Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,758 G→A 7.4% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7580GA 7.4% 371.0 / 12.7 121intergenic (+24/+24)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (59/53);  new base A (4/5);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

Only 100 of 127 total aligned reads displayed.

GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907
                                                                                                                                                     |                                                                                                                                                      
gATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAAAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCggg                                                                                                                                                        <  1:228178/150‑1 (MQ=255)
   atGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGc                                                                                                                                                     <  1:748503/150‑1 (MQ=255)
    tGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCa                                                                                                                                                    >  2:417238/1‑150 (MQ=255)
    tGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCa                                                                                                                                                    >  1:1415349/1‑150 (MQ=255)
    tGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGACGGGTGCa                                                                                                                                                    >  1:2007339/1‑150 (MQ=255)
      ccGGATGATTGCTACAATACTTTTGTTTGTGTAGAATCGGCTTACGCGTCAGAAACGCAAAATGTGACGAAAGTGAAACCTGCACATATGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGGTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                  <  2:1466776/150‑1 (MQ=255)
      ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTTTTCAGGGTGCCAt                                                                                                                                                  >  2:1929249/1‑150 (MQ=255)
      ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                  >  1:1529735/1‑150 (MQ=255)
      ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                  >  2:1198872/1‑150 (MQ=255)
          atgatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAt                                                                                                                                              <  2:315420/150‑1 (MQ=255)
              tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGAAAAGCATACgg                                                                                                                                          >  2:428578/1‑150 (MQ=255)
              tgGCTACAAAACATTTATTTGTGTAGAAACGGCTTACGCATCAGAAACGCAAAAAGAGACCAAAGAGAAACCTGCACAACTGGCGCAAACCATTCGCGATGCGAAACGTGAATTTAGGTTAATGTTGTGTGCCGGGTGCAATGAATCCgc                                                                                                                                          >  2:1453163/1‑149 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       <  1:190359/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       >  1:1453066/1‑150 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                      <  1:184103/150‑1 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                      <  2:1044894/150‑1 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                      <  2:2064002/150‑1 (MQ=255)
                  tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                     >  2:956705/1‑150 (MQ=255)
                   aCAAAACATTTGTTTGTGTAGAAACTGCTTACGCTTCAGAAACGCAAAAACTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                                                                    <  1:632557/150‑1 (MQ=255)
                     aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTAGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACacaa                                                                                                                                  <  1:308263/150‑1 (MQ=255)
                      aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCATAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                 <  2:615471/150‑1 (MQ=255)
                         cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                              >  1:1536671/1‑150 (MQ=255)
                         aaTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                              <  1:1387856/149‑1 (MQ=255)
                          aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                             <  2:1026543/150‑1 (MQ=255)
                            ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                           <  2:1526775/150‑1 (MQ=255)
                             tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCATAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                                                                          >  1:320877/1‑150 (MQ=255)
                              gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                                                                         >  1:1354962/1‑150 (MQ=255)
                                    ttAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACAAAAGAGAACCTTGCACATCTGGCGCAATCCATTCGCGTGGCTAAACGTTATTTTACTTTATTGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATcc                                                                                                                   <  2:1611287/149‑1 (MQ=255)
                                     tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                  <  1:1587233/150‑1 (MQ=255)
                                         aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACCTCACACCATATCCAgcgc                                                                                                              >  2:415268/1‑150 (MQ=255)
                                         aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCAATGCAAACGGCACACAACATCACACCATATGCAgcgc                                                                                                              >  1:438392/1‑150 (MQ=255)
                                          aCGGCTTACGCTTCAGAAACGCAAAAAGTGACAAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGGGTGGCGAAACGTTAATTTACGTTAATGTGGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                             <  2:1968205/150‑1 (MQ=255)
                                            ggCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGt                                                                                                           <  2:829503/150‑1 (MQ=255)
                                             gCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtt                                                                                                          >  1:895542/1‑150 (MQ=255)
                                              cTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAAACTCACACCATATCCAGCGCAGttt                                                                                                         >  2:2146929/1‑150 (MQ=255)
                                                tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                       <  2:1504041/150‑1 (MQ=255)
                                                tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAACACACCCTATCCAGAGCAGttttt                                                                                                       >  1:1295335/1‑150 (MQ=255)
                                                 aCGCTTCAGAAACTCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAATATTTAAATTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATCCAGCGCAGTTTTTc                                                                                                      >  2:1370573/1‑150 (MQ=255)
                                                 aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                      <  2:115/150‑1 (MQ=255)
                                                    cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTACtt                                                                                                   >  1:975170/1‑150 (MQ=255)
                                                    cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAAACGGCACACACCATAACACCATATCCAGCGCAGTTTTTCCtt                                                                                                   >  2:432506/1‑150 (MQ=255)
                                                    cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATATGGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCACCCCACTATTTCCtt                                                                                                   >  2:372519/1‑150 (MQ=255)
                                                     ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                  <  2:1924394/150‑1 (MQ=255)
                                                      tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATACGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                 >  2:637490/1‑150 (MQ=255)
                                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                >  1:1256519/1‑150 (MQ=255)
                                                       aataaaCGCAAAAAGTAACCAAATAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                <  1:1058635/147‑1 (MQ=255)
                                                        aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTgg                                                                                               <  2:1484186/150‑1 (MQ=255)
                                                         gAAACGCAAAAAGTGACCAAAGGGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                              >  1:1134119/1‑150 (MQ=255)
                                                          aaaCGCAAAAAGGGACCAAAGATAAACCTGCAAATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                             <  1:2088611/150‑1 (MQ=255)
                                                           aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGc                                                                                            <  2:1092242/150‑1 (MQ=255)
                                                              gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                         <  1:1366315/150‑1 (MQ=255)
                                                               cAAAAAGTGACCAAAGAGAACCCTGCACATCTGGCGCAATCCATTCGCGATCCGCAACGTTAATTTCCGTTAATGATGTCAGCCGGGTTCAATGCATCCGGCACCCAACATCACACGATATCCAGCGCAGATTATACTGTTGGTGCCGGa                                                                                        >  1:131481/1‑150 (MQ=255)
                                                               cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACCTCACACCATTTCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                        >  2:1987783/1‑150 (MQ=255)
                                                                     gTGACCAAAGAGAAACCTGCACATCTGGCGCACTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                  <  1:250785/150‑1 (MQ=255)
                                                                        aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                               <  2:370153/150‑1 (MQ=255)
                                                                         ccAAAGAGATACCTGCACATCTGGCGCAATCCATTCGCGGTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCCATGCATCGGGCACACAACAACAACCCCTTTCACGCGGAGTTTTTAAATTTTGTGCCGGATATTCCtttt                                                                              >  1:1643248/1‑148 (MQ=255)
                                                                         ccAAAGAGAAACCTGCACATCTGGCGCTATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATCCGGCACACAACCTCAAACCATATACCGCGCAGGTTTTCCTTTTGGTGGCGGATATGCCTTAt                                                                              >  1:2114664/1‑150 (MQ=255)
                                                                         ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGCGTGCAATGCATCCGCCACACAACATAACACCATATCCAGCGAAGTTTTTCCTTTTGGTGCCGGATATTCCTTAt                                                                              >  1:267291/1‑150 (MQ=255)
                                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTGCGGGTGCAATGCATCCGGCACCCAACATAACACCAAATACAGCGCAGTTTTTACTTTTGGTGCCGGTTTAGCCTTATcc                                                                            >  2:1813380/1‑150 (MQ=255)
                                                                              gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                         >  2:2179099/1‑150 (MQ=255)
                                                                                 aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACTTTAATTTACGTTAATGTTGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATa                                                                      <  1:758441/150‑1 (MQ=255)
                                                                                  atcgtGCACATGTGGCGAAATCATTTGGGGTTGCTAAACGTTATTTTATTTAATTGTGGTGTGCGGGGGGAAATTCATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAg                                                                     <  2:2133581/146‑1 (MQ=255)
                                                                                  aaCCTGAACATCTGGCGCTATCCATTCGCGTTGCGAAACGTTAATTTACCTTAATGTTGTGTGCCGGGTGCAATGCATTCGGCACACAACATCACACGATATCGAGCGCAGTTTTTCCTTTTGGTGGCGGATATGCCTTATCCAGCATAg                                                                     <  2:90982/150‑1 (MQ=255)
                                                                                              tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                         >  2:1195423/1‑150 (MQ=255)
                                                                                                gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                       <  2:1536671/150‑1 (MQ=255)
                                                                                                 cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATAACCAGCGCAATTTTTAATTTTGGTGGCGGATATGCCTTATCCAGCAAAGCTAATTCCGATgaag                                                      >  2:1105861/1‑150 (MQ=255)
                                                                                                   cAAGCCATTCGCGGTGCGAAACGTTAATTTACGGTAATGTAGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATTTGCCTTATCCAGCATAGCTAATTCAGCTgaagaa                                                    <  1:332016/150‑1 (MQ=255)
                                                                                                     aTCCATTCGCGTTGCTAAACGTTAATTTACGTTAATTTTGTGTGCCGGGTGAAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAg                                                  <  1:854194/150‑1 (MQ=255)
                                                                                                      tCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGGAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGt                                                 <  1:298395/150‑1 (MQ=255)
                                                                                                       ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCCCACAACATCACACCATATCCAGCGCAGTTTTTCATTTTGGTGCCGGATATGCCTTATCCCGCATAGCTAATTCCGCTGAAGAAAGtt                                                >  2:1013162/1‑150 (MQ=255)
                                                                                                         aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                              <  1:7027/150‑1 (MQ=255)
                                                                                                             ccgTTGCGAAACGTTAATTTACGTTAATGTTGGTGGGCGGGTGCAATGGATCCGGCAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                           <  1:417238/149‑1 (MQ=255)
                                                                                                                 tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                      >  1:197623/1‑150 (MQ=255)
                                                                                                                    gAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                   >  2:546637/1‑150 (MQ=255)
                                                                                                                            aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAt                           >  1:1455746/1‑150 (MQ=255)
                                                                                                                              tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                         <  2:112255/150‑1 (MQ=255)
                                                                                                                              tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                         <  1:1518097/150‑1 (MQ=255)
                                                                                                                                 aCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgt                      <  1:1795536/150‑1 (MQ=255)
                                                                                                                                  cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTTCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                     >  2:1235402/1‑150 (MQ=255)
                                                                                                                                  cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATTCCTTATACAGCATAGCTAATTCCGCTGAACAAAGTTCGACCTCAAGCACCGCCGCATTttgtt                     >  2:2169078/1‑150 (MQ=255)
                                                                                                                                  cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                     >  1:1242778/1‑150 (MQ=255)
                                                                                                                                   gTTAATGTTGTGTGCCGGGTGCAATTCATCCGGCACACTACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATACCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCAGCATTttgttg                    <  1:392119/150‑1 (MQ=255)
                                                                                                                                   gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACTTCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                    <  1:2118659/150‑1 (MQ=255)
                                                                                                                                    ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                   <  2:1529735/150‑1 (MQ=255)
                                                                                                                                    ttAATGTTGTGGGCCGGGTCCAATGAAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                   <  1:1662887/150‑1 (MQ=255)
                                                                                                                                        tgttgtGTGCCGGGTGCAATTCATACGGCACACAACATCACACCAAATACAGCGCAGTTTTTACTTTTTGTGGCGGATATGCCATTTACAGCATAGCTAATTACGCTGAAGAAAGTTAGACCTCAATCACAGCCGCATTTTTTTGGaaaa               >  2:1921276/1‑147 (MQ=255)
                                                                                                                                        tgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt               >  1:1428225/1‑150 (MQ=255)
                                                                                                                                        tgttgtGTGCAGGGTGCAATGCATCCGGAACAAAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt               <  2:1337631/150‑1 (MQ=255)
                                                                                                                                         gttgtGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCTGATTTGCCTTATCCAGCTTAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATg              <  2:1358601/150‑1 (MQ=255)
                                                                                                                                            gtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACCTGGGc           >  2:17070/1‑150 (MQ=255)
                                                                                                                                              gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTTGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa         >  1:940189/1‑150 (MQ=255)
                                                                                                                                              gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa         >  1:593491/1‑150 (MQ=255)
                                                                                                                                              gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGcta         >  2:1478121/1‑148 (MQ=255)
                                                                                                                                              gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa         >  2:1175844/1‑150 (MQ=255)
                                                                                                                                               tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt        >  2:2101372/1‑150 (MQ=255)
                                                                                                                                                gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc       >  2:1777162/1‑150 (MQ=255)
                                                                                                                                                  cGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGt     >  2:485944/1‑150 (MQ=255)
                                                                                                                                                    ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg   <  2:1262911/150‑1 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  1:1198872/150‑1 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  1:1133099/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: