Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,993,949 T→G 9.1% D253A (GAC→GCC)  uvrC ← excinuclease UvrABC, endonuclease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,993,9490TG 9.1% 264.7 / 4.8 102D253A (GAC→GCC) uvrCexcinuclease UvrABC, endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base T (42/48);  new base G (4/7);  total (47/55)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TGGCCTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCA  >  NC_000913/1993802‑1994098
                                                                                                                                                   |                                                                                                                                                     
tGGCCTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCg                                                                                                                                                     <  1:2193555/150‑1 (MQ=255)
 ggCCTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGa                                                                                                                                                    <  2:1681866/150‑1 (MQ=255)
  gCCTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAg                                                                                                                                                   <  1:1639799/150‑1 (MQ=255)
  gCCTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAg                                                                                                                                                   <  2:450746/150‑1 (MQ=255)
   ccTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCTCATCGAACGCCACACCAATAACGTCGAgg                                                                                                                                                  <  2:1005440/150‑1 (MQ=255)
   ccTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAgg                                                                                                                                                  >  1:733433/1‑150 (MQ=255)
    cTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTAACGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGCACAATACGTGTACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGgt                                                                                                                                                 >  1:1929880/1‑150 (MQ=255)
    cTACGAAGGGTTCTACCACATCGCTCAGGTCCGTTCCGCCAGCCACTTTCGCGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACTAATGAACAATACGTGGACACAAGCCATGCCCGCATCGATCGCTACACCTATAACGGCGAGgt                                                                                                                                                 <  2:203562/150‑1 (MQ=255)
     tACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGCGCAGTTTGCCCTGACGAATGACCAATACGTGGACACAAGCCATGCCCTCATCGAAAGCCACACGAATAACGGCGAGgtc                                                                                                                                                >  2:138473/1‑150 (MQ=255)
         aaGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCCCTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGACCGCCACACGAATAACGGCGAGGtcgtcg                                                                                                                                            >  2:112204/1‑150 (MQ=255)
             tttCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCgg                                                                                                                                        <  1:1628731/150‑1 (MQ=255)
              ttCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGt                                                                                                                                       <  2:1725375/150‑1 (MQ=255)
               tCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGGATCGAACGCCCCACCAATAACGTCGCGGGCGTCGCCgggt                                                                                                                                      >  2:1190437/1‑148 (MQ=255)
               tCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTa                                                                                                                                      >  1:2080461/1‑150 (MQ=255)
                 tACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTAtt                                                                                                                                    >  1:529071/1‑150 (MQ=255)
                   ccaccTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTgg                                                                                                                                  >  2:1866199/1‑150 (MQ=255)
                   ccaccTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTgg                                                                                                                                  <  2:1469498/150‑1 (MQ=255)
                   ccaccTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTgg                                                                                                                                  <  2:1544168/150‑1 (MQ=255)
                   ccaccTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTgg                                                                                                                                  <  2:602753/150‑1 (MQ=255)
                    caccTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGa                                                                                                                                 <  2:548581/150‑1 (MQ=255)
                     cccTCGCTCCCTTCCGCCCCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTGGCCCTGACGAATTAACAATATGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATACCGGCGACGACCTCGACGTTATTggtg                                                                                                                                <  2:1068376/149‑3 (MQ=255)
                     accTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATCGGaa                                                                                                                                <  1:2066221/150‑1 (MQ=255)
                               ggtCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGTGCCCCACCCCCATAACGGCGAGGTCGTCGCCGGGATTGGAATCGAAGTGtt                                                                                                                      >  1:1301264/3‑150 (MQ=255)
                                ttCCGTACCGCCAGGCAATTTCGGGAATTAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAAAACGTGGACACAAGCCAGGCCCGCATCGAACGCCACACCAATAACGTCGAGGTAGTCGCCGGTATTGGAAACGAATGGtat                                                                                                                     >  2:1117189/1‑148 (MQ=255)
                                  ccGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGttttt                                                                                                                   >  2:1889912/1‑150 (MQ=255)
                                   cGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTc                                                                                                                  <  2:1598445/150‑1 (MQ=255)
                                    ggaCCGCCAGGCAATTTCGGGAAATAGCTGCGGCTGCAGAGCACTTTGCACTGACGAATGAACAATACTTGCACACAATACATGCACGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCg                                                                                                                 <  2:372563/148‑1 (MQ=255)
                                    gTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCg                                                                                                                 >  2:1738089/1‑150 (MQ=255)
                                    gTACCGCCAGGCACTTTCCGGTAATAGCTGCGGCGGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCg                                                                                                                 <  1:74342/150‑1 (MQ=255)
                                     tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCgg                                                                                                                <  1:2197944/150‑1 (MQ=255)
                                     tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCgg                                                                                                                <  1:1132654/150‑1 (MQ=255)
                                     tACCGCCAGGCACTTTCGGGAAATACCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTTGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCgg                                                                                                                <  2:94358/150‑1 (MQ=255)
                                       ccGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGCACGAAACGTGGACACAAGCCATGCCCGCCTCGAACGCCACACCAATAACGTCGAGGTCCTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                                              >  1:388158/1‑150 (MQ=255)
                                       ccGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                                              >  2:2050800/1‑150 (MQ=255)
                                       ccGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                                              <  1:499185/150‑1 (MQ=255)
                                       ccGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                                              <  2:473792/150‑1 (MQ=255)
                                         cccAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAAAGAACAATACGTGGACACAACCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTGTTTCGGTGAc                                                                                                            >  1:1024841/2‑150 (MQ=255)
                                          cgaGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGAcg                                                                                                           <  2:246391/148‑1 (MQ=255)
                                          ccAGGCACTTTCGGGAAATGGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGAcg                                                                                                           <  2:1234278/150‑1 (MQ=255)
                                            aGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGTATGAACATTACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGAcgcg                                                                                                         <  1:1988091/150‑1 (MQ=255)
                                              gCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGCCGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGACCGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGATTTTCGGTGACGCGTc                                                                                                       >  1:338052/1‑150 (MQ=255)
                                              gCACTTTCGGGAAATAGCGGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTc                                                                                                       <  1:573855/150‑1 (MQ=255)
                                               cACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCg                                                                                                      >  2:2208978/1‑150 (MQ=255)
                                               cACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCg                                                                                                      <  2:644931/150‑1 (MQ=255)
                                                aCTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGc                                                                                                     <  2:962140/150‑1 (MQ=255)
                                                 cTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                                    <  1:2050800/150‑1 (MQ=255)
                                                    tCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCCCCg                                                                                                 >  2:2214669/1‑150 (MQ=255)
                                                        gAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTg                                                                                             <  2:106106/150‑1 (MQ=255)
                                                              gCTGCGGCTGCCGAGCACTTTGCCCTGCCGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGCACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAAtttt                                                                                       >  2:278602/1‑149 (MQ=255)
                                                               cTGCGGCTGCCGAGCACGTTGCCCTGACGAATGAACAATACGTGGACACTAGGCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTgg                                                                                      <  2:1205415/150‑1 (MQ=255)
                                                                tGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCCTGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGt                                                                                     >  1:1037194/1‑150 (MQ=255)
                                                                 gCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTc                                                                                    >  1:2069793/1‑150 (MQ=255)
                                                                    gCTGCCGAGCACTTTGCCCTGACGAATTAAAAATACGTGGACACAAGCCATGCCAGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGACGGTATTGGAATCGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTcgcg                                                                                 <  1:2050696/150‑1 (MQ=255)
                                                                       gCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAAt                                                                              >  2:602031/1‑150 (MQ=255)
                                                                        ccGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATa                                                                             <  1:1210500/150‑1 (MQ=255)
                                                                        ccGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGACGAGGTCGTCGCCGGTATTGGCATCGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATa                                                                             >  1:637349/1‑150 (MQ=255)
                                                                         cGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGaa                                                                               <  1:1352918/147‑1 (MQ=255)
                                                                         cGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGaa                                                                               >  2:1352918/1‑147 (MQ=255)
                                                                                   gCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTAGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGattc                                                                  >  1:223213/1‑150 (MQ=255)
                                                                                    cccTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGcttct                                                                 >  1:1992141/1‑150 (MQ=255)
                                                                                      cTGACGAATGAACAATACGTGGCCACCAGCCATGACCGCATCGACCGCCACACCAATACCGTCGAGGGCGTCGCCGGTATAGGAAACGTTTTGTTTTTCGGGGCCGCGTCGCCACGCCTGTATTAGGTCGCGAAGACGTGCTGcgtctgc                                                               >  2:1534470/1‑148 (MQ=255)
                                                                                      cTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCttcttc                                                               >  1:564195/1‑150 (MQ=255)
                                                                                             aTGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTcc                                                        <  2:651021/150‑1 (MQ=255)
                                                                                             aTGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTcc                                                        >  1:1350211/1‑150 (MQ=255)
                                                                                                aaCAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGa                                                     <  1:2182017/150‑1 (MQ=255)
                                                                                                  cAATACGTGGACACAAGCCATGCCCTCATCGAACGCCACACAAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGACTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGAtt                                                   >  1:118317/1‑150 (MQ=255)
                                                                                                  cAATACGTGGACACAAGCCATGCCAGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCTTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCGTCCAACTCCAGAtg                                                   >  2:1083421/1‑149 (MQ=255)
                                                                                                     tACGTGGACACAAGCCATGCCCGCATCGAACGACTCACCAATAACGGCGTCGTCGTCGCCGGTATTGGAAACGAATAGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATTCGTGCAGCTTCTTCATACTCCAGATTctg                                                <  2:899730/150‑1 (MQ=255)
                                                                                                      aCGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctgg                                               <  1:1319946/150‑1 (MQ=255)
                                                                                                        gTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggct                                             >  2:384732/1‑150 (MQ=255)
                                                                                                         tGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGGCGAGGGCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggctg                                            <  1:1553948/150‑1 (MQ=255)
                                                                                                           aacacCAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAAGTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGGGCAGCTTCGTCCAACTCCAGATTCtggctggc                                          >  2:1232918/2‑150 (MQ=255)
                                                                                                            acacAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCa                                         >  1:2118688/1‑150 (MQ=255)
                                                                                                               caAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGtt                                      >  2:879576/1‑150 (MQ=255)
                                                                                                                  gCCCTGCCCGCATCGAACGCCACACCAATAACGGCGAGGTCGTCGCAGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                                   <  2:2030042/150‑1 (MQ=255)
                                                                                                                  gCCATGCGCGCATCGAACGCCACAGCAATATCGTCGAGGTCGTCGCCGGTATTGGAAACGATTTGTTTTTCGGTGACGCGTCGCACCGCATGAATTTGGTCGCGAATAAGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                                   <  2:1024841/150‑1 (MQ=255)
                                                                                                                  gCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                                   >  2:1363406/1‑150 (MQ=255)
                                                                                                                  gCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                                   <  2:733433/150‑1 (MQ=255)
                                                                                                                  gCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGGGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                                   <  2:2021746/150‑1 (MQ=255)
                                                                                                                   ccATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCa                                  <  2:564195/150‑1 (MQ=255)
                                                                                                                    cATGCCCGCATCGAACGCCACACAAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAACCTCCAGCTTCTGGCTGGCAGTTTCCAt                                 >  2:1407289/1‑150 (MQ=255)
                                                                                                                     aTGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATa                                <  1:188702/150‑1 (MQ=255)
                                                                                                                      tGCCCGCATCGAACGCCACTCCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATAc                               <  1:2136041/150‑1 (MQ=255)
                                                                                                                        cccGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGa                             >  2:55579/1‑150 (MQ=255)
                                                                                                                         ccGCATCGACCGCCACACCAATAACGGCGAGGTCGTCGCCGGAATTGGAAACGAAGTGTTTTTCGGTGACGCGTCGCACCGCCTGAAGTTGGTCGCGAAGACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGAc                            <  1:138473/150‑1 (MQ=255)
                                                                                                                         ccGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGAc                            <  1:741541/150‑1 (MQ=255)
                                                                                                                         ccGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCGGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTACATTCGAc                            >  1:1534496/1‑150 (MQ=255)
                                                                                                                               cGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGa                      >  2:380820/1‑150 (MQ=255)
                                                                                                                                  aCGCCACACCACTATCGTCCAGGTCGTCGCCGGTATTGGAAACTAATGGTTTTTCGGGGACGCGTCGCACCGCCTGAATTTGGTCGCGACTACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTCGCAGTTTCCATACGACTAATGAGtt                   <  2:1525437/150‑1 (MQ=255)
                                                                                                                                  aCGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGtt                   <  1:1270473/150‑1 (MQ=255)
                                                                                                                                   cGCCACACCAATAACGGCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTg                  <  1:57039/150‑1 (MQ=255)
                                                                                                                                      gtttccAATACCGGCGACGACCTCGACGTTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATTCGACTAATGAGTTGCGt               >  1:1430003/5‑150 (MQ=255)
                                                                                                                                          ccAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGc           <  1:2141839/150‑1 (MQ=255)
                                                                                                                                          ccAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGCCAGTTTCCATACGACTAATGAGTAGCGTAAGc           >  2:1844346/1‑150 (MQ=255)
                                                                                                                                           cAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCa          >  1:1798482/1‑150 (MQ=255)
                                                                                                                                            aaTAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCAc         >  2:1988731/1‑150 (MQ=255)
                                                                                                                                             aTAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCAcc        >  2:1344591/1‑150 (MQ=255)
                                                                                                                                               accGTCGAGGTCGTCGCCGGTATTGGAAACGACTTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGAGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTg      <  2:2107405/148‑1 (MQ=255)
                                                                                                                                                aCGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACAGCATGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTATGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGa     >  1:1568452/1‑150 (MQ=255)
                                                                                                                                                   tCGAGGTCGTCGCCGGTATTGGAACCGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATATGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGatca  <  2:1486005/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGGCCTACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCA  >  NC_000913/1993802‑1994098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: