Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,312,170 G→C 6.8% L194V (CTG→GTG)  ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,312,1700GC 6.8% 447.4 / 11.5 135L194V (CTG→GTG) ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (78/47);  new base C (7/3);  total (85/50)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACG  >  NC_000913/2312024‑2312317
                                                                                                                                                  |                                                                                                                                                   
ttAGGTTTCAGCACGGTCGCCGTTACAGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGc                                                                                                                                                  <  2:1546041/149‑1 (MQ=255)
gtAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGTAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGc                                                                                                                                                  <  2:1553928/150‑1 (MQ=255)
gtAGGTTTCAGCACGGTCGCCGTTACCGATGAAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGAAAGCACCGCCGACGCCGTCGCCGTTTAGCCCCAGTGc                                                                                                                                                  >  1:1400558/1‑150 (MQ=255)
   ggTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCTTCAGTACGTTTGGAGCTGGAGATCGCAACACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTc                                                                                                                                               <  2:620922/150‑1 (MQ=255)
   ggTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTc                                                                                                                                               >  2:1464210/1‑150 (MQ=255)
        cAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGAATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCACTGCGTCaaaac                                                                                                                                          <  2:1217046/150‑5 (MQ=255)
         aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCCCCACCGATACCGAACCCTTCGGAATCATAAGTGATATAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGAcc                                                                                                                                         >  1:1366410/1‑150 (MQ=255)
         aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGAcc                                                                                                                                         >  2:523118/1‑150 (MQ=255)
          gCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCg                                                                                                                                        >  1:1921647/1‑150 (MQ=255)
          gCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCg                                                                                                                                        >  1:1823026/1‑150 (MQ=255)
          gCACGGGCGCCGTTACCGATTTAAGCAGCGGTGTTCTGAGCATCAGTACGGTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCACTGCGTCaaaacgg                                                                                                                                        <  2:1333173/150‑7 (MQ=255)
            aCGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGCCCgtt                                                                                                                                      >  2:538256/1‑150 (MQ=255)
             cGGTCGCCGTTACCTATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCgttg                                                                                                                                     <  1:2165593/150‑1 (MQ=255)
             cGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCgttg                                                                                                                                     <  1:885262/150‑1 (MQ=255)
               gTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCgttgtt                                                                                                                                   >  1:1087483/1‑150 (MQ=255)
               gTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCAGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCACTGCGTCACGACCgatgtt                                                                                                                                   >  1:396224/1‑150 (MQ=255)
                 cGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTgttag                                                                                                                                 <  2:1427675/150‑1 (MQ=255)
                   ccGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATAACGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTgttagtt                                                                                                                               >  2:2174374/1‑150 (MQ=255)
                             tGTAAGCAGCTGTGTTCTGAGCAGCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAg                                                                                                                     <  1:1294364/150‑1 (MQ=255)
                              gTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCAACACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCCCCGACGACGTCGCCGTTTTGACGCAGTGCGTCACGACCTTTGTTAGTTAAGCCACAAGt                                                                                                                    >  1:726101/1‑150 (MQ=255)
                               tAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTa                                                                                                                   >  1:661505/1‑150 (MQ=255)
                                   cagcGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGCCCGTTGTTAGTTACGCCACTAGTAAAGc                                                                                                               >  1:435144/1‑150 (MQ=255)
                                    agcGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGcc                                                                                                              <  1:1908763/150‑1 (MQ=255)
                                          gtTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCAcc                                                                                                        <  1:492983/150‑1 (MQ=255)
                                          gtTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCAcc                                                                                                        >  2:1814682/1‑150 (MQ=255)
                                             cTGAGCATCAGTACGTTTGGCGCTGGAGATCGCACCCCCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTGACGACAGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGa                                                                                                     >  2:1073935/1‑150 (MQ=255)
                                             cTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGa                                                                                                     >  2:184588/1‑150 (MQ=255)
                                              tGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGAt                                                                                                    >  1:1744035/1‑150 (MQ=255)
                                               gAGCATCAGTACGTTTGGAGCTGGATTTCTCACCACAGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCTACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATg                                                                                                   <  1:1329890/150‑1 (MQ=255)
                                               caGCATCAGTACGTTTCGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGAGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTAGTTAGTTACGCCCCTAGTAAAGCCTTCACCAGATg                                                                                                   >  1:213554/2‑150 (MQ=255)
                                                    tCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTg                                                                                              >  2:613560/1‑150 (MQ=255)
                                                      aGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGACACCTTCGTAATCATTAGTGATAGCACCGCCGACGCCGTCACCGTTTTGAAGCACTGCGTCACGACCGGTGTGAGTTACGCCCCTAGTAAAGCCTTCACCAGATGGGTTGcc                                                                                            >  1:119304/1‑150 (MQ=255)
                                                       gTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TCGTAATAATAAGTGATAGAACCGCCGACGCCGTCGCCGTATTGACCCACTGCGTCACGACCCTTGTTAGTTACGCAACTAGTAAAGCCATCACCAGATGGGTTGcctt                                                                                          >  1:1799926/1‑148 (MQ=255)
                                                         aCGTTTGGAGCTGGAGATCGCCCCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAATGCCTTCACCAGATGGGTTGCCGtt                                                                                         <  2:908902/150‑1 (MQ=255)
                                                         aCGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGtt                                                                                         >  1:752031/1‑150 (MQ=255)
                                                          cGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGttt                                                                                        >  1:2138656/1‑150 (MQ=255)
                                                           gTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGtttt                                                                                       >  2:2070760/1‑150 (MQ=255)
                                                            tttGGAGCTGGAGATCGCACCACCTATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGttttt                                                                                      <  2:557794/150‑1 (MQ=255)
                                                            tttGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGAGTAACGACCGTTGGAAGTTACGCCACTAGTATAGCCTTCACCATATGGGTGGCCGttttt                                                                                      >  2:357838/1‑150 (MQ=255)
                                                               ggAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAcc                                                                                   <  2:1921647/150‑1 (MQ=255)
                                                                gAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                                                                                  <  1:607341/150‑1 (MQ=255)
                                                                  gCTGGAGATCTCACCACCGATAACGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTGGCCGTTTTGTCGCAGTGGGTCACGTCCGTTGTTAGTTACGCCTCTAGTAAAGCCTTCACCAGATGGTTTGCCGTTTTTACCCTg                                                                                <  1:1591858/150‑1 (MQ=255)
                                                                    tGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGt                                                                              <  1:1506171/150‑1 (MQ=255)
                                                                    tGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGt                                                                              >  2:1780286/1‑150 (MQ=255)
                                                                     ggAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTa                                                                             >  1:1194011/1‑150 (MQ=255)
                                                                     ggAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGACTTCACCAGATGGGTTGCCGTTTTTACCCTGGTa                                                                             >  2:1652268/1‑150 (MQ=255)
                                                                      gagaTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTAc                                                                            <  2:625968/150‑1 (MQ=255)
                                                                        gaTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTg                                                                          >  1:804543/1‑150 (MQ=255)
                                                                        gaTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTg                                                                          >  1:1475792/1‑150 (MQ=255)
                                                                        gaTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTg                                                                          >  1:215513/1‑150 (MQ=255)
                                                                         aTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGa                                                                         <  2:2092979/150‑1 (MQ=255)
                                                                          tCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa                                                                        >  2:2185375/1‑150 (MQ=255)
                                                                           cGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAAc                                                                       <  2:1632657/150‑1 (MQ=255)
                                                                            gCACCACGGATACCGAACCCATCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACa                                                                      <  2:438537/150‑1 (MQ=255)
                                                                                 accGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCaaa                                                                 >  1:1913530/1‑150 (MQ=255)
                                                                                  ccGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCGGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg                                                                <  2:1998897/150‑1 (MQ=255)
                                                                                  ccGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg                                                                <  2:1802640/150‑1 (MQ=255)
                                                                                   cGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGt                                                               >  1:1358364/1‑150 (MQ=255)
                                                                                    gATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt                                                              >  2:2038405/1‑150 (MQ=255)
                                                                                     aTACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTc                                                             <  1:523118/150‑1 (MQ=255)
                                                                                       aCCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg                                                           <  2:2160359/150‑1 (MQ=255)
                                                                                        ccGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg                                                          >  1:969988/1‑150 (MQ=255)
                                                                                         cGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCCCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc                                                         >  2:393129/1‑150 (MQ=255)
                                                                                         cGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc                                                         >  1:1504694/1‑150 (MQ=255)
                                                                                         cGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATAGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc                                                         <  1:821506/150‑1 (MQ=255)
                                                                                             aCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTc                                                     <  2:1164599/150‑1 (MQ=255)
                                                                                               cTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                   >  1:1232475/1‑150 (MQ=255)
                                                                                               cTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                   <  2:2088063/150‑1 (MQ=255)
                                                                                               attCGTAATCTTAAGTTATAGAACCGCCTACGCCGGCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                   <  1:1388259/149‑1 (MQ=255)
                                                                                                  cGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                >  2:931974/1‑150 (MQ=255)
                                                                                                    tAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGACACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGATCAGCAAAGTTCAGGCCGTCAACCAGa                                              >  2:185411/1‑150 (MQ=255)
                                                                                                      aTCATAAGGGAAAGAACCGCCGACGCCGGCGCCGTTTTGACGCTGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGGCAACCAGAcc                                            <  2:1110106/150‑1 (MQ=255)
                                                                                                       tCATAAGTGATAGAACCGCCGACGCCGTCGAAGTTTTGACGCAGTGCGTCACGACAGTTGTTATTTACGCCACTAGTAAATCCTTCACCAGATGGGTTGCCGTTTTTACACTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGAACg                                           >  1:2204126/1‑150 (MQ=255)
                                                                                                        cATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCga                                          >  2:2090420/1‑150 (MQ=255)
                                                                                                             gTGATCGAAACGCCGACGCCGGCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                     <  2:1087483/150‑1 (MQ=255)
                                                                                                             gTGATAGAACCGCCGACGCCGTGGCCGTTTTGACGCAGTGTTTAACTTGTTTTTTTATTTTCGCCACTAGTAAAGTATTCAACTTATGTGTTTAAGTTTTTACGATGGTATTGATATGTTTATTTCAGGCAGGGAAGCAGACCGaagaag                                     <  2:287618/150‑1 (MQ=255)
                                                                                                             gTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                     >  1:1049096/1‑150 (MQ=255)
                                                                                                             gTGATAGAACCACCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCTCTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                     <  2:458369/150‑1 (MQ=255)
                                                                                                               gATAGAACCGCCGCCGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCCGGCCGTCAACCTGACCGaagaaggc                                   >  1:1937927/1‑148 (MQ=255)
                                                                                                               gATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc                                   >  1:2163586/1‑150 (MQ=255)
                                                                                                                   gAACCGACGACGCCGTCGCCGTTTTGCCGCAGTGCGTCACGAACGTTGTTAGTTACGCCAATAGTTCAGCCATCAGCAGATGGGGTGACGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCATCCAGACCGAAGCAGTCAgtg                               >  1:332328/1‑150 (MQ=255)
                                                                                                                           gACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTa                       <  2:1453116/150‑1 (MQ=255)
                                                                                                                               ccgtcgccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTc                   >  1:961092/1‑150 (MQ=255)
                                                                                                                                   cgccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGa               >  1:1138778/1‑150 (MQ=255)
                                                                                                                                    gccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa              >  2:1525844/1‑150 (MQ=255)
                                                                                                                                    gccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa              >  1:1476067/1‑150 (MQ=255)
                                                                                                                                     cggtcgtGACGCCCTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGGCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg             >  1:997393/7‑150 (MQ=255)
                                                                                                                                     cggtcgtGACGCACTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg             >  2:493188/7‑150 (MQ=255)
                                                                                                                                     ccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg             >  2:149909/1‑150 (MQ=255)
                                                                                                                                     ccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGGCGCGAAg             <  1:613560/150‑1 (MQ=255)
                                                                                                                                      ggtcgtGACGCACTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc            >  2:1720708/6‑150 (MQ=255)
                                                                                                                                      gggtttGACGCAGTGCGTCACGACCGTTGGTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc            <  1:605821/147‑1 (MQ=255)
                                                                                                                                       gtcgtGACGCACTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAACTCAGTGTTACGGTAGGTCGCGAAGcc           >  1:204419/5‑150 (MQ=255)
                                                                                                                                        ttttGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCTTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg          <  2:1135068/150‑1 (MQ=255)
                                                                                                                                             aCGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAcc     <  1:1464210/150‑1 (MQ=255)
                                                                                                                                              cGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAccc    >  1:1622000/1‑149 (MQ=255)
                                                                                                                                              cGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa    <  2:1912012/150‑1 (MQ=255)
                                                                                                                                               gCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc   >  1:1963934/1‑150 (MQ=255)
                                                                                                                                               gCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc   >  1:1661157/1‑150 (MQ=255)
                                                                                                                                                cAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACg  >  2:1870344/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACG  >  NC_000913/2312024‑2312317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑