Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,929,016 A→G 6.0% intergenic (‑177/+32) ptrB ← / ← yebE protease II/DUF533 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,929,0160AG 6.0% 301.4 / 7.5 99intergenic (‑177/+32)ptrB/yebEprotease II/DUF533 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (40/53);  new base G (2/4);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 101 total aligned reads displayed.

TTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAAT  >  NC_000913/1928867‑1929163
                                                                                                                                                     |                                                                                                                                                   
ttATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGaaa                                                                                                                                                     <  2:303548/150‑1 (MQ=255)
 tataAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAAt                                                                                                                                                    >  1:2097035/1‑150 (MQ=255)
   taAGGGTGGCAAAACTCACCTGGGATGCAAGCGAAACAGGGGATTCATTGCTTATATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAg                                                                                                                                                  <  2:700752/150‑1 (MQ=255)
     aGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTc                                                                                                                                                <  2:432503/150‑1 (MQ=255)
      gggTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGTGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCa                                                                                                                                               <  1:1345826/150‑1 (MQ=255)
          ggCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACtt                                                                                                                                           <  2:1304153/150‑1 (MQ=255)
           gCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTc                                                                                                                                          >  1:1568492/1‑150 (MQ=255)
            cAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCa                                                                                                                                         <  2:1745650/150‑1 (MQ=255)
             aaaaCTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAg                                                                                                                                        <  1:581064/150‑1 (MQ=255)
             aaaaCTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAg                                                                                                                                        >  1:969873/1‑150 (MQ=255)
                    aCCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATcc                                                                                                                                 <  1:2098095/150‑1 (MQ=39)
                     ccGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCa                                                                                                                                <  1:1606012/150‑1 (MQ=39)
                     ccGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCa                                                                                                                                <  1:2098996/150‑1 (MQ=255)
                     ccGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTCTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCa                                                                                                                                <  1:1970016/150‑1 (MQ=38)
                      cGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGGCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCag                                                                                                                               <  2:317765/150‑1 (MQ=38)
                        gggATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCagag                                                                                                                             <  1:1907894/150‑1 (MQ=255)
                            tGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCg                                                                                                                         >  2:919981/1‑150 (MQ=255)
                            tGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCg                                                                                                                         <  1:28394/150‑1 (MQ=255)
                             gCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCCGGCTATCCAGAGAGCgg                                                                                                                        <  1:2078347/150‑1 (MQ=255)
                             gCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCgg                                                                                                                        <  2:1985523/150‑1 (MQ=255)
                             gCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCgg                                                                                                                        >  2:1411428/1‑150 (MQ=255)
                              cAAGCGAAACAGGGGAGTCATTGCTTAGATGTTGACAGGTAATGGCGCCGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTTTGCGATTCTGGATAGCCCGAAATAGTCTACTTCAGTCTAACAATAGAGCTGa                                                                                                                       >  1:323110/1‑150 (MQ=255)
                              cAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGa                                                                                                                       >  1:1394979/1‑150 (MQ=255)
                              cAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGa                                                                                                                       <  1:2078934/150‑1 (MQ=255)
                                aGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCACCTTCAGGCTATCCAGAGAGCGGAat                                                                                                                     >  1:340978/1‑150 (MQ=255)
                                aGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAat                                                                                                                     >  2:1389066/1‑150 (MQ=255)
                                 gCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAatt                                                                                                                    >  1:1224033/1‑150 (MQ=255)
                                   gAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATAGAATGATATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGAATCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAattat                                                                                                                  >  1:820943/1‑150 (MQ=255)
                                      aCAGGGGAGTCATTGCTTAGATGATGACATGTAATGGCTCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATGCTGGATAGCCCGAAATAGTCCACTTCAGGCTATCCAGAGAGCGGAATTATTcc                                                                                                               <  2:916783/150‑1 (MQ=255)
                                       cAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCg                                                                                                              <  2:1660104/150‑1 (MQ=255)
                                           ggAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCaa                                                                                                          <  1:645098/150‑1 (MQ=255)
                                                 aTTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCg                                                                                                    >  1:1824457/1‑150 (MQ=255)
                                                    gCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGttt                                                                                                 >  1:1722661/1‑150 (MQ=255)
                                                     cTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGtttt                                                                                                >  1:514435/1‑150 (MQ=255)
                                                     cTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGtttt                                                                                                >  1:593191/1‑150 (MQ=255)
                                                        aGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGc                                                                                             <  2:2012897/150‑1 (MQ=255)
                                                              tgaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCg                                                                                       <  2:953364/150‑1 (MQ=255)
                                                              tgaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCg                                                                                       <  2:1225170/150‑1 (MQ=255)
                                                               gaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCga                                                                                      <  2:1212695/150‑1 (MQ=255)
                                                               gaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCga                                                                                      <  1:775853/150‑1 (MQ=255)
                                                                aCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCgag                                                                                     <  1:1905725/150‑1 (MQ=255)
                                                                aCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCgag                                                                                     <  1:1981134/150‑1 (MQ=255)
                                                                 cAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCgaga                                                                                    >  2:2111380/1‑150 (MQ=255)
                                                                  aGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGAt                                                                                   >  1:587707/1‑150 (MQ=255)
                                                                  aGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTACCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTGGCTGTTCGAGAt                                                                                   <  1:1054728/150‑1 (MQ=255)
                                                                   ggTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATc                                                                                  >  1:1080767/1‑150 (MQ=255)
                                                                         ggCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTc                                                                            <  1:2057208/150‑1 (MQ=255)
                                                                         ggCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTc                                                                            >  2:7838/1‑150 (MQ=255)
                                                                         ggCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTCCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTc                                                                            <  2:1851927/150‑1 (MQ=255)
                                                                          gcgcGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCa                                                                           <  2:1183124/150‑1 (MQ=255)
                                                                          gcgcGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCa                                                                           <  2:486178/150‑1 (MQ=255)
                                                                           cgcgGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCaa                                                                          >  1:857511/1‑150 (MQ=255)
                                                                           cgcgGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAACGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCaa                                                                          >  1:1305853/1‑150 (MQ=255)
                                                                               gATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGcc                                                                      <  2:1007982/150‑1 (MQ=255)
                                                                                 tatCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGt                                                                    <  2:595705/150‑1 (MQ=255)
                                                                                   tCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCa                                                                  <  2:1824457/150‑1 (MQ=255)
                                                                                    cGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCAc                                                                 >  2:1753814/1‑150 (MQ=255)
                                                                                    cGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCAc                                                                 >  2:1501847/1‑150 (MQ=255)
                                                                                     gAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACg                                                                >  2:1143402/1‑150 (MQ=255)
                                                                                        tGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCTCGTTCAATGCCGTCACGAAc                                                             >  2:1373294/1‑150 (MQ=255)
                                                                                           tATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATc                                                          >  2:752819/1‑150 (MQ=255)
                                                                                            aTGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGCTCGCGTTCAGTGCCGTCACGAACATcc                                                         >  2:575719/1‑150 (MQ=255)
                                                                                             tGCAGACAGAGAAAATCAGCCTGTTCTAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCt                                                        <  2:2097035/150‑1 (MQ=255)
                                                                                              gCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTg                                                       <  2:1221582/150‑1 (MQ=255)
                                                                                               cagacagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgg                                                      <  2:1378357/150‑1 (MQ=255)
                                                                                               cagacagaGAAAATCAGACTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCCTTTTTGCAGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgg                                                      >  1:375849/1‑150 (MQ=255)
                                                                                                 gacagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAAATATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgggg                                                    <  1:67060/150‑1 (MQ=255)
                                                                                                   cagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGa                                                  <  1:845000/150‑1 (MQ=255)
                                                                                                    agagAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGACAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAt                                                 >  2:1863784/1‑150 (MQ=255)
                                                                                                     gagaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAtt                                                >  1:988315/1‑150 (MQ=255)
                                                                                                     gagaAAAACAGCCTGTTCAAATGGCTGTGAGATTCTGGATAGACCGAAATAGTCAACTTCAGGCTATACAGAGAGCGGAATTATTCCTCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAtt                                                >  2:939117/1‑150 (MQ=255)
                                                                                                      agaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAttt                                               >  2:1274128/1‑150 (MQ=255)
                                                                                                       gaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTTCTGTTCGAGATCGCGTTCAATGCCGTCACGAAAATCCTGGGGGAtttt                                              >  1:1766566/1‑150 (MQ=255)
                                                                                                        aaaaTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTc                                             <  2:2091994/150‑1 (MQ=255)
                                                                                                        aaaaTCAGCCTGTACAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTACGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTc                                             <  1:986254/150‑1 (MQ=255)
                                                                                                            tCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAgcg                                         <  1:1039955/150‑1 (MQ=255)
                                                                                                             cAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAgcgc                                        >  2:813024/1‑150 (MQ=255)
                                                                                                              aGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGcgcg                                       <  2:1879405/150‑1 (MQ=255)
                                                                                                                       aaaTGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGt                              >  1:713017/1‑150 (MQ=255)
                                                                                                                        aaTGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTg                             <  1:1610820/150‑1 (MQ=255)
                                                                                                                          tGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCa                           <  2:1394979/150‑1 (MQ=255)
                                                                                                                            gCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCAtt                         >  1:1940546/1‑150 (MQ=255)
                                                                                                                             cTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTc                        <  2:969873/150‑1 (MQ=255)
                                                                                                                             cTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTc                        >  1:1977727/1‑150 (MQ=255)
                                                                                                                             cTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTc                        <  2:514435/150‑1 (MQ=255)
                                                                                                                              tgtgCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCa                       <  2:1568492/150‑1 (MQ=255)
                                                                                                                               gtgCGATTCTGGATAGCCCGAAATAGTCAACTGCAGGCTATCCAGAGAGCGGAATTATTCCGACAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACTAACATCCTGGGGGATTTTCAGCTCGTCACCCAGTGCATTCAg                      >  2:1995783/1‑150 (MQ=255)
                                                                                                                                tgCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAgg                     <  2:1873802/150‑1 (MQ=255)
                                                                                                                                tgCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAgg                     <  2:347775/150‑1 (MQ=255)
                                                                                                                                  cGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCTGTGCATTCAGGTa                   <  1:976781/150‑1 (MQ=255)
                                                                                                                                   gATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTaa                  >  2:1531387/1‑150 (MQ=255)
                                                                                                                                      tCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTg               <  2:1061955/150‑1 (MQ=255)
                                                                                                                                       cTGGATAGCCTGAAGTTGACTATTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCAGTCAGGTAACTGc              >  2:835464/1‑150 (MQ=255)
                                                                                                                                       cTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGc              <  2:2093396/150‑1 (MQ=255)
                                                                                                                                        tGGATAGCCTGAAGTTGACTATTTCGGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCg             >  2:896018/1‑150 (MQ=39)
                                                                                                                                        tGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATTCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCg             <  2:1080767/150‑1 (MQ=255)
                                                                                                                                          gATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTTCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGtt           <  1:1411428/150‑1 (MQ=255)
                                                                                                                                               cccGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATa      <  2:2085389/150‑1 (MQ=255)
                                                                                                                                                  gAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATAGCGTTCAATGCAGTCCCGAACATCATGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATaaaa   >  1:147981/1‑150 (MQ=255)
                                                                                                                                                   aaaTAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAAt  <  1:919981/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAAT  >  NC_000913/1928867‑1929163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: