Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,230,416 G→C 5.8% intergenic (‑31/‑208) dusC ← / → yohJ tRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,230,4160GC 5.8% 490.2 / 14.4 151intergenic (‑31/‑208)dusC/yohJtRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (86/55);  new base C (5/4);  total (92/59)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

AGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATC  >  NC_000913/2230267‑2230565
                                                                                                                                                     |                                                                                                                                                     
agTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATg                                                                                                                                                       >  1:1953184/1‑150 (MQ=255)
agTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATg                                                                                                                                                       >  2:1773178/1‑150 (MQ=255)
 gTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGt                                                                                                                                                      >  1:2129458/1‑150 (MQ=255)
 gTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGt                                                                                                                                                      >  1:774824/1‑150 (MQ=255)
   tGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTa                                                                                                                                                    >  2:1546500/1‑150 (MQ=255)
    gaaccaccaCGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAg                                                                                                                                                   >  1:1382038/4‑150 (MQ=255)
    gATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTat                                                                                                                                                   >  2:729081/1‑149 (MQ=255)
    gATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAg                                                                                                                                                   >  2:2163264/1‑150 (MQ=255)
     aTCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACCCGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAgc                                                                                                                                                  >  1:1634320/1‑150 (MQ=255)
        caccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGGCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCAcc                                                                                                                                               <  2:1095743/150‑1 (MQ=255)
                       cTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGc                                                                                                                                     >  2:1325810/1‑145 (MQ=255)
                       cTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGc                                                                                                                                     <  1:1325810/145‑1 (MQ=255)
                       cTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAtt                                                                                                                                >  1:940281/1‑150 (MQ=255)
                         cGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCTGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTaa                                                                                                                              >  1:990702/1‑150 (MQ=255)
                             gATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGt                                                                                                                          >  2:508681/1‑150 (MQ=255)
                                gCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAATCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTg                                                                                                                       <  1:608350/150‑1 (MQ=255)
                                 cacaGATCGTAGTCGTTAACTTCGGTCCGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTATCGCCTATGGTCAGCGGATTAAAGGTCTGc                                                                                                                      >  2:2002583/1‑150 (MQ=255)
                                 cacaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGc                                                                                                                      >  1:1760160/1‑150 (MQ=255)
                                    aGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaaa                                                                                                                   >  1:1768752/1‑150 (MQ=255)
                                      aTCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAaga                                                                                                                 <  1:913870/150‑1 (MQ=255)
                                         gTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                                                              >  1:2194867/1‑150 (MQ=255)
                                            gTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGAtt                                                                                                           >  1:1632231/1‑150 (MQ=255)
                                             tCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtg                                                                                                          <  2:1186576/150‑1 (MQ=255)
                                              cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                                         <  1:475646/150‑1 (MQ=255)
                                              cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                                         >  1:1111330/1‑150 (MQ=255)
                                              cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                                         >  1:1959926/1‑150 (MQ=255)
                                               gTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                                                                        >  2:2079753/1‑150 (MQ=255)
                                               gTTAACATCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGTCGCTATGTTAGCGCCTATGGACAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                                                                        >  1:118785/1‑150 (MQ=255)
                                                    cTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                                   >  2:879001/1‑150 (MQ=255)
                                                        ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGc                                                                                               <  1:1729588/150‑1 (MQ=255)
                                                        ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGc                                                                                               >  1:220181/1‑150 (MQ=255)
                                                        ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGc                                                                                               <  2:1797645/150‑1 (MQ=255)
                                                        ggTCAGCAATTCACGCACAAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATAACCCGCAAAAAAATGAGGCGCTATCTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGc                                                                                               >  1:1310306/1‑150 (MQ=255)
                                                           cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAGGTTAGCGCCTATGGTCAGCGGATTAAAGGGCTGCAAAGAGGGATTGCTGCCAACTGCCAt                                                                                            >  1:1901960/1‑150 (MQ=255)
                                                             gCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                          >  2:2097564/1‑150 (MQ=255)
                                                             gCAATTAACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                          >  2:54481/1‑150 (MQ=255)
                                                               aaTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCct                                                                                        >  2:1093297/1‑150 (MQ=255)
                                                                aTTCACGCACCAGAGAGTCAAGCACTCCATCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCATATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctc                                                                                       <  1:444784/150‑1 (MQ=255)
                                                                 ttCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCAGTTTGCAGCAATCCctct                                                                                      <  1:1817518/150‑1 (MQ=255)
                                                                  tCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctc                                                                                     <  1:1780920/150‑1 (MQ=255)
                                                                  tCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctc                                                                                     <  2:1662217/150‑1 (MQ=255)
                                                                   cacacaCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctct                                                                                    >  2:591542/1‑150 (MQ=255)
                                                                    ccGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGTCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTa                                                                                   <  1:1484315/149‑1 (MQ=255)
                                                                         ccAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCtt                                                                              <  2:291296/150‑1 (MQ=255)
                                                                         ccAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCtt                                                                              >  1:558230/1‑150 (MQ=255)
                                                                         ccAGAGAGTCAAGCACTCCCTCCATCGGTGCAAGTTACACACGGATATCATCACCCACAAAAAAATCAGCCGCTATGTTAGCCCCTATGCTCTGCGGATTAAAAGTAATCAAAGAGGGATTTCTGCAAAATGCAATTCCTCTCTATGGtt                                                                              >  1:347825/1‑150 (MQ=255)
                                                                          cAGAGAGTCAAGCACTCCCTCCATCTGTGGCAGTAACACACGCATTTCATCACCCGCAAAAAAATTAGTCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTg                                                                             <  2:2098355/150‑1 (MQ=255)
                                                                          cAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTg                                                                             >  2:190791/1‑150 (MQ=255)
                                                                             agagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATg                                                                          <  2:2042136/150‑1 (MQ=255)
                                                                                gTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAACCCTCTCTATCCTTGATGTAt                                                                       >  1:297172/1‑150 (MQ=255)
                                                                                gTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCCATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTAt                                                                       >  1:2085231/1‑150 (MQ=255)
                                                                                  cAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATAACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGc                                                                     >  1:100557/1‑150 (MQ=255)
                                                                                       aCTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAGACTGCAATCCCTCTCTATGCTTGATGTATGCCTGat                                                                >  2:1735042/1‑149 (MQ=255)
                                                                                       aCTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAg                                                                >  1:583721/1‑150 (MQ=255)
                                                                                       aCTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCGCTCTATGCTTGATGTATGCCTGAg                                                                >  1:1611808/1‑150 (MQ=255)
                                                                                        ctccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGt                                                               >  1:1915714/1‑150 (MQ=255)
                                                                                            ctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                                                           >  1:307807/1‑150 (MQ=255)
                                                                                            atccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCTTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                                                           <  1:729081/149‑1 (MQ=255)
                                                                                               cATCGGTGCCACTACCACACGCATATCATCACACGCAAAAAAATGAGGCGCTATTTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATAGCTGACAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGCGTTCGCTgc                                                        >  2:355404/1‑150 (MQ=255)
                                                                                                 tCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcgc                                                      <  1:1546500/150‑1 (MQ=255)
                                                                                                    gTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCAtt                                                   >  1:1004886/1‑150 (MQ=255)
                                                                                                      gCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGc                                                 <  1:537764/150‑1 (MQ=255)
                                                                                                       ccAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCg                                                >  2:2140898/1‑150 (MQ=255)
                                                                                                            aaCACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATg                                           >  1:1484887/1‑150 (MQ=255)
                                                                                                            aaCACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATg                                           >  2:225439/1‑150 (MQ=255)
                                                                                                              cacacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTTAAGGTCTGCAAAGAGGGATTGCTGCAAACTGAAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGcc                                         >  2:92054/1‑150 (MQ=255)
                                                                                                              cacacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGcc                                         >  2:1832199/1‑150 (MQ=255)
                                                                                                                cacGCATATCATCGCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTAAGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCgg                                       >  1:527547/1‑150 (MQ=255)
                                                                                                                cacGCATATCATCACCCGCAAATAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAATCTGCACACCCTCTCTAACCTAGATGTATGCCTGAGTTCGCTGCGCATTGCGGATTGCCgg                                       >  2:784336/1‑150 (MQ=255)
                                                                                                                cacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCgg                                       >  1:392383/1‑150 (MQ=255)
                                                                                                                    cATATCATCACCCGAAAATAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGATGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAg                                   <  2:1144347/150‑1 (MQ=255)
                                                                                                                       atcatcACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGa                                >  2:1849780/1‑150 (MQ=255)
                                                                                                                         catcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCCAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaa                              >  1:56725/1‑150 (MQ=255)
                                                                                                                         catcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaa                              >  2:1952705/1‑150 (MQ=255)
                                                                                                                          atcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaa                             >  1:349572/1‑150 (MQ=255)
                                                                                                                            caCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaaaa                           <  1:602057/150‑1 (MQ=255)
                                                                                                                             aCCCGCAAAAATATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAAt                          <  2:640963/150‑1 (MQ=255)
                                                                                                                              cccGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATa                         >  2:1857223/1‑150 (MQ=255)
                                                                                                                                  cAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgcg                     >  1:1814779/1‑150 (MQ=255)
                                                                                                                                   aaaaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgcgc                    >  1:2048631/1‑150 (MQ=255)
                                                                                                                                    aaaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCt                   <  1:1927499/150‑1 (MQ=255)
                                                                                                                                     aaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCGCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAACAAATAAGCGCTg                  >  1:2031771/1‑150 (MQ=255)
                                                                                                                                      aaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGa                 >  1:488018/1‑150 (MQ=255)
                                                                                                                                      aaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATAACTCTCTATGCTTGATGGATGCCTGAGTTAGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGa                 >  1:1277115/1‑150 (MQ=255)
                                                                                                                                         cataGGCGCTAACATAGCGCCTATGGGCAGCGGATTAAAGGTCTGCAAAGAGGGCTTGCTGCAAACTGCAATCACTCTCTATGCTTGATGTATTCCTTAGTTCGCTGCGCATTGCGTAAAGCCGGGGAGCGAAAAAATATGCGCTGAttt              >  2:356761/4‑150 (MQ=255)
                                                                                                                                          tGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCACAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCTCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTc             <  1:1590968/150‑1 (MQ=255)
                                                                                                                                          tGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGAttta             >  2:468830/1‑149 (MQ=255)
                                                                                                                                          tGAGGCGCTATGCTAGCGCCTATGGTCAGCGGATTAAAGGGCTGCAAAGAGCGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTGCGCTGCGCATTGCGCAATGCCGGGGATCGAAAAAATAAGCGCTGATTTc             <  2:1766993/150‑1 (MQ=255)
                                                                                                                                           taGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt            >  2:418739/2‑150 (MQ=255)
                                                                                                                                           gAGGCTCTATGTTAGCGACTATTGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt            <  2:712800/150‑1 (MQ=255)
                                                                                                                                           gAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGCATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt            <  2:1012690/150‑1 (MQ=255)
                                                                                                                                            aGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt           <  2:1812247/150‑1 (MQ=255)
                                                                                                                                            aGGCGCTATGTTAGCGCCTATGGGCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTTCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt           <  1:10412/150‑1 (MQ=255)
                                                                                                                                              gcgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTaa         <  2:307807/150‑1 (MQ=255)
                                                                                                                                               cgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGTGCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTTTGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTTAt        <  1:1351598/150‑1 (MQ=255)
                                                                                                                                                gcTATGTTAGCGCCTATGGGCAGCGGATTAAAGGGCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtg       <  2:798580/150‑1 (MQ=255)
                                                                                                                                                 ctttGTTAGCGCCTATTGTGAGCGGATTAAAGGTCTGCCAAGAGGGATTGCTGCAAACTGCTATCCCTCTCTATGCTTGATGTCTGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGTGAGCGAAAAAATAAGCGCTGATTTCTTAAtgt      <  2:1389619/147‑1 (MQ=255)
                                                                                                                                                 cTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCTGGGAGCCAAAAAATTAGCGCTGATTTCTTAAtgt      >  1:388863/1‑150 (MQ=255)
                                                                                                                                                    tGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGAt   <  2:1768406/150‑1 (MQ=255)
                                                                                                                                                     gTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATc  >  2:2175435/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATC  >  NC_000913/2230267‑2230565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: