Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,057,601 C→A 8.8% intergenic (+27/+426) yeeN → / ← asnW UPF0082 family protein/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,057,6010CA 8.8% 335.1 / 15.7 126intergenic (+27/+426)yeeN/asnWUPF0082 family protein/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base C (63/51);  new base A (6/6);  total (69/57)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 129 total aligned reads displayed.

ATGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTAC  >  NC_000913/2057452‑2057750
                                                                                                                                                     |                                                                                                                                                     
aTGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTAc                                                                                                                                                       <  1:1953831/150‑1 (MQ=255)
aTGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTAc                                                                                                                                                       <  1:1395821/150‑1 (MQ=255)
  gATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACgg                                                                                                                                                     <  2:523472/150‑1 (MQ=255)
  gATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACgg                                                                                                                                                     <  2:1569448/150‑1 (MQ=255)
      gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTTTCTTTACGCCAGa                                                                                                                                                 >  2:379639/1‑150 (MQ=255)
      gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGa                                                                                                                                                 >  2:1573164/1‑150 (MQ=255)
         cAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAATGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAttt                                                                                                                                              >  1:48777/1‑150 (MQ=255)
            tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCtt                                                                                                                                           <  1:2129669/150‑1 (MQ=255)
            tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCtt                                                                                                                                           <  2:1957026/150‑1 (MQ=255)
             cagAAGTTGCGCGTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCATGTTGATGCCCTAGAAGATGAAGACGATGTACAAAAAGTTTATGATAACGTCGCAAATATCTAATTATCTTTTAAAGAAATCTGTCTTTACGTCAGATTTCatt                                                                                                                                          >  1:351107/3‑150 (MQ=255)
             cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTTTCTTTACGGAAGATTTCttt                                                                                                                                          >  1:1083607/1‑150 (MQ=255)
             cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                                                                          <  2:1353537/150‑1 (MQ=255)
             cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                                                                          <  1:2165306/150‑1 (MQ=255)
             cTGAAGTTGAGCTATCCCCAGAAGATTTAGAAATATTTGAAGGGCTTGTTGATGCCCTTGAAGATGAAGAAGATGTACAAAAAGTTTATCATAACTTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTATTGAAGGCCGATTTCttt                                                                                                                                          >  1:581969/1‑150 (MQ=255)
                 aGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAAATATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaaa                                                                                                                                      <  1:621239/150‑3 (MQ=39)
                  gTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaaag                                                                                                                                     <  2:2209389/150‑4 (MQ=39)
                    tGAGCTTTCCCCAGAAGTTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACTACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCa                                                                                                                                   <  2:228347/150‑1 (MQ=255)
                          ttCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACTATGTACAAAAACTTTATCATAACGTCGCAAATCTCTAATTATCATTAAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAAt                                                                                                                             <  1:356758/150‑1 (MQ=255)
                              ccAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCtt                                                                                                                         <  2:2027875/150‑1 (MQ=255)
                               cAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCttt                                                                                                                        >  1:895966/1‑149 (MQ=255)
                                agaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtat                                                                                                                       >  1:930791/1‑150 (MQ=255)
                                agaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCACTTGAAGATGACGACGATGTACAAAAAGTTTATATTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTATTTACGGCAGATTTATTTAATCTCATATAATtattat                                                                                                                       >  1:938848/1‑150 (MQ=255)
                                 gaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                      >  1:1809433/1‑150 (MQ=255)
                                 gaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                      <  2:1816802/150‑1 (MQ=255)
                                     aTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaaa                                                                                                                  <  2:1751053/150‑1 (MQ=255)
                                      tttAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaaaa                                                                                                                 <  2:758022/150‑1 (MQ=255)
                                       ttAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaat                                                                                                                <  2:1622010/150‑1 (MQ=255)
                                         aGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatat                                                                                                              <  2:510281/150‑1 (MQ=255)
                                            aaTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat                                                                                                           <  2:866479/150‑1 (MQ=255)
                                             aTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataata                                                                                                          <  1:547600/150‑1 (MQ=255)
                                                  tGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGAAGTCCAAAAAGTATATCATCACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTAAGCCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatatactatagac                                                                                                     >  1:92796/1‑146 (MQ=255)
                                                   gAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCaaa                                                                                                    >  1:1787123/1‑149 (MQ=255)
                                                   gAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAc                                                                                                    >  2:1493626/1‑150 (MQ=255)
                                                    aaGTGCTTGTTGATGCCCTTGAAGATGAAGACGATTTACAAAAAGTTTATCATAACGTCTCAAATCTCTAATTATATTTTAAAGAAATCTTTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACt                                                                                                   <  1:15825/150‑1 (MQ=255)
                                                     aGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTc                                                                                                  >  2:2129662/1‑150 (MQ=255)
                                                      gggCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCg                                                                                                 <  1:1844665/150‑1 (MQ=255)
                                                      gggCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCg                                                                                                 <  2:232707/150‑1 (MQ=255)
                                                        gCTTGTTGATGCCCTTGAAGTTGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGAACATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCTTc                                                                                               >  1:1031870/1‑150 (MQ=255)
                                                        gCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGta                                                                                               >  2:804951/1‑149 (MQ=255)
                                                             ttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATATCGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATAtt                                                                                          <  2:890211/150‑1 (MQ=255)
                                                             ttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATAtt                                                                                          >  1:1256844/1‑150 (MQ=255)
                                                              tgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTg                                                                                         >  1:434446/1‑150 (MQ=255)
                                                               gATGCCCTTGAAGATGACGACCATGTACAAAAAGTTAATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTAATATAATTCTTATAAAAAATATAATAATCAACTCGTCATATTGa                                                                                        >  2:27386/1‑150 (MQ=255)
                                                                 tGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCCCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGAtt                                                                                      >  2:945659/1‑150 (MQ=255)
                                                                   cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtat                                                                                    >  2:1328823/1‑150 (MQ=255)
                                                                    ccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtata                                                                                   >  2:1683100/1‑150 (MQ=255)
                                                                       tGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAccc                                                                                >  1:1145583/1‑150 (MQ=255)
                                                                              gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGtt                                                                         >  2:640955/1‑150 (MQ=255)
                                                                                        tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGaaa                                                               >  1:127629/1‑150 (MQ=255)
                                                                                         aCAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTAAACTCGTCATATTGATTATACCCCACCGTTCCCAGAGAaat                                                              >  2:763080/1‑150 (MQ=255)
                                                                                           aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataa                                                            >  1:1356679/1‑150 (MQ=255)
                                                                                           aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAATCGTCATATTGATTATACCCACCCGTTCCCAGAGAaatat                                                            >  2:896338/1‑149 (MQ=255)
                                                                                            aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataat                                                           >  1:1708469/1‑150 (MQ=255)
                                                                                               aGTTTATCATAACGTCGCAAATCTCTAATTATATTTTAAATAAATCTGTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatt                                                        <  1:1194339/150‑1 (MQ=255)
                                                                                                gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtattt                                                       <  1:2083040/150‑1 (MQ=255)
                                                                                                gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtattt                                                       >  1:1564066/1‑150 (MQ=255)
                                                                                                   tATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttatt                                                    <  1:774431/150‑1 (MQ=255)
                                                                                                   tATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAAAAAtatttatt                                                    >  2:594115/1‑150 (MQ=255)
                                                                                                    aTCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTACTTTAATATCATATAATTCTTATAAAAAATAAAATATTCAACTCCTCATATTGATTATACCCCCACGTTCCAACAGAAATAATATttaata                                                   >  2:1233890/1‑150 (MQ=255)
                                                                                                      cATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATTTTTATTaaa                                                 >  2:2146882/1‑150 (MQ=255)
                                                                                                      cATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTaaa                                                 >  1:1183232/1‑150 (MQ=255)
                                                                                                         aaCGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAAtt                                              <  1:1619164/150‑1 (MQ=255)
                                                                                                           cGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTcc                                            >  1:655878/1‑150 (MQ=255)
                                                                                                             tCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAg                                          <  1:220542/150‑1 (MQ=255)
                                                                                                              cGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCACAGAGAAATAATATTTATTAAAAtacaatt                                         >  1:2018272/1‑144 (MQ=255)
                                                                                                              agCAAATATATAATTATCTTTTAAAGAAATCTGTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGt                                         <  1:1552700/149‑1 (MQ=255)
                                                                                                                cAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttc                                       <  2:2209560/150‑1 (MQ=255)
                                                                                                                  aataaaaaataatATTTAAAATAAAACTGTATTACCGGCTTATTAATTAAATCTCAAAAAATCCTAAAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                    <  1:861900/143‑1 (MQ=255)
                                                                                                                   aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTTTAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                    >  2:934210/1‑150 (MQ=255)
                                                                                                                   aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                    <  1:1130287/150‑1 (MQ=255)
                                                                                                                    tctcTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTtcttct                                   <  2:1145583/150‑1 (MQ=255)
                                                                                                                       ctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                                >  2:30579/1‑150 (MQ=255)
                                                                                                                       ctAATTATCTTTTAAAGAAATCTGTCTTTAAGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                                <  1:1855753/150‑1 (MQ=255)
                                                                                                                       ctAATTATCTTTTAAAGAAATCTGTCTTAAAGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                                <  1:1094886/150‑1 (MQ=255)
                                                                                                                       atAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                                <  1:660866/149‑1 (MQ=255)
                                                                                                                        tAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttttt                               <  1:1573164/150‑1 (MQ=255)
                                                                                                                         aaTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc                              <  1:1482018/150‑1 (MQ=255)
                                                                                                                         aaTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCAAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttttg                              >  2:947789/1‑149 (MQ=255)
                                                                                                                          aTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCt                             <  1:1328823/150‑1 (MQ=255)
                                                                                                                           ttATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTg                            <  2:1673333/150‑1 (MQ=255)
                                                                                                                             aTCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATATCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAt                          <  2:118582/150‑1 (MQ=255)
                                                                                                                               cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa                        >  2:1525781/1‑150 (MQ=255)
                                                                                                                                ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATTTTTATTAAAATTCCAGTTCTTCTTTTTCTGATTaa                       >  1:558958/1‑149 (MQ=255)
                                                                                                                                ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc                       >  1:528147/1‑150 (MQ=255)
                                                                                                                                gattAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCACACGTTCCCAGAGAAATAATATTTATTAAAATTCAAGTTCTTATTTTTCTGATTaa                       >  2:655301/3‑149 (MQ=38)
                                                                                                                                 tttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAGTACa                      >  2:1744416/1‑150 (MQ=255)
                                                                                                                                  ttAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCACCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTAATTACAg                     >  2:1905515/1‑150 (MQ=255)
                                                                                                                                    aaaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa                   >  2:478470/1‑150 (MQ=255)
                                                                                                                                     aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGATATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                  >  2:616871/1‑150 (MQ=255)
                                                                                                                                     aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAATTTCCAGTTCTTCTTTTTCTGATTACAGAAg                  >  2:1258601/1‑150 (MQ=255)
                                                                                                                                     aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAAAAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                  <  1:1462994/150‑1 (MQ=255)
                                                                                                                                     aaGAAATCTGCCGTAAAGACAGATTTCTGTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATTTTGATTATACCCCCCCGTGCCCAGAGAAATAATATATATTTAAATTCAAGTTGTGCTTTTTCTGATTACAGAAg                  >  1:758297/1‑150 (MQ=255)
                                                                                                                                      aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAgg                 <  1:1722274/150‑1 (MQ=255)
                                                                                                                                      aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAgg                 <  2:2104455/150‑1 (MQ=255)
                                                                                                                                        aaaTCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCa               <  2:670458/150‑1 (MQ=255)
                                                                                                                                                cTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCa       >  2:1985448/1‑150 (MQ=255)
                                                                                                                                                cTTTACGGCAGATTTCTTTAATCTCATATAATTATTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTATTCTTTTACTGATTACATAAGGCAAAGTggaa       >  2:1473761/1‑148 (MQ=255)
                                                                                                                                                   tACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAAtt    >  1:474318/1‑150 (MQ=255)
                                                                                                                                                    aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCACACCGTTCCCAGAGAAATAATATTTATTAAAATTCAAGTTATTCTTTTTCTGATTACATAAGGCAAAGTGTCAATTa   >  1:337060/1‑150 (MQ=255)
                                                                                                                                                     cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCAAGTTCTTATTTTTCTGATTACAGAAGTCAAATTTGCAATTaa  >  1:2156812/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTAC  >  NC_000913/2057452‑2057750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: