Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,356,734 G→C 7.6% intergenic (+23/‑170) glpC → / → yfaD anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,356,7340GC 7.6% 521.7 / 27.5 158intergenic (+23/‑170)glpC/yfaDanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base G (64/81);  new base C (5/7);  total (69/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

Only 100 of 159 total aligned reads displayed.

TAGAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAA  >  NC_000913/2356590‑2356883
                                                                                                                                                |                                                                                                                                                     
tAGAAGAAATCGGCGCAGATCTTGTGGTCACCGACTGCTAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTATTCGCTGCGAACATCCGATTACGATACTTGGCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCAtgt                                                                                                                                                  <  1:1431108/150‑1 (MQ=255)
  gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                >  2:569124/1‑150 (MQ=255)
  gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                <  2:1932986/150‑1 (MQ=255)
    agaaAGCGGCGCAGATCTGGTGGTCACCGACTACGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGtt                                                                                                                                              >  1:1119026/1‑150 (MQ=255)
        aGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCg                                                                                                                                          <  1:853734/150‑1 (MQ=255)
         ccggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCgg                                                                                                                                         <  1:764451/149‑1 (MQ=255)
          cggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATTTCCACAAGTCTTCTCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTTGCTTTAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCggg                                                                                                                                        <  2:331033/150‑1 (MQ=255)
          cggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCTCAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                                                                        <  1:1592432/150‑1 (MQ=255)
          cggcgCAGATCTGGTGGTCACCGACTGCGAAACCTCTAACTGCCAGATTGAGATGTCCACAAGTCCTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                                                                        <  2:132520/150‑1 (MQ=255)
           ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGc                                                                                                                                       <  2:1103922/150‑1 (MQ=255)
           ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGGCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGc                                                                                                                                       <  1:2131092/150‑1 (MQ=255)
              gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                    <  1:415823/150‑1 (MQ=255)
              gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                    <  2:1876625/150‑1 (MQ=255)
              gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                    >  1:1427791/1‑150 (MQ=255)
              gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGATTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                    <  1:1038665/150‑1 (MQ=255)
               cAGATCTGGTGGTCACCGACTGCGAAAGCTGTAAATGGCAGATTGTGATGTCCACAAGTCTTCGCTGCGAACATCAGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAttt                                                                                                                                   <  1:1854036/150‑1 (MQ=255)
                aGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtttt                                                                                                                                  <  2:1204540/150‑1 (MQ=255)
                  aTCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTg                                                                                                                                <  2:1869871/150‑1 (MQ=255)
                  aTCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGACATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTg                                                                                                                                <  2:2087707/150‑1 (MQ=255)
                   tCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGc                                                                                                                               >  2:1587764/1‑150 (MQ=255)
                    cTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCCGGCGCTGGCTTAAACTCCTTTCTGCTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                                                                              <  1:731365/150‑1 (MQ=255)
                        tggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTaca                                                                                                                          <  2:1697049/150‑1 (MQ=255)
                         ggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTacac                                                                                                                         <  1:242459/150‑1 (MQ=255)
                          gtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACg                                                                                                                        >  1:1644368/1‑150 (MQ=255)
                           tCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGAATGTGGTTACCGGGCATTTTTGCGTACACGa                                                                                                                       >  1:1829482/1‑150 (MQ=255)
                            cACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGAt                                                                                                                      >  1:740263/1‑150 (MQ=255)
                             aCCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTTCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGAtt                                                                                                                     >  2:1100836/1‑150 (MQ=255)
                              ccGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTc                                                                                                                    >  2:59856/1‑150 (MQ=255)
                               cGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTcc                                                                                                                   >  2:989650/1‑150 (MQ=255)
                                gACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCg                                                                                                                  >  2:469799/1‑150 (MQ=255)
                                   tGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                                                               <  2:1501334/150‑1 (MQ=255)
                                   ggCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTATCTGAGGCCCGTTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                                                               <  1:356824/149‑1 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGcc                                                                                                              <  1:510686/150‑1 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGcc                                                                                                              >  2:2087824/1‑150 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACAGGGCATTTTTGCGTACACGATTCCGTGcc                                                                                                              >  1:1313006/1‑150 (MQ=255)
                                           cTGTAAATGTCAGATTGAGAGGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGACGCCCGGTAAGCATTTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTa                                                                                                       <  1:266534/150‑1 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                     >  2:2022406/1‑150 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                     <  1:684745/150‑1 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                     >  2:119664/1‑150 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                     <  1:2107638/150‑1 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                     <  2:821028/150‑1 (MQ=255)
                                               aaaTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCg                                                                                                   >  2:1075345/1‑150 (MQ=255)
                                                 aTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGtt                                                                                                 >  1:292632/1‑150 (MQ=255)
                                                   ggCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGc                                                                                               <  1:724399/150‑1 (MQ=255)
                                                        aTTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGc                                                                                          <  2:239877/150‑1 (MQ=255)
                                                        aTTGAGATGTCCACAAGGCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGc                                                                                          <  1:1077236/150‑1 (MQ=255)
                                                         ttGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCa                                                                                         <  2:405823/150‑1 (MQ=255)
                                                          tGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAg                                                                                        <  1:806710/150‑1 (MQ=255)
                                                           gagaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGt                                                                                       <  1:840055/150‑1 (MQ=255)
                                                           gagaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGt                                                                                       >  2:1948955/1‑150 (MQ=255)
                                                                  ccACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGaagactt                                                                               >  2:568046/1‑145 (MQ=255)
                                                                   cacaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTc                                                                               >  2:718010/1‑150 (MQ=255)
                                                                      aaGTCTTCTCTGCGAACATCCGAGTACGCTACTGGCCCAGGCGCTGGCATAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                            <  1:924932/150‑1 (MQ=255)
                                                                      aaGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                            <  1:1082730/150‑1 (MQ=255)
                                                                      aaGTCATCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                            <  1:933057/150‑1 (MQ=255)
                                                                       aGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCGGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctct                                                                           <  1:234635/150‑1 (MQ=255)
                                                                        gTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTg                                                                          <  2:833114/150‑1 (MQ=255)
                                                                           ttCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTTGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGaaa                                                                       <  2:1158718/150‑1 (MQ=255)
                                                                             cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAAc                                                                     <  1:1912448/150‑1 (MQ=255)
                                                                             cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGGGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTGGCAACGCAGTGAAAATTCCTCTGAAAAc                                                                     <  1:1277663/150‑1 (MQ=255)
                                                                              gCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACg                                                                    >  2:1350174/1‑150 (MQ=255)
                                                                                tGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtc                                                                  <  2:1062031/150‑1 (MQ=255)
                                                                                    aaCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGc                                                              <  1:469799/150‑1 (MQ=255)
                                                                                     aCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa                                                             <  1:1141707/150‑1 (MQ=255)
                                                                                     aCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa                                                             <  1:1587764/150‑1 (MQ=255)
                                                                                      cATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGGGCCCAATGTATGCGTTGCCACGCAGTGAAAATTCCTCTGAAAACGTCTCGCaa                                                            >  2:1378076/1‑150 (MQ=255)
                                                                                        tCCGAATACGCTACTGGCCCAGGAGCTGTCAAAAACTACGTTCTGATGCCCCGAAAGCATCTGGTTACCGGGAATATTTGCGTACACGAATCCGTGCCCAAAGTATTCGTAGAAACGCAGTGATAATTCCTCTGAAAACGTCTCGCATAg                                                          >  2:1407702/1‑150 (MQ=255)
                                                                                          cGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGc                                                        <  1:2152854/150‑1 (MQ=255)
                                                                                          cGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGc                                                        <  1:1898678/150‑1 (MQ=255)
                                                                                           gATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCt                                                       >  1:186213/1‑150 (MQ=255)
                                                                                             ttACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGa                                                     <  2:1099460/150‑1 (MQ=255)
                                                                                                 gCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACt                                                 <  2:527206/150‑1 (MQ=255)
                                                                                                    aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc                                              >  2:1645107/1‑150 (MQ=255)
                                                                                                    aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc                                              <  2:1707031/150‑1 (MQ=255)
                                                                                                     cTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCa                                             >  2:493350/1‑150 (MQ=255)
                                                                                                       ggCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTTGCAGa                                           >  1:2133678/1‑150 (MQ=255)
                                                                                                               cgcTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAg                                   >  1:1737790/1‑150 (MQ=255)
                                                                                                               cgcTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAg                                   >  1:603363/1‑150 (MQ=255)
                                                                                                                 cTGGCTTAAACTCCTTTCTGCTGCCCGGTCAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGc                                 <  2:1938716/150‑1 (MQ=255)
                                                                                                                   ggCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCt                               >  2:2191351/1‑150 (MQ=255)
                                                                                                                     cTTAAACTCCTTTCTGATGCGCGGTTAGCATGTGGTTACCGGGTTTTTTTGAGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAATCGTCTCGCAAAGGCTTAAACTGGCAGATGTCAAAGGCCTgg                             <  2:772023/150‑1 (MQ=255)
                                                                                                                      ttAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTggg                            >  2:1771372/1‑150 (MQ=255)
                                                                                                                       tAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGa                           <  1:687851/150‑1 (MQ=255)
                                                                                                                        aaaCTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGAt                          >  2:2072485/1‑150 (MQ=255)
                                                                                                                              cTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGt                    <  1:1948955/150‑1 (MQ=255)
                                                                                                                              cTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGt                    >  2:2136092/1‑150 (MQ=255)
                                                                                                                                caaaaaTGCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCTAAGGCCTGGGATAACCGTaa                  >  2:220678/6‑150 (MQ=255)
                                                                                                                                    gATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTc              >  2:1025791/1‑150 (MQ=255)
                                                                                                                                     aTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcg             <  2:1091034/150‑1 (MQ=255)
                                                                                                                                      agCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgc            <  1:2193383/149‑1 (MQ=255)
                                                                                                                                       gCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGGTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg           <  1:1352019/150‑1 (MQ=255)
                                                                                                                                       gCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGGTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg           <  2:1119026/150‑1 (MQ=255)
                                                                                                                                       gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGTCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg           >  1:951239/1‑150 (MQ=255)
                                                                                                                                       gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg           >  2:2135575/1‑150 (MQ=255)
                                                                                                                                       acccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGAGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGATACTGGCAGCTGTCAAAGGCCTGGGATACCCGTAATGTcgcg           >  1:423049/2‑150 (MQ=255)
                                                                                                                                         ccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtc         >  1:1814712/1‑150 (MQ=255)
                                                                                                                                          cGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtca        <  1:1032501/150‑1 (MQ=255)
                                                                                                                                             tAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTAGCAAAGTCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatca     <  1:119664/150‑1 (MQ=255)
                                                                                                                                               aGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATa   >  1:1643578/1‑150 (MQ=255)
                                                                                                                                                gCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATaa  >  2:1680982/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TAGAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAA  >  NC_000913/2356590‑2356883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: