Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 95,071 T→C 5.2% V141A (GTA→GCA)  murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091395,0710TC 5.2% 536.6 / 12.9 173V141A (GTA→GCA) murFUDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (85/78);  new base C (3/6);  total (89/84)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.76e-01

Only 100 of 178 total aligned reads displayed.

TGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGA  >  NC_000913/94922‑95218
                                                                                                                                                     |                                                                                                                                                   
tGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGgtgt                                                                                                                                                     <  2:1262510/150‑1 (MQ=255)
 gCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGATCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATGTTACGCAAGTTCGGCAACACGCTTTATACGGCAGGCAATCTCAAAAACGACATCGGTGTa                                                                                                                                                    >  1:142156/1‑150 (MQ=255)
  cATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTAc                                                                                                                                                   >  1:719040/1‑150 (MQ=255)
   ttGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAAGACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTAcc                                                                                                                                                  <  1:117262/149‑1 (MQ=255)
   aTGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTAcc                                                                                                                                                  <  1:1119519/150‑1 (MQ=255)
     gggTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGa                                                                                                                                                >  1:1969828/1‑150 (MQ=255)
     gggTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGa                                                                                                                                                >  1:14604/1‑150 (MQ=255)
       gTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCCGGCAATCTCAACAACGACATCGGTGTACCGATg                                                                                                                                              >  1:105223/1‑150 (MQ=255)
            ccATCAAGTTCCGGCGCGCGTGGTGGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTTCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCt                                                                                                                                         <  2:1936320/150‑1 (MQ=255)
            ccAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGCTGACGGCGGCGATTTTAAGCCAGTGCGGCAACCCGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCt                                                                                                                                         >  1:1546209/1‑150 (MQ=255)
             cAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTACACCGATGTCGTTg                                                                                                                                        <  2:421402/150‑1 (MQ=255)
              aGCAAGTTCCGGCGCGCGTAGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGCCATCGGGGTACCGATGACGgtgt                                                                                                                                       >  1:187180/1‑150 (MQ=255)
               gCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGTCATCGGTACACCGATGTCgttgtt                                                                                                                                      <  1:1720407/150‑1 (MQ=255)
                cAAGTTCCGGCGCGCGTGGGTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTACACCGATGTCGttgttg                                                                                                                                     <  2:1762981/150‑1 (MQ=255)
                cAAGATCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGTCATCGGTACACCGATGTCGttgttg                                                                                                                                     <  1:1582936/150‑1 (MQ=255)
                  aGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTgcg                                                                                                                                   >  1:1059937/1‑150 (MQ=255)
                   gTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAGCGTCATCGGTACACCGATGTCGttgttgaga                                                                                                                                  <  1:2162309/150‑4 (MQ=39)
                    ttCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCt                                                                                                                                 >  1:2097239/1‑150 (MQ=255)
                    ttCCGGCGCGCGGGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCt                                                                                                                                 <  2:520682/150‑1 (MQ=255)
                           gcgcgTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGcc                                                                                                                          <  2:1076062/150‑1 (MQ=255)
                             gcgTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCgg                                                                                                                        <  1:1913337/150‑1 (MQ=255)
                                tGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAAt                                                                                                                     >  1:2004307/1‑150 (MQ=255)
                                     gCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGAAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGAt                                                                                                                >  2:117933/1‑150 (MQ=255)
                                      ctctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGAtt                                                                                                               <  1:1822609/150‑1 (MQ=255)
                                       tctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCACCACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTa                                                                                                              >  1:1141890/1‑150 (MQ=255)
                                       tctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTa                                                                                                              <  1:578732/150‑1 (MQ=255)
                                       tctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTa                                                                                                              <  2:1636689/150‑1 (MQ=255)
                                        ctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTAc                                                                                                             >  2:1111118/1‑150 (MQ=255)
                                         tGACGGGGTCCTCCGGCAAAACCTCAGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGATGTTGCGCTTAACGCAGGAATACGATTACg                                                                                                            >  2:1437513/1‑150 (MQ=255)
                                              gggTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGtt                                                                                                       >  2:595606/1‑150 (MQ=255)
                                                  cctccGGCAAATCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGATTACGATTACGCAGTTATTg                                                                                                   <  1:122217/150‑1 (MQ=255)
                                                  cctccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTg                                                                                                   <  1:2207315/150‑1 (MQ=255)
                                                     ccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAAc                                                                                                <  2:1644049/150‑1 (MQ=255)
                                                       ggCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACtt                                                                                              >  2:2119808/1‑150 (MQ=255)
                                                       ggCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACtt                                                                                              >  1:774638/1‑150 (MQ=255)
                                                       ggCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACtt                                                                                              <  1:1292850/150‑1 (MQ=255)
                                                        gCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTg                                                                                             >  1:1493652/1‑150 (MQ=255)
                                                          aaaaCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGgc                                                                                           <  2:648604/150‑1 (MQ=255)
                                                                tCCGTTAAAGAGATGACGGCGGCGATTTTAAGCGAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACATCGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGGTATTGAACTTGGCGCGAAc                                                                                     <  1:45600/150‑1 (MQ=255)
                                                                tCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAAc                                                                                     <  1:942749/150‑1 (MQ=255)
                                                                  cGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCCAACCa                                                                                   >  1:595716/1‑150 (MQ=255)
                                                                    ttAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATc                                                                                 >  1:1515179/1‑150 (MQ=255)
                                                                     tAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATcc                                                                                >  1:319369/1‑149 (MQ=255)
                                                                      aaaGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAg                                                                               >  2:1813219/1‑150 (MQ=255)
                                                                       aaGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAgg                                                                              >  1:90423/1‑150 (MQ=255)
                                                                         gagaTGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCTCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAgggg                                                                            >  1:2157026/1‑149 (MQ=255)
                                                                             tGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGaaa                                                                        >  1:1325894/1‑150 (MQ=255)
                                                                             tGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGaaa                                                                        <  2:1223901/150‑1 (MQ=255)
                                                                                  gcggcgATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGcc                                                                   <  1:690896/150‑1 (MQ=255)
                                                                                    ggcgATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTg                                                                 >  1:1190809/1‑150 (MQ=255)
                                                                                     gcgATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTgg                                                                <  1:1769217/150‑1 (MQ=255)
                                                                                         ttttAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGACCCATCAGGGCGAAATAGCCTGGACtg                                                            >  1:1792343/1‑150 (MQ=255)
                                                                                         ttttAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACtg                                                            >  2:1908721/1‑150 (MQ=255)
                                                                                             aaGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAg                                                        <  2:1476687/150‑1 (MQ=255)
                                                                                              aGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCTGTGTACCGATGACGCTGTTGCGCTTAACGCCCGAATACCATTACGCAGTTATTGAACTTCGCGCCAACCATCAGGGCGAAAAAGCCTGGACACTGAGt                                                       >  1:303045/1‑150 (MQ=255)
                                                                                              aGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGt                                                       <  1:1093705/150‑1 (MQ=255)
                                                                                               gCCAGTGCGGCAACACGCTTTATACGGCAGGCACGCTCAACAACGACATCGGTGTACCGATGACGCGGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGACCCATCAGGGCGAAATAGCCTGGACTGTGAGTc                                                      <  1:2081043/150‑1 (MQ=255)
                                                                                               gCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTc                                                      <  2:1493652/150‑1 (MQ=255)
                                                                                                ccAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCt                                                     <  2:1579397/150‑1 (MQ=255)
                                                                                                ccAGTGAGGCAACAGGCGTTATAGGGCAGGAAATCTCAACAAGGACATCGGTGTACCGATGAAGCTTTTGCGCTTAACGCCGGAATACGATTAGGCAGTTATTGAACATGGCGCGAACCATCAGGGAGAAATAGCCTGGACTGTGAGTCt                                                     <  2:75002/150‑1 (MQ=255)
                                                                                                 cAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTg                                                    >  1:976182/1‑150 (MQ=255)
                                                                                                   gTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCTGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGAc                                                  <  2:221352/150‑1 (MQ=255)
                                                                                                    tGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGAca                                                 <  2:1515179/150‑2 (MQ=255)
                                                                                                    ggCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCAGCAGGGCGAAATAGCCTGGACTGTGAGTCTGACt                                                 <  2:927427/149‑1 (MQ=255)
                                                                                                     gCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTc                                                >  2:2051353/1‑150 (MQ=255)
                                                                                                       ggCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGc                                              >  1:1137367/1‑150 (MQ=255)
                                                                                                       ggCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACAGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGAATCGc                                              >  1:1140416/1‑150 (MQ=255)
                                                                                                        gCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGcc                                             >  2:285247/1‑150 (MQ=255)
                                                                                                        gCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATAACGCAGTTATTGAACTTGGCGCGAACCCTCAGGGCGAAATAGCCAGGACTGTGAGTCTGACTCGcc                                             >  1:375513/1‑150 (MQ=255)
                                                                                                         caacaaGCTTTATACTTCAGGCAATCTAAACAACGACATCGGGGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACTCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGGGAGGCTGACTCGccc                                            <  1:1672656/150‑1 (MQ=255)
                                                                                                          aaCACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCg                                           >  1:1332168/1‑150 (MQ=255)
                                                                                                           acacGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGATTAACTCCCGAATAAAAATACTAACTAAATGAACTAGGCGAAAAAAAAAAAAAATAAATATCCTGGACTGTGAGTCTGACTCGCCCgg                                          >  2:487341/1‑150 (MQ=255)
                                                                                                                 tttATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTg                                    <  1:788485/150‑1 (MQ=255)
                                                                                                                  ttATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTgc                                   <  2:1319584/150‑1 (MQ=255)
                                                                                                                   tataCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGTCGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTgcg                                  >  2:1062125/1‑150 (MQ=255)
                                                                                                                   tataCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTAGCGCTTAACGCCGGAATACGCGTACGCAGTTATTGAACTTGGCGCGAGCCATCAGCGCGAACTCTCATGGAGTGTGAGTCTGACTCGCCCGGAAGCTgcg                                  >  1:331308/1‑150 (MQ=255)
                                                                                                                     taCGGCATGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCTCGTTACGCCGGAATACGATTACTCAGTTATTGAACTTGGCTCTAACCATCAGGGCGAAATATCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCt                                >  2:2070472/1‑150 (MQ=255)
                                                                                                                     taCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCt                                >  2:1485842/1‑150 (MQ=255)
                                                                                                                      aCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTg                               >  1:831018/1‑150 (MQ=255)
                                                                                                                       cGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTgg                              >  2:276669/1‑150 (MQ=255)
                                                                                                                         gcaggcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTc                            <  2:2097239/150‑1 (MQ=255)
                                                                                                                         gcaggcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTc                            <  2:717251/150‑1 (MQ=255)
                                                                                                                         gcaggcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTc                            >  1:567093/1‑150 (MQ=255)
                                                                                                                         gcaggcaATCTCAACAACGACATCGGGGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTc                            <  2:794334/150‑1 (MQ=255)
                                                                                                                          caggcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGGGAGTCTGACTCGCCCGGAAGCTGCGCTGGTca                           <  2:187180/150‑1 (MQ=255)
                                                                                                                            ggcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTcaac                         >  1:504499/1‑150 (MQ=255)
                                                                                                                                agcgcaacagCGTCATCGGTACACCGATGTCGTTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAAcc                     >  2:1369536/5‑150 (MQ=38)
                                                                                                                                aTCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAAcc                     <  1:1247478/150‑1 (MQ=255)
                                                                                                                                    caacagCGTCATCGGTACACCGATGTCGTTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGc                 >  2:823619/1‑150 (MQ=255)
                                                                                                                                    caacaaCGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGc                 >  2:2060537/1‑150 (MQ=255)
                                                                                                                                      acaacGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAg               <  2:1666924/150‑1 (MQ=255)
                                                                                                                                       caacGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAgc              >  1:1633562/1‑150 (MQ=255)
                                                                                                                                       caacGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAgc              >  1:100300/1‑150 (MQ=255)
                                                                                                                                           gACATCGGTGTACCGATGACTCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGAGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAgcggcg          <  2:142156/150‑1 (MQ=255)
                                                                                                                                           gACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAgcggcg          <  2:684780/150‑1 (MQ=255)
                                                                                                                                                cGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGGCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCt     <  2:1400658/150‑1 (MQ=255)
                                                                                                                                                 ggTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTg    <  1:1954789/150‑1 (MQ=255)
                                                                                                                                                 ggTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTg    >  2:878284/1‑150 (MQ=255)
                                                                                                                                                 ggTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTAAGGAGTTATTGAACTTGGCGCGAAGCATCAGGGGGAAATAGCCTGGACTGTGTGTCTGACTCGCCCGGAAGCTGCGCGGGTCTAGAACCTGTCTGCGGCGCATCTg    >  1:1384632/1‑150 (MQ=255)
                                                                                                                                                   tgtACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGa  >  1:1646559/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGA  >  NC_000913/94922‑95218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: