Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,151 C→T 5.3% pseudogene (212/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,1510CT 5.3% 429.1 / 8.2 133pseudogene (212/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (68/57);  new base T (3/4);  total (72/61)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 137 total aligned reads displayed.

TGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTA  >  NC_000913/2173002‑2173299
                                                                                                                                                     |                                                                                                                                                    
tGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATTGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAcc                                                                                                                                                      <  1:357875/150‑1 (MQ=255)
tGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAcc                                                                                                                                                      >  2:1805112/1‑150 (MQ=255)
tGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAcc                                                                                                                                                      <  1:1832384/150‑1 (MQ=255)
   aCCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaaa                                                                                                                                                   >  1:1307333/1‑149 (MQ=255)
     cGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAg                                                                                                                                                 <  2:202281/150‑1 (MQ=255)
      tataataATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGt                                                                                                                                                <  2:1706826/149‑1 (MQ=255)
      gATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGt                                                                                                                                                >  1:1161293/1‑150 (MQ=255)
       ataataATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGtt                                                                                                                                               >  1:640100/1‑150 (MQ=255)
        taataaTGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACACCCGGCTTTTAACGCCCCAGCATTAGACGCCAGTTGGGTGTGCAAACCAATAGttt                                                                                                                                              >  1:1399302/1‑150 (MQ=255)
         aataatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTg                                                                                                                                             <  1:1031383/150‑1 (MQ=255)
            aatGAACACGGGAATATATTGAGGGGAGAAAACCTGAATCACTAACCAGGTAATGGGAGAACCGTCCTTATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCTGTTGTGTGTGCAAACTATTGGTTTGcac                                                                                                                                          <  2:270534/150‑3 (MQ=255)
            aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGt                                                                                                                                          >  2:1200952/1‑150 (MQ=255)
             atGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCACCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTc                                                                                                                                         >  1:1425181/1‑150 (MQ=255)
             atGAAACCGGGAATAGTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCggc                                                                                                                                         >  1:1361943/1‑148 (MQ=255)
              tGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcacac                                                                                                                                        <  2:301099/150‑5 (MQ=255)
              tGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcacac                                                                                                                                        <  1:706792/150‑5 (MQ=255)
               gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCGTACCCCCGGCTTTTTCCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                       >  1:444743/1‑150 (MQ=255)
               gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                       <  1:1600645/150‑1 (MQ=255)
               gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                       <  2:452404/150‑1 (MQ=255)
                   ccGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCATGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATc                                                                                                                                   <  2:1183750/150‑1 (MQ=255)
                   ccGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATc                                                                                                                                   >  2:2029052/1‑150 (MQ=255)
                   ccGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATAACCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATc                                                                                                                                   >  1:1548363/1‑150 (MQ=255)
                    cGGGAATATTTTGCGGTGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                  <  2:1315060/150‑1 (MQ=255)
                    cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                  <  1:1694085/150‑1 (MQ=255)
                       gAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCTGCATTTAACGCCCCAGCATAAGCCGCCACTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATAcaca                                                                                                                               >  2:1373724/1‑150 (MQ=255)
                       gAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCcaca                                                                                                                               >  1:1455322/1‑150 (MQ=255)
                            ttttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTg                                                                                                                          >  1:601598/1‑150 (MQ=255)
                             tttGCGGGGAGAAAAACTGAATCAGTAACCAGGTAATGGGAGAACCGCCATGATACATTGAAGACACAAAACCCCCGGCTTTTAACGCCCCAGCATTAGCCCACAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGa                                                                                                                         >  1:924473/1‑150 (MQ=255)
                               tGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATg                                                                                                                       <  2:188218/150‑1 (MQ=255)
                                 cGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCt                                                                                                                     >  2:1730603/1‑150 (MQ=255)
                                 cGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCt                                                                                                                     <  2:2052692/150‑1 (MQ=255)
                                  gggtAGAAAACCTGAATAAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGGCGCGATCCACAGGGTGATGCTc                                                                                                                    <  2:443759/150‑1 (MQ=255)
                                  ggggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTc                                                                                                                    <  2:1571839/150‑1 (MQ=255)
                                       gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAat                                                                                                               >  2:694791/1‑150 (MQ=255)
                                       gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAat                                                                                                               >  2:1832526/1‑150 (MQ=255)
                                         aaaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatga                                                                                                             <  2:1533743/150‑1 (MQ=255)
                                          aaCCTGAATAAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGTGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgat                                                                                                            <  1:1120659/150‑1 (MQ=255)
                                           aCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgatg                                                                                                           <  2:1455322/150‑1 (MQ=255)
                                            ccTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCTATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAAtgatga                                                                                                          >  2:1814566/1‑150 (MQ=255)
                                             cTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGac                                                                                                         <  2:1907810/150‑1 (MQ=255)
                                                  tCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCg                                                                                                    >  2:1642597/1‑150 (MQ=255)
                                                    aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCgag                                                                                                  >  2:228658/1‑150 (MQ=255)
                                                         ccAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTaa                                                                                             >  1:507394/1‑150 (MQ=255)
                                                           aGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAgg                                                                                           <  1:1469898/150‑1 (MQ=255)
                                                             gTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTg                                                                                         <  2:772738/150‑1 (MQ=255)
                                                               aaTGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGGCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCg                                                                                       <  2:1447403/150‑1 (MQ=255)
                                                                aTGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCgg                                                                                      <  2:49386/150‑1 (MQ=255)
                                                                 tGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGCTCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGa                                                                                     >  2:1214194/1‑150 (MQ=255)
                                                                 tGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGa                                                                                     >  2:1962084/1‑150 (MQ=255)
                                                                      gaACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGa                                                                                <  1:223099/150‑1 (MQ=255)
                                                                        aCCGCGCTGATCCAGTGAAGCCACCCTTCCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTATGGTGCGGAACAGAtt                                                                              <  1:1922139/150‑1 (MQ=255)
                                                                         ccGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATTATGACACCCGAGAGTATGGTGCGGAACAGAttt                                                                             >  2:1331647/1‑150 (MQ=255)
                                                                            cccTGATCCATTGAAGCCCCCATACCACGGCTTTTAACGCCCCAGCATAAGCCGCCAGTTGGGTGTGCAAACCAGTACTTTGAGTCTCGATCCACATGGTGATGATCAAAATGATGACCCCCGAGATTAAGCTGCTGAACAGATTTCCAc                                                                         >  2:1961820/1‑150 (MQ=255)
                                                                             ccTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCAc                                                                         >  1:826861/1‑150 (MQ=255)
                                                                               tGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGa                                                                       <  1:1597515/150‑1 (MQ=255)
                                                                               tGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGa                                                                       >  2:653939/1‑150 (MQ=255)
                                                                                     aTTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACg                                                                 >  2:64273/1‑150 (MQ=255)
                                                                                        gAAGCCCCCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCg                                                              >  2:1379640/1‑150 (MQ=255)
                                                                                          aGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGACGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGAc                                                            >  1:1464094/1‑150 (MQ=255)
                                                                                           gCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGCTGCACGGCGccc                                                           >  1:186394/1‑150 (MQ=255)
                                                                                            ccaccaTACCCCCGGCTTTTAACGCCCCAGCATTCGCCGCCAGTTGGGGGTGCAAACCAATAGTTTGCGGCGCGATCCACAGGGTGATGCGCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCg                                                          <  1:182188/150‑1 (MQ=255)
                                                                                                  tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                    <  1:340868/150‑1 (MQ=255)
                                                                                                  tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                    <  1:525621/150‑1 (MQ=255)
                                                                                                       ccGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCATTAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGCCACCCGAGATTAAGGTGCGGAACAGATTACCACGATGCACGGCGACTGCCCTCGCCACg                                               >  1:2211773/1‑150 (MQ=255)
                                                                                                       ccGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGGCACg                                               >  2:162297/1‑150 (MQ=255)
                                                                                                           cTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaaga                                           >  1:998478/1‑150 (MQ=255)
                                                                                                            ttttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaagaa                                          >  2:1024163/1‑150 (MQ=255)
                                                                                                            ttttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaagaa                                          >  1:705335/1‑150 (MQ=255)
                                                                                                            ttttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaagaa                                          >  1:1473867/1‑150 (MQ=255)
                                                                                                             tttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACTGATTTACAGTATGCACGTCGACCGCGATCGACACGaagaag                                         <  2:2118676/150‑1 (MQ=255)
                                                                                                              ttACCGCCCCAGCATTAGCCGCCAGAGGGGGGGGCATACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTAATACTGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGc                                        <  2:1631934/150‑1 (MQ=255)
                                                                                                              ttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCCCAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGc                                        >  1:2115019/1‑150 (MQ=255)
                                                                                                               tAAGGCCCCAGCATTAGCCGCCAGTTGGGTGTGGAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGcc                                       <  2:1394229/150‑1 (MQ=255)
                                                                                                               tAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGcc                                       <  1:110705/150‑1 (MQ=255)
                                                                                                               tAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGcc                                       >  1:766950/1‑150 (MQ=255)
                                                                                                                aaCGCCCCAGCATTAGCCGCCAGGTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGCGGCTCATAATGAGGACCCCCGAGCTTAAGGTGCGGACCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGACGCCg                                      >  2:516335/1‑150 (MQ=255)
                                                                                                                aaCGCCCCAGCAATAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGTTCCACAGGGAGCTGCACATAATGATGAAGCCGCAGATTAAGGTGCGCAAGAGATTTCCACCATGCACGCCGACCGCCATCGCGACGAAGAAGccc                                      >  2:516337/1‑149 (MQ=255)
                                                                                                                    ccccAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGATCCCCAGGGTGATGCTCATAAGGATTAAACACGAGATTAAGGTGCGGATCATATTTCCACGATGCACGGCGACCGCCATCGACAAGAAGAAGACGAtgg                                  >  2:361562/1‑150 (MQ=255)
                                                                                                                       cAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTTATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAtggtgg                               <  2:329443/150‑1 (MQ=255)
                                                                                                                       cAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAtggtgg                               <  1:1331727/150‑1 (MQ=255)
                                                                                                                        aGCATTAGCCGCCAGTGGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGc                              <  2:1972103/150‑1 (MQ=255)
                                                                                                                          cATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAAAGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCaa                            >  2:1002980/1‑150 (MQ=255)
                                                                                                                             tAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGAt                         >  1:477558/1‑150 (MQ=255)
                                                                                                                                     aGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAc                 <  1:533975/150‑1 (MQ=255)
                                                                                                                                        tGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGCAGCCGATGGTGGCAACATCGCCAAACGgc              >  1:366830/1‑150 (MQ=255)
                                                                                                                                        tGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgc              <  1:2029052/150‑1 (MQ=255)
                                                                                                                                        tGGGGGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgc              <  2:261250/150‑1 (MQ=255)
                                                                                                                                         ggttgtgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTAATAATGATGACACCCGAGATTAAGGTGCGTAACAGATTACCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgca             <  2:808495/147‑1 (MQ=255)
                                                                                                                                         gggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgca             >  1:1074146/1‑150 (MQ=255)
                                                                                                                                          ggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcag            <  1:1730603/150‑1 (MQ=255)
                                                                                                                                          ggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcag            >  1:1541382/1‑150 (MQ=255)
                                                                                                                                            ggtgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagca          <  2:2020067/149‑1 (MQ=255)
                                                                                                                                            gacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagca          >  1:222283/4‑150 (MQ=255)
                                                                                                                                             gtgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCAc         >  1:1666593/1‑150 (MQ=255)
                                                                                                                                             acgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCAc         >  2:1467412/3‑150 (MQ=255)
                                                                                                                                                cAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCCCCTg      >  1:581016/1‑150 (MQ=255)
                                                                                                                                                cAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGAGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGGAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTg      >  1:1146974/1‑150 (MQ=255)
                                                                                                                                                 aaaCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGGGCGGAACAGATTTCCACTATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGa     <  1:1820192/150‑1 (MQ=255)
                                                                                                                                                  aaCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACACGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAt    >  2:1746562/1‑150 (MQ=255)
                                                                                                                                                    ccAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTa  >  1:1881752/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTA  >  NC_000913/2173002‑2173299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: