Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,768 T→C 7.3% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7680TC 7.3% 338.0 / 14.3 126intergenic (+34/+14)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (53/61);  new base C (4/5);  total (59/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-02

Only 100 of 128 total aligned reads displayed.

ATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGA  >  NC_000913/1864619‑1864917
                                                                                                                                                     |                                                                                                                                                      
atgatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAt                                                                                                                                                        <  2:315420/150‑1 (MQ=255)
    tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGAAAAGCATACgg                                                                                                                                                    >  2:428578/1‑150 (MQ=255)
    tgGCTACAAAACATTTATTTGTGTAGAAACGGCTTACGCATCAGAAACGCAAAAAGAGACCAAAGAGAAACCTGCACAACTGGCGCAAACCATTCGCGATGCGAAACGTGAATTTAGGTTAATGTTGTGTGCCGGGTGCAATGAATCCgc                                                                                                                                                    >  2:1453163/1‑149 (MQ=255)
     ggCTACAAAACATTTGTTTGTGTAGAAACTGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGATAAACCTGCAGATCTGGCGCAATCGATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                                   <  2:1021538/150‑1 (MQ=255)
       cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                                <  2:2064002/150‑1 (MQ=255)
       cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                                <  1:184103/150‑1 (MQ=255)
        tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                               >  2:956705/1‑150 (MQ=255)
         aCAAAACATTTGTTTGTGTAGAAACTGCTTACGCTTCAGAAACGCAAAAACTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                                                                              <  1:632557/150‑1 (MQ=255)
            aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCATAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                           <  2:615471/150‑1 (MQ=255)
            aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACacaac                                                                                                                                           <  2:1538424/150‑1 (MQ=255)
               cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                                        >  1:1536671/1‑150 (MQ=255)
               aaTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                                        <  1:1387856/149‑1 (MQ=255)
                aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                                       <  2:1026543/150‑1 (MQ=255)
                  ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                                     <  2:1526775/150‑1 (MQ=255)
                   tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCATAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                                                                                    >  1:320877/1‑150 (MQ=255)
                          ttAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACAAAAGAGAACCTTGCACATCTGGCGCAATCCATTCGCGTGGCTAAACGTTATTTTACTTTATTGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATcc                                                                                                                             <  2:1611287/149‑1 (MQ=255)
                          gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACCACATCACACCAAATcc                                                                                                                             >  2:2119716/1‑150 (MQ=255)
                          gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATcc                                                                                                                             >  1:634792/1‑150 (MQ=255)
                           tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACATCACACCATTTCCa                                                                                                                            >  2:1097275/1‑150 (MQ=255)
                           tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                            <  1:1587233/150‑1 (MQ=255)
                               aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCAATGCAAACGGCACACAACATCACACCATATGCAgcgc                                                                                                                        >  1:438392/1‑150 (MQ=255)
                                aCGGCTTACGCTTCAGAAACGCAAAAAGTGACAAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGGGTGGCGAAACGTTAATTTACGTTAATGTGGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                                       <  2:1968205/150‑1 (MQ=255)
                                  ggCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGt                                                                                                                     <  2:829503/150‑1 (MQ=255)
                                   gCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtt                                                                                                                    >  1:895542/1‑150 (MQ=255)
                                    cTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAAACTCACACCATATCCAGCGCAGttt                                                                                                                   >  2:2146929/1‑150 (MQ=255)
                                      tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                                 <  2:1504041/150‑1 (MQ=255)
                                      tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAACACACCCTATCCAGAGCAGttttt                                                                                                                 >  1:1295335/1‑150 (MQ=255)
                                       aCGCTTCAGAAACTCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAATATTTAAATTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATCCAGCGCAGTTTTTc                                                                                                                >  2:1370573/1‑150 (MQ=255)
                                          cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                             >  2:1186879/1‑150 (MQ=255)
                                          cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAAACGGCACACACCATAACACCATATCCAGCGCAGTTTTTCCtt                                                                                                             >  2:432506/1‑150 (MQ=255)
                                          cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATATGGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCACCCCACTATTTCCtt                                                                                                             >  2:372519/1‑150 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                            <  2:1924394/150‑1 (MQ=255)
                                            tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATACGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                           >  2:637490/1‑150 (MQ=255)
                                             cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                          >  1:1256519/1‑150 (MQ=255)
                                             aataaaCGCAAAAAGTAACCAAATAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                          <  1:1058635/147‑1 (MQ=255)
                                              aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTgg                                                                                                         <  2:1484186/150‑1 (MQ=255)
                                               gAAACGCAAAAAGTGACCAAAGGGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                                        >  1:1134119/1‑150 (MQ=255)
                                                aaaCGCAAAAAGGGACCAAAGATAAACCTGCAAATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                                       <  1:2088611/150‑1 (MQ=255)
                                                 aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGc                                                                                                      <  2:1092242/150‑1 (MQ=255)
                                                    gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                                   <  1:1366315/150‑1 (MQ=255)
                                                     cAAAAAGTGACCAAAGAGAACCCTGCACATCTGGCGCAATCCATTCGCGATCCGCAACGTTAATTTCCGTTAATGATGTCAGCCGGGTTCAATGCATCCGGCACCCAACATCACACGATATCCAGCGCAGATTATACTGTTGGTGCCGGa                                                                                                  >  1:131481/1‑150 (MQ=255)
                                                     cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACCTCACACCATTTCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                                  >  2:1987783/1‑150 (MQ=255)
                                                     cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                                  >  2:1267846/1‑150 (MQ=255)
                                                          aGTCACCAAACAGAAACCTGCACTTCTGGCGCAATCAATTCGCGTTGCTAAACTTAAATTTACTTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                             <  1:1866760/150‑1 (MQ=255)
                                                           gTGACCAAAGAGAAACCTGCACATCTGGCGCACTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                            <  1:250785/150‑1 (MQ=255)
                                                              aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                                         <  2:370153/150‑1 (MQ=255)
                                                               ccAAAGAGATACCTGCACATCTGGCGCAATCCATTCGCGGTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCCATGCATCGGGCACACAACAACAACCCCTTTCACGCGGAGTTTTTAAATTTTGTGCCGGATATTCCtttt                                                                                        >  1:1643248/1‑148 (MQ=255)
                                                               ccAAAGAGAAACCTGCACATCTGGCGCTATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATCCGGCACACAACCTCAAACCATATACCGCGCAGGTTTTCCTTTTGGTGGCGGATATGCCTTAt                                                                                        >  1:2114664/1‑150 (MQ=255)
                                                               ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGCGTGCAATGCATCCGCCACACAACATAACACCATATCCAGCGAAGTTTTTCCTTTTGGTGCCGGATATTCCTTAt                                                                                        >  1:267291/1‑150 (MQ=255)
                                                                 aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTGCGGGTGCAATGCATCCGGCACCCAACATAACACCAAATACAGCGCAGTTTTTACTTTTGGTGCCGGTTTAGCCTTATcc                                                                                      >  2:1813380/1‑150 (MQ=255)
                                                                    gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                                   >  2:2179099/1‑150 (MQ=255)
                                                                    gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTCCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                                   <  1:879498/150‑1 (MQ=255)
                                                                       aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATa                                                                                <  2:1453066/150‑1 (MQ=255)
                                                                        aaCCTGAACATCTGGCGCTATCCATTCGCGTTGCGAAACGTTAATTTACCTTAATGTTGTGTGCCGGGTGCAATGCATTCGGCACACAACATCACACGATATCGAGCGCAGTTTTTCCTTTTGGTGGCGGATATGCCTTATCCAGCATAg                                                                               <  2:90982/150‑1 (MQ=255)
                                                                          ccTGCACATCTGGCGCATTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                             <  1:318251/150‑1 (MQ=255)
                                                                          ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                             <  1:122288/150‑1 (MQ=255)
                                                                                    tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                                   >  2:1195423/1‑150 (MQ=255)
                                                                                      gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                                 <  2:1536671/150‑1 (MQ=255)
                                                                                       cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATAACCAGCGCAATTTTTAATTTTGGTGGCGGATATGCCTTATCCAGCAAAGCTAATTCCGATgaag                                                                >  2:1105861/1‑150 (MQ=255)
                                                                                         cAAGCCATTCGCGGTGCGAAACGTTAATTTACGGTAATGTAGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATTTGCCTTATCCAGCATAGCTAATTCAGCTgaagaa                                                              <  1:332016/150‑1 (MQ=255)
                                                                                           aTCCATTCGCGTTGCTAAACGTTAATTTACGTTAATTTTGTGTGCCGGGTGAAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAg                                                            <  1:854194/150‑1 (MQ=255)
                                                                                           aTCCATTCGCGTTGCGAATCTTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGATAg                                                            <  1:249944/150‑1 (MQ=255)
                                                                                            tCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGGAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGt                                                           <  1:298395/150‑1 (MQ=255)
                                                                                             ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCCCACAACATCACACCATATCCAGCGCAGTTTTTCATTTTGGTGCCGGATATGCCTTATCCCGCATAGCTAATTCCGCTGAAGAAAGtt                                                          >  2:1013162/1‑150 (MQ=255)
                                                                                              cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc                                                         <  1:428968/150‑1 (MQ=255)
                                                                                               aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                                        <  1:7027/150‑1 (MQ=255)
                                                                                                   ccgTTGCGAAACGTTAATTTACGTTAATGTTGGTGGGCGGGTGCAATGGATCCGGCAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                                     <  1:417238/149‑1 (MQ=255)
                                                                                                    cgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACCCCAAATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAACTTCGACCTc                                                   >  1:1354111/1‑150 (MQ=255)
                                                                                                     gTTGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                                  <  2:450185/150‑1 (MQ=255)
                                                                                                      ttGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCaa                                                 <  1:1422351/150‑1 (MQ=255)
                                                                                                       tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                                >  1:197623/1‑150 (MQ=255)
                                                                                                        gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                               <  1:1495294/150‑1 (MQ=255)
                                                                                                          gAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                             >  2:546637/1‑150 (MQ=255)
                                                                                                                  aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAt                                     >  1:1455746/1‑150 (MQ=255)
                                                                                                                    tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                                   <  1:1518097/150‑1 (MQ=255)
                                                                                                                    tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                                   <  2:112255/150‑1 (MQ=255)
                                                                                                                       aCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgt                                <  1:1795536/150‑1 (MQ=255)
                                                                                                                        cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTTCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                               >  2:1235402/1‑150 (MQ=255)
                                                                                                                        cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATTCCTTATACAGCATAGCTAATTCCGCTGAACAAAGTTCGACCTCAAGCACCGCCGCATTttgtt                               >  2:2169078/1‑150 (MQ=255)
                                                                                                                        cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                               >  1:1242778/1‑150 (MQ=255)
                                                                                                                              tgttgtGTGCCGGGTGCAATTCATACGGCACACAACATCACACCAAATACAGCGCAGTTTTTACTTTTTGTGGCGGATATGCCATTTACAGCATAGCTAATTACGCTGAAGAAAGTTAGACCTCAATCACAGCCGCATTTTTTTGGaaaa                         >  2:1921276/1‑147 (MQ=255)
                                                                                                                              tgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                         >  1:1428225/1‑150 (MQ=255)
                                                                                                                              tgttgtGTGCAGGGTGCAATGCATCCGGAACAAAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                         <  2:1337631/150‑1 (MQ=255)
                                                                                                                               gttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATg                        >  2:767982/1‑150 (MQ=255)
                                                                                                                                  gtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACCTGGGc                     >  2:17070/1‑150 (MQ=255)
                                                                                                                                   tgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa                    >  2:1940271/1‑150 (MQ=255)
                                                                                                                                    gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTTGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa                   >  1:940189/1‑150 (MQ=255)
                                                                                                                                    gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa                   >  2:1175844/1‑150 (MQ=255)
                                                                                                                                     tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt                  >  2:2101372/1‑150 (MQ=255)
                                                                                                                                      gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc                 >  2:1777162/1‑150 (MQ=255)
                                                                                                                                           gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc            <  1:1602974/150‑1 (MQ=255)
                                                                                                                                           gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc            <  1:1133099/150‑1 (MQ=255)
                                                                                                                                            tcaaaTGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGcc           <  2:232736/147‑1 (MQ=255)
                                                                                                                                             gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCg          >  1:1072637/1‑150 (MQ=255)
                                                                                                                                             gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAGTTTGTTGGACATGGGCAATCGTGGCCg          >  1:451791/1‑150 (MQ=255)
                                                                                                                                                  tcATCCGGGACACAAAATCATACCATTTCCAGCGCCTTTTTTCCTTTTTGTGCCGGATAAGCCTTATCCCTCCTAGCTAATTCCGCTGTACAAAGTTCGTCCTCAACCACAGCCGCATTTTGTTGGTCATTGGCAATCGTGGCCGCtttt     <  2:1557409/149‑1 (MQ=255)
                                                                                                                                                    aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg   <  1:1221203/150‑1 (MQ=255)
                                                                                                                                                    aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg   <  2:1354962/150‑1 (MQ=255)
                                                                                                                                                    aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg   <  2:1415349/150‑1 (MQ=255)
                                                                                                                                                    aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg   <  1:202541/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGA  >  NC_000913/1864619‑1864917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: