Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,323,283 G→C 6.7% intergenic (+32/‑164) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,323,2830GC 6.7% 509.5 / 23.1 164intergenic (+32/‑164)atoC/atoDfused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (69/84);  new base C (8/3);  total (77/87)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.12e-01

Only 100 of 165 total aligned reads displayed.

GGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTAT  >  NC_000913/2323134‑2323430
                                                                                                                                                     |                                                                                                                                                   
ggAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAg                                                                                                                                                     >  2:414017/1‑150 (MQ=255)
   aGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgc                                                                                                                                                  >  2:127393/1‑150 (MQ=255)
   aGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgc                                                                                                                                                  <  2:627200/150‑1 (MQ=255)
    gTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgcg                                                                                                                                                 <  1:799952/150‑1 (MQ=255)
     tGCTGGAACAACCAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTAGAAACTCCCGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgcgc                                                                                                                                                <  2:907320/150‑1 (MQ=255)
      gCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGTATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCa                                                                                                                                               >  2:24421/1‑150 (MQ=255)
       cTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCaa                                                                                                                                              <  1:2113984/150‑1 (MQ=255)
         ggAACAACAAGAAGGAAACCGAACCCTCACTGCTTTAATGCTGGGCATCAGTCGCAGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAtt                                                                                                                                            <  1:292618/150‑1 (MQ=255)
             caacaaGAAGGAATCCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCt                                                                                                                                        <  2:566599/150‑1 (MQ=255)
              aacaaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                                                                       >  2:877443/1‑150 (MQ=255)
               acaaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGc                                                                                                                                      >  1:2021436/1‑150 (MQ=255)
                caaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCa                                                                                                                                     <  1:229581/150‑1 (MQ=255)
                caaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGAACCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACCGTGCGCAATTTTCTGCa                                                                                                                                     >  1:1446623/1‑150 (MQ=255)
                   gaagGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAg                                                                                                                                  <  2:2085192/150‑1 (MQ=39)
                    aagGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGc                                                                                                                                 <  2:2154547/150‑1 (MQ=255)
                     agGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGcc                                                                                                                                >  1:422416/1‑150 (MQ=255)
                       gAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGc                                                                                                                              >  2:2079546/1‑150 (MQ=255)
                       gAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAAGACGGTATCGATCCGGCGGAGGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATGTTCTTCATAGCCGc                                                                                                                              >  1:322393/1‑150 (MQ=255)
                        aaaCCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCt                                                                                                                             <  2:1122494/150‑1 (MQ=255)
                        aaaCCGAACCCGAACTGCTTTAATGCTGGGCATAAGACGCCGTGAATAGAACTATAAACTCCAGGAATACGGTATCCATCCGGCGGATGTATAACACAAAAACTTCCTATGCAGAAATATGCACAGTGAGCATATTTCTGCATAGACGca                                                                                                                             >  2:1973524/1‑149 (MQ=255)
                           ccGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCAt                                                                                                                          >  2:377834/1‑150 (MQ=255)
                             gAACCCGCACTGCTTTAATGCTTGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATCCGGTATCGATCCGGCGGATGTATAACACCAACCCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtc                                                                                                                        <  1:322809/150‑1 (MQ=255)
                              caCCCGCACTGCTTAAATGCTGGGCATCAGTCTCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtct                                                                                                                       <  2:337285/149‑1 (MQ=255)
                              aaCCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGGGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtct                                                                                                                       <  2:1868839/150‑1 (MQ=255)
                                  cGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATATGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCtt                                                                                                                   <  2:1362505/150‑1 (MQ=255)
                                    cACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtat                                                                                                                 >  1:1232257/1‑150 (MQ=255)
                                     aCTGCTTTAATGCGGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                                                                <  2:1304391/150‑1 (MQ=255)
                                       tGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATaaa                                                                                                              <  1:1689087/150‑1 (MQ=255)
                                           ttAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                                                          <  1:206908/150‑1 (MQ=255)
                                           ttAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                                                          <  1:441015/150‑1 (MQ=255)
                                            tAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATcccc                                                                                                         >  2:944361/1‑150 (MQ=255)
                                             aaTGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCa                                                                                                        <  2:1788390/150‑1 (MQ=255)
                                              aTGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACCCCAAAACTTGCTATGAAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTAATTCTCCTTATAAATCCCCAt                                                                                                       >  2:1616063/1‑150 (MQ=255)
                                              aTGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCAt                                                                                                       >  2:1017065/1‑150 (MQ=255)
                                                    ggCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAtt                                                                                                 >  1:1262316/1‑150 (MQ=255)
                                                    ggCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAtt                                                                                                 <  1:414017/150‑1 (MQ=255)
                                                      cATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTa                                                                                               >  1:173873/1‑150 (MQ=255)
                                                      cATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTa                                                                                               <  2:461701/150‑1 (MQ=255)
                                                      cATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTa                                                                                               <  2:774519/150‑1 (MQ=255)
                                                           gTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCt                                                                                          >  1:925785/1‑150 (MQ=255)
                                                            tCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCtt                                                                                         <  1:1309124/150‑1 (MQ=255)
                                                             cGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTc                                                                                        <  2:1810098/150‑1 (MQ=255)
                                                                 gTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTACttat                                                                                    >  1:1045129/1‑150 (MQ=255)
                                                                   gCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATAtta                                                                                  >  2:709755/1‑149 (MQ=255)
                                                                    cATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCa                                                                                 >  2:1761797/1‑150 (MQ=255)
                                                                     aTTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACATCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCaa                                                                                <  1:2040458/150‑1 (MQ=255)
                                                                       tGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCAAATTCAAtt                                                                              >  1:1198275/1‑150 (MQ=255)
                                                                           gTATAAACTCCATTAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGTATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                                                          <  2:1261443/150‑1 (MQ=255)
                                                                           gTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                                                          >  1:263203/1‑150 (MQ=255)
                                                                              taAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTaaa                                                                       <  2:2142242/150‑1 (MQ=255)
                                                                               aaaCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAAt                                                                      <  2:199320/150‑1 (MQ=255)
                                                                                 aCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATaa                                                                    <  2:1397148/150‑1 (MQ=255)
                                                                                  cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATaaa                                                                   >  1:743678/1‑149 (MQ=255)
                                                                                   tCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                                                                  <  1:625593/150‑1 (MQ=255)
                                                                                    ccAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                                                                 <  1:495516/150‑1 (MQ=255)
                                                                                      aGGAATACGGTTTCGATCAGGCTGATGTATAACACCAAAACTTGCTATTCAGAAATTTGCACAGTGCTCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaaa                                                               <  1:92959/150‑1 (MQ=255)
                                                                                       ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAAt                                                              >  1:411002/1‑150 (MQ=255)
                                                                                            aCGGTATCGATCCGGCGGATGTATAACACCAAAACTTGTTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaat                                                         >  2:1549622/1‑150 (MQ=255)
                                                                                              ggTATAGATCAGGAGGATGTATATCACCAAAACTTGCTATGCATAAATTTGCACAGTGCGCAATTTTCTGCATAGCCTCTCATTCTCCTTATAAATCCACATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaataa                                                       <  1:107577/150‑1 (MQ=255)
                                                                                                tATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtc                                                     <  2:1343672/150‑1 (MQ=255)
                                                                                                 ttCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtct                                                    <  2:687418/149‑1 (MQ=255)
                                                                                                 aTCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtct                                                    <  2:1232257/150‑1 (MQ=255)
                                                                                                   cGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCa                                                  >  2:1636855/1‑150 (MQ=255)
                                                                                                   cGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCACAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAATAATCAATTAATATTCAATTAGATAAATAACTAAATACAATAATGTCa                                                  >  2:1474372/1‑150 (MQ=255)
                                                                                                    gATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCAt                                                 >  2:232908/1‑150 (MQ=255)
                                                                                                    gATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCAt                                                 >  1:4860/1‑150 (MQ=255)
                                                                                                     aTCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCAtt                                                >  1:214675/1‑150 (MQ=255)
                                                                                                         ggcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGAAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTgg                                            <  1:1560566/150‑1 (MQ=255)
                                                                                                          gcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc                                           <  2:415985/150‑1 (MQ=255)
                                                                                                          gcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc                                           <  2:1092413/150‑1 (MQ=255)
                                                                                                           cggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTAATTCTGGCa                                          >  1:2007349/1‑150 (MQ=255)
                                                                                                             gATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACt                                        >  1:1287802/1‑150 (MQ=255)
                                                                                                               tGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTcc                                      <  1:48272/150‑1 (MQ=255)
                                                                                                                    aaCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCTCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCACATTCTGGAACTCCCCTTg                                 >  2:1389479/1‑150 (MQ=255)
                                                                                                                    aaCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTg                                 <  2:1133095/150‑1 (MQ=255)
                                                                                                                     acacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCACTCATTCTCATTATAAATCACCAACCAATATAAAACTAAAAAATCAAATAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGc                                >  2:1471611/1‑150 (MQ=255)
                                                                                                                     acacCAAAACTTGCTATGAAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCACCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGc                                <  2:322393/150‑1 (MQ=255)
                                                                                                                      cacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTGCATATTCAATTAGTTAAATAACTAAATCCATTAATCTCATTCTGGAACTCGCCTTGCt                               >  1:1451555/1‑150 (MQ=255)
                                                                                                                      cacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCAGTCTCCTTATAAATCCCCTTCCAATTTTTCCCTTCATATTCAATTAGTTAAATAACGTAAGCCAAGAATCTCAGTCTGGCACTCCCCTTGCt                               >  1:200706/1‑150 (MQ=255)
                                                                                                                        ccAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTAt                             >  1:868946/1‑150 (MQ=255)
                                                                                                                        ccAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCGTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATCCTGGCACTCCCCTTGCTAt                             >  1:2112521/1‑150 (MQ=255)
                                                                                                                         cAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTAtt                            >  2:790417/1‑150 (MQ=255)
                                                                                                                         cAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTAtt                            <  2:352545/150‑1 (MQ=255)
                                                                                                                            aaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGcc                         <  2:706218/150‑1 (MQ=255)
                                                                                                                            aaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGcc                         <  2:448973/150‑1 (MQ=255)
                                                                                                                              cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTg                       <  2:1512180/150‑1 (MQ=255)
                                                                                                                               ttGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGa                      <  2:1232033/150‑1 (MQ=255)
                                                                                                                                tGCTATGCAGAAATTTTCACAGTGCGAAATTTTCTGCATAGCCGCTCATTCTCCTTATATATCCCCATACAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                     <  2:956473/150‑1 (MQ=255)
                                                                                                                                tGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAATTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                     <  1:1327986/150‑1 (MQ=255)
                                                                                                                                 gCTATGCAGAAATTTGCACAGTGAGCAATTTTCTGTATAGCAGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACt                    <  1:373929/150‑1 (MQ=255)
                                                                                                                                 gCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACt                    >  1:1371392/1‑150 (MQ=38)
                                                                                                                                   tATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCCTGCTATTGCCTGACTGt                  >  2:504901/1‑150 (MQ=39)
                                                                                                                                     tGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCATTTAGTTAAATAACTAAATCCAAAAATCTCATACTGGCACTCCCAGTGCTTGTGCCTGACTGTAc                >  1:104189/1‑150 (MQ=37)
                                                                                                                                     tGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAc                >  1:415759/1‑150 (MQ=39)
                                                                                                                                       cAGAAAATTGCGCACTGTGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAccc              >  2:563784/1‑150 (MQ=255)
                                                                                                                                       cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAccc              >  1:72200/1‑150 (MQ=255)
                                                                                                                                            aTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAAc         <  1:790417/150‑1 (MQ=255)
                                                                                                                                                gCACAGTGCGCAATTTTCTGCATAGCCGCTCCTTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATCTTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGgtg     <  1:1859298/150‑1 (MQ=255)
                                                                                                                                                  acaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTa   <  1:105435/150‑1 (MQ=255)
                                                                                                                                                  acaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTa   >  2:1934943/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTAT  >  NC_000913/2323134‑2323430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: