Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,356,737 T→C 7.6% intergenic (+26/‑167) glpC → / → yfaD anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,356,7370TC 7.6% 462.2 / 21.2 158intergenic (+26/‑167)glpC/yfaDanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base T (65/81);  new base C (5/7);  total (70/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

Only 100 of 162 total aligned reads displayed.

TAGAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATA  >  NC_000913/2356590‑2356886
                                                                                                                                                   |                                                                                                                                                     
tAGAAGAAATCGGCGCAGATCTTGTGGTCACCGACTGCTAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTATTCGCTGCGAACATCCGATTACGATACTTGGCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCAtgt                                                                                                                                                     <  1:1431108/150‑1 (MQ=255)
  gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                   <  1:1835391/150‑1 (MQ=255)
  gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                   >  2:569124/1‑150 (MQ=255)
    agaaAGCGGCGCAGATCTGGTGGTCACCGACTACGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGtt                                                                                                                                                 >  1:1119026/1‑150 (MQ=255)
        aGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCg                                                                                                                                             <  1:853734/150‑1 (MQ=255)
         gctgcgcAGATCTGGTGGTCACCTACTGCGAAACCTGTAAATGGCAGATTGTGATGTCCACATGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGATTAAACTCCTTTCTTATGCCCGGTAACCACATGCTTACCgg                                                                                                                                            <  1:868583/147‑1 (MQ=255)
         ccggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCgg                                                                                                                                            <  1:764451/149‑1 (MQ=255)
          cggcgCAGATCTGGTGGTCACCGACTGCGAAACCTCTAACTGCCAGATTGAGATGTCCACAAGTCCTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                                                                           <  2:132520/150‑1 (MQ=255)
           ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCCGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGc                                                                                                                                          <  1:1894920/150‑1 (MQ=255)
           ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGc                                                                                                                                          <  2:1103922/150‑1 (MQ=255)
              gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                       <  1:415823/150‑1 (MQ=255)
              gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGATTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                       <  1:1038665/150‑1 (MQ=255)
               cAGATCTGGTGGTCACCGACTGCGAAAGCTGTAAATGGCAGATTGTGATGTCCACAAGTCTTCGCTGCGAACATCAGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAttt                                                                                                                                      <  1:1854036/150‑1 (MQ=255)
                aGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtttt                                                                                                                                     <  2:1204540/150‑1 (MQ=255)
                  aTCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTg                                                                                                                                   <  2:1869871/150‑1 (MQ=255)
                    cTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCCGGCGCTGGCTTAAACTCCTTTCTGCTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                                                                                 <  1:731365/150‑1 (MQ=255)
                      ggtggtCACCGACTGCGAAACCTGTAAATGGCAGCTTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTa                                                                                                                               <  1:220249/150‑1 (MQ=255)
                      ggtggtCACCGACTGCGAAACCTGTAAATGCCAGATTGCGATGCCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTa                                                                                                                               <  1:213143/150‑1 (MQ=255)
                           tCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGa                                                                                                                          >  1:814227/1‑150 (MQ=255)
                             aCCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTTCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGAtt                                                                                                                        >  2:716419/1‑150 (MQ=255)
                              ccGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTc                                                                                                                       >  2:59856/1‑150 (MQ=255)
                               cGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTcc                                                                                                                      >  2:989650/1‑150 (MQ=255)
                               cGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTcc                                                                                                                      >  1:1171138/1‑150 (MQ=255)
                                gACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCg                                                                                                                     >  2:469799/1‑150 (MQ=255)
                                   tGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                                                                  <  1:625297/150‑1 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAGACTCCGTTCTGATGCCCGGTAAGCATGTGGTTCCCGGGCTTTATTGCGTACACGATTCCGTGcc                                                                                                                 >  1:321732/1‑150 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGcc                                                                                                                 >  2:2087824/1‑150 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACAGGGCATTTTTGCGTACACGATTCCGTGcc                                                                                                                 >  1:1313006/1‑150 (MQ=255)
                                     cGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGccc                                                                                                                <  2:1923010/150‑1 (MQ=255)
                                          ccTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGt                                                                                                           >  1:159779/1‑150 (MQ=255)
                                           cTGTAAATGTCAGATTGAGAGGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGACGCCCGGTAAGCATTTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTa                                                                                                          <  1:266534/150‑1 (MQ=255)
                                            tGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCATTGTAt                                                                                                         >  1:400882/1‑150 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                        <  2:821028/150‑1 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                        >  2:119664/1‑150 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                        >  2:2022406/1‑150 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                        <  1:684745/150‑1 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                        <  1:2107638/150‑1 (MQ=255)
                                                   ggCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTATGCTACTGGCCCAGGCGCTTGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGc                                                                                                  <  2:2080410/150‑1 (MQ=255)
                                                    gCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCa                                                                                                 <  2:1959933/150‑1 (MQ=255)
                                                       taTTGAGATGTCCACACGTCTTCGCTGCGAACATCCGATTACGCTACTTGCCGAGGCGCTGGCTTAAACTCCTTTCTTATGCGCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACg                                                                                              <  2:319501/149‑1 (MQ=255)
                                                        aTTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGc                                                                                             <  2:239877/150‑1 (MQ=255)
                                                        aTTGAGATGTCCACAAGGCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGc                                                                                             <  1:1077236/150‑1 (MQ=255)
                                                         ttGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCa                                                                                            <  2:405823/150‑1 (MQ=255)
                                                                 tCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTCAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                                                                    <  1:53939/150‑1 (MQ=255)
                                                                 tCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                                                                    <  1:733301/150‑1 (MQ=255)
                                                                  ccACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGaagactt                                                                                  >  2:568046/1‑145 (MQ=255)
                                                                   cacaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTc                                                                                  >  2:169412/1‑150 (MQ=255)
                                                                      aaGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                               <  1:1082730/150‑1 (MQ=255)
                                                                      aaGTCATCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                               <  1:933057/150‑1 (MQ=255)
                                                                       aGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCGGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctct                                                                              <  1:234635/150‑1 (MQ=255)
                                                                        gTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTg                                                                             <  2:833114/150‑1 (MQ=255)
                                                                         tCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGa                                                                            <  2:1566565/150‑1 (MQ=255)
                                                                           ttCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTTGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGaaa                                                                          <  2:1158718/150‑1 (MQ=255)
                                                                             cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAAc                                                                        <  1:1912448/150‑1 (MQ=255)
                                                                             cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGGGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTGGCAACGCAGTGAAAATTCCTCTGAAAAc                                                                        <  1:1277663/150‑1 (MQ=255)
                                                                              gCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACg                                                                       >  2:1350174/1‑150 (MQ=255)
                                                                                tGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtc                                                                     <  2:1062031/150‑1 (MQ=255)
                                                                                  cGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtctc                                                                   <  2:488867/150‑1 (MQ=255)
                                                                                    aaCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGc                                                                 <  1:469799/150‑1 (MQ=255)
                                                                                     aCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa                                                                <  1:1587764/150‑1 (MQ=255)
                                                                                      cATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGGGCCCAATGTATGCGTTGCCACGCAGTGAAAATTCCTCTGAAAACGTCTCGCaa                                                               >  2:1378076/1‑150 (MQ=255)
                                                                                        tCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTAGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAg                                                             >  2:220064/1‑150 (MQ=255)
                                                                                        tCCGAATACGCTACTGGCCCAGGAGCTGTCAAAAACTACGTTCTGATGCCCCGAAAGCATCTGGTTACCGGGAATATTTGCGTACACGAATCCGTGCCCAAAGTATTCGTAGAAACGCAGTGATAATTCCTCTGAAAACGTCTCGCATAg                                                             >  2:1407702/1‑150 (MQ=255)
                                                                                          cGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGc                                                           <  1:2152854/150‑1 (MQ=255)
                                                                                             ttACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGa                                                        <  2:1099460/150‑1 (MQ=255)
                                                                                               aCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGaaa                                                      >  1:24588/1‑150 (MQ=255)
                                                                                                cGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTCCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAAc                                                     >  1:19281/1‑150 (MQ=255)
                                                                                                 gCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACt                                                    <  2:527206/150‑1 (MQ=255)
                                                                                                    aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc                                                 >  2:1645107/1‑150 (MQ=255)
                                                                                                    aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc                                                 <  2:1707031/150‑1 (MQ=255)
                                                                                                     cTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCCCGCAAAGGCTGAAACTGGCa                                                >  2:1848805/1‑150 (MQ=255)
                                                                                                         cccAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATg                                            >  2:1105209/1‑150 (MQ=255)
                                                                                                            aGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCa                                         <  2:1942528/150‑1 (MQ=255)
                                                                                                               cgcTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAg                                      >  1:1737790/1‑150 (MQ=255)
                                                                                                                 cTGGCTTAAACTCCTTTCTGCTGCCCGGTCAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGc                                    <  2:1938716/150‑1 (MQ=255)
                                                                                                                   ggCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCt                                  >  2:2191351/1‑150 (MQ=255)
                                                                                                                     cTTAAACTCCTTTCTGATGCGCGGTTAGCATGTGGTTACCGGGTTTTTTTGAGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAATCGTCTCGCAAAGGCTTAAACTGGCAGATGTCAAAGGCCTgg                                <  2:772023/150‑1 (MQ=255)
                                                                                                                     cTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTgg                                <  1:989650/150‑1 (MQ=255)
                                                                                                                           cTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAAc                          >  2:1768242/1‑150 (MQ=255)
                                                                                                                             ccTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCg                        >  1:2179254/1‑150 (MQ=255)
                                                                                                                              cTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGt                       >  2:2136092/1‑150 (MQ=255)
                                                                                                                                caaaaaTGCCCGGTAACCACATGCTTACCGCGCATTTATGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCACTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATACCCGTaa                     >  2:213535/6‑150 (MQ=255)
                                                                                                                                  cTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATg                   >  1:765448/1‑150 (MQ=255)
                                                                                                                                   tGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGt                  <  1:718010/150‑1 (MQ=255)
                                                                                                                                    gATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCATTGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGACAAATCACTGGGATAACCGAAATGTc                 >  1:445116/1‑150 (MQ=255)
                                                                                                                                     aTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcg                >  2:1070140/1‑150 (MQ=255)
                                                                                                                                     aTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcg                <  2:1091034/150‑1 (MQ=255)
                                                                                                                                      tGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgc               >  1:1430397/1‑150 (MQ=255)
                                                                                                                                      agCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgc               <  1:2193383/149‑1 (MQ=255)
                                                                                                                                       gCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGGTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg              <  1:1352019/150‑1 (MQ=255)
                                                                                                                                       gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGTCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg              >  1:951239/1‑150 (MQ=255)
                                                                                                                                         ccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtc            <  2:159779/150‑1 (MQ=255)
                                                                                                                                         ccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtc            >  1:1814712/1‑150 (MQ=255)
                                                                                                                                          cGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtca           <  1:1032501/150‑1 (MQ=255)
                                                                                                                                           ggTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcat          <  1:169412/150‑1 (MQ=255)
                                                                                                                                            gTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatc         <  2:1099712/150‑1 (MQ=255)
                                                                                                                                             tAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatca        <  1:2022406/150‑1 (MQ=255)
                                                                                                                                             tAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTAGCAAAGTCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatca        <  1:119664/150‑1 (MQ=255)
                                                                                                                                               aGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATa      >  1:1643578/1‑150 (MQ=255)
                                                                                                                                                   tgtgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAata  >  2:1421177/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAGAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATA  >  NC_000913/2356590‑2356886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: