Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,356,741 G→C 7.4% intergenic (+30/‑163) glpC → / → yfaD anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,356,7410GC 7.4% 520.7 / 24.7 160intergenic (+30/‑163)glpC/yfaDanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base G (68/80);  new base C (5/7);  total (73/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 165 total aligned reads displayed.

GAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATATCAG  >  NC_000913/2356592‑2356890
                                                                                                                                                     |                                                                                                                                                     
gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                       >  2:569124/1‑150 (MQ=255)
gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                       <  1:1835391/150‑1 (MQ=255)
gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                                       <  2:1932986/150‑1 (MQ=255)
      aGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCg                                                                                                                                                 >  1:1566565/1‑150 (MQ=255)
       gctgcgcAGATCTGGTGGTCACCTACTGCGAAACCTGTAAATGGCAGATTGTGATGTCCACATGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGATTAAACTCCTTTCTTATGCCCGGTAACCACATGCTTACCgg                                                                                                                                                <  1:868583/147‑1 (MQ=255)
       ccggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCgg                                                                                                                                                <  1:764451/149‑1 (MQ=255)
        cggcgCAGATCTGGTGGTCACCGACTGCGAAACCTCTAACTGCCAGATTGAGATGTCCACAAGTCCTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                                                                               <  2:132520/150‑1 (MQ=255)
         ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGc                                                                                                                                              <  2:1103922/150‑1 (MQ=255)
         ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGGCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGc                                                                                                                                              <  1:2131092/150‑1 (MQ=255)
          gcgcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCa                                                                                                                                             >  2:1333885/1‑150 (MQ=255)
            gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                           <  1:415823/150‑1 (MQ=255)
            gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                           <  2:1876625/150‑1 (MQ=255)
            gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                           >  1:1427791/1‑150 (MQ=255)
            gcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGATTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtt                                                                                                                                           <  1:1038665/150‑1 (MQ=255)
              aGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtttt                                                                                                                                         <  2:1204540/150‑1 (MQ=255)
                aTCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGATTAAACTGCTTTCTGATGACCGGTAAGCATGTGGTTACCGGGCATTTTTg                                                                                                                                       <  2:550277/150‑1 (MQ=255)
                aTCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGACATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTg                                                                                                                                       <  2:2087707/150‑1 (MQ=255)
                  cTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCCGGCGCTGGCTTAAACTCCTTTCTGCTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                                                                                     <  1:731365/150‑1 (MQ=255)
                  cTGGTGGTCACCGACTGAGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTAGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                                                                                     >  2:2175817/1‑150 (MQ=255)
                    ggtggtCACCGACTGCGAAACCTGTAAATGGCAGCTTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTa                                                                                                                                   <  1:220249/150‑1 (MQ=255)
                       ggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTacac                                                                                                                                <  1:242459/150‑1 (MQ=255)
                        gtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACg                                                                                                                               >  1:1644368/1‑150 (MQ=255)
                         tCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGa                                                                                                                              >  1:814227/1‑150 (MQ=255)
                         tCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGAATGTGGTTACCGGGCATTTTTGCGTACACGa                                                                                                                              >  1:1829482/1‑150 (MQ=255)
                           aCCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTTCGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGAtt                                                                                                                            >  2:716419/1‑150 (MQ=255)
                            ccGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTc                                                                                                                           >  2:59856/1‑150 (MQ=255)
                             cGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTcc                                                                                                                          >  2:989650/1‑150 (MQ=255)
                             cGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTcc                                                                                                                          >  1:1171138/1‑150 (MQ=255)
                                cTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTg                                                                                                                       >  1:1229599/1‑150 (MQ=255)
                                 tGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                                                                      <  1:625297/150‑1 (MQ=255)
                                 ggCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTATCTGAGGCCCGTTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGc                                                                                                                      <  1:356824/149‑1 (MQ=255)
                                  gCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACAGGGCATTTTTGCGTACACGATTCCGTGcc                                                                                                                     >  1:1313006/1‑150 (MQ=255)
                                        ccTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGt                                                                                                               >  1:159779/1‑150 (MQ=255)
                                         cTGTAAATGTCAGATTGAGAGGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGACGCCCGGTAAGCATTTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTa                                                                                                              <  1:266534/150‑1 (MQ=255)
                                           gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                            >  2:119664/1‑150 (MQ=255)
                                           gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                            >  2:2022406/1‑150 (MQ=255)
                                           gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                            <  2:821028/150‑1 (MQ=255)
                                               aTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGtt                                                                                                        >  1:292632/1‑150 (MQ=255)
                                                 ggCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTATGCTACTGGCCCAGGCGCTTGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGc                                                                                                      <  2:2080410/150‑1 (MQ=255)
                                                 ggCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAGTTTTGCGTACACGATTCCGTGCCCAAAGTATGCGTTGc                                                                                                      >  1:1805844/1‑150 (MQ=255)
                                                     taTTGAGATGTCCACACGTCTTCGCTGCGAACATCCGATTACGCTACTTGCCGAGGCGCTGGCTTAAACTCCTTTCTTATGCGCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACg                                                                                                  <  2:319501/149‑1 (MQ=255)
                                                      aTTGAGATGTCCACAAGGCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGc                                                                                                 <  1:1077236/150‑1 (MQ=255)
                                                       ttGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCa                                                                                                <  2:405823/150‑1 (MQ=255)
                                                        tGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAg                                                                                               <  1:806710/150‑1 (MQ=255)
                                                         gagaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGt                                                                                              >  2:1948955/1‑150 (MQ=255)
                                                               tCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTCAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                                                                        <  1:53939/150‑1 (MQ=255)
                                                               tCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAt                                                                                        <  1:733301/150‑1 (MQ=255)
                                                                 cacaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTc                                                                                      >  2:169412/1‑150 (MQ=255)
                                                                  acaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTcc                                                                                     <  1:1333885/150‑1 (MQ=255)
                                                                    aaGTCTTCTCTGCGAACATCCGAGTACGCTACTGGCCCAGGCGCTGGCATAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                                   <  1:924932/150‑1 (MQ=255)
                                                                    aaGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc                                                                                   <  1:1082730/150‑1 (MQ=255)
                                                                      gTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTg                                                                                 <  2:833114/150‑1 (MQ=255)
                                                                       tCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGa                                                                                <  2:1566565/150‑1 (MQ=255)
                                                                         ttCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTTGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGaaa                                                                              <  2:1158718/150‑1 (MQ=255)
                                                                           cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAAc                                                                            <  1:1912448/150‑1 (MQ=255)
                                                                                cGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGtctc                                                                       <  2:488867/150‑1 (MQ=255)
                                                                                  aaCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGc                                                                     <  1:469799/150‑1 (MQ=255)
                                                                                   aCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa                                                                    <  1:1141707/150‑1 (MQ=255)
                                                                                   aCATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa                                                                    <  1:1587764/150‑1 (MQ=255)
                                                                                    cATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGGGCCCAATGTATGCGTTGCCACGCAGTGAAAATTCCTCTGAAAACGTCTCGCaa                                                                   >  2:1378076/1‑150 (MQ=255)
                                                                                      tCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTAGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAg                                                                 >  2:220064/1‑150 (MQ=255)
                                                                                      tCCGAATACGCTACTGGCCCAGGAGCTGTCAAAAACTACGTTCTGATGCCCCGAAAGCATCTGGTTACCGGGAATATTTGCGTACACGAATCCGTGCCCAAAGTATTCGTAGAAACGCAGTGATAATTCCTCTGAAAACGTCTCGCATAg                                                                 >  2:1407702/1‑150 (MQ=255)
                                                                                        cGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGc                                                               <  1:2152854/150‑1 (MQ=255)
                                                                                        cGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGc                                                               <  1:1898678/150‑1 (MQ=255)
                                                                                         gATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCt                                                              >  1:186213/1‑150 (MQ=255)
                                                                                               gCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACt                                                        <  2:527206/150‑1 (MQ=255)
                                                                                                  aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc                                                     >  2:1645107/1‑150 (MQ=255)
                                                                                                  aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc                                                     <  2:1707031/150‑1 (MQ=255)
                                                                                                   cTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCCCGCAAAGGCTGAAACTGGCa                                                    >  2:1848805/1‑150 (MQ=255)
                                                                                                     ggCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTTGCAGa                                                  >  1:2133678/1‑150 (MQ=255)
                                                                                                      gCCCAGGCGCTGGCTTAAACTCCTTTCTGCTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGGACACGATTCCGTTCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGAt                                                 <  2:436931/150‑1 (MQ=255)
                                                                                                       cccAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATg                                                >  2:1105209/1‑150 (MQ=255)
                                                                                                          aGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCa                                             <  2:1942528/150‑1 (MQ=255)
                                                                                                                   cTTAAACTCCTTTCTGATGCGCGGTTAGCATGTGGTTACCGGGTTTTTTTGAGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAATCGTCTCGCAAAGGCTTAAACTGGCAGATGTCAAAGGCCTgg                                    <  2:772023/150‑1 (MQ=255)
                                                                                                                   cTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTgg                                    <  1:989650/150‑1 (MQ=255)
                                                                                                                     tAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGa                                  >  1:933283/1‑150 (MQ=255)
                                                                                                                     tAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGa                                  <  1:687851/150‑1 (MQ=255)
                                                                                                                         cTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAAc                              >  2:1768242/1‑150 (MQ=255)
                                                                                                                            cTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGt                           <  1:1948955/150‑1 (MQ=255)
                                                                                                                              caaaaaTGCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCTAAGGCCTGGGATAACCGTaa                         >  2:220678/6‑150 (MQ=255)
                                                                                                                              caaaaaTGCCCGGTAACCACATGCTTACCGCGCATTTATGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCACTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATACCCGTaa                         >  2:213535/6‑150 (MQ=255)
                                                                                                                                 tGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGt                      <  1:718010/150‑1 (MQ=255)
                                                                                                                                  gATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTc                     >  2:1025791/1‑150 (MQ=255)
                                                                                                                                   aTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcg                    <  2:1091034/150‑1 (MQ=255)
                                                                                                                                   aTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcg                    >  2:1070140/1‑150 (MQ=255)
                                                                                                                                     gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg                  >  2:2135575/1‑150 (MQ=255)
                                                                                                                                     acccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGAGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGATACTGGCAGCTGTCAAAGGCCTGGGATACCCGTAATGTcgcg                  >  1:423049/2‑150 (MQ=255)
                                                                                                                                      cccGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGt                 >  2:1092423/1‑150 (MQ=255)
                                                                                                                                       ccGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtc                <  2:159779/150‑1 (MQ=255)
                                                                                                                                        cGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtca               <  1:1032501/150‑1 (MQ=255)
                                                                                                                                         ggTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcat              <  1:169412/150‑1 (MQ=255)
                                                                                                                                          gTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatc             <  2:1099712/150‑1 (MQ=255)
                                                                                                                                           tAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatca            <  1:2022406/150‑1 (MQ=255)
                                                                                                                                                 tgtgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAata      >  2:1421177/1‑150 (MQ=255)
                                                                                                                                                 tgtgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAata      >  1:225956/1‑150 (MQ=255)
                                                                                                                                                 tgtgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTCGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAata      >  1:218732/1‑150 (MQ=255)
                                                                                                                                                  gtgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAatat     >  2:1877247/1‑150 (MQ=255)
                                                                                                                                                   tgGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATATc    <  1:1091975/150‑1 (MQ=255)
                                                                                                                                                    ggTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATATCa   <  1:562970/150‑1 (MQ=255)
                                                                                                                                                     gTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATATCAg  <  1:844836/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATAAATATCAG  >  NC_000913/2356592‑2356890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: