Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 10,619 G→T 6.7% intergenic (‑125/+24) satP ← / ← yaaW succinate‑acetate transporter/UPF0174 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,6190GT 6.7% 366.5 / 12.0 120intergenic (‑125/+24)satP/yaaWsuccinate‑acetate transporter/UPF0174 family protein
Reads supporting (aligned to +/- strand):  ref base G (54/58);  new base T (2/6);  total (56/64)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

Only 100 of 138 total aligned reads displayed.

GATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCC  >  NC_000913/10470‑10768
                                                                                                                                                     |                                                                                                                                                     
gATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatg                                                                                                                                                       >  1:91856/1‑150 (MQ=255)
  ttAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatgaa                                                                                                                                                     <  1:1656047/150‑1 (MQ=255)
    aGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGCGGGgaaaatgaaaa                                                                                                                                                   >  2:1472527/1‑150 (MQ=255)
     gCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACTATCTTTTGAGGGgaaaatgaaaat                                                                                                                                                  >  2:54463/1‑150 (MQ=255)
     gCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatgaaaat                                                                                                                                                  >  1:211582/1‑150 (MQ=255)
         aCTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGAGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATATTc                                                                                                                                              >  1:1061535/1‑150 (MQ=255)
          cTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTcc                                                                                                                                             <  1:454642/150‑1 (MQ=255)
           ttAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTccc                                                                                                                                            <  1:1008012/150‑1 (MQ=255)
            tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCTGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTcccc                                                                                                                                           >  2:1790530/1‑150 (MQ=255)
            tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                           <  1:1791923/150‑1 (MQ=255)
            tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                           <  1:351757/150‑1 (MQ=255)
            tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                           <  2:860415/150‑1 (MQ=255)
            tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                           <  2:782423/150‑1 (MQ=255)
            tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTcccc                                                                                                                                           <  1:689152/150‑1 (MQ=255)
               tgtTTCCCATAATTACTCAAAAATCATCATCGAATGAATGGTGAAATAATTTAACTGAATAACTGTAGTGTTTTCAGGGCGCGGCAAAAAAATCAGCAAGTCGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCACGGt                                                                                                                                        >  2:1338770/1‑150 (MQ=255)
                     ccctAAATCCTCCAAAAACATCATCGAATGAATTCTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAAACAGCCAGTGGGTCAGTGTCTACGATGTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCgg                                                                                                                                  <  2:370205/147‑1 (MQ=255)
                     ccATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCgg                                                                                                                                  >  2:1788674/1‑150 (MQ=255)
                      cATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGt                                                                                                                                 >  2:799281/1‑150 (MQ=255)
                      cATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCACCGGTTTCCGGt                                                                                                                                 >  1:910684/1‑150 (MQ=255)
                        tacttCCTCAAAAATCATCATCGATTGAATCGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGCCATATTAATCAGCCAGTGGGGCTGTGTCTCCGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTAt                                                                                                                               <  1:2079777/147‑1 (MQ=255)
                         aaTTACTCAAAAATCATCATCGAATGAATTGTGAAATAATTTCCCTGAATAACTGAAGTGTTTTCAGGGCGCGGCATATTAATCAGAGAGTGGTGCAGTTTCGACGATCTTTTGAGGGGAATATGAAATTTTTCCCCGTGTTCCGGTAta                                                                                                                              >  2:135991/1‑149 (MQ=255)
                                cAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTg                                                                                                                       <  2:1665574/150‑1 (MQ=255)
                                cAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTg                                                                                                                       >  2:1380293/1‑150 (MQ=255)
                                    aaTCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATTATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTg                                                                                                                   <  2:1848650/150‑1 (MQ=255)
                                     atcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTgg                                                                                                                  <  2:1507541/150‑1 (MQ=255)
                                     atcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTgg                                                                                                                  <  1:1842495/150‑1 (MQ=255)
                                     atcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTgg                                                                                                                  >  1:2200705/1‑150 (MQ=255)
                                     atcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTgg                                                                                                                  <  2:2214504/150‑1 (MQ=255)
                                        atcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGgcgc                                                                                                               <  2:610153/150‑1 (MQ=255)
                                        atcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGgcgc                                                                                                               <  2:855498/150‑1 (MQ=255)
                                         tcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCt                                                                                                              <  1:1825112/150‑1 (MQ=255)
                                          catcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTa                                                                                                             >  1:1142893/1‑150 (MQ=255)
                                          catcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGCTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTa                                                                                                             <  1:1568514/150‑1 (MQ=255)
                                            tcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAAc                                                                                                           <  2:11023/150‑1 (MQ=255)
                                            tcGAATGAATGGTGAAATAATATCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGAACTATTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTAACAGACCTGAGTGGCGCTAAc                                                                                                           >  2:10594/1‑150 (MQ=255)
                                                atgaatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTAGAAGACCTGAGTGGCGCTAACCATc                                                                                                       >  1:1746300/1‑150 (MQ=255)
                                                  taatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGTGGCAGTGTCTTCGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGAGCTGAGTGGCGCTAACCATCCg                                                                                                     <  2:213153/149‑1 (MQ=255)
                                                   aatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCgg                                                                                                    <  1:315127/150‑1 (MQ=255)
                                                    atgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGgc                                                                                                   <  1:812078/150‑1 (MQ=255)
                                                     tgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGgcg                                                                                                  >  2:1978505/1‑150 (MQ=255)
                                                       gTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGTCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCgca                                                                                                >  1:1141500/1‑150 (MQ=255)
                                                       gTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCgca                                                                                                <  1:494063/150‑1 (MQ=255)
                                                               atTTCCCTGAATAACTGTAGTGTTTCCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCg                                                                                        <  2:63954/150‑1 (MQ=255)
                                                                    ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   >  1:2089071/1‑150 (MQ=255)
                                                                    ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   <  2:1457475/150‑1 (MQ=255)
                                                                    ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   <  1:525858/150‑1 (MQ=255)
                                                                    ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCACCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGTCGCAGGCAGGCGAtttt                                                                                   >  1:879592/1‑149 (MQ=255)
                                                                     cTGAATAACTGTAGTGTTTTCAGGGAGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGc                                                                                  >  2:369205/1‑150 (MQ=255)
                                                                     cTGAATAACTGTAGGGTTTGCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGc                                                                                  <  1:314984/150‑1 (MQ=255)
                                                                       gAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCACAGGCAGGCGATTTGCAg                                                                                >  1:900135/1‑150 (MQ=255)
                                                                        aaTAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCTGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                                               <  1:1790530/150‑1 (MQ=255)
                                                                           aaCTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACg                                                                            >  1:281373/1‑150 (MQ=255)
                                                                             cTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGc                                                                          <  2:876400/150‑1 (MQ=255)
                                                                               gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTg                                                                        <  2:1924974/150‑1 (MQ=255)
                                                                               gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGGGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTg                                                                        <  1:843866/150‑1 (MQ=255)
                                                                                 aGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGCGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTACCCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGa                                                                      >  1:1712592/1‑150 (MQ=255)
                                                                                  gtgtTTTCAGGGCGCGGCATCATCATCATCCAGTGGGTCAGGGGCTACGAGCTTTTGAGTGGAAACTGAAAATTTTCCCCGGTTTCAGGTATCAGACCTGAGGTTCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGaa                                                                     <  1:630679/150‑1 (MQ=255)
                                                                                   tgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAAt                                                                    >  1:1227617/1‑150 (MQ=255)
                                                                                    gtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCTGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATc                                                                   >  2:909416/1‑150 (MQ=255)
                                                                                      tttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGt                                                                 >  2:1779740/1‑150 (MQ=255)
                                                                                        tCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCa                                                               >  1:1733759/1‑150 (MQ=255)
                                                                                           gggCGCGGCATAATAATCAGCCAGTGGGGCAGTTTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCAcgc                                                            <  2:2204561/150‑1 (MQ=255)
                                                                                           gggCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCAcgc                                                            <  2:129390/150‑1 (MQ=255)
                                                                                               gcgGCATAATAATCAGCCCGGGCGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATa                                                        <  1:102599/150‑1 (MQ=255)
                                                                                                  gCAAAATAATCAGCCAGTGGGGCAGTGTCTACCATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGCCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgc                                                     <  2:1392403/150‑1 (MQ=255)
                                                                                                   cATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgcg                                                    <  2:299598/150‑1 (MQ=255)
                                                                                                        taatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGcc                                               >  1:1022826/1‑150 (MQ=255)
                                                                                                          atCAGACAGGGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGGGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGc                                             <  2:2014058/150‑1 (MQ=255)
                                                                                                             aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTgc                                          >  1:815799/1‑149 (MQ=255)
                                                                                                              gCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAc                                         <  2:1879357/150‑1 (MQ=255)
                                                                                                               ccAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAcc                                        <  1:56246/150‑1 (MQ=255)
                                                                                                               ccAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAcc                                        <  1:799281/150‑1 (MQ=255)
                                                                                                               ccAGTGCGGCAGGGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAcc                                        <  1:130668/150‑1 (MQ=255)
                                                                                                                    ggggCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCttt                                   <  1:1314163/150‑1 (MQ=255)
                                                                                                                     gggCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTa                                  >  1:2005322/1‑150 (MQ=255)
                                                                                                                      ggCAGTGTCTACGATCTTTTGAGGGGAAAAAGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCCCAGGCAGGCGATTTGCAGTACGGCAGGAATCGTCACGCGATAGGCGATGCCGCTGACCGCTATaa                                 >  2:1590334/1‑150 (MQ=255)
                                                                                                                        cAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAcc                               <  2:910684/150‑1 (MQ=255)
                                                                                                                        cAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAcc                               <  2:1308805/150‑1 (MQ=255)
                                                                                                                             tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAtt                          >  2:1327082/1‑150 (MQ=255)
                                                                                                                              cTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAttt                         >  2:1250948/1‑150 (MQ=255)
                                                                                                                                aCGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAg                       >  2:136727/1‑150 (MQ=255)
                                                                                                                                aCGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAg                       <  2:1142893/150‑1 (MQ=255)
                                                                                                                                  gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                     >  2:555770/1‑150 (MQ=255)
                                                                                                                                  gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                     >  1:924563/1‑150 (MQ=255)
                                                                                                                                  gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGAGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                     >  2:320161/1‑150 (MQ=255)
                                                                                                                                  gATCTTTTGAGGGGAAAATGAAAATTTTCCACGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                     <  2:408375/150‑1 (MQ=255)
                                                                                                                                   aTCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGc                    <  2:223317/150‑1 (MQ=255)
                                                                                                                                     cTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGTGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCg                  >  1:1624767/1‑150 (MQ=255)
                                                                                                                                        ttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCAc               >  1:614132/1‑150 (MQ=255)
                                                                                                                                         tGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCAcc              <  1:1472527/150‑1 (MQ=255)
                                                                                                                                           aGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTa            >  2:2212909/1‑150 (MQ=255)
                                                                                                                                            ggggAAAATTTTCATTTTCCCCGGTTTCAGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCCCCTAc           >  2:1425074/1‑150 (MQ=255)
                                                                                                                                            ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTAc           <  2:2089071/150‑1 (MQ=255)
                                                                                                                                            ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGATGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTAc           >  2:1005968/1‑150 (MQ=255)
                                                                                                                                              ggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAg         >  1:1654896/1‑150 (MQ=255)
                                                                                                                                               gtaaatgaaaatTTTCCCCGGTTTCCGGTATCACTCCGGCGTGGGGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAgg        <  2:638429/148‑1 (MQ=255)
                                                                                                                                                 aaatgaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAGCCCATTTAGTGCCGCACCTACAggtc      >  2:1634187/1‑148 (MQ=255)
                                                                                                                                                  aatgaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGcc     <  1:1965539/150‑1 (MQ=255)
                                                                                                                                                    tgaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTc   >  1:1987937/1‑150 (MQ=255)
                                                                                                                                                     gaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTcc  >  2:1453873/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCC  >  NC_000913/10470‑10768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑