1:287224/1‑150 (MQ=255)
               tCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                                                                                                       <  2:482177/150‑1 (MQ=255)
                cAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAg                                                                                                                                      >  1:765109/1‑150 (MQ=255)
                    gtCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGa                                                                                                                                  >  2:646280/1‑150 (MQ=255)
                       gTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAgg                                                                                                                               >  2:1902340/1‑150 (MQ=255)
                        tATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGt                                                                                                                              <  1:98365/150‑1 (MQ=255)
                        tATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGt                                                                                                                              <  1:1902340/150‑1 (MQ=255)
                         aTTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                                                                                                             <  1:961215/150‑1 (MQ=255)
                          ttCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGttt                                                                                                                            >  1:2087237/1‑150 (MQ=255)
                           tCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTa                                                                                                                           <  1:1081019/150‑1 (MQ=255)
                            cAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTATAc                                                                                                                          >  1:340401/1‑150 (MQ=255)
                             aGCACAGGTCTGATATCCGGAGTTGATGACATGTCCGGTTTCATCTAACGCTACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAcc                                                                                                                         <  1:273511/150‑1 (MQ=255)
                                   ggTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACAATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAt                                                                                                                   <  2:1677413/150‑1 (MQ=255)
                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAtt                                                                                                                  >  2:1487018/1‑150 (MQ=255)
                                      cTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtg                                                                                                                >  2:813609/1‑150 (MQ=255)
                                       tGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCAGCTTTACCATCACGTTGACCAAGGATCTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTAACTACAGACGAAGGTTTACCCGCAATTtgt                                                                                                               >  2:907578/1‑150 (MQ=255)
                                         aTAACCGGAGTTGATGACATGTCCTGTATCATCTAACGCGACAATGTAGGTTTCTGCTTTACCATCACGTTGACCGAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGc                                                                                                             <  1:407616/150‑1 (MQ=255)
                                          tAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCa                                                                                                            >  1:1374021/1‑150 (MQ=255)
                                           aaCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTATGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCaa                                                                                                           <  1:1293042/150‑1 (MQ=255)
                                            aCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc                                                                                                          <  1:1339890/150‑1 (MQ=255)
                                            aCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc                                                                                                          >  2:1067210/1‑150 (MQ=255)
                                             ccGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGCCCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAcc                                                                                                         >  1:95405/1‑150 (MQ=255)
                                             ccGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGTATCATGCTCACTTAAGACGAAGGTTTACCCGCAATTTGTGCAAcc                                                                                                         >  1:1518210/1‑150 (MQ=255)
                                             ccGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAccc                                                                                                         >  1:107499/1‑150 (MQ=255)
                                                gAGTTGATGACATGTCCGGTATCATCCAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgc                                                                                                      >  1:2126807/1‑150 (MQ=255)
                                                 aGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgcg                                                                                                     <  1:918941/150‑1 (MQ=255)
                                                      atgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCgg                                                                                                >  2:1828946/1‑150 (MQ=255)
                                                       tgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGc                                                                                               >  1:2194594/1‑150 (MQ=255)
                                                       tgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGc                                                                                               <  1:117036/150‑1 (MQ=255)
                                                         aCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTc                                                                                             >  1:1783502/1‑150 (MQ=255)
                                                           aTGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAt                                                                                           >  1:1886889/1‑150 (MQ=255)
                                                                  gTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtttt                                                                                    <  1:1751495/150‑1 (MQ=255)
                                                                     tgatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTg                                                                                 <  1:1357282/148‑1 (MQ=255)
                                                                       atcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGAc                                                                               <  1:678325/150‑1 (MQ=255)
                                                                        tcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTCGGGCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGACGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACg                                                                              <  1:1457773/150‑1 (MQ=255)
                                                                         cTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGGCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGt                                                                             <  2:1357341/150‑1 (MQ=255)
                                                                          tAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTTTGCAACCTGCGCCCGGCTCATGCCTTTTTTTACTTc                                                                            >  2:2198876/1‑150 (MQ=255)
                                                                          tAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTc                                                                            <  1:592164/150‑1 (MQ=255)
                                                                           aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTGCCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCt                                                                           >  1:472728/1‑150 (MQ=255)
                                                                           aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCt                                                                           <  2:948069/150‑1 (MQ=255)
                                                                               cgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTca                                                                       <  2:225111/150‑1 (MQ=255)
                                                                                   aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTcaccac                                                                   <  2:1303698/150‑1 (MQ=255)
                                                                                    aaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTACGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACcaca                                                                  <  2:364016/150‑1 (MQ=255)
                                                                                         ggTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTg                                                             <  1:2206450/150‑1 (MQ=255)
                                                                                            ttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTAc                                                          >  2:686460/1‑150 (MQ=255)
                                                                                            ttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGAAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCCCAACAGGCGGTaa                                                          >  2:418329/1‑149 (MQ=255)
                                                                                               tGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAGGCCTTTTTTGACGTCTTTCACCCCAGGCTGTAaaaa                                                       >  2:1669717/1‑150 (MQ=255)
                                                                                                gCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAAc                                                      <  1:1290319/150‑1 (MQ=255)
                                                                                                gCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAAc                                                      <  2:1454242/150‑1 (MQ=255)
                                                                                                 cTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCTCTTCAGACGTAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCTCAGGCTGTACAAACt                                                     <  1:199110/150‑1 (MQ=255)
                                                                                                   ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTgg                                                   <  1:26825/150‑1 (MQ=255)
                                                                                                     aCCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTc                                                 >  2:89549/1‑150 (MQ=255)
                                                                                                        aTCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCttt                                              >  2:1693538/1‑150 (MQ=255)
                                                                                                        aTCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGCGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCttt                                              <  2:1450323/150‑1 (MQ=255)
                                                                                                          cACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTgg                                            <  2:1838749/150‑1 (MQ=255)
                                                                                                          cACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAATTGTGCAACCTGCGCCCGGCTCATGGCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTgg                                            <  2:287224/150‑1 (MQ=255)
                                                                                                            cGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTa                                          >  1:1868717/1‑150 (MQ=255)
                                                                                                            cGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGGCTTTGGTa                                          <  2:1720138/150‑1 (MQ=255)
                                                                                                            cGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTAACCACAGGCTGTACAAACTGGTCTTTGGTa                                          >  1:2039561/1‑150 (MQ=255)
                                                                                                            cGTTGACCAAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTa                                          <  2:2051021/150‑1 (MQ=255)
                                                                                                                  cccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAACCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCTCCACAGGCTGTACATACGGGTCTTTGGTACGATCa                                    >  2:706221/1‑150 (MQ=255)
                                                                                                                   ccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCAt                                   >  1:2068179/1‑150 (MQ=255)
                                                                                                                    cAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATa                                  >  2:1768293/1‑150 (MQ=255)
                                                                                                                      ggATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAg                                <  2:2087237/150‑1 (MQ=255)
                                                                                                                       gATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGc                               >  1:837453/1‑150 (MQ=255)
                                                                                                                          gTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGt                            >  1:610926/1‑150 (MQ=255)
                                                                                                                          gTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAGGCCTGTTTTGACGTCTTTCACAACAGGCTGTACAAACTCGTCTTTGGTACGATCATACCCCGt                            >  1:475028/1‑150 (MQ=255)
                                                                                                                            aGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCCCAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTAc                          >  2:251103/1‑150 (MQ=255)
                                                                                                                             ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTaaa                         >  2:1865840/1‑148 (MQ=255)
                                                                                                                              gTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACCAACTGGTCTTTGGTACGATCATAAGCCGTACaa                        >  1:406509/1‑150 (MQ=255)
                                                                                                                                 tGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAAccc                     <  1:733343/150‑1 (MQ=255)
                                                                                                                                  ggCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCGCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCg                    >  1:1041324/1‑150 (MQ=255)
                                                                                                                                       gTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGc               >  1:1202416/1‑150 (MQ=255)
                                                                                                                                        tACCGCGTGCATGGTTCATGCTCACTTCAGACGTATGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACACGGTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCa              <  1:1829141/150‑1 (MQ=255)
                                                                                                                                        tACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAAGTGGTCTTTGGTACGATCATAAGCCGTACAACACGCCAGCa              >  2:1549850/1‑150 (MQ=255)
                                                                                                                                         aCCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCAt             >  1:1546736/1‑150 (MQ=255)
                                                                                                                                          ccGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATg            >  1:2000274/1‑150 (MQ=255)
                                                                                                                                           cgcgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCACCCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCCCCACAGGCTGTACAAACTGGTCTTTGGTACGAGCATAACCCGTACAACCCGCCAGCATgg           >  2:153454/1‑150 (MQ=255)
                                                                                                                                           cgcgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATgg           <  1:92503/150‑1 (MQ=255)
                                                                                                                                           agtgAGCATGATCCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCCCCCTGCGCCCGGCTCATGCCTTTTTTGACGTCGTTCTCCACAGGCTGTACAAACTGGTCTTTGGTACGATCAGAACCCGTACAACCCGCCCGCAggg           >  2:582822/4‑150 (MQ=255)
                                                                                                                                           agtgAGCATGATCCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATgg           >  2:709697/4‑150 (MQ=255)
                                                                                                                                            gtgAGCATGATCCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGt          >  1:1270205/3‑150 (MQ=255)
                                                                                                                                            gcgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCTCCCGGATCATGCCTTTTTTGACTTCTTTCACGACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAGCCCGCCAGCATGGt          >  2:103229/1‑150 (MQ=255)
                                                                                                                                            gcgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGt          >  2:1171889/1‑150 (MQ=255)
                                                                                                                                             cgAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGtt         <  1:1651565/150‑1 (MQ=255)
                                                                                                                                              gAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTa        <  1:1802273/150‑1 (MQ=255)
                                                                                                                                                 cATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATa     >  1:822284/1‑150 (MQ=255)
                                                                                                                                                  aTGGATCATGCTCACTTCAGACGAATGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATAc    <  1:1435011/150‑1 (MQ=255)
                                                                                                                                                    ggATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACATGCGCCCGGCTCATGCCTTTTATGACGTCTTTCACCCCAGGCTGTACAAACTGGTCTATGGTACGATCATAAGCCGTACAAACCGCCAGCATGGTTAATAccg  >  2:2186377/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCG  >  NC_000913/1821922‑1822219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: