Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,242,218 G→T 5.6% A250A (GCC→GCA yeiB ← DUF418 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,242,2180GT 5.6% 438.7 / 10.4 142A250A (GCC→GCAyeiBDUF418 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (74/59);  new base T (4/4);  total (79/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-01

Only 100 of 148 total aligned reads displayed.

GCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCCAGCCATAC  >  NC_000913/2242069‑2242368
                                                                                                                                                     |                                                                                                                                                      
gCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAgg                                                                                                                                                        >  1:817751/1‑150 (MQ=255)
   aGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATGGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCg                                                                                                                                                     >  1:711480/1‑150 (MQ=255)
   aGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCg                                                                                                                                                     >  2:1566399/1‑150 (MQ=255)
   aGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCg                                                                                                                                                     >  2:1233129/1‑150 (MQ=255)
      aCCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATg                                                                                                                                                  <  1:1716679/150‑1 (MQ=255)
       ccAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATgg                                                                                                                                                 >  1:1812501/1‑150 (MQ=255)
        cAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGc                                                                                                                                                >  2:307920/1‑150 (MQ=255)
         aGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATATGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCa                                                                                                                                               <  2:1175924/150‑1 (MQ=255)
          gCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCACCGAAACGCCCAGTCCAGCTGCCATCGCCCTGCAATGGCAg                                                                                                                                              <  2:175441/150‑1 (MQ=255)
          gCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGATCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAg                                                                                                                                              <  2:1634376/150‑1 (MQ=255)
            ttAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGa                                                                                                                                            >  1:2047252/1‑150 (MQ=255)
            ttAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATCGCCCTGCAATGGCAgct                                                                                                                                            <  2:1233627/150‑3 (MQ=255)
             tAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATCGCCCTGCAATGGCAgctg                                                                                                                                           <  1:402592/150‑4 (MQ=255)
                aGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAACCGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCCCACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGt                                                                                                                                        >  2:1624256/1‑150 (MQ=255)
                aGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGt                                                                                                                                        >  1:1931420/1‑150 (MQ=255)
                aGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGt                                                                                                                                        <  1:1099961/150‑1 (MQ=255)
                 gCGGCTCAATTGCGGCCAGAGGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt                                                                                                                                       <  2:1875191/150‑1 (MQ=255)
                     cTCAATTGCGGCCAGAAGCCATAAAACAGCGAAGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATg                                                                                                                                   <  2:1209790/150‑1 (MQ=255)
                      tCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATATGAAGTAAGCAGGCCCACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATgg                                                                                                                                  >  1:1782613/1‑150 (MQ=255)
                      acAATTGCGGCCAGAAGCCATAAAACAGCGAAGCATAGCCAATCGCCTGAAAAGGCGCACTCAGGTCCCGCGGCATTTGAAGTAAGAAGGCCCACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATgg                                                                                                                                  >  1:468516/2‑150 (MQ=255)
                       cAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGt                                                                                                                                 >  1:2004740/1‑150 (MQ=255)
                           tGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCAcc                                                                                                                             <  2:2093729/150‑1 (MQ=255)
                             cGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCAcccc                                                                                                                           >  2:677850/1‑150 (MQ=255)
                               gCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCaa                                                                                                                         >  2:1691493/1‑150 (MQ=255)
                               gCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCaa                                                                                                                         >  1:1419388/1‑150 (MQ=255)
                                  aGAAGCCATAAAACAGCGTCGCATAGCCTATCGCCTGAAACGGCGCACTCATTTCCCGCGTCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCTATGGCAGGAAGGTTAATGGTCACCCCAATCg                                                                                                                      <  1:1940144/150‑1 (MQ=255)
                                     aGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGcca                                                                                                                   <  2:1300105/150‑1 (MQ=255)
                                      gCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGccac                                                                                                                  <  2:516769/150‑1 (MQ=255)
                                       ccATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGccacc                                                                                                                 >  1:1487268/1‑150 (MQ=255)
                                        cATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGccaccc                                                                                                                >  1:681503/1‑149 (MQ=255)
                                         aTAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAg                                                                                                               <  2:36562/150‑1 (MQ=255)
                                           aaaaCAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGca                                                                                                             >  1:951455/1‑150 (MQ=255)
                                              aCAGCGACGCATAGCCAATCGCCGGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACaa                                                                                                          <  2:1360238/150‑1 (MQ=255)
                                               cAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGGTAATGGTCACCCCAATCGCCACCAGCACaaa                                                                                                         <  1:1653750/150‑1 (MQ=255)
                                                 gCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAAc                                                                                                       >  1:2020943/1‑150 (MQ=255)
                                                  cGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCAATTGAAGAAAGAAGGAACACAAGCTATACGCCCAGACCAGATGCCATTGCAGAGCGAAGGCAGGAAGGTTAATGCTCACCCACATCGCCACCACCACAAAccc                                                                                                      >  1:130524/1‑150 (MQ=255)
                                                   gACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCa                                                                                                     <  1:1233129/150‑1 (MQ=255)
                                                       cATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTac                                                                                                 >  1:1976244/1‑150 (MQ=255)
                                                       cATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTac                                                                                                 >  1:1917696/1‑150 (MQ=255)
                                                           gCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAACCCAGTacgacg                                                                                             >  1:818496/1‑150 (MQ=255)
                                                             cAATCGCCTGAAACGGCGCACTCAGTGCCCGCCGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCACGGCGAAGGCAGGAAGGTTAATGCTCACCCCACACTCCACAAGCACAAACCCACTAcgacgaa                                                                                           >  1:362743/1‑149 (MQ=255)
                                                                 cGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCAATCGCCCCCAGCACAAAACCAGTACGACGATAGTGa                                                                                      >  2:854155/1‑150 (MQ=255)
                                                                  gCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGCCGATAGTGa                                                                                      >  1:863982/1‑150 (MQ=255)
                                                                  gCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGa                                                                                      <  2:1726743/150‑1 (MQ=255)
                                                                   ccTGAAACGTCGCACTCAGTACCCCCGGCATTTGAACGAAGAAGGCACACAAGCGAGACGCCCAGACCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGTTCACCCCAATCGCCACCAGCACAAAACCAGTACCACGATAGTGAc                                                                                     <  2:420436/150‑1 (MQ=255)
                                                                   ccTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCCGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGCAAGGTTAATGGTCACCCCACTCGCCACCCGCACAACACCAGTACGACGATAGTGAc                                                                                     >  1:267297/1‑150 (MQ=255)
                                                                    cTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCACTCGCCACCAGCACAAACCCAGAACGACGATAGTGaag                                                                                    >  1:1072439/1‑148 (MQ=255)
                                                                    cTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACg                                                                                    <  1:826217/150‑1 (MQ=255)
                                                                     tGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGt                                                                                   <  2:2055631/150‑1 (MQ=255)
                                                                         aCGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAgt                                                                               <  1:594166/150‑2 (MQ=255)
                                                                          cGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCt                                                                              <  2:2201831/150‑1 (MQ=255)
                                                                           ggCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTg                                                                             >  1:647858/1‑150 (MQ=255)
                                                                            gcgcACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAACCCAGTACGACGATAGTGCCGTAAGCTGa                                                                            >  2:1925679/1‑150 (MQ=255)
                                                                             cgcACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAACCCAGTACGACGATAGTGACGTAAGCTGaa                                                                           >  1:1570022/1‑150 (MQ=255)
                                                                               cACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACCCCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGGTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACt                                                                         >  2:821708/1‑150 (MQ=255)
                                                                               cACTCAGTTCCCGAGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGCTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACt                                                                         <  2:942310/150‑1 (MQ=255)
                                                                                  tCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGcc                                                                      <  1:771747/150‑1 (MQ=255)
                                                                                   cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGTTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGccc                                                                     <  2:593610/150‑1 (MQ=255)
                                                                                   cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGccc                                                                     <  1:699322/150‑1 (MQ=255)
                                                                                        cccGCGGCATTTGATGTAAGAAGGCACACCAGCGATACGCCCAGTCAAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCCCCCCAAACGCCCCCAGCACAAAACCAGTACGACGTAAGTGACGTACGCTGAACTGccatatcc                                                                >  2:187514/1‑144 (MQ=255)
                                                                                        cccGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcc                                                                >  2:295751/1‑149 (MQ=255)
                                                                                        cccGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTca                                                                >  2:754597/1‑150 (MQ=255)
                                                                                          cgcgGCATTTGAAGTTAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCAGCTGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagc                                                              <  1:1349379/150‑1 (MQ=255)
                                                                                          cgcgGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAACACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagc                                                              >  1:1041588/1‑150 (MQ=255)
                                                                                            cgGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagcca                                                            >  1:1275466/1‑150 (MQ=255)
                                                                                              gCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCCCCCCAATCGCCACCAGCACAACACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagccagc                                                          >  1:248163/1‑150 (MQ=255)
                                                                                              gCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagcccgc                                                          >  1:1164105/1‑150 (MQ=255)
                                                                                              gCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagccagc                                                          <  1:1477377/150‑1 (MQ=255)
                                                                                               cATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCagccagcc                                                         <  1:632125/150‑1 (MQ=255)
                                                                                                    gAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgc                                                    >  2:1099245/1‑150 (MQ=255)
                                                                                                    gAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgc                                                    >  2:627618/1‑150 (MQ=255)
                                                                                                      aGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgcgc                                                  >  2:82307/1‑150 (MQ=255)
                                                                                                      aGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgcgc                                                  >  1:1718843/1‑150 (MQ=255)
                                                                                                         aagaagGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATc                                               <  2:1787217/150‑1 (MQ=255)
                                                                                                          agaagGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCa                                              >  1:486320/1‑150 (MQ=255)
                                                                                                          agaagGCACACCAGCGATACGCCCAGTCCAACTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCa                                              <  1:400307/150‑1 (MQ=255)
                                                                                                               gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTCCGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGcgcc                                         >  1:1154164/1‑150 (MQ=255)
                                                                                                               gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGcgcc                                         <  1:1539261/150‑1 (MQ=255)
                                                                                                                    ccAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATTGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCAcc                                    <  1:1668024/150‑1 (MQ=255)
                                                                                                                       gCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAAt                                 >  1:1058985/1‑150 (MQ=255)
                                                                                                                        cGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCTCCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATg                                <  2:2169162/150‑1 (MQ=255)
                                                                                                                        cGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATg                                <  1:2170924/150‑1 (MQ=255)
                                                                                                                           tACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGc                             <  1:920625/150‑1 (MQ=255)
                                                                                                                           tACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGc                             <  2:2020943/150‑1 (MQ=255)
                                                                                                                             cGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCCCCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGcat                           >  1:767569/1‑150 (MQ=255)
                                                                                                                             cGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCACTGAGcaa                           >  2:1421946/1‑149 (MQ=255)
                                                                                                                                  aGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAAAGAGCATCATcc                      <  2:1487268/150‑1 (MQ=255)
                                                                                                                                     cttccTGCCATCGCCCTGCAATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATGCTCCCTg                   >  1:2182099/5‑150 (MQ=255)
                                                                                                                                     cttccTGCCATCGCCCTGCAATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACAAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCCATGAGCATCATCCCTg                   >  2:150893/5‑150 (MQ=255)
                                                                                                                                     cttccTGCCATCGCCCTGCAATGGCAGGAAGGTTAATGGTCACCCAAATCGCCACCAGCACAAAACAAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTg                   >  2:1512293/5‑150 (MQ=255)
                                                                                                                                     ccAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTg                   <  2:1608957/150‑1 (MQ=255)
                                                                                                                                      cAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCACTCGCCACCAGCACAACACCAGTACGACGATAGTGACGTAAGCTGCACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGCGCATCAGCCCTGc                  >  1:375559/1‑150 (MQ=255)
                                                                                                                                         cTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAg               >  2:793186/1‑150 (MQ=255)
                                                                                                                                             cATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCATTCGCCACCAGCACAAAACCAGTACGACGATAGTTACGTAAGCAGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGGATCATCCCTGCCATTTgc           <  2:468516/150‑1 (MQ=255)
                                                                                                                                             cATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgc           <  2:468518/150‑1 (MQ=255)
                                                                                                                                             cATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgc           >  1:1108761/1‑150 (MQ=255)
                                                                                                                                              aTTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgcc          <  1:492080/150‑1 (MQ=255)
                                                                                                                                                  cAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgccagcc      <  1:1264367/150‑1 (MQ=255)
                                                                                                                                                   aGGGCGATGGCAGGAAGGTTAATGGGCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgccagcca     <  1:447782/150‑1 (MQ=255)
                                                                                                                                                    gggCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCCAGCCAt    <  2:2004740/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCCAGCCATAC  >  NC_000913/2242069‑2242368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: