Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,356,738 G→A 7.7% intergenic (+27/‑166) glpC → / → yfaD anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,356,7380GA 7.7% 483.1 / 19.2 158intergenic (+27/‑166)glpC/yfaDanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base G (64/80);  new base A (5/7);  total (70/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 163 total aligned reads displayed.

TAGAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATAT  >  NC_000913/2356590‑2356887
                                                                                                                                                    |                                                                                                                                                     
tAGAAGAAATCGGCGCAGATCTTGTGGTCACCGACTGCTAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTATTCGCTGCGAACATCCGATTACGATACTTGGCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCAtgt                                                                                                                                                      <  1:1431108/150‑1 (MQ=255)
  gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                    >  2:569124/1‑150 (MQ=255)
  gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                    <  1:1835391/150‑1 (MQ=255)
  gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                    <  2:1932986/150‑1 (MQ=255)
    agaaAGCGGCGCAGATCTGGTGGTCACCGACTACGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGtt                                                                                                                                                  >  1:1119026/1‑150 (MQ=255)
        aGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCg                                                                                                                                              <  1:853734/150‑1 (MQ=255)
         ccggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCgg                                                                                                                                             <  1:764451/149‑1 (MQ=255)
          gggcgCAGATCTGGTGGTCACCGACTTCGAAACCTGTAAATGGCAGATTGAGATTTAAAAAAGTATTCGCTGCTAACATACGTTTACGCTAATTGCCTATTTGCTGTCTAAAACTGCTTTCTTATGCCCGGTAACCACATGCTTACCggg                                                                                                                                            <  2:1402611/149‑1 (MQ=255)
           ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCCGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGc                                                                                                                                           <  1:1894920/150‑1 (MQ=255)
           ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGc                                                                                                                                           <  2:1103922/150‑1 (MQ=255)
           ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGGCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGc                                                                                                                                           <  1:2131092/150‑1 (MQ=255)
            gcgcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCa                                                                                                                                          >  2:1333885/1‑150 (MQ=255)
              gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                        <  2:1876625/150‑1 (MQ=255)
              gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGATTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                        <  1:1038665/150‑1 (MQ=255)
               cAGATCTGGTGGTCACCGACTGCGAAAGCTGTAAATGGCAGATTGTGATGTCCACAAGTCTTCGCTGCGAACATCAGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAttt                                                                                                                                       <  1:1854036/150‑1 (MQ=255)
                  aTCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGATTAAACTGCTTTCTGATGACCGGTAAGCATGTGGTTACCGGGCATTTTTg                                                                                                                                    <  2:550277/150‑1 (MQ=255)
                   tCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGc                                                                                                                                   >  2:1587764/1‑150 (MQ=255)
                    cTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCCGGCGCTGGCTTAAACTCCTTTCTGCTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                                                                                  <  1:731365/150‑1 (MQ=255)
                    cTGGTGGTCACCGACTGAGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTAGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                                                                                  >  2:2175817/1‑150 (MQ=255)
                      ggtggtCACCGACTGCGAAACCTGTAAATGCCAGATTGCGATGCCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTa                                                                                                                                <  1:213143/150‑1 (MQ=255)
                        tggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTaca                                                                                                                              <  2:1697049/150‑1 (MQ=255)
                           tCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGa                                                                                                                           >  1:814227/1‑150 (MQ=255)
                            cACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGAt                                                                                                                          >  1:740263/1‑150 (MQ=255)
                             aCCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTTCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGAtt                                                                                                                         >  2:1100836/1‑150 (MQ=255)
                             aCCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTTCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGAtt                                                                                                                         >  2:716419/1‑150 (MQ=255)
                              ccGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTc                                                                                                                        >  2:59856/1‑150 (MQ=255)
                               cGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTcc                                                                                                                       >  2:989650/1‑150 (MQ=255)
                               cGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTcc                                                                                                                       >  1:1171138/1‑150 (MQ=255)
                                gACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCg                                                                                                                      >  2:469799/1‑150 (MQ=255)
                                   tGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                                                                   <  2:1501334/150‑1 (MQ=255)
                                   tGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                                                                   <  1:625297/150‑1 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAGACTCCGTTCTGATGCCCGGTAAGCATGTGGTTCCCGGGCTTTATTGCGTACACGATTCCGTGcc                                                                                                                  >  1:321732/1‑150 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGcc                                                                                                                  >  2:2087824/1‑150 (MQ=255)
                                    gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGcc                                                                                                                  <  1:510686/150‑1 (MQ=255)
                                     cGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGccc                                                                                                                 <  2:1923010/150‑1 (MQ=255)
                                          ccTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGt                                                                                                            >  1:159779/1‑150 (MQ=255)
                                            tGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCATTGTAt                                                                                                          >  1:400882/1‑150 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                         >  2:2022406/1‑150 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                         >  2:119664/1‑150 (MQ=255)
                                             gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                         <  2:821028/150‑1 (MQ=255)
                                               aaaTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCg                                                                                                       >  2:1075345/1‑150 (MQ=255)
                                                 aTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGtt                                                                                                     >  1:292632/1‑150 (MQ=255)
                                                   ggCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTATGCTACTGGCCCAGGCGCTTGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGc                                                                                                   <  2:2080410/150‑1 (MQ=255)
                                                   ggCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAGTTTTGCGTACACGATTCCGTGCCCAAAGTATGCGTTGc                                                                                                   >  1:1805844/1‑150 (MQ=255)
                                                    gCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCa                                                                                                  <  2:1959933/150‑1 (MQ=255)
                                                       taTTGAGATGTCCACACGTCTTCGCTGCGAACATCCGATTACGCTACTTGCCGAGGCGCTGGCTTAAACTCCTTTCTTATGCGCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACg                                                                                               <  2:319501/149‑1 (MQ=255)
                                                        aTTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGc                                                                                              <  2:239877/150‑1 (MQ=255)
                                                        aTTGAGATGTCCACAAGGCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGc                                                                                              <  1:1077236/150‑1 (MQ=255)
                                                         ttGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCa                                                                                             <  2:405823/150‑1 (MQ=255)
                                                          tGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAg                                                                                            <  1:806710/150‑1 (MQ=255)
                                                                 tCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTCAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                                                                     <  1:53939/150‑1 (MQ=255)
                                                                    acaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTcc                                                                                  <  1:1333885/150‑1 (MQ=255)
                                                                      aaGTCTTCTCTGCGAACATCCGAGTACGCTACTGGCCCAGGCGCTGGCATAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                                <  1:924932/150‑1 (MQ=255)
                                                                      aaGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                                <  1:1082730/150‑1 (MQ=255)
                                                                      aaGTCATCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                                <  1:933057/150‑1 (MQ=255)
                                                                       aGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCGGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctct                                                                               <  1:234635/150‑1 (MQ=255)
                                                                        gTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTg                                                                              <  2:833114/150‑1 (MQ=255)
                                                                             cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAAc                                                                         <  1:1912448/150‑1 (MQ=255)
                                                                             cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGGGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTGGCAACGCAGTGAAAATTCCTCTGAAAAc                                                                         <  1:1277663/150‑1 (MQ=255)
                                                                              gCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACg                                                                        >  2:1350174/1‑150 (MQ=255)
                                                                                tGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtc                                                                      <  2:1062031/150‑1 (MQ=255)
                                                                                  cGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtctc                                                                    <  2:488867/150‑1 (MQ=255)
                                                                                     aCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa                                                                 <  1:1587764/150‑1 (MQ=255)
                                                                                        tCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTAGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAg                                                              >  2:220064/1‑150 (MQ=255)
                                                                                        tCCGAATACGCTACTGGCCCAGGAGCTGTCAAAAACTACGTTCTGATGCCCCGAAAGCATCTGGTTACCGGGAATATTTGCGTACACGAATCCGTGCCCAAAGTATTCGTAGAAACGCAGTGATAATTCCTCTGAAAACGTCTCGCATAg                                                              >  2:1407702/1‑150 (MQ=255)
                                                                                          cGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGc                                                            <  1:2152854/150‑1 (MQ=255)
                                                                                             ttACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGa                                                         <  2:1099460/150‑1 (MQ=255)
                                                                                              tACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGaa                                                        <  2:1229599/150‑1 (MQ=255)
                                                                                               aCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGaaa                                                       >  1:24588/1‑150 (MQ=255)
                                                                                                cGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTCCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAAc                                                      >  1:19281/1‑150 (MQ=255)
                                                                                                 gCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACt                                                     <  2:527206/150‑1 (MQ=255)
                                                                                                    aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc                                                  <  2:1707031/150‑1 (MQ=255)
                                                                                                     cTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCCCGCAAAGGCTGAAACTGGCa                                                 >  2:1848805/1‑150 (MQ=255)
                                                                                                          ccAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGt                                            >  2:1182622/1‑150 (MQ=255)
                                                                                                            aGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCa                                          <  2:1942528/150‑1 (MQ=255)
                                                                                                                     cTTAAACTCCTTTCTGATGCGCGGTTAGCATGTGGTTACCGGGTTTTTTTGAGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAATCGTCTCGCAAAGGCTTAAACTGGCAGATGTCAAAGGCCTgg                                 <  2:772023/150‑1 (MQ=255)
                                                                                                                      ttAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTggg                                >  2:1771372/1‑150 (MQ=255)
                                                                                                                       tAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGa                               <  1:687851/150‑1 (MQ=255)
                                                                                                                              cTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGt                        >  2:2136092/1‑150 (MQ=255)
                                                                                                                                caaaaaTGCCCGGTAACCACATGCTTACCGCGCATTTATGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCACTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATACCCGTaa                      >  2:213535/6‑150 (MQ=255)
                                                                                                                                   tGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGt                   <  1:718010/150‑1 (MQ=255)
                                                                                                                                    gATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCATTGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGACAAATCACTGGGATAACCGAAATGTc                  >  1:445116/1‑150 (MQ=255)
                                                                                                                                    gATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTc                  >  2:1025791/1‑150 (MQ=255)
                                                                                                                                      tGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgc                >  1:1430397/1‑150 (MQ=255)
                                                                                                                                      agCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgc                <  1:2193383/149‑1 (MQ=255)
                                                                                                                                       gCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGGTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg               <  1:1352019/150‑1 (MQ=255)
                                                                                                                                       gCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGGTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg               <  2:1119026/150‑1 (MQ=255)
                                                                                                                                       gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGTCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg               >  1:951239/1‑150 (MQ=255)
                                                                                                                                       acccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGAGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGATACTGGCAGCTGTCAAAGGCCTGGGATACCCGTAATGTcgcg               >  1:423049/2‑150 (MQ=255)
                                                                                                                                        cccGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGt              >  2:1092423/1‑150 (MQ=255)
                                                                                                                                         ccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtc             <  2:159779/150‑1 (MQ=255)
                                                                                                                                         ccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtc             >  1:1814712/1‑150 (MQ=255)
                                                                                                                                          cGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtca            <  1:1032501/150‑1 (MQ=255)
                                                                                                                                           ggTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcat           <  1:169412/150‑1 (MQ=255)
                                                                                                                                             tAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatca         <  1:2022406/150‑1 (MQ=255)
                                                                                                                                             tAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTAGCAAAGTCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatca         <  1:119664/150‑1 (MQ=255)
                                                                                                                                               aGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATa       >  1:1643578/1‑150 (MQ=255)
                                                                                                                                                gCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATaa      >  2:1680982/1‑150 (MQ=255)
                                                                                                                                                   tgtgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAata   >  1:225956/1‑150 (MQ=255)
                                                                                                                                                   tgtgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTCGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAata   >  1:218732/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TAGAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATAT  >  NC_000913/2356590‑2356887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: