Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,113,368 T→A 5.1% intergenic (‑307/+66) rfbB ← / ← wcaN dTDP‑glucose 4,6 dehydratase, NAD(P)‑binding/putative regulatory subunit for GalU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,113,3680TA 5.1% 425.0 / 7.2 135intergenic (‑307/+66)rfbB/wcaNdTDP‑glucose 4,6 dehydratase, NAD(P)‑binding/putative regulatory subunit for GalU
Reads supporting (aligned to +/- strand):  ref base T (60/68);  new base A (2/5);  total (62/73)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 141 total aligned reads displayed.

CCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTG  >  NC_000913/2113220‑2113517
                                                                                                                                                     |                                                                                                                                                     
cccGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACtt                                                                                                                                                      >  2:1652316/1‑150 (MQ=255)
 ccGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACttt                                                                                                                                                     >  1:1532204/1‑150 (MQ=255)
  cGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACtttt                                                                                                                                                    >  1:764794/1‑150 (MQ=255)
  cGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACtttt                                                                                                                                                    <  1:2052353/150‑1 (MQ=255)
    aaaaaaTCAAACGCTAATTGTCTTAACAATCCGCGCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAATACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTtgc                                                                                                                                                  <  2:339584/150‑1 (MQ=255)
    aaaaaTTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTtgc                                                                                                                                                  <  2:2097047/150‑1 (MQ=255)
     aaaaTTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTtgct                                                                                                                                                 >  2:1826277/1‑150 (MQ=255)
        aTTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCg                                                                                                                                              >  2:1790502/1‑150 (MQ=255)
            aaaCGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGTAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAAtt                                                                                                                                          <  1:1412500/150‑1 (MQ=255)
               cGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTc                                                                                                                                       <  1:1532156/150‑1 (MQ=255)
                gCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAGCAAAAGTAATTTGTTGCGAATCTTcc                                                                                                                                      <  2:1725055/150‑2 (MQ=38)
                   aaTTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTCATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTAGCTGCGAATTTTCACTg                                                                                                                                   >  2:299270/1‑150 (MQ=255)
                    aTTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGTGAAAATTCGCAGCAAAAGTAATTTGTTGCGAATCTTcctgc                                                                                                                                   <  1:2025949/150‑5 (MQ=25)
                    aTTGTCTTAACAATCCGCTCTGGAAACAAGGATAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGTGAAACTTCGCAGCAAAAGTAATTTGTTGCGAATCTTcctgc                                                                                                                                   <  1:317746/150‑5 (MQ=25)
                     ttGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGTGAAAATTCGCAGCAAAAGTAATTTGTTGCGAATCTTCCTGcc                                                                                                                                 <  2:2208256/150‑1 (MQ=25)
                     ttGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGcc                                                                                                                                 <  1:1913735/150‑1 (MQ=255)
                      tGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGTGAAAATTCGCAGCAAAAGTAATTTGTTGCGAATCTTCCTGCCg                                                                                                                                <  2:878492/150‑1 (MQ=25)
                      tGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCg                                                                                                                                >  2:480438/1‑150 (MQ=255)
                      tGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCg                                                                                                                                >  2:1033497/1‑150 (MQ=255)
                      tGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCg                                                                                                                                >  1:69719/1‑150 (MQ=255)
                             ccAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAAt                                                                                                                         >  1:1670446/1‑150 (MQ=255)
                             ccAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAAt                                                                                                                         >  1:456957/1‑150 (MQ=255)
                               aaTCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAAttt                                                                                                                       >  1:2113166/1‑150 (MQ=255)
                                   cGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCtt                                                                                                                   <  2:719972/150‑1 (MQ=255)
                                    gCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTa                                                                                                                  <  2:1003629/150‑1 (MQ=255)
                                    gCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTa                                                                                                                  <  1:805525/150‑1 (MQ=255)
                                           aagcAAGGAAAATCCTGGAAAACATTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACTGCAGGAAGATTCTCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCg                                                                                                           <  1:109450/147‑1 (MQ=255)
                                           aaaCAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCg                                                                                                           <  1:1857242/150‑1 (MQ=255)
                                           aaaCAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCg                                                                                                           <  1:488670/150‑1 (MQ=255)
                                             aCAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGtt                                                                                                         <  2:497386/150‑1 (MQ=255)
                                              cAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTa                                                                                                        <  1:906498/150‑1 (MQ=255)
                                              cAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTa                                                                                                        >  1:1056720/1‑150 (MQ=255)
                                              aaaGGAAAATCCTTGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTa                                                                                                        <  2:491441/149‑1 (MQ=255)
                                                 ggAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACAt                                                                                                     >  1:1719949/1‑150 (MQ=255)
                                                 ggAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACAt                                                                                                     >  1:447781/1‑150 (MQ=255)
                                                     aaTCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCgg                                                                                                 <  1:517368/150‑1 (MQ=255)
                                                      aTCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGt                                                                                                <  2:878206/150‑1 (MQ=255)
                                                       tCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTc                                                                                               >  2:437898/1‑150 (MQ=255)
                                                         cTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTctg                                                                                             >  2:2021710/1‑148 (MQ=255)
                                                           ggAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCTGAt                                                                                           >  2:532289/1‑150 (MQ=255)
                                                           ggAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGAt                                                                                           >  1:2063449/1‑150 (MQ=255)
                                                             aaaaCTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGAttt                                                                                         >  1:1779475/1‑150 (MQ=255)
                                                             aaaaCTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATAAACCGTTACATCCGGTCAGAttt                                                                                         >  2:204083/1‑150 (MQ=255)
                                                               aaCTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTc                                                                                       <  2:764794/150‑1 (MQ=255)
                                                                aCTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCa                                                                                      <  2:1803621/150‑1 (MQ=255)
                                                                aCTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCa                                                                                      >  1:1466060/1‑150 (MQ=255)
                                                                 cTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCat                                                                                     >  2:955164/1‑150 (MQ=255)
                                                                 atttGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCat                                                                                     <  1:2028069/149‑1 (MQ=255)
                                                                  tttGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCatt                                                                                    >  1:535069/1‑150 (MQ=255)
                                                                   ttGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCatta                                                                                   >  2:1082056/1‑150 (MQ=255)
                                                                        tAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCAGGTCAGATTTTCATTATTCGc                                                                              >  2:253565/1‑150 (MQ=255)
                                                                         aaacCCCCACTCCTAACTCCTTGTTATTCTGATGGTCTATATAAAACAACGGCAGGAAGAATCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCt                                                                             <  2:1466060/150‑1 (MQ=255)
                                                                         aaaaCCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCt                                                                             <  2:398907/150‑1 (MQ=255)
                                                                         aaaaCCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCt                                                                             <  2:1425017/150‑1 (MQ=255)
                                                                         aaaaCCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGGTACATCCGGTCAGATTTTCATTATTCGCt                                                                             <  2:15951/150‑1 (MQ=255)
                                                                           aaCCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTa                                                                           <  1:1826277/150‑1 (MQ=255)
                                                                           aaCCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTa                                                                           <  2:1189641/150‑1 (MQ=255)
                                                                              ccTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACa                                                                        <  2:1577351/150‑1 (MQ=255)
                                                                                  cTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCTGTCAGATTTTCATTATTCGCTTAACAGCtt                                                                    >  1:100810/1‑150 (MQ=255)
                                                                                    gCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTtct                                                                  >  1:848167/1‑150 (MQ=255)
                                                                                    gCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTtct                                                                  >  1:1465092/1‑150 (MQ=255)
                                                                                    gCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATAAACCGTTACATACGGTCAGATTTTCATTATTCGCTTAACAGCTtct                                                                  >  1:1988222/1‑150 (MQ=255)
                                                                                      tAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCa                                                                <  1:1533472/150‑1 (MQ=255)
                                                                                      tAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCa                                                                <  1:1239167/150‑1 (MQ=255)
                                                                                      tAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCa                                                                <  1:1033497/150‑1 (MQ=255)
                                                                                      tAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCa                                                                <  1:382572/150‑1 (MQ=255)
                                                                                        aCTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAAt                                                              >  2:616994/1‑150 (MQ=255)
                                                                                         cTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATa                                                             >  1:652357/1‑150 (MQ=255)
                                                                                          tCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATAc                                                            <  2:2121996/150‑1 (MQ=255)
                                                                                          tCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATAc                                                            <  1:1678719/150‑1 (MQ=255)
                                                                                           cGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATAcc                                                           <  1:621692/150‑1 (MQ=255)
                                                                                               gttATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTa                                                       >  1:1012077/1‑150 (MQ=255)
                                                                                                  aTTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACgg                                                    <  1:1719576/150‑1 (MQ=255)
                                                                                                   ttCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAAATACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGAGTTTCAGTATTCGCTTAACAGCTTCTCATTACCTTTACGGa                                                   >  2:620497/1‑150 (MQ=255)
                                                                                                     cTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAAc                                                 >  2:1862002/1‑150 (MQ=255)
                                                                                                      tGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTAc                                                      >  2:654481/1‑144 (MQ=255)
                                                                                                      tGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTAc                                                      <  1:654481/144‑1 (MQ=255)
                                                                                                        aTGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTc                                              <  1:1249617/150‑1 (MQ=255)
                                                                                                        aTGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTc                                              >  2:2171468/1‑150 (MQ=255)
                                                                                                         tGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCg                                             >  2:352719/1‑150 (MQ=255)
                                                                                                           gTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGcc                                           <  2:1454662/150‑1 (MQ=255)
                                                                                                            ttttTATAAAACAACGGAAGGAAGATTCGAAAGATTTTACTTTTGCTGCGAATTTTAACTGCAGTTATAATTTTCTTATCAACCGTTACATCCGTTCAGATTTTCATTATTCGCTTAACAGCTTGTCAATACCTTTAGGGATCttatccc                                          <  2:1424474/150‑6 (MQ=255)
                                                                                                                tataAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCcttg                                      >  1:1707418/1‑149 (MQ=255)
                                                                                                                tataAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCcttc                                      >  1:695871/1‑150 (MQ=255)
                                                                                                                 ataAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCcttct                                     <  1:1790502/150‑1 (MQ=255)
                                                                                                                 ataAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCcttct                                     >  2:1967223/1‑150 (MQ=255)
                                                                                                                  taAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGATTTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGACCcttctt                                    >  1:211660/1‑150 (MQ=255)
                                                                                                                  taAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAAGAGCTTCTCAATACCTTTACGGAACTTCGCCCcttctt                                    >  2:624426/1‑150 (MQ=255)
                                                                                                                  taAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCcttctt                                    >  2:680259/1‑150 (MQ=255)
                                                                                                                   aaaaCAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCttt                                   <  1:2071060/150‑1 (MQ=255)
                                                                                                                    aaaCAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTc                                  >  1:629909/1‑150 (MQ=255)
                                                                                                                    aaaCAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTc                                  >  2:1756516/1‑150 (MQ=255)
                                                                                                                     tataacGGCAGTGAAAATTCGCAGCAAAAGTAATTTGTTGCGAATCTTCCTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCa                                 >  2:1230330/4‑150 (MQ=21)
                                                                                                                             ctggAAGATTCTCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGt                         <  1:299270/148‑1 (MQ=255)
                                                                                                                             cAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCTTTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGt                         <  2:1599319/150‑1 (MQ=255)
                                                                                                                                  aaGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGc                     >  2:972621/1‑150 (MQ=255)
                                                                                                                                   aGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGcc                    <  2:1206678/150‑1 (MQ=255)
                                                                                                                                        cGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACt               >  1:1900151/1‑150 (MQ=255)
                                                                                                                                                 ttACTTTTGCTGCGAATTTTCAATGCAGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACg      <  2:2073597/150‑1 (MQ=255)
                                                                                                                                                  tACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGc     <  2:1056720/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTG  >  NC_000913/2113220‑2113517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: