Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,319,581 A→G 6.5% V99A (GTG→GCG) ‡ rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,319,5810AG 6.5% 406.2 / 13.8 139V99A (GTG→GCG) ‡rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base A (62/67);  new base G (6/3);  total (69/70)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01

Only 100 of 144 total aligned reads displayed.

CGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTG  >  NC_000913/2319432‑2319729
                                                                                                                                                     |                                                                                                                                                    
cGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCCCGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGca                                                                                                                                                      <  1:2006753/150‑1 (MQ=255)
  aTTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGACAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGcaca                                                                                                                                                    <  1:1065742/150‑1 (MQ=255)
      aaaaTACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAACCCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATccc                                                                                                                                                >  2:2197663/1‑149 (MQ=255)
      aaaaTACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCg                                                                                                                                                >  2:1611883/1‑150 (MQ=255)
       aaaTACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGTTTCAAACGCAGGCACATCCGc                                                                                                                                               >  1:1129125/1‑150 (MQ=255)
            cccGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCg                                                                                                                                          >  1:1040932/1‑150 (MQ=255)
             ccGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGt                                                                                                                                         >  2:567728/1‑150 (MQ=255)
              cGGTTTAGATCGTAAGCCGCAGTAAAATTATCGCGCCAGTAGCGCATAAACCACGCCTATGACTCCTGAGAACCTCGCCAGTTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGtt                                                                                                                                        <  2:1345324/150‑1 (MQ=255)
              cGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCGGATGTGCCTGCGtt                                                                                                                                        <  2:475173/150‑1 (MQ=255)
              cGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCGGATGTGCCTGCGtt                                                                                                                                        <  2:1509036/150‑1 (MQ=255)
                gTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtc                                                                                                                                      <  1:460079/150‑1 (MQ=255)
                 tttAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATTACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtct                                                                                                                                     <  1:1637385/150‑1 (MQ=255)
                    aGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGt                                                                                                                                  >  1:2112797/1‑150 (MQ=255)
                     gATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTc                                                                                                                                 <  2:1530669/150‑1 (MQ=255)
                     gATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTAGTc                                                                                                                                 >  1:118928/1‑150 (MQ=255)
                      aTCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTcc                                                                                                                                >  1:526921/1‑150 (MQ=255)
                       tCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCa                                                                                                                               <  1:661059/150‑1 (MQ=255)
                       tCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCa                                                                                                                               >  1:1952648/1‑150 (MQ=255)
                             gCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCggg                                                                                                                         <  2:1652072/150‑1 (MQ=255)
                             gCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCggg                                                                                                                         >  1:11404/1‑150 (MQ=255)
                                 cAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGaaa                                                                                                                     >  2:1184829/1‑150 (MQ=255)
                                          tATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCATGCGTTTCTCGTCCACGCGGGGAAAGCACACCGt                                                                                                            >  2:1280142/1‑150 (MQ=255)
                                            tCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGttt                                                                                                          <  2:2195721/150‑1 (MQ=255)
                                            tCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGGCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGttt                                                                                                          <  1:1828553/150‑1 (MQ=255)
                                             cgcgCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCCCGCGGGGAAAGCACACCGtttt                                                                                                         >  1:1304604/1‑150 (MQ=255)
                                               cgcCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCCCGCGGGGAAAGCACACCGTTTTCt                                                                                                       >  2:1986629/1‑150 (MQ=255)
                                               cgcCAGTAGCGCATAAACCACGACAATGCCTCCAGAGAACCTCGCCAGTTGTTACTCATTGCGGAACAAGCGGAGTCGGCCAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCt                                                                                                       <  2:1495999/150‑1 (MQ=255)
                                                gcCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTg                                                                                                      <  2:2112797/150‑1 (MQ=255)
                                                     tAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGAt                                                                                                 <  1:1343508/150‑1 (MQ=255)
                                                     tAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGAt                                                                                                 >  2:2119/1‑150 (MQ=255)
                                                         gcATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCTTTGCGGAACACTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACCTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACCCCGTTTTCTGCCGATAAGc                                                                                             >  2:766433/1‑150 (MQ=255)
                                                         gcATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGc                                                                                             <  2:2047823/150‑1 (MQ=255)
                                                         gcATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGc                                                                                             <  1:1757275/150‑1 (MQ=255)
                                                         gcATAAACCACGCCAATGACTACAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGc                                                                                             <  1:1380515/150‑1 (MQ=255)
                                                         gcATAAACACCGCCACTGACTCCAGCGAACCTCGCCAGGTGTAACGCATAGCGGAACAATCGGAGTCGGAAAACAGCGGGTCAAACTCAGGCACATCCGCCGGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGc                                                                                             <  2:565421/150‑1 (MQ=255)
                                                          cATAAACCACGCCAATGCCTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGGGTTTCTCGTCAACGCGGGGAACGCACACCGTTTTCTGCCGATAAGCg                                                                                            >  2:580977/1‑150 (MQ=255)
                                                          cATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCg                                                                                            >  2:1439446/1‑150 (MQ=255)
                                                              aaCCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGAttt                                                                                        <  1:184943/150‑1 (MQ=255)
                                                                   cGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATACGCGATTTTCGGc                                                                                   >  1:604960/1‑150 (MQ=255)
                                                                   cGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATATTCGGc                                                                                   >  1:423181/1‑150 (MQ=255)
                                                                    gCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCg                                                                                  <  1:296601/150‑1 (MQ=255)
                                                                     ccAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGGCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGa                                                                                 <  2:498237/150‑1 (MQ=255)
                                                                       aaTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACTCTGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATg                                                                               <  1:171546/150‑1 (MQ=255)
                                                                       aaTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATg                                                                               <  2:774943/150‑1 (MQ=255)
                                                                       aaTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATg                                                                               >  2:1951342/1‑150 (MQ=255)
                                                                        aTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCTATTTTCGGCGATGt                                                                              >  2:188052/1‑150 (MQ=255)
                                                                          gACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTAc                                                                            <  2:1906903/150‑1 (MQ=255)
                                                                            cTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACtt                                                                          >  1:966079/1‑150 (MQ=255)
                                                                              ccAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAACGCACACGGTGTTCTGCCGATACGCGATTTTCGGCGATGTGCTTCa                                                                        >  1:336004/1‑150 (MQ=255)
                                                                              ccAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCa                                                                        >  1:1137603/1‑150 (MQ=255)
                                                                              ccAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCa                                                                        <  1:828981/150‑1 (MQ=255)
                                                                              ccAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGAACTTCa                                                                        >  1:2029463/1‑150 (MQ=255)
                                                                               cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                       <  2:684457/150‑1 (MQ=255)
                                                                               cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                       >  1:732683/1‑150 (MQ=255)
                                                                               cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                       >  2:1211990/1‑150 (MQ=255)
                                                                               cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACAACGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                       >  2:1414867/1‑150 (MQ=255)
                                                                                agagAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGc                                                                      <  2:967043/150‑1 (MQ=255)
                                                                                     aCCTCGCCAGGTGTTACTCAATGCGGAACAATAGGAGTCGGCAAACAGCGGTTCAAACTCCGGCACATCCGCATGCGTTTCTCGTCCACGCGGGGTAAGCACACCGTTTTCTGCCGATAAGCGATTTTAGGCGATGTACTAAAGCTCttt                                                                 >  2:1691659/1‑150 (MQ=255)
                                                                                      ccTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTtc                                                                <  2:231068/150‑1 (MQ=255)
                                                                                      ccTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTtc                                                                >  2:1886032/1‑150 (MQ=255)
                                                                                       cTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTtca                                                               <  1:1225638/150‑1 (MQ=255)
                                                                                            cAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTcatcaca                                                          <  1:21147/150‑1 (MQ=255)
                                                                                              ggTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTcatcacatc                                                        <  2:182497/150‑1 (MQ=255)
                                                                                                   tACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCCCGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAgg                                                   >  2:917196/1‑150 (MQ=255)
                                                                                                    aCTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGa                                                                                 <  1:605178/119‑1 (MQ=255)
                                                                                                     cTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAGGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCTATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTg                                                 <  1:2195455/150‑1 (MQ=255)
                                                                                                       cATTGCGGAACAATCGGAGTCGGCAACCAGCGGTTCAAACGCAGGCACATCCGCCTGCGATTCTCGTCCACGCGGGGCAAGCACACCGTTTTCTCGCGATAAGCGATTTTCGGCGATGTACTTCGGCTCTTGCATCACATCAGAGGTGCg                                               >  2:372032/1‑150 (MQ=255)
                                                                                                        aTTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGtt                                             <  1:792694/150‑1 (MQ=255)
                                                                                                         ttGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGtt                                             >  2:52016/1‑150 (MQ=255)
                                                                                                          tGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTg                                            <  1:2020572/150‑1 (MQ=255)
                                                                                                           gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGa                                           >  1:483624/1‑150 (MQ=255)
                                                                                                            cGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTAAGCTCTTTCATCACATCATAGGTGCGTTGaa                                          >  2:437896/1‑150 (MQ=255)
                                                                                                             ggAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAAt                                         <  1:1611883/150‑1 (MQ=255)
                                                                                                                aCAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATaaa                                      <  2:1700036/150‑1 (MQ=255)
                                                                                                                 cAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAg                                     <  2:83870/150‑1 (MQ=255)
                                                                                                                 cAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAg                                     >  2:883887/1‑150 (MQ=255)
                                                                                                                  aaTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAgc                                    >  2:2144150/1‑150 (MQ=255)
                                                                                                                   aTCGGAGTCGCCAAACAGCGGTTCAAACGCAGCCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAACGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAgcg                                   >  1:2103606/1‑150 (MQ=255)
                                                                                                                      ggAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCCGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTg                                >  2:139222/1‑150 (MQ=255)
                                                                                                                        aGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAACGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAg                              <  2:264801/150‑1 (MQ=255)
                                                                                                                         gTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGCGGTGCGTTGAATAAAGCGCTgcgc                             >  1:1330948/1‑150 (MQ=255)
                                                                                                                              cAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAt                        <  2:1129125/150‑1 (MQ=255)
                                                                                                                               aaaCAGCGGTTCATACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCTTTTTCTGCCGAAAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATc                       <  1:1991900/150‑1 (MQ=255)
                                                                                                                                aaCAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCg                      >  2:1016739/1‑150 (MQ=255)
                                                                                                                                  cAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCACAGGTGAGTTGAATAAAGCGCTGAGCCTGATCGGa                    >  2:109535/1‑150 (MQ=255)
                                                                                                                                    gCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTAGTCCACGCGGGGAAAGCAGACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAAAAAAGCGCTGAGCCTGATCGGatc                  <  1:935255/150‑3 (MQ=255)
                                                                                                                                       gTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCa               <  1:820020/150‑1 (MQ=255)
                                                                                                                                       cgagaaaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCa               >  1:179861/5‑150 (MQ=255)
                                                                                                                                         agaaaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGa             >  2:948023/3‑150 (MQ=255)
                                                                                                                                          cAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAt            <  2:2093206/150‑1 (MQ=255)
                                                                                                                                            aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa          >  2:1607660/1‑150 (MQ=255)
                                                                                                                                            aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa          >  1:527042/1‑150 (MQ=255)
                                                                                                                                            aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa          >  1:2160400/1‑150 (MQ=255)
                                                                                                                                            aaCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCTGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa          >  2:591805/1‑150 (MQ=255)
                                                                                                                                                gaGGAACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGAACTCCGTTTTCTGCAGATAAGCGATTTTCGGGGATTTACTTCAGCTCTTTCATCACATCAGAGGTGCGATGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt      <  1:1948033/149‑1 (MQ=255)
                                                                                                                                                cAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt      <  2:336004/150‑1 (MQ=255)
                                                                                                                                                cAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt      <  2:844580/150‑1 (MQ=255)
                                                                                                                                                cAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt      <  1:921111/150‑1 (MQ=255)
                                                                                                                                                  ggCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAACGCACTCCGTTTTCTGCCGAGAAGCGATTTTCGGCGATGTACTGCAGCTCTTTCCGCACAGCGGAGGTGCGTTGAAGAAAGCGCTGCGCCGGCTCGGATCTCAGAGTATAGTcc    >  2:327775/1‑150 (MQ=255)
                                                                                                                                                  ggCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAGCGGAACTCAGATTAAATTcc    <  1:148376/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTG  >  NC_000913/2319432‑2319729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: