Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,057,603 G→A 6.7% intergenic (+29/+424) yeeN → / ← asnW UPF0082 family protein/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,057,6030GA 6.7% 355.4 / 10.5 123intergenic (+29/+424)yeeN/asnWUPF0082 family protein/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base G (62/50);  new base A (4/4);  total (69/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.96e-01

Only 100 of 128 total aligned reads displayed.

GATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGC  >  NC_000913/2057454‑2057752
                                                                                                                                                     |                                                                                                                                                     
taTTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACgg                                                                                                                                                       <  1:608112/149‑1 (MQ=255)
gATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACgg                                                                                                                                                       <  2:523472/150‑1 (MQ=255)
gATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACgg                                                                                                                                                       <  2:1569448/150‑1 (MQ=255)
    gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTTTCTTTACGCCAGa                                                                                                                                                   >  2:379639/1‑150 (MQ=255)
    gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGa                                                                                                                                                   >  2:1573164/1‑150 (MQ=255)
       cAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAATGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAttt                                                                                                                                                >  1:48777/1‑150 (MQ=255)
         aTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCt                                                                                                                                              <  1:512101/150‑1 (MQ=255)
          tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCtt                                                                                                                                             <  1:2129669/150‑1 (MQ=255)
          tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCtt                                                                                                                                             <  2:1957026/150‑1 (MQ=255)
           cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTTTCTTTACGGAAGATTTCttt                                                                                                                                            >  1:1083607/1‑150 (MQ=255)
           cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                                                                            <  1:2165306/150‑1 (MQ=255)
           cTGAAGTTGAGCTATCCCCAGAAGATTTAGAAATATTTGAAGGGCTTGTTGATGCCCTTGAAGATGAAGAAGATGTACAAAAAGTTTATCATAACTTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTATTGAAGGCCGATTTCttt                                                                                                                                            >  1:581969/1‑150 (MQ=255)
               aGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAAATATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaaa                                                                                                                                        <  1:621239/150‑3 (MQ=39)
                gTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaaag                                                                                                                                       <  2:2209389/150‑4 (MQ=39)
                  tGAGCTTTCCCCAGAAGTTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACTACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCa                                                                                                                                     <  2:228347/150‑1 (MQ=255)
                      cTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAATGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTTCGGCAGATTTCTTTAATATCAtata                                                                                                                                 >  2:131713/1‑150 (MQ=255)
                        ttCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACTATGTACAAAAACTTTATCATAACGTCGCAAATCTCTAATTATCATTAAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAAt                                                                                                                               <  1:356758/150‑1 (MQ=255)
                             cAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCttt                                                                                                                          >  1:895966/1‑149 (MQ=255)
                              agaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtat                                                                                                                         >  1:930791/1‑150 (MQ=255)
                              agaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCACTTGAAGATGACGACGATGTACAAAAAGTTTATATTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTATTTACGGCAGATTTATTTAATCTCATATAATtattat                                                                                                                         >  1:938848/1‑150 (MQ=255)
                               gaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                        >  1:1748630/1‑150 (MQ=255)
                               gaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                        <  2:1816802/150‑1 (MQ=255)
                               gaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                        <  2:1733417/150‑1 (MQ=255)
                                    tttAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaaaa                                                                                                                   <  2:758022/150‑1 (MQ=255)
                                     ttAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaat                                                                                                                  <  2:1622010/150‑1 (MQ=255)
                                      tAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGAAGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAAGCTGTCGTTACGGCACATTTCTTTAATCTCATATAATTCTTATaaaaaagt                                                                                                                 >  2:2208705/1‑148 (MQ=255)
                                       aGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatat                                                                                                                <  2:2079101/150‑1 (MQ=255)
                                       aGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatat                                                                                                                <  2:510281/150‑1 (MQ=255)
                                          aaTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat                                                                                                             >  1:1247207/1‑150 (MQ=255)
                                          aaTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat                                                                                                             <  2:866479/150‑1 (MQ=255)
                                           aTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataata                                                                                                            <  1:547600/150‑1 (MQ=255)
                                            tCTTTGAAGGTCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAACACTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataatat                                                                                                           <  2:264031/150‑1 (MQ=255)
                                              tttGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTc                                                                                                         <  1:704742/150‑1 (MQ=255)
                                                 gAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCaaa                                                                                                      >  1:1787123/1‑149 (MQ=255)
                                                 gAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAc                                                                                                      >  2:1493626/1‑150 (MQ=255)
                                                  aaGTGCTTGTTGATGCCCTTGAAGATGAAGACGATTTACAAAAAGTTTATCATAACGTCTCAAATCTCTAATTATATTTTAAAGAAATCTTTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACt                                                                                                     <  1:15825/150‑1 (MQ=255)
                                                   aGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTc                                                                                                    >  2:2129662/1‑150 (MQ=255)
                                                    gggCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCg                                                                                                   <  1:1844665/150‑1 (MQ=255)
                                                      gCTTGTTGATGCCCTTGAAGTTGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGAACATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCTTc                                                                                                 >  1:1031870/1‑150 (MQ=255)
                                                           ttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATATCGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATAtt                                                                                            <  2:890211/150‑1 (MQ=255)
                                                            tgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTg                                                                                           >  1:434446/1‑150 (MQ=255)
                                                             gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTATAAAAAATATAATATTCAACTCGTCATATTGAt                                                                                         >  1:910175/1‑150 (MQ=255)
                                                             gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTAGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGa                                                                                          <  2:828524/150‑1 (MQ=255)
                                                             gATGCCCTTGAAGATGACGACCATGTACAAAAAGTTAATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTAATATAATTCTTATAAAAAATATAATAATCAACTCGTCATATTGa                                                                                          >  2:27386/1‑150 (MQ=255)
                                                               tGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCCCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGAtt                                                                                        >  2:945659/1‑150 (MQ=255)
                                                                 cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtat                                                                                      >  2:1328823/1‑150 (MQ=255)
                                                                  ccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtata                                                                                     >  2:1683100/1‑150 (MQ=255)
                                                                     tGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAccc                                                                                  >  1:1145583/1‑150 (MQ=255)
                                                                      gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccc                                                                                 >  1:1613424/1‑150 (MQ=255)
                                                                            gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGtt                                                                           >  2:640955/1‑150 (MQ=255)
                                                                                  gaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCaga                                                                     <  1:1496952/150‑1 (MQ=255)
                                                                                    tGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAgaga                                                                   <  1:1920883/150‑1 (MQ=255)
                                                                                      tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGaaa                                                                 >  1:127629/1‑150 (MQ=255)
                                                                                       aCAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTAAACTCGTCATATTGATTATACCCCACCGTTCCCAGAGAaat                                                                >  2:763080/1‑150 (MQ=255)
                                                                                        cAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAaata                                                               >  2:1608624/1‑150 (MQ=255)
                                                                                         aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataa                                                              >  1:1356679/1‑150 (MQ=255)
                                                                                         aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAATCGTCATATTGATTATACCCACCCGTTCCCAGAGAaatat                                                              >  2:896338/1‑149 (MQ=255)
                                                                                          aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataat                                                             >  1:1708469/1‑150 (MQ=255)
                                                                                             aGTTTATCATAACGTCGCAAATCTCTAATTATATTTTAAATAAATCTGTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatt                                                          <  1:1194339/150‑1 (MQ=255)
                                                                                              gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtattt                                                         <  1:2083040/150‑1 (MQ=255)
                                                                                                 tATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttatt                                                      <  1:774431/150‑1 (MQ=255)
                                                                                                  aTCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTACTTTAATATCATATAATTCTTATAAAAAATAAAATATTCAACTCCTCATATTGATTATACCCCCACGTTCCAACAGAAATAATATttaata                                                     >  2:1233890/1‑150 (MQ=255)
                                                                                                    cATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTaaa                                                   >  1:1183232/1‑150 (MQ=255)
                                                                                                           tCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAg                                            <  1:220542/150‑1 (MQ=255)
                                                                                                            cGCAAATCTCTAATTATCTTTTAAAGAAATCTTTCTTTACGGCAGATTTCTTTAATCTCATATAATTCGTATAAAAAATATAATATTCAACTCGTAATATTGATTATACCACCGCTTTCCCATAGATATAATATTTATTAAAATTCCAGt                                           >  2:1045830/1‑150 (MQ=255)
                                                                                                            agCAAATATATAATTATCTTTTAAAGAAATCTGTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGt                                           <  1:1552700/149‑1 (MQ=255)
                                                                                                             gCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCTTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGtt                                          >  2:611431/1‑150 (MQ=255)
                                                                                                              cAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttc                                         <  2:2209560/150‑1 (MQ=255)
                                                                                                                aataaaaaataatATTTAAAATAAAACTGTATTACCGGCTTATTAATTAAATCTCAAAAAATCCTAAAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                      <  1:861900/143‑1 (MQ=255)
                                                                                                                 aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTTTAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                      >  2:934210/1‑150 (MQ=255)
                                                                                                                 aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                      <  1:1130287/150‑1 (MQ=255)
                                                                                                                  tctcTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAATATATAATATTCAACTCGTCATATTGATTATACCCCCACGTTCCCAGAGTAATAATATTTATTAAAATTCAAGTtgttct                                     >  1:386890/1‑150 (MQ=255)
                                                                                                                  tctcTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTtcttct                                     <  2:1145583/150‑1 (MQ=255)
                                                                                                                     ctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                                  >  2:30579/1‑150 (MQ=255)
                                                                                                                     ctAATTATCTTTTAAAGAAATCTGTCTTTAAGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                                  <  1:1855753/150‑1 (MQ=255)
                                                                                                                     ctAATTATCTTTTAAAGAAATCTGTCTTAAAGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                                  <  1:1094886/150‑1 (MQ=255)
                                                                                                                     atAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                                  <  1:660866/149‑1 (MQ=255)
                                                                                                                      tAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttttt                                 <  1:1573164/150‑1 (MQ=255)
                                                                                                                       aaTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc                                <  1:1482018/150‑1 (MQ=255)
                                                                                                                       aaTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCAAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttttg                                >  2:947789/1‑149 (MQ=255)
                                                                                                                       aaTTATCTTTTAAAGAAATATGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc                                <  1:309384/150‑1 (MQ=255)
                                                                                                                        aTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCt                               <  1:1328823/150‑1 (MQ=255)
                                                                                                                              ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc                         >  1:528147/1‑150 (MQ=255)
                                                                                                                              gattAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCACACGTTCCCAGAGAAATAATATTTATTAAAATTCAAGTTCTTATTTTTCTGATTaa                         >  2:655301/3‑149 (MQ=38)
                                                                                                                               tttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAGTACa                        >  2:1744416/1‑150 (MQ=255)
                                                                                                                                ttAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCACCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTAATTACAg                       >  2:1905515/1‑150 (MQ=255)
                                                                                                                                  taattaaTCTTTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                    <  1:2119752/145‑1 (MQ=255)
                                                                                                                                  aaaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa                     >  2:478470/1‑150 (MQ=255)
                                                                                                                                   aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGATATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                    >  2:616871/1‑150 (MQ=255)
                                                                                                                                   aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAATTTCCAGTTCTTCTTTTTCTGATTACAGAAg                    >  2:1258601/1‑150 (MQ=255)
                                                                                                                                   aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAAAAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                    <  1:1462994/150‑1 (MQ=255)
                                                                                                                                   aaGAAATCTGCCGTAAAGACAGATTTCTGTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATTTTGATTATACCCCCCCGTGCCCAGAGAAATAATATATATTTAAATTCAAGTTGTGCTTTTTCTGATTACAGAAg                    >  1:758297/1‑150 (MQ=255)
                                                                                                                                    aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAgg                   <  1:1722274/150‑1 (MQ=255)
                                                                                                                                    aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAgg                   <  2:1492137/150‑1 (MQ=255)
                                                                                                                                    aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAgg                   <  2:2104455/150‑1 (MQ=255)
                                                                                                                                      aaaTCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCa                 <  2:670458/150‑1 (MQ=255)
                                                                                                                                              cTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCa         >  2:1985448/1‑150 (MQ=255)
                                                                                                                                              cTTTACGGCAGATTTCTTTAATCTCATATAATTATTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTATTCTTTTACTGATTACATAAGGCAAAGTggaa         >  2:1473761/1‑148 (MQ=255)
                                                                                                                                                  aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCACACCGTTCCCAGAGAAATAATATTTATTAAAATTCAAGTTATTCTTTTTCTGATTACATAAGGCAAAGTGTCAATTa     >  1:337060/1‑150 (MQ=255)
                                                                                                                                                     gCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTAAACTCGTCATATTGATTATACACCCCAGTTCCCAGAGAAATATTATTTATTAAAATTCCAGTTCTTCTTTTTATGATTACAGAATGCAAAGTGGCAAGTActa  >  1:2129189/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGC  >  NC_000913/2057454‑2057752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: