Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,139,727 T→C 5.5% intergenic (+242/+32) yegH → / ← asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,139,7270TC 5.5% 451.7 / 11.1 146intergenic (+242/+32)yegH/asmAinner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (70/68);  new base C (4/4);  total (74/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 152 total aligned reads displayed.

TGGTGGAGATTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGA  >  NC_000913/2139578‑2139876
                                                                                                                                                     |                                                                                                                                                     
  gtggAGATTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTAt                                                                                                                                                     >  1:1314902/1‑150 (MQ=255)
   tggAGATTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATc                                                                                                                                                    >  1:862441/1‑150 (MQ=255)
    ggAGATTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTTGCGAGGAACATCAAGTGATCGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCa                                                                                                                                                   <  2:1214641/150‑1 (MQ=255)
    ggAGATTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTCCGCGTCTTATCa                                                                                                                                                   >  2:382880/1‑150 (MQ=255)
      agaTTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGGTGCCGCCACGAACGCCTGATGCGACCCTTTACGCGTCTTATCAgg                                                                                                                                                 >  1:1195189/1‑150 (MQ=255)
       gaTTGAGCCACCCGGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGc                                                                                                                                                <  1:451684/150‑1 (MQ=255)
        aTTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGcc                                                                                                                                               <  2:1581247/150‑1 (MQ=255)
        aTTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGcc                                                                                                                                               <  2:729047/150‑1 (MQ=255)
        aTTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGcc                                                                                                                                               <  2:588724/150‑1 (MQ=255)
         gggcgCCCCCCGGGCACGTTCACCGGCTGGAGCGTTCCATCGTTGCGAGGAACATCAAGGGAACGGAACAGCCTTCGATCTTTCATCTGCCGAATCTAATGATGCCGCCACGAACGCCTGATAACACGCGTAAAGCGTCGCATCAGGcgt                                                                                                                                              <  1:1110284/146‑3 (MQ=255)
            aGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACa                                                                                                                                           <  1:1069426/150‑1 (MQ=255)
            aGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACa                                                                                                                                           <  1:812733/150‑1 (MQ=255)
            aGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATAAGACGCGTAAAGCGTCGCATCAGGcgttcg                                                                                                                                           <  2:1640242/150‑6 (MQ=38)
             gCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCATTACGCGTCTTATCAGGCCTACAg                                                                                                                                          >  1:1719076/1‑150 (MQ=255)
                aCCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGAc                                                                                                                                       >  1:231857/1‑150 (MQ=255)
                 cccTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACt                                                                                                                                      >  2:2156949/1‑150 (MQ=255)
                 cccTGGCACGTTAACCGGCTTGAGCGTTCCATAGTAGCGAGTAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATAAAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTAATATCAGACCAACAGGACt                                                                                                                                      >  1:75032/1‑150 (MQ=255)
                  ccTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTg                                                                                                                                     <  2:524557/150‑1 (MQ=255)
                     ggCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTc                                                                                                                                  >  1:828020/1‑150 (MQ=255)
                     ggCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTc                                                                                                                                  <  1:1657014/150‑1 (MQ=255)
                          gTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTg                                                                                                                             <  1:283912/150‑1 (MQ=255)
                           ttCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTgg                                                                                                                            <  2:2063537/150‑1 (MQ=255)
                           ttCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTgg                                                                                                                            <  1:1022364/150‑1 (MQ=255)
                             cACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGACCGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTa                                                                                                                          >  1:213539/1‑150 (MQ=255)
                              aCCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCATGATGCGACGCTTTACGCGTCTTATCAGTCCTACAGGACTGGTCACCTGGTaa                                                                                                                         >  1:157811/1‑150 (MQ=255)
                                cGGCTTGAGCGTTCCTTAGTAGCGAGGATCTTCAAGTGCACGGAACTGCCTTCGTTCTTACATATTCAGATTTTAATGATGCCGCGACGAACGCCTGATGCGACGCTTTACGCGTCTTATCTGGCCTACAGGACTGGTCACCTGGTAAcc                                                                                                                       <  1:182785/150‑1 (MQ=255)
                                   cTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCCCGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCtcc                                                                                                                    >  2:193739/1‑148 (MQ=255)
                                   cTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTAc                                                                                                                    >  1:2096439/1‑150 (MQ=255)
                                    ttGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACa                                                                                                                   <  1:1171011/150‑1 (MQ=255)
                                     tGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGCACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTACCCTACAt                                                                                                                  >  2:345858/1‑150 (MQ=255)
                                     tGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAt                                                                                                                  >  1:1743172/1‑150 (MQ=255)
                                        gCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtctt                                                                                                               >  2:177646/1‑150 (MQ=255)
                                         cGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtcttc                                                                                                              <  1:321412/150‑1 (MQ=255)
                                          gTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtcttct                                                                                                             >  2:1829175/1‑150 (MQ=255)
                                             ccATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCa                                                                                                          <  2:1074305/150‑1 (MQ=255)
                                              cATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAGCTTCTCCAg                                                                                                         >  2:2124513/1‑150 (MQ=255)
                                               aTAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGc                                                                                                        <  1:1594618/150‑1 (MQ=255)
                                                tagtagCGCGGAACATCAAGTGTACGGAACAGCCTTCGTTCTTACTTATTCAGAATATAATGATGACGCCACGATCGACTTATGAGACGCTTTACTCGTCTTATCAGGCCTACAGGACTGGTCAGCTGGTAACCTACATCTTCTACAGCa                                                                                                       <  1:130935/150‑1 (MQ=255)
                                                  gtagCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAAc                                                                                                     <  2:34801/150‑1 (MQ=255)
                                                        aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACttcttc                                                                                               >  2:1446609/1‑150 (MQ=255)
                                                         ggAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTca                                                                                              <  1:441117/150‑1 (MQ=255)
                                                            aCATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACAt                                                                                           >  1:1976499/1‑150 (MQ=255)
                                                             cATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATc                                                                                          >  2:1144889/1‑150 (MQ=255)
                                                             cATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATc                                                                                          >  2:191408/1‑150 (MQ=255)
                                                                cAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTACCCTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                       >  2:1293241/1‑150 (MQ=255)
                                                                 aaGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTg                                                                                      <  2:732915/150‑1 (MQ=255)
                                                                  aGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGc                                                                                     >  2:1041208/1‑150 (MQ=255)
                                                                   gTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATATTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGGAACCTACATCTTCTCCAGCAACTTCTTCACATCTGTGcc                                                                                    >  2:1408349/1‑150 (MQ=255)
                                                                     gAACGGAACAGCCTTAGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGATTTACGCGTCTTATCAGGACTACAGGACTGGTCACCTGGTAACATACATATTCTCCAGCTACTTCTACACATCTTTGCCAt                                                                                  >  1:304450/1‑150 (MQ=255)
                                                                             cAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCTACTTCTTCACAGCTTTGCCATTACGGGat                                                                          >  2:2106813/1‑149 (MQ=255)
                                                                             cAGCCTTCGATCTTACATAATCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGaa                                                                          <  1:896610/150‑1 (MQ=255)
                                                                                 cTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTACCCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCtt                                                                      >  1:597189/1‑150 (MQ=255)
                                                                                 cTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCtt                                                                      >  2:611145/1‑150 (MQ=255)
                                                                                  ttCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCttt                                                                     <  1:685177/150‑1 (MQ=255)
                                                                                   tCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTa                                                                    >  1:2024910/1‑150 (MQ=255)
                                                                                    cGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTAt                                                                   <  2:2121380/150‑1 (MQ=255)
                                                                                     gATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTGTAtt                                                                  >  2:383848/1‑150 (MQ=255)
                                                                                       tCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTcc                                                                <  2:874612/150‑1 (MQ=255)
                                                                                         ttACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGc                                                              <  1:722022/150‑1 (MQ=255)
                                                                                         ttACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGc                                                              <  1:564080/150‑1 (MQ=255)
                                                                                               atTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAGTCTTTATTCCGCTCGGcc                                                        >  1:1474769/1‑150 (MQ=255)
                                                                                               atTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGcc                                                        >  1:870034/1‑150 (MQ=255)
                                                                                               atTCAGAATATAATAATGCCGCCACGAACGCCTGATGCGACGATTTACGCATCTTATCAGGCATACAGGACTAGTAACCTGGTAACCGACATCTTCTACAGCAACTTATTCACATCTTTGTCATTACGCGAATCTTTATTCCGCtagtca                                                        >  2:156949/1‑145 (MQ=255)
                                                                                                ttCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGccc                                                       >  1:1480668/1‑150 (MQ=255)
                                                                                                ttCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGccc                                                       >  1:1913118/1‑150 (MQ=255)
                                                                                                  cAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAg                                                     >  1:1773469/1‑150 (MQ=255)
                                                                                                       tataATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAtt                                                >  1:1843332/1‑150 (MQ=255)
                                                                                                         taATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCCCCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCa                                              >  2:1918754/1‑150 (MQ=255)
                                                                                                         taATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCa                                              >  2:525924/1‑150 (MQ=255)
                                                                                                         taATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCACGGCCCAGTCATTCa                                              >  1:791681/1‑150 (MQ=255)
                                                                                                           atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAgg                                            <  2:2165365/150‑1 (MQ=255)
                                                                                                             gatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCg                                          <  2:684062/150‑1 (MQ=255)
                                                                                                             gatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCg                                          <  1:782712/150‑1 (MQ=255)
                                                                                                                  cgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGtt                                     <  1:1102861/150‑1 (MQ=255)
                                                                                                                  cgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGtt                                     >  2:298426/1‑150 (MQ=255)
                                                                                                                    ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTc                                   <  1:683209/150‑1 (MQ=255)
                                                                                                                     cACGAACGCCTGATGCGCCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCATCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCGGTCCGGCGACGTTTCg                                  >  2:2128869/1‑150 (MQ=255)
                                                                                                                          aggCCTGATAAGACGCGTAAAGCGTCGCATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                             >  1:1966687/3‑150 (MQ=255)
                                                                                                                          aCGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                             <  2:1200491/150‑1 (MQ=255)
                                                                                                                          aCGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAAGCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                             <  1:193739/150‑1 (MQ=255)
                                                                                                                           ggCCTGATAAGACGCGTAAAGCGTCGCATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCCGCCCAGTCTTTCAGGCGACGTTTCGCTTCGt                            >  1:407838/2‑150 (MQ=255)
                                                                                                                           ggCCTGATAAGACGCGTAAAGCGTCGCATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTAGTCCGCTCGGCCCAGTCATTCAGGCGTCGTTTCGCTTCGt                            >  1:258731/2‑150 (MQ=255)
                                                                                                                             ccTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTcc                          <  2:862441/150‑1 (MQ=255)
                                                                                                                             ccTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCCGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTcc                          <  2:828020/150‑1 (MQ=255)
                                                                                                                              cTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTCCGGGAATCTTTATTCCGCTCGGCCAAGTCATTCAGGCGACGTTTCGCTTCGTCCt                         >  2:1114092/1‑150 (MQ=255)
                                                                                                                                  tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAg                     <  2:1336118/150‑1 (MQ=255)
                                                                                                                                    cGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGCCGTTTCGCTTCGTCCTGTAGAt                   >  2:790833/1‑150 (MQ=255)
                                                                                                                                     gACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATg                  <  2:1264698/150‑1 (MQ=255)
                                                                                                                                         ctatACGCGTCTTATCAGGCCTACAGGACTGGGCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCGCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTg              <  2:317041/147‑1 (MQ=255)
                                                                                                                                          tttACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTGCGCTTCGTCCTGTAGATGTTTgg             >  2:1979550/1‑149 (MQ=255)
                                                                                                                                            tACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc           <  2:1743172/150‑1 (MQ=255)
                                                                                                                                            tACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc           <  1:611145/150‑1 (MQ=255)
                                                                                                                                            tACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc           <  2:952583/150‑1 (MQ=255)
                                                                                                                                             aCGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCa          >  2:1674356/1‑150 (MQ=255)
                                                                                                                                                cgTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTa       <  1:232666/150‑1 (MQ=255)
                                                                                                                                                   cTTATCAGGCCAACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACt    <  2:1314902/150‑1 (MQ=255)
                                                                                                                                                    ttATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTg   >  2:173365/1‑150 (MQ=255)
                                                                                                                                                     tATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGa  >  2:551715/1‑150 (MQ=255)
                                                                                                                                                     tATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGa  >  1:2065991/1‑150 (MQ=255)
                                                                                                                                                     tATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGa  <  1:2123744/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGTGGAGATTGAGCCACCCTGGCACGTTCACCGGCTTGAGCGTTCCATAGTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGA  >  NC_000913/2139578‑2139876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: