Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,323,291 T→A 6.7% intergenic (+40/‑156) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,323,2910TA 6.7% 468.3 / 16.4 158intergenic (+40/‑156)atoC/atoDfused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (63/84);  new base A (8/3);  total (71/87)
Fisher's exact test for biased strand distribution p-value = 6.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

Only 100 of 164 total aligned reads displayed.

TGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGA  >  NC_000913/2323142‑2323440
                                                                                                                                                     |                                                                                                                                                     
 ggAACAACAAGAAGGAAACCGAACCCTCACTGCTTTAATGCTGGGCATCAGTCGCAGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAtt                                                                                                                                                      <  1:292618/150‑1 (MQ=255)
 ggAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGGATAAACTCCAGGAATACGGTATCGATCCGGCGGCTGGATAACCCCAAAACTTGCTATGCAGAAATTTGCACAGTGCTCAAtt                                                                                                                                                      >  2:322809/1‑150 (MQ=255)
   aacaacAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGAATCAGTCGCCGTGCATAGATGTATAAACACCAGGAATACGGTATCGATCCGGCGGATGTAAAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAtttt                                                                                                                                                    >  2:2002621/1‑150 (MQ=255)
     caacaaGAAGGAATCCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCt                                                                                                                                                  <  2:566599/150‑1 (MQ=255)
     caacaaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCt                                                                                                                                                  >  2:1208358/1‑150 (MQ=255)
       acaaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGc                                                                                                                                                <  2:533318/150‑1 (MQ=255)
        caaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCa                                                                                                                                               >  2:841097/1‑150 (MQ=255)
        caaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGAACCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACCGTGCGCAATTTTCTGCa                                                                                                                                               >  1:1446623/1‑150 (MQ=255)
         aagaagGACACCGAACCCGCACTGCTTTAATGCTCGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAATCTTGCTATGCAGATAATTGCGCACTGTGCAAATTTCTGCAt                                                                                                                                              <  1:1983843/150‑1 (MQ=255)
         aagaagGAAACCGACCCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCAt                                                                                                                                              <  2:1395490/150‑1 (MQ=39)
         aagaagGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTAGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCAt                                                                                                                                              <  2:1911082/150‑1 (MQ=255)
           gaagGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAg                                                                                                                                            >  1:213032/1‑150 (MQ=255)
           gaagGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAg                                                                                                                                            <  2:2085192/150‑1 (MQ=39)
            aagGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGc                                                                                                                                           <  2:2154547/150‑1 (MQ=255)
             agGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGcc                                                                                                                                          >  1:422416/1‑150 (MQ=255)
              ggAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCg                                                                                                                                         >  2:1124172/1‑150 (MQ=255)
               gAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAAGACGGTATCGATCCGGCGGAGGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATGTTCTTCATAGCCGc                                                                                                                                        >  1:322393/1‑150 (MQ=255)
                aaaCCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCt                                                                                                                                       <  2:1122494/150‑1 (MQ=255)
                aaaCCGAACCCGAACTGCTTTAATGCTGGGCATAAGACGCCGTGAATAGAACTATAAACTCCAGGAATACGGTATCCATCCGGCGGATGTATAACACAAAAACTTCCTATGCAGAAATATGCACAGTGAGCATATTTCTGCATAGACGca                                                                                                                                       >  2:1973524/1‑149 (MQ=255)
                  aCCGAACCAGCAGTGCTTTAATGCGGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCACGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCa                                                                                                                                     <  2:178971/150‑1 (MQ=255)
                   ccGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCAt                                                                                                                                    >  2:377834/1‑150 (MQ=255)
                     gAACCCGCACTGCTTTAATGCTTGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATCCGGTATCGATCCGGCGGATGTATAACACCAACCCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtc                                                                                                                                  <  1:322809/150‑1 (MQ=255)
                      aaCCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGGGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtct                                                                                                                                 <  2:1868839/150‑1 (MQ=255)
                            cACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtat                                                                                                                           >  1:1232257/1‑150 (MQ=255)
                             aCTGCTTTAATGCGGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                                                                          <  2:1304391/150‑1 (MQ=255)
                               tGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATaaa                                                                                                                        <  1:1689087/150‑1 (MQ=255)
                                   ttAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                                                                    <  1:206908/150‑1 (MQ=255)
                                   ttAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                                                                    <  1:441015/150‑1 (MQ=255)
                                    tAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATcccc                                                                                                                   >  2:944361/1‑150 (MQ=255)
                                      aTGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCAt                                                                                                                 >  2:1017065/1‑150 (MQ=255)
                                            ggCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAtt                                                                                                           >  1:1446334/1‑150 (MQ=255)
                                            ggCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAtt                                                                                                           <  1:414017/150‑1 (MQ=255)
                                            ggCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAtt                                                                                                           >  1:1262316/1‑150 (MQ=255)
                                             gCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGGCGCTCATTCTCCTTATAAATc                                                                                                                      <  1:2081848/138‑1 (MQ=255)
                                             gCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGGCGCTCATTCTCCTTATAAATc                                                                                                                      >  2:2081848/1‑138 (MQ=255)
                                              cATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTa                                                                                                         >  2:1511622/1‑150 (MQ=255)
                                              cATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTa                                                                                                         <  2:461701/150‑1 (MQ=255)
                                              cATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTa                                                                                                         <  2:774519/150‑1 (MQ=255)
                                                  aGTCGCCGTGCATTGATGTATAAACTCCAGGAATACTGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATccc                                                                                                     <  1:483385/150‑1 (MQ=255)
                                                    tCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCtt                                                                                                   <  1:1309124/150‑1 (MQ=255)
                                                         gTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTACttat                                                                                              >  1:1045129/1‑150 (MQ=255)
                                                          tGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATAtt                                                                                             <  2:706917/150‑1 (MQ=255)
                                                           gCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTc                                                                                            <  2:897819/150‑1 (MQ=255)
                                                            cATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCa                                                                                           >  2:1761797/1‑150 (MQ=255)
                                                               tGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCAAATTCAAtt                                                                                        >  1:1198275/1‑150 (MQ=255)
                                                                taTGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAAATTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTa                                                                                       <  1:850637/149‑1 (MQ=255)
                                                                gATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAACTa                                                                                       >  2:1322526/1‑150 (MQ=255)
                                                                   gTATAAACTCCATTAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGTATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                                                                    <  2:1261443/150‑1 (MQ=255)
                                                                   gTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                                                                    >  1:263203/1‑150 (MQ=255)
                                                                     ataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCTCAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTaa                                                                                  <  1:2018004/150‑1 (MQ=255)
                                                                     ataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTaa                                                                                  <  1:1511622/150‑1 (MQ=255)
                                                                          cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATaaa                                                                             >  1:743678/1‑149 (MQ=255)
                                                                           tCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                                                                            <  1:625593/150‑1 (MQ=255)
                                                                            ccAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                                                                           <  1:495516/150‑1 (MQ=255)
                                                                            acAGGAATACTGTATCGATCCGGCGTATGTATAACACCAAAACTTGCTATGCAGAAATTTTCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                                                                           <  1:2030879/149‑1 (MQ=255)
                                                                              aGGAATACGGTTTCGATCAGGCTGATGTATAACACCAAAACTTGCTATTCAGAAATTTGCACAGTGCTCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaaa                                                                         <  1:92959/150‑1 (MQ=255)
                                                                              aGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaaa                                                                         <  1:707784/150‑1 (MQ=255)
                                                                               ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAAt                                                                        >  1:411002/1‑150 (MQ=255)
                                                                                    aCGGTATCGATCCGGCGGATGTATAACACCAAAACTTGTTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaat                                                                   >  2:1549622/1‑150 (MQ=255)
                                                                                      ggTATAGATCAGGAGGATGTATATCACCAAAACTTGCTATGCATAAATTTGCACAGTGCGCAATTTTCTGCATAGCCTCTCATTCTCCTTATAAATCCACATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaataa                                                                 <  1:107577/150‑1 (MQ=255)
                                                                                         ttCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtct                                                              <  2:687418/149‑1 (MQ=255)
                                                                                           cGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCACAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAATAATCAATTAATATTCAATTAGATAAATAACTAAATACAATAATGTCa                                                            >  2:1474372/1‑150 (MQ=255)
                                                                                            gATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCAt                                                           >  2:232908/1‑150 (MQ=255)
                                                                                            gATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCAt                                                           >  2:1608760/1‑150 (MQ=255)
                                                                                              tCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTCGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTc                                                         <  2:1625928/150‑1 (MQ=255)
                                                                                                 ggcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGAAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTgg                                                      <  1:1560566/150‑1 (MQ=255)
                                                                                                  gcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc                                                     <  2:415985/150‑1 (MQ=255)
                                                                                                   cggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTAATTCTGGCa                                                    >  1:2007349/1‑150 (MQ=255)
                                                                                                     gATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACt                                                  >  1:1287802/1‑150 (MQ=255)
                                                                                                       tGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTcc                                                <  1:48272/150‑1 (MQ=255)
                                                                                                           taACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGTCACTCCCCtt                                            >  2:723670/1‑150 (MQ=255)
                                                                                                            aaCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCTCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCACATTCTGGAACTCCCCTTg                                           >  2:1389479/1‑150 (MQ=255)
                                                                                                             acacCAAAACTTGCTATGAAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCACCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGc                                          <  2:322393/150‑1 (MQ=255)
                                                                                                              cacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCt                                         >  2:666585/1‑150 (MQ=255)
                                                                                                                ccAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTAATTCTGGCACTCCCCTTGCTAt                                       <  1:2179195/150‑1 (MQ=255)
                                                                                                                 cAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTAtt                                      <  2:352545/150‑1 (MQ=255)
                                                                                                                   aaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGc                                    <  1:1763754/150‑1 (MQ=255)
                                                                                                                   aaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGc                                    <  2:1769587/150‑1 (MQ=255)
                                                                                                                   aaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATACCCATCCAATTTATCCCTTCATATACAATTAGTTAAATAACTAAATCCAATAAACACATTCTGGCACTCCCCTTGCTATTGc                                    >  2:1355776/1‑150 (MQ=255)
                                                                                                                    aaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATACTCTCATTCTGGCACTCCCCTTGCTATTGcc                                   <  1:877443/150‑1 (MQ=255)
                                                                                                                    aaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGcc                                   <  2:448973/150‑1 (MQ=255)
                                                                                                                       ttGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGa                                <  2:1232033/150‑1 (MQ=255)
                                                                                                                        tGCTATGCAGAAATTTTCACAGTGCGAAATTTTCTGCATAGCCGCTCATTCTCCTTATATATCCCCATACAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                               <  2:956473/150‑1 (MQ=255)
                                                                                                                        tGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAATTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc                               <  1:1327986/150‑1 (MQ=255)
                                                                                                                         gCTATGCAGAAATTTGCACAGTGAGCAATTTTCTGTATAGCAGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACt                              <  1:373929/150‑1 (MQ=255)
                                                                                                                         gCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACt                              >  1:1371392/1‑150 (MQ=38)
                                                                                                                           tATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCCTGCTATTGCCTGACTGt                            >  2:504901/1‑150 (MQ=39)
                                                                                                                             tGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCATTTAGTTAAATAACTAAATCCAAAAATCTCATACTGGCACTCCCAGTGCTTGTGCCTGACTGTAc                          >  1:104189/1‑150 (MQ=37)
                                                                                                                             tGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAc                          >  1:415759/1‑150 (MQ=39)
                                                                                                                              gCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCGTTGCTATTGCCTGACTGTAcc                         >  2:1936280/1‑150 (MQ=255)
                                                                                                                              gCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCAGGCACTCCCATAGCTATTGCCTGACTGTAcc                         >  2:2146708/1‑150 (MQ=38)
                                                                                                                               cAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATACAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAccc                        <  2:1415856/150‑1 (MQ=255)
                                                                                                                                    aTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAAc                   <  1:790417/150‑1 (MQ=255)
                                                                                                                                        gCACAGTGCGCAATTTTCTGCATAGCCGCTCCTTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATCTTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGgtg               <  1:1859298/150‑1 (MQ=255)
                                                                                                                                         cacaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGgtgt              <  1:972515/150‑1 (MQ=255)
                                                                                                                                          acaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTa             <  1:105435/150‑1 (MQ=255)
                                                                                                                                                cgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAag       >  1:256514/1‑150 (MQ=255)
                                                                                                                                                cgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAag       <  2:411002/150‑1 (MQ=255)
                                                                                                                                                cgcAATTTTCTGCATAGCAGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTTCTATTGCCTGACTGTACCCACAACTGTGTATGCAag       <  2:1857204/150‑1 (MQ=255)
                                                                                                                                                    aTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAggg   <  1:2179543/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGA  >  NC_000913/2323142‑2323440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: