Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,230,417 T→A 6.0% intergenic (‑32/‑207) dusC ← / → yohJ tRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,230,4170TA 6.0% 430.5 / 11.1 150intergenic (‑32/‑207)dusC/yohJtRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (86/54);  new base A (5/4);  total (91/59)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 153 total aligned reads displayed.

GTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCG  >  NC_000913/2230268‑2230566
                                                                                                                                                     |                                                                                                                                                     
gTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGt                                                                                                                                                       >  1:774824/1‑150 (MQ=255)
gTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGt                                                                                                                                                       >  1:2129458/1‑150 (MQ=255)
   gATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTat                                                                                                                                                    >  2:729081/1‑149 (MQ=255)
   gATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAg                                                                                                                                                    >  2:2163264/1‑150 (MQ=255)
   gATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGATAg                                                                                                                                                    >  1:1298019/1‑150 (MQ=255)
       caccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGGCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCAcc                                                                                                                                                <  2:1095743/150‑1 (MQ=255)
          cacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTAAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCACTCGGTGCCAGTAACACCCGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCAAt                                                                                                                                             >  1:390666/1‑150 (MQ=255)
            cgcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATgg                                                                                                                                           >  1:472244/1‑150 (MQ=255)
            cgcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATgg                                                                                                                                           <  2:1889519/150‑1 (MQ=255)
            cgcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCtcatt                                                                                                                                           <  1:632381/150‑5 (MQ=255)
             gcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTCACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCtcattt                                                                                                                                          <  1:1857310/150‑6 (MQ=255)
                  cAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCCCACCCTCCATCGGGGCCAGTAACCCCCGCATATCATCACACGCAAAAAAATGAGGCGCTAGGTTAGCGCCTAGGGGAAGCg                                                                                                                                     >  1:1389619/1‑150 (MQ=255)
                    aaCTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAATGAGGCGCTAACATAGCGCCtc                                                                                                                                              <  2:371558/138‑2 (MQ=255)
                      cTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAtt                                                                                                                                 >  1:1916832/1‑150 (MQ=255)
                      cTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAtt                                                                                                                                 >  1:940281/1‑150 (MQ=255)
                       tCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTa                                                                                                                                <  1:216639/150‑1 (MQ=255)
                            gATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGt                                                                                                                           >  2:508681/1‑150 (MQ=255)
                              tGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                                                         >  1:543581/1‑150 (MQ=255)
                                cacaGATCGTAGTCGTTAACTTCGGTCCGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTATCGCCTATGGTCAGCGGATTAAAGGTCTGc                                                                                                                       >  2:2002583/1‑150 (MQ=255)
                                  caGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCTATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaa                                                                                                                     >  2:1771589/1‑150 (MQ=255)
                                   aGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaaa                                                                                                                    >  1:1768752/1‑150 (MQ=255)
                                    gATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAag                                                                                                                   <  1:958825/150‑1 (MQ=255)
                                        gTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCATATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                                                               >  1:1812247/1‑150 (MQ=255)
                                           gTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGAtt                                                                                                            >  1:1768406/1‑150 (MQ=255)
                                           gTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGAtt                                                                                                            >  1:1632231/1‑150 (MQ=255)
                                            tCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtg                                                                                                           <  2:1186576/150‑1 (MQ=255)
                                             cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                                          >  1:1111330/1‑150 (MQ=255)
                                             cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                                          >  1:1959926/1‑150 (MQ=255)
                                              gTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                                                                         >  2:2079753/1‑150 (MQ=255)
                                              gTTAACATCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGTCGCTATGTTAGCGCCTATGGACAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                                                                         >  1:118785/1‑150 (MQ=255)
                                                  aCTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaa                                                                                                     <  2:1027533/150‑1 (MQ=255)
                                                   cTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                                    >  2:879001/1‑150 (MQ=255)
                                                       ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGc                                                                                                >  1:220181/1‑150 (MQ=255)
                                                       ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGc                                                                                                <  1:1729588/150‑1 (MQ=255)
                                                       ggTCAGCAATTCACGCACAAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATAACCCGCAAAAAAATGAGGCGCTATCTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGc                                                                                                >  1:1310306/1‑150 (MQ=255)
                                                          cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAGGTTAGCGCCTATGGTCAGCGGATTAAAGGGCTGCAAAGAGGGATTGCTGCCAACTGCCAt                                                                                             >  1:1901960/1‑150 (MQ=255)
                                                            gCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                           >  2:2097564/1‑150 (MQ=255)
                                                            gCAATTAACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                           >  2:54481/1‑150 (MQ=255)
                                                               aTTCACGCACCAGAGAGTCAAGCACTCCATCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCATATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctc                                                                                        <  1:444784/150‑1 (MQ=255)
                                                                 tCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctc                                                                                      <  2:1662217/150‑1 (MQ=255)
                                                                  cacacaCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctct                                                                                     >  2:591542/1‑150 (MQ=255)
                                                                   ccGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGTCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTa                                                                                    <  1:1484315/149‑1 (MQ=255)
                                                                   aCGCACCAGAGAGTCAAGCACTACCTCCATCGGTGTCATTATAACACTCATATCATCACACTCAAAAAAATGAGTCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGTGATTGCTGCAAACTGCAATCCCTCTCTa                                                                                    <  1:1336434/150‑1 (MQ=255)
                                                                        ccAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCtt                                                                               <  2:291296/150‑1 (MQ=255)
                                                                        ccAGAGAGTCAAGCACTCCCTCCATCGGTGCAAGTTACACACGGATATCATCACCCACAAAAAAATCAGCCGCTATGTTAGCCCCTATGCTCTGCGGATTAAAAGTAATCAAAGAGGGATTTCTGCAAAATGCAATTCCTCTCTATGGtt                                                                               >  1:347825/1‑150 (MQ=255)
                                                                         cAGAGAGTCAAGCACTCCCTCCATCTGTGGCAGTAACACACGCATTTCATCACCCGCAAAAAAATTAGTCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTg                                                                              <  2:2098355/150‑1 (MQ=255)
                                                                         cAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTg                                                                              >  2:190791/1‑150 (MQ=255)
                                                                            agagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATg                                                                           <  2:2042136/150‑1 (MQ=255)
                                                                               gTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAACCCTCTCTATCCTTGATGTAt                                                                        >  1:297172/1‑150 (MQ=255)
                                                                                 cAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGc                                                                      >  1:604165/1‑150 (MQ=255)
                                                                                 cAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATAACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGc                                                                      >  1:100557/1‑150 (MQ=255)
                                                                                  aaGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGcc                                                                     <  2:1959926/150‑1 (MQ=255)
                                                                                  aaGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGcc                                                                     <  2:1804092/150‑1 (MQ=255)
                                                                                      aCTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCGCTCTATGCTTGATGTATGCCTGAg                                                                 >  1:1611808/1‑150 (MQ=255)
                                                                                           ctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                                                            >  1:307807/1‑150 (MQ=255)
                                                                                             ccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                          >  2:1577515/1‑150 (MQ=255)
                                                                                              cATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgc                                                         >  1:169373/1‑150 (MQ=255)
                                                                                              cATCGGTGCCACTACCACACGCATATCATCACACGCAAAAAAATGAGGCGCTATTTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATAGCTGACAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGCGTTCGCTgc                                                         >  2:355404/1‑150 (MQ=255)
                                                                                                tCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcgc                                                       <  2:1265529/150‑1 (MQ=255)
                                                                                                tCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcgc                                                       <  1:1546500/150‑1 (MQ=255)
                                                                                                   gTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCAtt                                                    >  1:1004886/1‑150 (MQ=255)
                                                                                                     gCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGc                                                  <  1:537764/150‑1 (MQ=255)
                                                                                                      ccAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCg                                                 >  2:2140898/1‑150 (MQ=255)
                                                                                                        aGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTa                                               >  2:1099147/1‑150 (MQ=255)
                                                                                                           aaCACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATg                                            >  2:225439/1‑150 (MQ=255)
                                                                                                             cacacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTTAAGGTCTGCAAAGAGGGATTGCTGCAAACTGAAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGcc                                          >  2:92054/1‑150 (MQ=255)
                                                                                                               cacGCATATCATCACCCGCAAATAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAATCTGCACACCCTCTCTAACCTAGATGTATGCCTGAGTTCGCTGCGCATTGCGGATTGCCgg                                        >  2:784336/1‑150 (MQ=255)
                                                                                                               cacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCgg                                        >  1:2008164/1‑150 (MQ=255)
                                                                                                                      atcatcACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGa                                 >  2:1849780/1‑150 (MQ=255)
                                                                                                                        catcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCCAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaa                               >  1:56725/1‑150 (MQ=255)
                                                                                                                         atcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaa                              >  1:349572/1‑150 (MQ=255)
                                                                                                                          tcccccGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAGTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTACTGCCGGGGAGCGaaaaa                             >  1:582468/4‑150 (MQ=255)
                                                                                                                          tcaCCCGCAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaaaa                            >  1:151220/1‑150 (MQ=255)
                                                                                                                           caCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaaaa                            <  1:602057/150‑1 (MQ=255)
                                                                                                                            aCCCGCAAAAATATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAAt                           <  2:640963/150‑1 (MQ=255)
                                                                                                                             cccGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTTTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATa                          <  2:316022/150‑1 (MQ=255)
                                                                                                                                 cAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgcg                      >  1:1814779/1‑150 (MQ=255)
                                                                                                                                 cAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgcg                      <  2:1764548/150‑1 (MQ=255)
                                                                                                                                  aaaaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgcgc                     >  1:2048631/1‑150 (MQ=255)
                                                                                                                                   aaaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCt                    >  1:7950/1‑150 (MQ=255)
                                                                                                                                   aaaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCt                    <  1:1927499/150‑1 (MQ=255)
                                                                                                                                     aaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGa                  >  1:488018/1‑150 (MQ=255)
                                                                                                                                         tGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCACAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCTCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTc              <  1:1590968/150‑1 (MQ=255)
                                                                                                                                         tGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGAttta              >  2:468830/1‑149 (MQ=255)
                                                                                                                                         tGAGGCGCTATGCTAGCGCCTATGGTCAGCGGATTAAAGGGCTGCAAAGAGCGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTGCGCTGCGCATTGCGCAATGCCGGGGATCGAAAAAATAAGCGCTGATTTc              <  2:1766993/150‑1 (MQ=255)
                                                                                                                                         ataGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTc              >  1:499344/3‑150 (MQ=255)
                                                                                                                                          taGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt             >  2:418739/2‑150 (MQ=255)
                                                                                                                                          gAGGCTCTATGTTAGCGACTATTGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt             <  2:712800/150‑1 (MQ=255)
                                                                                                                                          gAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt             >  2:1843910/1‑150 (MQ=255)
                                                                                                                                          gAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGCATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt             <  2:1012690/150‑1 (MQ=255)
                                                                                                                                           aGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt            <  2:1812247/150‑1 (MQ=255)
                                                                                                                                           aGGCGCTATGTTAGCGCCTATGGGCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTTCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt            <  1:10412/150‑1 (MQ=255)
                                                                                                                                              cgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGTGCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTTTGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTTAt         <  1:1351598/150‑1 (MQ=255)
                                                                                                                                              cgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAt         >  1:1142926/1‑150 (MQ=255)
                                                                                                                                               gcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtg        <  2:151220/150‑1 (MQ=255)
                                                                                                                                               gcTATGTTAGCGCCTATGGGCAGCGGATTAAAGGGCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtg        <  2:798580/150‑1 (MQ=255)
                                                                                                                                                ctttGTTAGCGCCTATTGTGAGCGGATTAAAGGTCTGCCAAGAGGGATTGCTGCAAACTGCTATCCCTCTCTATGCTTGATGTCTGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGTGAGCGAAAAAATAAGCGCTGATTTCTTAAtgt       <  2:1389619/147‑1 (MQ=255)
                                                                                                                                                cTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCTGGGAGCCAAAAAATTAGCGCTGATTTCTTAAtgt       >  1:388863/1‑150 (MQ=255)
                                                                                                                                                   tGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGAt    <  1:2079753/150‑1 (MQ=255)
                                                                                                                                                    gTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATc   >  2:2175435/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCG  >  NC_000913/2230268‑2230566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: