Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,763 A→T 7.1% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7630AT 7.1% 346.9 / 10.2 123intergenic (+29/+19)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (58/56);  new base T (4/5);  total (62/61)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 125 total aligned reads displayed.

CCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCT  >  NC_000913/1864615‑1864911
                                                                                                                                                    |                                                                                                                                                    
ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTTTTCAGGGTGCCAt                                                                                                                                                     >  2:1929249/1‑150 (MQ=255)
ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                     >  2:1198872/1‑150 (MQ=255)
ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                     >  1:1529735/1‑150 (MQ=255)
ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGATGTGTGCCGGGTGCAAt                                                                                                                                                     >  2:1495294/1‑150 (MQ=255)
 cGGATGAGGGCTACAAAACATTTGTGTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGACACCTTCACATCTGGCGCAATCCATTCGCGTTGCGAAACGGTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                    <  1:207580/150‑1 (MQ=255)
    atgatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAt                                                                                                                                                 <  2:315420/150‑1 (MQ=255)
        tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGAAAAGCATACgg                                                                                                                                             >  2:428578/1‑150 (MQ=255)
        tgGCTACAAAACATTTATTTGTGTAGAAACGGCTTACGCATCAGAAACGCAAAAAGAGACCAAAGAGAAACCTGCACAACTGGCGCAAACCATTCGCGATGCGAAACGTGAATTTAGGTTAATGTTGTGTGCCGGGTGCAATGAATCCgc                                                                                                                                             >  2:1453163/1‑149 (MQ=255)
          gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                           >  1:1453066/1‑150 (MQ=255)
           cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                          <  2:1044894/150‑1 (MQ=255)
           cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                          <  1:184103/150‑1 (MQ=255)
            tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                         >  2:956705/1‑150 (MQ=255)
                aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCATAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                     <  2:615471/150‑1 (MQ=255)
                aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACacaac                                                                                                                                     <  2:1538424/150‑1 (MQ=255)
                   cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                                  >  1:1536671/1‑150 (MQ=255)
                   aaTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                                  <  1:1387856/149‑1 (MQ=255)
                    aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                                 <  2:1026543/150‑1 (MQ=255)
                       tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCATAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                                                                              >  1:320877/1‑150 (MQ=255)
                        gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                                                                             >  1:1354962/1‑150 (MQ=255)
                              ttAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACAAAAGAGAACCTTGCACATCTGGCGCAATCCATTCGCGTGGCTAAACGTTATTTTACTTTATTGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATcc                                                                                                                       <  2:1611287/149‑1 (MQ=255)
                              gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACCACATCACACCAAATcc                                                                                                                       >  2:2119716/1‑150 (MQ=255)
                              gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATcc                                                                                                                       >  1:634792/1‑150 (MQ=255)
                               tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACATCACACCATTTCCa                                                                                                                      >  2:1097275/1‑150 (MQ=255)
                               tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                      <  1:1587233/150‑1 (MQ=255)
                                   aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACCTCACACCATATCCAgcgc                                                                                                                  >  2:415268/1‑150 (MQ=255)
                                   aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCAATGCAAACGGCACACAACATCACACCATATGCAgcgc                                                                                                                  >  1:438392/1‑150 (MQ=255)
                                    aCGGCTTACGCTTCAGAAACGCAAAAAGTGACAAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGGGTGGCGAAACGTTAATTTACGTTAATGTGGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                                 <  2:1968205/150‑1 (MQ=255)
                                      ggCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGt                                                                                                               <  2:829503/150‑1 (MQ=255)
                                       gCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtt                                                                                                              >  1:895542/1‑150 (MQ=255)
                                        cTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAAACTCACACCATATCCAGCGCAGttt                                                                                                             >  2:2146929/1‑150 (MQ=255)
                                          tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                           <  2:1504041/150‑1 (MQ=255)
                                          tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAACACACCCTATCCAGAGCAGttttt                                                                                                           >  1:1295335/1‑150 (MQ=255)
                                           aCGCTTCAGAAACTCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAATATTTAAATTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATCCAGCGCAGTTTTTc                                                                                                          >  2:1370573/1‑150 (MQ=255)
                                           aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                          <  2:115/150‑1 (MQ=255)
                                             gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCt                                                                                                        >  2:1860842/1‑150 (MQ=255)
                                              cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                       >  2:1186879/1‑150 (MQ=255)
                                              cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTACtt                                                                                                       >  1:975170/1‑150 (MQ=255)
                                              cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAAACGGCACACACCATAACACCATATCCAGCGCAGTTTTTCCtt                                                                                                       >  2:432506/1‑150 (MQ=255)
                                              cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATATGGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCACCCCACTATTTCCtt                                                                                                       >  2:372519/1‑150 (MQ=255)
                                                tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATACGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                     >  2:637490/1‑150 (MQ=255)
                                                 cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                    >  1:1256519/1‑150 (MQ=255)
                                                 aataaaCGCAAAAAGTAACCAAATAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                    <  1:1058635/147‑1 (MQ=255)
                                                  aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTgg                                                                                                   <  2:1484186/150‑1 (MQ=255)
                                                   gAAACGCAAAAAGTGACCAAAGGGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                                  >  1:1134119/1‑150 (MQ=255)
                                                    aaaCGCAAAAAGGGACCAAAGATAAACCTGCAAATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                                 <  1:2088611/150‑1 (MQ=255)
                                                     aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGc                                                                                                <  2:1092242/150‑1 (MQ=255)
                                                        gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                             <  1:1366315/150‑1 (MQ=255)
                                                         cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACCTCACACCATTTCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                            >  2:1987783/1‑150 (MQ=255)
                                                         cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                            >  2:1267846/1‑150 (MQ=255)
                                                              aGTCACCAAACAGAAACCTGCACTTCTGGCGCAATCAATTCGCGTTGCTAAACTTAAATTTACTTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                       <  1:1866760/150‑1 (MQ=255)
                                                               gTGACCAAAGAGAAACCTGCACATCTGGCGCACTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                      <  1:250785/150‑1 (MQ=255)
                                                                   ccAAAGAGAAACCTGCACATCTGGCGCTATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATCCGGCACACAACCTCAAACCATATACCGCGCAGGTTTTCCTTTTGGTGGCGGATATGCCTTAt                                                                                  >  1:2114664/1‑150 (MQ=255)
                                                                   ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGCGTGCAATGCATCCGCCACACAACATAACACCATATCCAGCGAAGTTTTTCCTTTTGGTGCCGGATATTCCTTAt                                                                                  >  1:267291/1‑150 (MQ=255)
                                                                     aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTGCGGGTGCAATGCATCCGGCACCCAACATAACACCAAATACAGCGCAGTTTTTACTTTTGGTGCCGGTTTAGCCTTATcc                                                                                >  2:1813380/1‑150 (MQ=255)
                                                                        gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                             >  2:2179099/1‑150 (MQ=255)
                                                                           aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACTTTAATTTACGTTAATGTTGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATa                                                                          <  1:758441/150‑1 (MQ=255)
                                                                           aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATa                                                                          <  2:1453066/150‑1 (MQ=255)
                                                                            atcgtGCACATGTGGCGAAATCATTTGGGGTTGCTAAACGTTATTTTATTTAATTGTGGTGTGCGGGGGGAAATTCATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAg                                                                         <  2:2133581/146‑1 (MQ=255)
                                                                            aaCCTGAACATCTGGCGCTATCCATTCGCGTTGCGAAACGTTAATTTACCTTAATGTTGTGTGCCGGGTGCAATGCATTCGGCACACAACATCACACGATATCGAGCGCAGTTTTTCCTTTTGGTGGCGGATATGCCTTATCCAGCATAg                                                                         <  2:90982/150‑1 (MQ=255)
                                                                              ccTGCACATCTGGCGCATTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                       <  1:318251/150‑1 (MQ=255)
                                                                              ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                       <  1:122288/150‑1 (MQ=255)
                                                                                          gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                           <  2:1536671/150‑1 (MQ=255)
                                                                                             cAAGCCATTCGCGGTGCGAAACGTTAATTTACGGTAATGTAGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATTTGCCTTATCCAGCATAGCTAATTCAGCTgaagaa                                                        <  1:332016/150‑1 (MQ=255)
                                                                                               aTCCATTCGCGTTGCTAAACGTTAATTTACGTTAATTTTGTGTGCCGGGTGAAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAg                                                      <  1:854194/150‑1 (MQ=255)
                                                                                               aTCCATTCGCGTTGCGAATCTTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGATAg                                                      <  1:249944/150‑1 (MQ=255)
                                                                                                tCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGGAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGt                                                     <  1:298395/150‑1 (MQ=255)
                                                                                                 ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCCCACAACATCACACCATATCCAGCGCAGTTTTTCATTTTGGTGCCGGATATGCCTTATCCCGCATAGCTAATTCCGCTGAAGAAAGtt                                                    >  2:1013162/1‑150 (MQ=255)
                                                                                                  cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc                                                   <  1:428968/150‑1 (MQ=255)
                                                                                                   aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                                  <  1:7027/150‑1 (MQ=255)
                                                                                                        cgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACCCCAAATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAACTTCGACCTc                                             >  1:1354111/1‑150 (MQ=255)
                                                                                                           tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                          >  1:197623/1‑150 (MQ=255)
                                                                                                            gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                         <  1:1495294/150‑1 (MQ=255)
                                                                                                              gAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                       >  2:546637/1‑150 (MQ=255)
                                                                                                                      aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAt                               >  1:1455746/1‑150 (MQ=255)
                                                                                                                        tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                             <  2:112255/150‑1 (MQ=255)
                                                                                                                           aCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgt                          <  1:1795536/150‑1 (MQ=255)
                                                                                                                            cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTTCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                         >  2:1235402/1‑150 (MQ=255)
                                                                                                                            cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                         >  1:1242778/1‑150 (MQ=255)
                                                                                                                             gTTAATGTTGTGTGCCGGGTGCAATTCATCCGGCACACTACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATACCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCAGCATTttgttg                        <  1:392119/150‑1 (MQ=255)
                                                                                                                              ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                       <  2:1529735/150‑1 (MQ=255)
                                                                                                                              ttAATGTTGTGGGCCGGGTCCAATGAAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                       <  1:1662887/150‑1 (MQ=255)
                                                                                                                                  tgttgtGTGCCGGGTGCAATTCATACGGCACACAACATCACACCAAATACAGCGCAGTTTTTACTTTTTGTGGCGGATATGCCATTTACAGCATAGCTAATTACGCTGAAGAAAGTTAGACCTCAATCACAGCCGCATTTTTTTGGaaaa                   >  2:1921276/1‑147 (MQ=255)
                                                                                                                                  tgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                   >  1:1428225/1‑150 (MQ=255)
                                                                                                                                   gttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATg                  >  2:2211331/1‑150 (MQ=255)
                                                                                                                                   gttgtGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCTGATTTGCCTTATCCAGCTTAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATg                  <  2:1358601/150‑1 (MQ=255)
                                                                                                                                       tgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa              >  2:1940271/1‑150 (MQ=255)
                                                                                                                                        gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTTGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa             >  1:940189/1‑150 (MQ=255)
                                                                                                                                        gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa             >  1:593491/1‑150 (MQ=255)
                                                                                                                                        gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGcta             >  2:1478121/1‑148 (MQ=255)
                                                                                                                                        gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa             >  2:1175844/1‑150 (MQ=255)
                                                                                                                                         tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt            >  2:2101372/1‑150 (MQ=255)
                                                                                                                                          gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc           >  2:1777162/1‑150 (MQ=255)
                                                                                                                                            cGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGt         >  2:485944/1‑150 (MQ=255)
                                                                                                                                              ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg       <  2:1262911/150‑1 (MQ=255)
                                                                                                                                               gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc      <  1:1133099/150‑1 (MQ=255)
                                                                                                                                               gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc      <  1:1602974/150‑1 (MQ=255)
                                                                                                                                                tcaaaTGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGcc     <  2:232736/147‑1 (MQ=255)
                                                                                                                                                 gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCg    >  1:1072637/1‑150 (MQ=255)
                                                                                                                                                 gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAGTTTGTTGGACATGGGCAATCGTGGCCg    >  1:451791/1‑150 (MQ=255)
                                                                                                                                                   aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCt  >  2:775243/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCT  >  NC_000913/1864615‑1864911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: