Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,929,020 T→A 6.5% intergenic (‑181/+28) ptrB ← / ← yebE protease II/DUF533 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,929,0200TA 6.5% 282.4 / 7.3 102intergenic (‑181/+28)ptrB/yebEprotease II/DUF533 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (40/55);  new base A (3/4);  total (43/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 102 total aligned reads displayed.

AGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCA  >  NC_000913/1928872‑1929168
                                                                                                                                                    |                                                                                                                                                    
aGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTc                                                                                                                                                     <  2:432503/150‑1 (MQ=255)
 gggTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGTGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCa                                                                                                                                                    <  1:1345826/150‑1 (MQ=255)
     ggCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACtt                                                                                                                                                <  2:1304153/150‑1 (MQ=255)
      gCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTc                                                                                                                                               >  1:1568492/1‑150 (MQ=255)
       cAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCa                                                                                                                                              <  2:1745650/150‑1 (MQ=255)
        aaaaCTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAg                                                                                                                                             >  1:969873/1‑150 (MQ=255)
        aaaaCTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAg                                                                                                                                             <  1:581064/150‑1 (MQ=255)
               aCCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATcc                                                                                                                                      <  1:2098095/150‑1 (MQ=39)
                ccGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCa                                                                                                                                     <  1:1606012/150‑1 (MQ=39)
                ccGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCa                                                                                                                                     <  1:2098996/150‑1 (MQ=255)
                ccGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTCTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCa                                                                                                                                     <  1:1970016/150‑1 (MQ=38)
                 cGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGGCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCag                                                                                                                                    <  2:317765/150‑1 (MQ=38)
                   gggATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCagag                                                                                                                                  <  1:1907894/150‑1 (MQ=255)
                       tGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCg                                                                                                                              <  1:28394/150‑1 (MQ=255)
                       tGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCg                                                                                                                              >  2:919981/1‑150 (MQ=255)
                        gCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCCGGCTATCCAGAGAGCgg                                                                                                                             <  1:2078347/150‑1 (MQ=255)
                        gCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCgg                                                                                                                             <  2:1985523/150‑1 (MQ=255)
                        gCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCgg                                                                                                                             >  2:1411428/1‑150 (MQ=255)
                         cAAGCGAAACAGGGGAGTCATTGCTTAGATGTTGACAGGTAATGGCGCCGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTTTGCGATTCTGGATAGCCCGAAATAGTCTACTTCAGTCTAACAATAGAGCTGa                                                                                                                            >  1:323110/1‑150 (MQ=255)
                         cAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGa                                                                                                                            <  1:2078934/150‑1 (MQ=255)
                         cAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGa                                                                                                                            >  1:1394979/1‑150 (MQ=255)
                           aGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCACCTTCAGGCTATCCAGAGAGCGGAat                                                                                                                          >  1:340978/1‑150 (MQ=255)
                           aGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAat                                                                                                                          >  2:1389066/1‑150 (MQ=255)
                            gCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAatt                                                                                                                         >  1:1224033/1‑150 (MQ=255)
                              gAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATAGAATGATATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGAATCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAattat                                                                                                                       >  1:820943/1‑150 (MQ=255)
                                 aCAGGGGAGTCATTGCTTAGATGATGACATGTAATGGCTCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATGCTGGATAGCCCGAAATAGTCCACTTCAGGCTATCCAGAGAGCGGAATTATTcc                                                                                                                    <  2:916783/150‑1 (MQ=255)
                                  cAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCg                                                                                                                   <  2:1660104/150‑1 (MQ=255)
                                      ggAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCaa                                                                                                               <  1:645098/150‑1 (MQ=255)
                                            aTTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCg                                                                                                         >  1:1824457/1‑150 (MQ=255)
                                               gCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGttt                                                                                                      >  1:1722661/1‑150 (MQ=255)
                                                cTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGtttt                                                                                                     >  1:514435/1‑150 (MQ=255)
                                                cTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGtttt                                                                                                     >  1:593191/1‑150 (MQ=255)
                                                   aGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGc                                                                                                  <  2:2012897/150‑1 (MQ=255)
                                                         tgaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCg                                                                                            <  2:1225170/150‑1 (MQ=255)
                                                         tgaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCg                                                                                            <  2:953364/150‑1 (MQ=255)
                                                          gaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCga                                                                                           <  2:1212695/150‑1 (MQ=255)
                                                          gaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCga                                                                                           <  1:775853/150‑1 (MQ=255)
                                                           aCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCgag                                                                                          <  1:1981134/150‑1 (MQ=255)
                                                           aCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCgag                                                                                          <  1:1905725/150‑1 (MQ=255)
                                                            cAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCgaga                                                                                         >  2:2111380/1‑150 (MQ=255)
                                                             aGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGAt                                                                                        >  1:587707/1‑150 (MQ=255)
                                                             aGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTACCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTGGCTGTTCGAGAt                                                                                        <  1:1054728/150‑1 (MQ=255)
                                                              ggTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATc                                                                                       >  1:1080767/1‑150 (MQ=255)
                                                                    ggCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTc                                                                                 <  1:2057208/150‑1 (MQ=255)
                                                                    ggCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTc                                                                                 >  2:7838/1‑150 (MQ=255)
                                                                    ggCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTCCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTc                                                                                 <  2:1851927/150‑1 (MQ=255)
                                                                     gcgcGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCa                                                                                <  2:486178/150‑1 (MQ=255)
                                                                     gcgcGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCa                                                                                <  2:1183124/150‑1 (MQ=255)
                                                                      cgcgGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCaa                                                                               >  1:857511/1‑150 (MQ=255)
                                                                      cgcgGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAACGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCaa                                                                               >  1:1305853/1‑150 (MQ=255)
                                                                          gATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGcc                                                                           <  2:1007982/150‑1 (MQ=255)
                                                                            tatCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGt                                                                         <  2:595705/150‑1 (MQ=255)
                                                                              tCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCa                                                                       <  2:1824457/150‑1 (MQ=255)
                                                                               cGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCAc                                                                      >  2:1753814/1‑150 (MQ=255)
                                                                               cGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCAc                                                                      >  2:1501847/1‑150 (MQ=255)
                                                                                gAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACg                                                                     >  2:1143402/1‑150 (MQ=255)
                                                                                   tGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCTCGTTCAATGCCGTCACGAAc                                                                  >  2:1373294/1‑150 (MQ=255)
                                                                                      tATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATc                                                               >  2:752819/1‑150 (MQ=255)
                                                                                       aTGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGCTCGCGTTCAGTGCCGTCACGAACATcc                                                              >  2:575719/1‑150 (MQ=255)
                                                                                        tGCAGACAGAGAAAATCAGCCTGTTCTAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCt                                                             <  2:2097035/150‑1 (MQ=255)
                                                                                         gCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTg                                                            <  2:1221582/150‑1 (MQ=255)
                                                                                          cagacagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgg                                                           <  2:1378357/150‑1 (MQ=255)
                                                                                          cagacagaGAAAATCAGACTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCCTTTTTGCAGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgg                                                           >  1:375849/1‑150 (MQ=255)
                                                                                            gacagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAAATATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgggg                                                         <  1:67060/150‑1 (MQ=255)
                                                                                              cagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGa                                                       <  1:845000/150‑1 (MQ=255)
                                                                                               agagAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGACAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAt                                                      >  2:1863784/1‑150 (MQ=255)
                                                                                                gagaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAtt                                                     >  1:988315/1‑150 (MQ=255)
                                                                                                gagaAAAACAGCCTGTTCAAATGGCTGTGAGATTCTGGATAGACCGAAATAGTCAACTTCAGGCTATACAGAGAGCGGAATTATTCCTCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAtt                                                     >  2:939117/1‑150 (MQ=255)
                                                                                                 agaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAttt                                                    >  2:1274128/1‑150 (MQ=255)
                                                                                                  gaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTTCTGTTCGAGATCGCGTTCAATGCCGTCACGAAAATCCTGGGGGAtttt                                                   >  1:1766566/1‑150 (MQ=255)
                                                                                                   aaaaTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTc                                                  <  2:2091994/150‑1 (MQ=255)
                                                                                                   aaaaTCAGCCTGTACAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTACGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTc                                                  <  1:986254/150‑1 (MQ=255)
                                                                                                       tCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAgcg                                              <  1:1039955/150‑1 (MQ=255)
                                                                                                        cAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAgcgc                                             >  2:813024/1‑150 (MQ=255)
                                                                                                         aGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGcgcg                                            <  2:1879405/150‑1 (MQ=255)
                                                                                                                  aaaTGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGt                                   >  1:713017/1‑150 (MQ=255)
                                                                                                                   aaTGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTg                                  <  1:1610820/150‑1 (MQ=255)
                                                                                                                     tGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCa                                <  2:1394979/150‑1 (MQ=255)
                                                                                                                       gCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCAtt                              >  1:1940546/1‑150 (MQ=255)
                                                                                                                        cTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTc                             <  2:514435/150‑1 (MQ=255)
                                                                                                                        cTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTc                             <  2:969873/150‑1 (MQ=255)
                                                                                                                        cTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTc                             >  1:1977727/1‑150 (MQ=255)
                                                                                                                         tgtgCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCa                            <  2:1568492/150‑1 (MQ=255)
                                                                                                                          gtgCGATTCTGGATAGCCCGAAATAGTCAACTGCAGGCTATCCAGAGAGCGGAATTATTCCGACAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACTAACATCCTGGGGGATTTTCAGCTCGTCACCCAGTGCATTCAg                           >  2:1995783/1‑150 (MQ=255)
                                                                                                                           tgCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAgg                          <  2:347775/150‑1 (MQ=255)
                                                                                                                           tgCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAgg                          <  2:1873802/150‑1 (MQ=255)
                                                                                                                             cGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCTGTGCATTCAGGTa                        <  1:976781/150‑1 (MQ=255)
                                                                                                                              gATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTaa                       >  2:1531387/1‑150 (MQ=255)
                                                                                                                                  cTGGATAGCCTGAAGTTGACTATTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCAGTCAGGTAACTGc                   >  2:835464/1‑150 (MQ=255)
                                                                                                                                  cTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGc                   <  2:2093396/150‑1 (MQ=255)
                                                                                                                                   tGGATAGCCTGAAGTTGACTATTTCGGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCg                  >  2:896018/1‑150 (MQ=39)
                                                                                                                                   tGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATTCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCg                  <  2:1080767/150‑1 (MQ=255)
                                                                                                                                     gATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTTCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGtt                <  1:1411428/150‑1 (MQ=255)
                                                                                                                                          cccGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATa           <  2:2085389/150‑1 (MQ=255)
                                                                                                                                             gAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATAGCGTTCAATGCAGTCCCGAACATCATGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATaaaa        >  1:147981/1‑150 (MQ=255)
                                                                                                                                              aaaTAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAAt       <  1:919981/150‑1 (MQ=255)
                                                                                                                                                   gTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtca  >  1:359271/1‑150 (MQ=255)
                                                                                                                                                   gTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtca  <  2:1705035/150‑1 (MQ=255)
                                                                                                                                                   gTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtca  <  1:1274128/150‑1 (MQ=255)
                                                                                                                                                   gTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtca  >  1:10686/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCA  >  NC_000913/1928872‑1929168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: