Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,153 A→T 6.8% pseudogene (210/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,1530AT 6.8% 393.0 / 10.6 133pseudogene (210/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base A (66/57);  new base T (5/4);  total (72/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

Only 100 of 135 total aligned reads displayed.

ACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCC  >  NC_000913/2173005‑2173302
                                                                                                                                                    |                                                                                                                                                     
aCCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaaa                                                                                                                                                      >  1:1307333/1‑149 (MQ=255)
   gATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGt                                                                                                                                                   >  1:1161293/1‑150 (MQ=255)
    ataataATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGtt                                                                                                                                                  >  1:640100/1‑150 (MQ=255)
     taataaTGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACACCCGGCTTTTAACGCCCCAGCATTAGACGCCAGTTGGGTGTGCAAACCAATAGttt                                                                                                                                                 >  1:1399302/1‑150 (MQ=255)
      aataatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTg                                                                                                                                                <  1:1031383/150‑1 (MQ=255)
         aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcac                                                                                                                                             <  2:404085/150‑3 (MQ=255)
         aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGt                                                                                                                                             >  2:1200952/1‑150 (MQ=255)
          atGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTc                                                                                                                                            >  1:1447403/1‑150 (MQ=255)
          atGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCACCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTc                                                                                                                                            >  1:1425181/1‑150 (MQ=255)
          atGAAACCGGGAATAGTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCggc                                                                                                                                            >  1:1361943/1‑148 (MQ=255)
           tGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcacac                                                                                                                                           <  1:706792/150‑5 (MQ=255)
           tGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcacac                                                                                                                                           <  2:301099/150‑5 (MQ=255)
            gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCGTACCCCCGGCTTTTTCCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                          >  1:444743/1‑150 (MQ=255)
            gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                          <  2:452404/150‑1 (MQ=255)
            gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                          <  1:1600645/150‑1 (MQ=255)
               aCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGAt                                                                                                                                       <  2:1589523/150‑1 (MQ=255)
                ccGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCATGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATc                                                                                                                                      <  2:1183750/150‑1 (MQ=255)
                ccGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATc                                                                                                                                      >  2:2029052/1‑150 (MQ=255)
                ccGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATAACCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATc                                                                                                                                      >  1:1548363/1‑150 (MQ=255)
                 cGGGAATATTTTGCGGTGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                     <  2:1315060/150‑1 (MQ=255)
                 cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                     <  1:1694085/150‑1 (MQ=255)
                  gggAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTACCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCca                                                                                                                                    >  2:1331727/1‑150 (MQ=255)
                    gAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGAGCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCcaca                                                                                                                                  <  1:1592100/150‑1 (MQ=255)
                         ttttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTg                                                                                                                             >  1:601598/1‑150 (MQ=255)
                          tttGCGGGGAGAAAAACTGAATCAGTAACCAGGTAATGGGAGAACCGCCATGATACATTGAAGACACAAAACCCCCGGCTTTTAACGCCCCAGCATTAGCCCACAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGa                                                                                                                            >  1:924473/1‑150 (MQ=255)
                            tGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATg                                                                                                                          <  2:188218/150‑1 (MQ=255)
                              cGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCt                                                                                                                        >  2:1730603/1‑150 (MQ=255)
                              cGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCt                                                                                                                        <  2:2052692/150‑1 (MQ=255)
                               gggtAGAAAACCTGAATAAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGGCGCGATCCACAGGGTGATGCTc                                                                                                                       <  2:443759/150‑1 (MQ=255)
                               ggggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTc                                                                                                                       <  2:1571839/150‑1 (MQ=255)
                                gggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCa                                                                                                                      <  1:1790055/150‑1 (MQ=255)
                                    gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAat                                                                                                                  >  2:694791/1‑150 (MQ=255)
                                    gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAat                                                                                                                  >  2:1832526/1‑150 (MQ=255)
                                      aaaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatga                                                                                                                <  2:1533743/150‑1 (MQ=255)
                                       aaCCTGAATAAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGTGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgat                                                                                                               <  1:1120659/150‑1 (MQ=255)
                                        aCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgatg                                                                                                              <  2:1455322/150‑1 (MQ=255)
                                        aCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgatg                                                                                                              >  1:2155482/1‑150 (MQ=255)
                                            gAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCTGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGacac                                                                                                          <  2:1830300/150‑1 (MQ=255)
                                               tCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCg                                                                                                       >  2:1642597/1‑150 (MQ=255)
                                                 aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCgag                                                                                                     >  2:228658/1‑150 (MQ=255)
                                                        aGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAgg                                                                                              <  1:1469898/150‑1 (MQ=255)
                                                          gTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTg                                                                                            <  2:772738/150‑1 (MQ=255)
                                                           tAATGGGAGAACCGCCCGGAGCCATTGAAGCCACAATACCCCCGGCTTTTAACGCCCCAGAATTTGCCGCCAGTTGGTTGTGAAAACAATTAGTTTGCGTCGCGAACCCCAGGGTGATGGACATATGGAGGACCCCCGAGAGTACGGTGc                                                                                           >  2:298527/1‑150 (MQ=255)
                                                            aaTGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGGCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCg                                                                                          <  2:1447403/150‑1 (MQ=255)
                                                             aTGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCgg                                                                                         <  2:49386/150‑1 (MQ=255)
                                                              tGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGCTCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGa                                                                                        >  2:1214194/1‑150 (MQ=255)
                                                              tGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGa                                                                                        >  2:1962084/1‑150 (MQ=255)
                                                                 gagaACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACa                                                                                     >  2:1651045/1‑150 (MQ=255)
                                                                   gaACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGa                                                                                   >  2:195697/1‑150 (MQ=255)
                                                                     aCCGCGCTGATCCAGTGAAGCCACCCTTCCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTATGGTGCGGAACAGAtt                                                                                 <  1:1922139/150‑1 (MQ=255)
                                                                      ccGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAACCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGAttt                                                                                >  1:1611365/1‑150 (MQ=255)
                                                                      ccGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATTATGACACCCGAGAGTATGGTGCGGAACAGAttt                                                                                >  2:1331647/1‑150 (MQ=255)
                                                                         cccTGATCCATTGAAGCCCCCATACCACGGCTTTTAACGCCCCAGCATAAGCCGCCAGTTGGGTGTGCAAACCAGTACTTTGAGTCTCGATCCACATGGTGATGATCAAAATGATGACCCCCGAGATTAAGCTGCTGAACAGATTTCCAc                                                                            >  2:1961820/1‑150 (MQ=255)
                                                                          ccTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCAc                                                                            >  1:826861/1‑150 (MQ=255)
                                                                            tGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGa                                                                          >  2:653939/1‑150 (MQ=255)
                                                                                  aTTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACg                                                                    >  2:64273/1‑150 (MQ=255)
                                                                                     gAAGCCCCCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCg                                                                 >  2:1379640/1‑150 (MQ=255)
                                                                                       aGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGACGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGAc                                                               >  1:1464094/1‑150 (MQ=255)
                                                                                        gCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGCTGCACGGCGccc                                                              >  1:186394/1‑150 (MQ=255)
                                                                                         ccaccaTACCCCCGGCTTTTAACGCCCCAGCATTCGCCGCCAGTTGGGGGTGCAAACCAATAGTTTGCGGCGCGATCCACAGGGTGATGCGCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCg                                                             <  1:182188/150‑1 (MQ=255)
                                                                                         ccaccaTACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCg                                                             <  1:1832526/150‑1 (MQ=255)
                                                                                               tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                       <  1:525621/150‑1 (MQ=255)
                                                                                               tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                       <  1:340868/150‑1 (MQ=255)
                                                                                                  ccccGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGccc                                                    >  2:2050006/1‑149 (MQ=255)
                                                                                                    ccGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCATTAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGCCACCCGAGATTAAGGTGCGGAACAGATTACCACGATGCACGGCGACTGCCCTCGCCACg                                                  >  1:2211773/1‑150 (MQ=255)
                                                                                                        cTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaaga                                              >  1:998478/1‑150 (MQ=255)
                                                                                                         ttttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaagaa                                             >  1:705335/1‑150 (MQ=255)
                                                                                                         ttttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaagaa                                             >  2:1024163/1‑150 (MQ=255)
                                                                                                           ttACCGCCCCAGCATTAGCCGCCAGAGGGGGGGGCATACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTAATACTGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGc                                           <  2:1631934/150‑1 (MQ=255)
                                                                                                            tAAGGCCCCAGCATTAGCCGCCAGTTGGGTGTGGAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGcc                                          <  2:1394229/150‑1 (MQ=255)
                                                                                                            tAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGcc                                          <  1:110705/150‑1 (MQ=255)
                                                                                                            tAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGcc                                          >  1:766950/1‑150 (MQ=255)
                                                                                                             aaCGCCCCAGCATTAGCCGCCAGGTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGCGGCTCATAATGAGGACCCCCGAGCTTAAGGTGCGGACCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGACGCCg                                         >  2:516335/1‑150 (MQ=255)
                                                                                                             aaCGCCCCAGCAATAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGTTCCACAGGGAGCTGCACATAATGATGAAGCCGCAGATTAAGGTGCGCAAGAGATTTCCACCATGCACGCCGACCGCCATCGCGACGAAGAAGccc                                         >  2:516337/1‑149 (MQ=255)
                                                                                                               cGCCCCAGCATTAGCCGCCAGTTGGGTGAGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTTAGGTGCGGACCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAt                                       >  2:1999522/1‑150 (MQ=255)
                                                                                                                    cAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTTATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAtggtgg                                  <  2:329443/150‑1 (MQ=255)
                                                                                                                    cAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAtggtgg                                  <  1:1331727/150‑1 (MQ=255)
                                                                                                                     aGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGc                                 <  2:2155482/150‑1 (MQ=255)
                                                                                                                       cATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAAAGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCaa                               >  2:1002980/1‑150 (MQ=255)
                                                                                                                          tAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGAt                            >  1:477558/1‑150 (MQ=255)
                                                                                                                                  aGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAc                    <  1:533975/150‑1 (MQ=255)
                                                                                                                                     tGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGCAGCCGATGGTGGCAACATCGCCAAACGgc                 >  1:366830/1‑150 (MQ=255)
                                                                                                                                     tGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgc                 <  1:2029052/150‑1 (MQ=255)
                                                                                                                                     tGGGGGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgc                 <  2:261250/150‑1 (MQ=255)
                                                                                                                                      ggttgtgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTAATAATGATGACACCCGAGATTAAGGTGCGTAACAGATTACCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgca                <  2:808495/147‑1 (MQ=255)
                                                                                                                                      gggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgca                <  1:491420/150‑1 (MQ=255)
                                                                                                                                      gggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgca                <  1:1931937/150‑1 (MQ=255)
                                                                                                                                      gggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgca                >  1:1074146/1‑150 (MQ=255)
                                                                                                                                       ggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcag               <  1:1730603/150‑1 (MQ=255)
                                                                                                                                       ggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcag               >  1:1541382/1‑150 (MQ=255)
                                                                                                                                       ggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAAGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcag               >  2:1793279/1‑150 (MQ=255)
                                                                                                                                         ggtgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagca             <  2:2020067/149‑1 (MQ=255)
                                                                                                                                         gacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagca             >  1:222283/4‑150 (MQ=255)
                                                                                                                                          gtgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCAc            >  1:28692/1‑150 (MQ=255)
                                                                                                                                          acgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCAc            >  2:1467412/3‑150 (MQ=255)
                                                                                                                                             cAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCCCCTg         >  1:581016/1‑150 (MQ=255)
                                                                                                                                             cAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGAGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGGAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTg         >  1:1146974/1‑150 (MQ=255)
                                                                                                                                               aaCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACACGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAt       >  2:1746562/1‑150 (MQ=255)
                                                                                                                                                aCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAtt      >  1:1470107/1‑150 (MQ=255)
                                                                                                                                                    aTAGTTTGCGTCGCGATCCACAGGGGGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTAccc  <  1:708496/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCC  >  NC_000913/2173005‑2173302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: