Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,242,213 T→C 5.6% Q252R (CAA→CGA)  yeiB ← DUF418 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,242,2130TC 5.6% 440.4 / 10.9 142Q252R (CAA→CGA) yeiBDUF418 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (74/60);  new base C (5/3);  total (79/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

CGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCC  >  NC_000913/2242064‑2242360
                                                                                                                                                     |                                                                                                                                                   
cGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCAtt                                                                                                                                                     <  1:246224/150‑1 (MQ=255)
 gATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTg                                                                                                                                                    <  2:63143/150‑1 (MQ=255)
  aTCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGc                                                                                                                                                   <  1:2093837/150‑1 (MQ=255)
     gCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAgg                                                                                                                                                >  1:817751/1‑150 (MQ=255)
       aaGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc                                                                                                                                              <  2:1681329/150‑1 (MQ=255)
       aaGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc                                                                                                                                              <  1:1449733/150‑1 (MQ=255)
        aGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATGGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCg                                                                                                                                             >  1:711480/1‑150 (MQ=255)
        aGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCg                                                                                                                                             >  2:1233129/1‑150 (MQ=255)
           aCCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATg                                                                                                                                          <  1:1716679/150‑1 (MQ=255)
            ccAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATgg                                                                                                                                         >  1:1812501/1‑150 (MQ=255)
             cAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGc                                                                                                                                        >  2:307920/1‑150 (MQ=255)
              aGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATATGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCa                                                                                                                                       <  2:1175924/150‑1 (MQ=255)
               gCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCACCGAAACGCCCAGTCCAGCTGCCATCGCCCTGCAATGGCAg                                                                                                                                      <  2:175441/150‑1 (MQ=255)
               gCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGATCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAg                                                                                                                                      <  2:1634376/150‑1 (MQ=255)
                 ttAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGa                                                                                                                                    >  1:2047252/1‑150 (MQ=255)
                 ttAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATCGCCCTGCAATGGCAgct                                                                                                                                    <  2:1233627/150‑3 (MQ=255)
                  tAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATCGCCCTGCAATGGCAgctg                                                                                                                                   <  1:402592/150‑4 (MQ=255)
                    aaGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCGCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGATgg                                                                                                                                 >  2:2128915/1‑150 (MQ=255)
                    aaGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGTGCGATGGCAGGAAgg                                                                                                                                 <  2:1496640/150‑1 (MQ=255)
                     aGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGt                                                                                                                                <  1:1099961/150‑1 (MQ=255)
                     aGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGt                                                                                                                                >  1:1931420/1‑150 (MQ=255)
                      gCGGCTCAATTGCGGCCAGAGGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt                                                                                                                               <  2:1875191/150‑1 (MQ=255)
                      gCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt                                                                                                                               <  1:1934475/150‑1 (MQ=255)
                      gCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt                                                                                                                               <  1:1535551/150‑1 (MQ=255)
                        ggCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCCCCCCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTaa                                                                                                                             >  1:839125/1‑150 (MQ=255)
                          cTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATCGCAGGGCGATGGCAGGAAGGTTAATg                                                                                                                           >  2:1738560/1‑150 (MQ=255)
                          cTCAATTGCGGCCAGAAGCCATAAAACAGCGAAGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATg                                                                                                                           <  2:1209790/150‑1 (MQ=255)
                           tCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATATGAAGTAAGCAGGCCCACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATgg                                                                                                                          >  1:1782613/1‑150 (MQ=255)
                           acAATTGCGGCCAGAAGCCATAAAACAGCGAAGCATAGCCAATCGCCTGAAAAGGCGCACTCAGGTCCCGCGGCATTTGAAGTAAGAAGGCCCACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATgg                                                                                                                          >  1:468516/2‑150 (MQ=255)
                            cAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGt                                                                                                                         >  1:2004740/1‑150 (MQ=255)
                                tGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCAcc                                                                                                                     <  2:727840/150‑1 (MQ=255)
                                tGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCAcc                                                                                                                     <  2:2093729/150‑1 (MQ=255)
                                tGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCAcc                                                                                                                     <  2:1947755/150‑1 (MQ=255)
                                    gCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCaa                                                                                                                 >  2:1691493/1‑150 (MQ=255)
                                     ccAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCAcccccaa                                                                                                                >  1:186849/1‑147 (MQ=255)
                                       aGAAGCCATAAAACAGCGACGCCTAGCCCATCTCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCg                                                                                                              <  2:1168586/150‑1 (MQ=255)
                                          aGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGcca                                                                                                           <  2:1300105/150‑1 (MQ=255)
                                           gCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGccac                                                                                                          <  2:516769/150‑1 (MQ=255)
                                             cATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGccaccc                                                                                                        >  1:681503/1‑149 (MQ=255)
                                              aTAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAg                                                                                                       <  2:36562/150‑1 (MQ=255)
                                                aaaaCAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGca                                                                                                     >  1:951455/1‑150 (MQ=255)
                                                   aCAGCGACGCATAGCCAATCGCCGGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACaa                                                                                                  <  2:1360238/150‑1 (MQ=255)
                                                    cAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGGTAATGGTCACCCCAATCGCCACCAGCACaaa                                                                                                 <  1:1653750/150‑1 (MQ=255)
                                                      gCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAAc                                                                                               >  1:2020943/1‑150 (MQ=255)
                                                        gACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCa                                                                                             <  1:1233129/150‑1 (MQ=255)
                                                            cATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTac                                                                                         >  1:1976244/1‑150 (MQ=255)
                                                               aGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCCCCAGCACAAAACCAGTacgac                                                                                      >  2:467775/1‑150 (MQ=255)
                                                                gCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAACCCAGTacgacg                                                                                     >  1:818496/1‑150 (MQ=255)
                                                                  cAATCGCCTGAAACGGCGCACTCAGTGCCCGCCGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCACGGCGAAGGCAGGAAGGTTAATGCTCACCCCACACTCCACAAGCACAAACCCACTAcgacgaa                                                                                   >  1:362743/1‑149 (MQ=255)
                                                                      cGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCAATCGCCCCCAGCACAAAACCAGTACGACGATAGTGa                                                                              >  2:854155/1‑150 (MQ=255)
                                                                       gCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGCCGATAGTGa                                                                              >  1:863982/1‑150 (MQ=255)
                                                                         cTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCACTCGCCACCAGCACAAACCCAGAACGACGATAGTGaag                                                                            >  1:1072439/1‑148 (MQ=255)
                                                                            aaaCGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTaa                                                                         >  2:838901/1‑150 (MQ=255)
                                                                              aCGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAgt                                                                       <  1:594166/150‑2 (MQ=255)
                                                                               cGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCt                                                                      <  2:2201831/150‑1 (MQ=255)
                                                                                ggCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTg                                                                     >  2:620879/1‑150 (MQ=255)
                                                                                  cgcACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAACCCAGTACGACGATAGTGACGTAAGCTGaa                                                                   >  1:1570022/1‑150 (MQ=255)
                                                                                   gcACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAAc                                                                  <  2:475936/150‑1 (MQ=255)
                                                                                   gcACTCAGTTCCCGCGGCAGTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAAc                                                                  <  1:333990/150‑1 (MQ=255)
                                                                                    cACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACCCCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGGTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACt                                                                 >  2:821708/1‑150 (MQ=255)
                                                                                       tCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGcc                                                              <  1:771747/150‑1 (MQ=255)
                                                                                          gTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCtt                                                           <  2:1540421/150‑1 (MQ=255)
                                                                                             cccGCGGCATTTGATGTAAGAAGGCACACCAGCGATACGCCCAGTCAAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCCCCCCAAACGCCCCCAGCACAAAACCAGTACGACGTAAGTGACGTACGCTGAACTGccatatcc                                                        >  2:187514/1‑144 (MQ=255)
                                                                                             cccGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTca                                                        >  2:754597/1‑150 (MQ=255)
                                                                                               cgcgGCATTTGAAGTTAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCAGCTGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagc                                                      <  1:1349379/150‑1 (MQ=255)
                                                                                               cgcgGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAACACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagc                                                      >  1:1041588/1‑150 (MQ=255)
                                                                                                 cgGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagcca                                                    >  1:688086/1‑150 (MQ=255)
                                                                                                 cgGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagcca                                                    >  1:1275466/1‑150 (MQ=255)
                                                                                                   gCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCCCCCCAATCGCCACCAGCACAACACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagccagc                                                  >  1:248163/1‑150 (MQ=255)
                                                                                                   gCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagcccgc                                                  >  1:1164105/1‑150 (MQ=255)
                                                                                                   gCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagccagc                                                  <  1:1477377/150‑1 (MQ=255)
                                                                                                   gCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGCTTAATGGTCCCCCCAATCGCCACCCGCACCAAACCAGTACGACGATAGTGACGTAAGCTGACCTGCCCTTTcagccagc                                                  >  1:127306/1‑150 (MQ=255)
                                                                                                    cATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCagccagcc                                                 <  1:632125/150‑1 (MQ=255)
                                                                                                      tttGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGc                                               <  1:924169/150‑1 (MQ=255)
                                                                                                         gAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgc                                            >  2:627618/1‑150 (MQ=255)
                                                                                                         gAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgc                                            >  2:228140/1‑150 (MQ=255)
                                                                                                           aGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgcgc                                          >  1:1718843/1‑150 (MQ=255)
                                                                                                            gTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCCCCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCa                                         >  1:1323011/1‑150 (MQ=255)
                                                                                                               agaagGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCa                                      >  1:486320/1‑150 (MQ=255)
                                                                                                                    gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTCCGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGcgcc                                 >  1:1154164/1‑150 (MQ=255)
                                                                                                                    gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGcgcc                                 <  1:1539261/150‑1 (MQ=255)
                                                                                                                       cacCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCa                              <  2:817751/150‑1 (MQ=255)
                                                                                                                         ccAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATTGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCAcc                            <  1:1668024/150‑1 (MQ=255)
                                                                                                                            gCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAAt                         >  1:1058985/1‑150 (MQ=255)
                                                                                                                             cGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATg                        <  1:2170924/150‑1 (MQ=255)
                                                                                                                              gATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGa                       >  1:646629/1‑150 (MQ=255)
                                                                                                                                tACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGc                     <  2:2020943/150‑1 (MQ=255)
                                                                                                                                 aCGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGca                    <  2:2050639/150‑1 (MQ=255)
                                                                                                                                  cGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCACTGAGcaa                   >  2:1421946/1‑149 (MQ=255)
                                                                                                                                       aGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAAAGAGCATCATcc              <  2:1487268/150‑1 (MQ=255)
                                                                                                                                          cttccTGCCATCGCCCTGCAATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATGCTCCCTg           >  1:2182099/5‑150 (MQ=255)
                                                                                                                                          cttccTGCCATCGCCCTGCAATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTg           >  1:761100/5‑150 (MQ=255)
                                                                                                                                          cttccTGCCATCGCCCTGCAATGGCAGGAAGGTTAATGGTCACCCAAATCGCCACCAGCACAAAACAAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTg           >  2:1512293/5‑150 (MQ=255)
                                                                                                                                          ccAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTg           <  2:1608957/150‑1 (MQ=255)
                                                                                                                                           cAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCACTCGCCACCAGCACAACACCAGTACGACGATAGTGACGTAAGCTGCACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGCGCATCAGCCCTGc          >  1:375559/1‑150 (MQ=255)
                                                                                                                                               tGCCATTGCAGGGCGATGGCAGGAAGGTTACTGGTCACGCCAATCTCCAACAGCACAAAACAAGTCCGACGATAGTGAAGAAAGCTGAACTGCCCGTTCCGCCAGCCGCTGCGCATCAGCGCCGCCCCAATGAGCATCCTCCCTGCCAGt      >  2:321280/1‑150 (MQ=255)
                                                                                                                                                gCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGtt     >  2:1619152/1‑150 (MQ=255)
                                                                                                                                                  cATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCATTCGCCACCAGCACAAAACCAGTACGACGATAGTTACGTAAGCAGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGGATCATCCCTGCCATTTgc   <  2:468516/150‑1 (MQ=255)
                                                                                                                                                  cATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgc   >  1:1108761/1‑150 (MQ=255)
                                                                                                                                                   aTTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgcc  <  1:492080/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCC  >  NC_000913/2242064‑2242360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: