Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,932,094 C→G 5.9% intergenic (+35/+21) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,932,0940CG 5.9% 402.2 / 12.9 133intergenic (+35/+21)purT/edaphosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
Reads supporting (aligned to +/- strand):  ref base C (60/65);  new base G (4/3);  total (65/68)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

Only 100 of 141 total aligned reads displayed.

GCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTA  >  NC_000913/1931945‑1932243
                                                                                                                                                     |                                                                                                                                                     
gCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATcc                                                                                                                                                       >  2:589508/1‑150 (MQ=255)
gCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATcc                                                                                                                                                       <  1:1922701/150‑1 (MQ=255)
  cGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTc                                                                                                                                                     >  1:1748491/1‑150 (MQ=255)
   ggAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCg                                                                                                                                                    >  2:1870473/1‑150 (MQ=255)
   ggAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCg                                                                                                                                                    >  1:2195829/1‑150 (MQ=255)
      aaTTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATc                                                                                                                                                 <  1:807760/150‑1 (MQ=255)
        ttGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCgg                                                                                                                                               <  2:164281/150‑1 (MQ=255)
          gATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCTGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGc                                                                                                                                             <  1:1108748/150‑1 (MQ=255)
          gATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGc                                                                                                                                             <  1:796980/150‑1 (MQ=255)
           aTGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCa                                                                                                                                            <  1:948874/150‑1 (MQ=255)
             ggCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAtt                                                                                                                                          <  2:1039341/150‑1 (MQ=255)
             ggCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtt                                                                                                                                          <  1:843270/150‑1 (MQ=255)
                 gCCGTTGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa                                                                                                                                      >  1:1613347/1‑150 (MQ=255)
                   cgtcgtCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAtttttgta                                                                                                                                    <  1:2191136/150‑5 (MQ=255)
                    gtcgtcTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGGAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                                                                                                                   >  2:103356/1‑150 (MQ=255)
                         cTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                                                                                                              >  1:1283782/1‑150 (MQ=255)
                          tGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAg                                                                                                                             >  2:767257/1‑150 (MQ=255)
                           gggggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGc                                                                                                                            >  2:1875601/1‑150 (MQ=255)
                           gggggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGc                                                                                                                            <  1:348980/150‑1 (MQ=255)
                           gggggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGc                                                                                                                            >  2:1184224/1‑150 (MQ=255)
                           gggggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGc                                                                                                                            <  1:1133114/150‑1 (MQ=255)
                             gggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCtt                                                                                                                          >  2:543624/1‑150 (MQ=255)
                                  gCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcgc                                                                                                                     >  2:1162271/1‑150 (MQ=255)
                                   cACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTACAGCTTAGCGCCt                                                                                                                   <  2:1511896/150‑1 (MQ=255)
                                    aCTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCt                                                                                                                   >  2:1910910/1‑150 (MQ=255)
                                      tGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCAACAAAAATGCCCGATCCTCGATCGGGCAGTTTGCCTTTTACAGCTTCGCGccatg                                                                                                                 >  1:264668/1‑147 (MQ=255)
                                       ggCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAATATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCt                                                                                                                <  2:458358/150‑1 (MQ=255)
                                       ggCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAACTACATGATTAAACCCGGCATAAAAACGATAAAAAAAACCCCCATCCTCGTACGGGCATTTTGACTTTTACAGCTTAGCGGCTTCt                                                                                                                >  2:140150/1‑150 (MQ=255)
                                        gCTACTGCAGAGAGCGTTCTAGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACTCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTa                                                                                                               <  1:650364/150‑1 (MQ=255)
                                          tACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCAGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACa                                                                                                             <  1:476286/150‑1 (MQ=255)
                                           aCTTCAGAGATTCTAGATCACGCCATTTAACGCGCGAAGCACTCCGCCGGACATGTTAAAGTACATGTTTAAACCCTGCAATAAAACGCTACAAAAATTCCCGATCCTCGATCGTGCATTTTGACTTATACAGCTTAGCGCCTTCTACAg                                                                                                            <  1:299088/150‑1 (MQ=255)
                                            cTGCAGAGAGTGTTGTTGACGCGATTGACCGCGCGAAGCACGCCGGCTGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGc                                                                                                           <  2:955727/150‑1 (MQ=255)
                                            cTGCAGAGAGTGTTGTTGACGCAATTGAACGCGCGAAGCACGACGCCGGACAGGTAAAAGTACAGGGTTTAACGCGGCAAAACAACGCTACAAAAATCCCCGAGCCTGGATCGGGGATGTTGACTTTTACAGCTTCGCGCGTTCTACAGc                                                                                                           >  2:1209434/1‑150 (MQ=255)
                                              gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAATACGCTACAAAAATGCCCGATCCTCGATCGGGGATTTTGACTTTTACTGCTTAGCGCCTTCTACAGCtt                                                                                                         >  1:1783298/1‑150 (MQ=255)
                                              gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGATTAGCGCCTTCTACAGCtt                                                                                                         >  1:1756492/1‑150 (MQ=255)
                                                 gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAACCGCTACAAAAATGCCCGATCCTAGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAc                                                                                                      >  2:1868127/1‑150 (MQ=255)
                                                 gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAc                                                                                                      >  2:635126/1‑150 (MQ=255)
                                                      tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGcc                                                                                                 <  1:18536/150‑1 (MQ=255)
                                                      ggttgttGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGcc                                                                                                 <  1:1091581/149‑1 (MQ=255)
                                                      ggttgttGACGCCATTGAAAGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGcc                                                                                                 <  1:1543018/149‑1 (MQ=255)
                                                         tgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGc                                                                                              <  2:723452/150‑1 (MQ=255)
                                                          gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCt                                                                                             >  2:1241287/1‑150 (MQ=255)
                                                           ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCtt                                                                                            <  1:518947/150‑1 (MQ=255)
                                                            tgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTa                                                                                           >  2:1254568/1‑150 (MQ=255)
                                                             gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAg                                                                                          <  1:589508/150‑1 (MQ=255)
                                                             gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAg                                                                                          >  2:1686454/1‑150 (MQ=255)
                                                             gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGACATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAg                                                                                          <  2:1682632/150‑1 (MQ=255)
                                                                  cATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATg                                                                                     >  2:660904/1‑150 (MQ=255)
                                                                  cATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATg                                                                                     >  2:904782/1‑150 (MQ=255)
                                                                        aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                                                                               <  1:1794331/150‑1 (MQ=255)
                                                                        aCGCGCGAAGCACGCCGCCGGACACGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                                                                               <  2:226472/150‑1 (MQ=255)
                                                                         cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                              >  2:2026763/1‑150 (MQ=255)
                                                                           cgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTaa                                                                            >  2:1374764/1‑150 (MQ=255)
                                                                             cgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATc                                                                          <  2:1578614/150‑1 (MQ=255)
                                                                                 gCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGccc                                                                      <  1:385029/150‑1 (MQ=255)
                                                                                 ccACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGccc                                                                      <  1:546349/149‑1 (MQ=255)
                                                                                    cgccgcCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCt                                                                   >  2:1247337/1‑150 (MQ=255)
                                                                                       cgccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTcc                                                                <  1:240718/150‑1 (MQ=255)
                                                                                         ccgGACAGGTAAAAGTACAGGGTTAAACCCGTCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAg                                                              <  1:441713/150‑1 (MQ=255)
                                                                                           ggACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCGgcg                                                            >  1:1028245/1‑150 (MQ=255)
                                                                                                ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCAt                                                         >  1:900823/1‑148 (MQ=255)
                                                                                                ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCAt                                                         <  2:900823/148‑1 (MQ=255)
                                                                                                ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCt                                                       >  2:1029130/1‑150 (MQ=255)
                                                                                                   aaaaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCTTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCCGCGCATCTGcc                                                    >  2:852246/1‑150 (MQ=255)
                                                                                                   aaaaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGcc                                                    <  1:2156195/150‑1 (MQ=255)
                                                                                                    aaaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCg                                                   <  2:1918188/150‑1 (MQ=255)
                                                                                                     aaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCgg                                                  <  2:798602/150‑1 (MQ=255)
                                                                                                     aaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACACCTTAGCTCCTTCTACAGCTTCACGCGCCTGCATAGTAATGCGGTCGTACTCGCCCGCTTCCAGCGCATCTGCCgg                                                  >  2:1358831/1‑150 (MQ=255)
                                                                                                     aaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGAGCCTCGCTCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGAGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCgg                                                  >  2:1351680/1‑150 (MQ=255)
                                                                                                            gggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagc                                           <  1:667253/150‑1 (MQ=255)
                                                                                                             ggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcc                                          >  2:1970034/1‑150 (MQ=255)
                                                                                                             ggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGGCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcc                                          <  1:1184224/150‑1 (MQ=255)
                                                                                                              gTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCCGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGACccagcca                                         >  1:421803/1‑150 (MQ=255)
                                                                                                                  aaCCCGGCAAAAAAACGCTACAAAAATGCCGGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGaa                                     <  2:229249/150‑1 (MQ=255)
                                                                                                                   aCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGACCCAGCCAGGAac                                    >  1:1896658/1‑150 (MQ=255)
                                                                                                                     ccGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAacca                                  <  1:1479428/150‑1 (MQ=255)
                                                                                                                       ggCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAaccacc                                <  2:262500/150‑1 (MQ=255)
                                                                                                                        gCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCg                               >  2:973854/1‑150 (MQ=255)
                                                                                                                         cAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACCGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGa                              >  1:296089/1‑150 (MQ=255)
                                                                                                                            aaaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGc                           <  1:1523327/150‑1 (MQ=255)
                                                                                                                               aaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCGGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGcaca                        <  2:1028245/150‑1 (MQ=255)
                                                                                                                               aaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGcaca                        <  1:5185/150‑1 (MQ=255)
                                                                                                                                 cGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGCACCACCGATGCACAGc                      >  1:1835682/1‑150 (MQ=255)
                                                                                                                                   caaagacaaTACCCGCTGCTCGATCGGGCCTTTTTACTTTTACAGATTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCAc                    <  2:438203/143‑1 (MQ=255)
                                                                                                                                      tcaaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCCGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAGCAGCCAGGAACCACCGATGCCCAGCACGCt                 >  1:1484557/3‑150 (MQ=255)
                                                                                                                                      cAAAAATGCCCGATCCTCGATCGGCCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGCTCGTAATCGCCCGCTTCCAGCGCAACTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGcg                 >  2:1402697/1‑149 (MQ=255)
                                                                                                                                       aaaaaTGCCCGATCCTCGATAGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATATGCCGGAACCTGCCAGGAACCACCGATGCACAGCACGCtt                <  2:1065974/150‑1 (MQ=255)
                                                                                                                                        aaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCttt               >  1:1353828/1‑150 (MQ=255)
                                                                                                                                        aaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCttt               <  1:673901/150‑1 (MQ=255)
                                                                                                                                        aaaaTGCCCGATCCTCGATCGGGAATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCttt               <  1:1991031/150‑1 (MQ=255)
                                                                                                                                          aaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCAACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTc             >  1:1037/1‑150 (MQ=255)
                                                                                                                                           aTGCCCGATCGAGGATCGGGCATTTTGCCTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGATTAGTAATGCGTTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCAACGATGCACAGCCCGCtattaa            >  1:167704/1‑148 (MQ=255)
                                                                                                                                           aTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCa            <  1:246730/150‑1 (MQ=255)
                                                                                                                                           aTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCa            <  2:1633932/150‑1 (MQ=255)
                                                                                                                                             gCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAgc          >  2:1130248/1‑150 (MQ=255)
                                                                                                                                                 gATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGAGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCa      >  1:1058238/1‑150 (MQ=255)
                                                                                                                                                  aTCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAg     <  1:1373245/150‑1 (MQ=255)
                                                                                                                                                     cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTa  >  2:1516798/1‑150 (MQ=255)
                                                                                                                                                     cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTa  <  1:1891454/150‑1 (MQ=255)
                                                                                                                                                     cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCGTTTCAGCGCCAGGTa  >  1:207133/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTA  >  NC_000913/1931945‑1932243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: