Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,319,582 C→G 6.3% V99L (GTG→CTG) ‡ rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,319,5820CG 6.3% 466.0 / 15.8 139V99L (GTG→CTG) ‡rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (63/67);  new base G (6/3);  total (69/70)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 144 total aligned reads displayed.

ATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGAC  >  NC_000913/2319434‑2319731
                                                                                                                                                    |                                                                                                                                                     
aTTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGACAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGcaca                                                                                                                                                      <  1:1065742/150‑1 (MQ=255)
    aaaaTACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAACCCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATccc                                                                                                                                                  >  2:2197663/1‑149 (MQ=255)
    aaaaTACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCg                                                                                                                                                  >  2:1611883/1‑150 (MQ=255)
     aaaTACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGTTTCAAACGCAGGCACATCCGc                                                                                                                                                 >  1:1129125/1‑150 (MQ=255)
          cccGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCg                                                                                                                                            >  1:1040932/1‑150 (MQ=255)
           ccGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGt                                                                                                                                           >  2:567728/1‑150 (MQ=255)
            cGGTTTAGATCGTAAGCCGCAGTAAAATTATCGCGCCAGTAGCGCATAAACCACGCCTATGACTCCTGAGAACCTCGCCAGTTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGtt                                                                                                                                          <  2:1345324/150‑1 (MQ=255)
            cGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCGGATGTGCCTGCGtt                                                                                                                                          <  2:475173/150‑1 (MQ=255)
            cGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCGGATGTGCCTGCGtt                                                                                                                                          <  2:1509036/150‑1 (MQ=255)
              gTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtc                                                                                                                                        <  1:460079/150‑1 (MQ=255)
              gTTTAGATCGTAAGCCGAAGAAAAAATATCGCGCCAGTAGCGCATAAACCACGCCAGTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtc                                                                                                                                        <  1:2081239/150‑1 (MQ=255)
               tttAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATTACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtct                                                                                                                                       <  1:1637385/150‑1 (MQ=255)
                  aGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGt                                                                                                                                    >  1:2112797/1‑150 (MQ=255)
                   gATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTc                                                                                                                                   <  2:1530669/150‑1 (MQ=255)
                   gATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTAGTc                                                                                                                                   >  1:118928/1‑150 (MQ=255)
                     tCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCa                                                                                                                                 <  1:2150734/150‑1 (MQ=255)
                     tCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCa                                                                                                                                 >  1:1952648/1‑150 (MQ=255)
                           gCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCggg                                                                                                                           >  1:844580/1‑150 (MQ=255)
                           gCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCggg                                                                                                                           <  2:1652072/150‑1 (MQ=255)
                           gCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCggg                                                                                                                           >  1:11404/1‑150 (MQ=255)
                               cAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGaaa                                                                                                                       >  2:1184829/1‑150 (MQ=255)
                                        tATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCATGCGTTTCTCGTCCACGCGGGGAAAGCACACCGt                                                                                                              >  2:1280142/1‑150 (MQ=255)
                                          tCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGttt                                                                                                            >  1:774943/1‑150 (MQ=255)
                                          tCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGttt                                                                                                            <  2:2195721/150‑1 (MQ=255)
                                          tCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGGCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGttt                                                                                                            <  1:1828553/150‑1 (MQ=255)
                                           cgcgCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCCCGCGGGGAAAGCACACCGtttt                                                                                                           >  1:1304604/1‑150 (MQ=255)
                                             cgcCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCCCGCGGGGAAAGCACACCGTTTTCt                                                                                                         >  2:1986629/1‑150 (MQ=255)
                                             cgcCAGTAGCGCATAAACCACGACAATGCCTCCAGAGAACCTCGCCAGTTGTTACTCATTGCGGAACAAGCGGAGTCGGCCAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCt                                                                                                         <  2:1495999/150‑1 (MQ=255)
                                              gcCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTg                                                                                                        <  2:2112797/150‑1 (MQ=255)
                                                   tAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGAt                                                                                                   <  1:1343508/150‑1 (MQ=255)
                                                   tAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGAt                                                                                                   >  2:2119/1‑150 (MQ=255)
                                                       gcATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCTTTGCGGAACACTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACCTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACCCCGTTTTCTGCCGATAAGc                                                                                               >  2:766433/1‑150 (MQ=255)
                                                       gcATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGc                                                                                               <  1:1757275/150‑1 (MQ=255)
                                                       gcATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGc                                                                                               <  2:2047823/150‑1 (MQ=255)
                                                       gcATAAACCACGCCAATGACTACAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGc                                                                                               <  1:1380515/150‑1 (MQ=255)
                                                       gcATAAACACCGCCACTGACTCCAGCGAACCTCGCCAGGTGTAACGCATAGCGGAACAATCGGAGTCGGAAAACAGCGGGTCAAACTCAGGCACATCCGCCGGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGc                                                                                               <  2:565421/150‑1 (MQ=255)
                                                        cATAAACCACGCCAATGCCTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGGGTTTCTCGTCAACGCGGGGAACGCACACCGTTTTCTGCCGATAAGCg                                                                                              >  2:580977/1‑150 (MQ=255)
                                                        cATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCg                                                                                              >  2:1439446/1‑150 (MQ=255)
                                                            aaCCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGAttt                                                                                          <  1:184943/150‑1 (MQ=255)
                                                                   ccAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGGCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGa                                                                                   <  2:498237/150‑1 (MQ=255)
                                                                     aaTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACTCTGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATg                                                                                 <  1:171546/150‑1 (MQ=255)
                                                                     aaTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATg                                                                                 <  2:774943/150‑1 (MQ=255)
                                                                     aaTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATg                                                                                 >  2:1951342/1‑150 (MQ=255)
                                                                      aTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCTATTTTCGGCGATGt                                                                                >  2:188052/1‑150 (MQ=255)
                                                                        gACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTAc                                                                              <  2:1906903/150‑1 (MQ=255)
                                                                           tCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTc                                                                           <  1:235376/150‑1 (MQ=255)
                                                                            ccAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAACGCACACGGTGTTCTGCCGATACGCGATTTTCGGCGATGTGCTTCa                                                                          >  1:336004/1‑150 (MQ=255)
                                                                            ccAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCa                                                                          <  1:828981/150‑1 (MQ=255)
                                                                            ccAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCa                                                                          >  1:1137603/1‑150 (MQ=255)
                                                                            ccAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGAACTTCa                                                                          >  1:2029463/1‑150 (MQ=255)
                                                                             cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                         <  2:684457/150‑1 (MQ=255)
                                                                             cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                         >  1:732683/1‑150 (MQ=255)
                                                                             cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                         >  2:1211990/1‑150 (MQ=255)
                                                                             cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACAACGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                         >  2:1414867/1‑150 (MQ=255)
                                                                              agagAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGc                                                                        <  2:967043/150‑1 (MQ=255)
                                                                                   aCCTCGCCAGGTGTTACTCAATGCGGAACAATAGGAGTCGGCAAACAGCGGTTCAAACTCCGGCACATCCGCATGCGTTTCTCGTCCACGCGGGGTAAGCACACCGTTTTCTGCCGATAAGCGATTTTAGGCGATGTACTAAAGCTCttt                                                                   >  2:1691659/1‑150 (MQ=255)
                                                                                    ccTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTtc                                                                  <  2:231068/150‑1 (MQ=255)
                                                                                    ccTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTtc                                                                  >  2:1886032/1‑150 (MQ=255)
                                                                                     cTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTtca                                                                 <  1:1225638/150‑1 (MQ=255)
                                                                                     cTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTtca                                                                 <  1:562518/150‑1 (MQ=255)
                                                                                          cAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTcatcaca                                                            <  1:21147/150‑1 (MQ=255)
                                                                                            ggTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTcatcacatc                                                          <  2:182497/150‑1 (MQ=255)
                                                                                                 tACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCCCGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAgg                                                     >  2:917196/1‑150 (MQ=255)
                                                                                                 tACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAgg                                                     <  1:513475/150‑1 (MQ=255)
                                                                                                  aCTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGa                                                                                   <  1:605178/119‑1 (MQ=255)
                                                                                                   cTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAGGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCTATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTg                                                   <  1:2195455/150‑1 (MQ=255)
                                                                                                     cATTGCGGAACAATCGGAGTCGGCAACCAGCGGTTCAAACGCAGGCACATCCGCCTGCGATTCTCGTCCACGCGGGGCAAGCACACCGTTTTCTCGCGATAAGCGATTTTCGGCGATGTACTTCGGCTCTTGCATCACATCAGAGGTGCg                                                 >  2:372032/1‑150 (MQ=255)
                                                                                                       ttGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGtt                                               >  2:52016/1‑150 (MQ=255)
                                                                                                         gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGa                                             >  1:483624/1‑150 (MQ=255)
                                                                                                          cGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTAAGCTCTTTCATCACATCATAGGTGCGTTGaa                                            >  2:437896/1‑150 (MQ=255)
                                                                                                           ggAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAAt                                           <  1:1611883/150‑1 (MQ=255)
                                                                                                              aCAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATaaa                                        <  2:1700036/150‑1 (MQ=255)
                                                                                                               cAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAg                                       <  2:83870/150‑1 (MQ=255)
                                                                                                               cAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAg                                       >  2:883887/1‑150 (MQ=255)
                                                                                                                aaTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAgc                                      >  2:2144150/1‑150 (MQ=255)
                                                                                                                    ggAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCCGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTg                                  >  2:139222/1‑150 (MQ=255)
                                                                                                                      aGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAACGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAg                                <  2:264801/150‑1 (MQ=255)
                                                                                                                       gTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGCGGTGCGTTGAATAAAGCGCTgcgc                               >  1:1330948/1‑150 (MQ=255)
                                                                                                                            cAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAt                          <  2:1129125/150‑1 (MQ=255)
                                                                                                                             aaaCAGCGGTTCATACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCTTTTTCTGCCGAAAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATc                         <  1:1991900/150‑1 (MQ=255)
                                                                                                                              aaCAGCGGTTCAAACGCAGGCTCATCCGCCTGCTTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATAATCGGCGAGGTACTTCAGCTCTTTAATCACATCAGAGGTGAGTTGAATAAAGAGCTGAGAGTGATCg                        >  1:93600/1‑150 (MQ=255)
                                                                                                                              aaCAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCg                        >  2:1016739/1‑150 (MQ=255)
                                                                                                                                cAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCACAGGTGAGTTGAATAAAGCGCTGAGCCTGATCGGa                      >  2:109535/1‑150 (MQ=255)
                                                                                                                                  gCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAAc                    <  2:1040932/150‑1 (MQ=255)
                                                                                                                                  gCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTAGTCCACGCGGGGAAAGCAGACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAAAAAAGCGCTGAGCCTGATCGGatc                    <  1:935255/150‑3 (MQ=255)
                                                                                                                                     gTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCa                 <  1:820020/150‑1 (MQ=255)
                                                                                                                                     cgagaaaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCa                 >  1:179861/5‑150 (MQ=255)
                                                                                                                                       agaaaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGa               >  2:948023/3‑150 (MQ=255)
                                                                                                                                        cAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAt              <  2:2093206/150‑1 (MQ=255)
                                                                                                                                          aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa            >  2:1607660/1‑150 (MQ=255)
                                                                                                                                          aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa            >  1:527042/1‑150 (MQ=255)
                                                                                                                                          aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGAGTAGCGATTTTAGGCGATGTACTTCAGCGCTTTCATCACATAAGTGGTGAGTTGAATAAAGCGCTGAGACTTATGGGAACTCAGATTa            >  1:201799/1‑150 (MQ=255)
                                                                                                                                          aaCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCTGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa            >  2:591805/1‑150 (MQ=255)
                                                                                                                                            cGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAAAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTaaa          >  1:650315/1‑150 (MQ=255)
                                                                                                                                              gaGGAACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGAACTCCGTTTTCTGCAGATAAGCGATTTTCGGGGATTTACTTCAGCTCTTTCATCACATCAGAGGTGCGATGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt        <  1:1948033/149‑1 (MQ=255)
                                                                                                                                              cAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt        <  2:336004/150‑1 (MQ=255)
                                                                                                                                              cAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt        <  2:844580/150‑1 (MQ=255)
                                                                                                                                                ggCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAACGCACTCCGTTTTCTGCCGAGAAGCGATTTTCGGCGATGTACTGCAGCTCTTTCCGCACAGCGGAGGTGCGTTGAAGAAAGCGCTGCGCCGGCTCGGATCTCAGAGTATAGTcc      >  2:327775/1‑150 (MQ=255)
                                                                                                                                                ggCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAGCGGAACTCAGATTAAATTcc      <  1:148376/150‑1 (MQ=255)
                                                                                                                                                  cacaTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCTCATCAGAGTTGCGTTGTATACAGCGCTGAGCCTGATCGGATCTCAGATTAAATTCCTg    >  1:356186/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGAC  >  NC_000913/2319434‑2319731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: