Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,414,689 C→G 5.3% intergenic (+17/‑58) ackA → / → pta acetate kinase A and propionate kinase 2/phosphate acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,414,6890CG 5.3% 415.7 / 12.6 132intergenic (+17/‑58)ackA/ptaacetate kinase A and propionate kinase 2/phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (75/50);  new base G (5/2);  total (80/52)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-01

Only 100 of 140 total aligned reads displayed.

CATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGG  >  NC_000913/2414541‑2414838
                                                                                                                                                    |                                                                                                                                                     
cATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTCGTTATCGCGCAAGACGCGACCCGCCTGACTGCCTGATTTCACACCGCcagctca                                                                                                                                                      >  1:249748/1‑150 (MQ=255)
 aTGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCctgctcag                                                                                                                                                     <  1:274549/150‑1 (MQ=255)
  tGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctcagc                                                                                                                                                    >  1:272337/1‑150 (MQ=255)
   gAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCATCAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctcagct                                                                                                                                                   >  2:898619/1‑150 (MQ=255)
   gAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctcagct                                                                                                                                                   >  1:1731223/1‑150 (MQ=255)
   caaCGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGGCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctcagct                                                                                                                                                   <  1:2079945/149‑1 (MQ=255)
    aaCGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTg                                                                                                                                                  >  2:156808/1‑150 (MQ=255)
        cacccTGGCTGCACGTTTCGGCAAAGCTGGTTTAATCAACAAAGAAGGGACCCGGCCTGCGGTGGTTATACCAAACAAAGAAGAACTGGTTATCGCGAAAGACGCGAGACGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTTGCgg                                                                                                                                              <  1:794002/147‑1 (MQ=255)
        cAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCgg                                                                                                                                              <  1:185504/150‑1 (MQ=255)
         aaCCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGt                                                                                                                                             <  1:1576220/150‑1 (MQ=255)
          aCCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAACGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTGAGCTGGCGGTg                                                                                                                                            <  1:390808/150‑1 (MQ=255)
           ccTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGc                                                                                                                                           <  2:1423753/150‑1 (MQ=255)
                cTGCACGTTTCGGCAAATCTGGTTTCATCATCAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGttt                                                                                                                                      <  1:914162/150‑1 (MQ=255)
                cTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGttt                                                                                                                                      >  2:159039/1‑150 (MQ=255)
                 tGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGtttt                                                                                                                                     >  2:1837658/1‑150 (MQ=255)
                  gaaCGTTTGGGCAAATCTGGTTTCATCAACAAAGATCGAAGCCGTCCTGCGGTGGTTATCACAACGAAAGAATAACTGGTTATCGCGCAAGACGCGAGCCTCCTGACTGCCTGTTTTCACACCGCGAGATCTGCTGGCGGTGCTGTTTTg                                                                                                                                    <  2:931173/148‑1 (MQ=255)
                  gCACGTTTCGTCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCACAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCAGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTg                                                                                                                                    <  2:2042211/150‑1 (MQ=255)
                     cGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTaa                                                                                                                                 >  2:750008/1‑150 (MQ=255)
                        ttCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAAccc                                                                                                                              <  1:679200/150‑1 (MQ=255)
                           ggCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGcc                                                                                                                           >  2:1646410/1‑150 (MQ=255)
                           ggCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGcc                                                                                                                           >  1:1861425/1‑150 (MQ=255)
                           ggCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGcc                                                                                                                           >  1:1158549/1‑150 (MQ=255)
                            gCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGCGCCGCCTGCCTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCa                                                                                                                          >  2:262141/1‑150 (MQ=255)
                                   tGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcggc                                                                                                                   <  2:1455303/150‑1 (MQ=255)
                                   tGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcggc                                                                                                                   <  1:2151244/150‑1 (MQ=255)
                                      tttCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGt                                                                                                                >  1:398592/1‑150 (MQ=255)
                                         catcaACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCGATTtggcgggtta                                                                                                             <  2:480163/150‑5 (MQ=255)
                                         catcaACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAAc                                                                                                             >  1:247286/1‑150 (MQ=255)
                                          atcaACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACg                                                                                                            >  2:2086525/1‑150 (MQ=255)
                                          atcaACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACg                                                                                                            >  1:84190/1‑150 (MQ=255)
                                           tcaACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCATCGAATAACTGGTTATCGCGCAAGACGCTAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAATTCGGCGGTAACGa                                                                                                           <  1:1535805/150‑1 (MQ=255)
                                            caacaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAGGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                                                          >  2:649509/1‑150 (MQ=255)
                                            caacaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                                                          >  1:535035/1‑150 (MQ=255)
                                            aaacaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACACACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCAGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                                                          >  1:1309284/2‑150 (MQ=255)
                                             aacaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGGGGTGCTGGTTTGTAACCCGCCAAATCGGCGGTAACGaaa                                                                                                         >  1:1377162/1‑150 (MQ=255)
                                             aacaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaaa                                                                                                         >  1:1885545/1‑150 (MQ=255)
                                              acaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                                                        >  1:1942458/1‑150 (MQ=255)
                                              acaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                                                        >  2:1757781/1‑150 (MQ=255)
                                               caaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAaga                                                                                                       >  1:1625927/1‑150 (MQ=255)
                                                 aagaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAgg                                                                                                     <  2:787692/150‑1 (MQ=255)
                                                 aagaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAgg                                                                                                     <  2:2151103/150‑1 (MQ=255)
                                                  agaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGa                                                                                                    <  2:1632417/150‑1 (MQ=255)
                                                  agaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCGGTTTTGTAACCCGCCAAATAGGCGGTAACGAAAGAGGa                                                                                                    >  2:1127114/1‑150 (MQ=255)
                                                    aagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATa                                                                                                  >  2:1294946/1‑150 (MQ=255)
                                                      ggTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACAGACTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTATAACCCGCCAAATCGGCGTTAACGAAAGAGGATaaa                                                                                                >  2:1130310/1‑150 (MQ=255)
                                                        tACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAAcc                                                                                              >  1:256921/1‑150 (MQ=255)
                                                         aCCCGGCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACGGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCg                                                                                             <  2:1407325/150‑1 (MQ=255)
                                                          cccGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCgt                                                                                            >  1:1094213/1‑150 (MQ=255)
                                                             gTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                                                         <  1:1059959/150‑1 (MQ=255)
                                                                cTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGAATTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                                      <  1:1323363/150‑1 (MQ=255)
                                                                  gCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGta                                                                                    >  2:1447193/1‑150 (MQ=255)
                                                                         ttATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattattat                                                                             >  2:1062653/1‑150 (MQ=255)
                                                                         ttATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattattat                                                                             >  2:1069795/1‑150 (MQ=255)
                                                                           aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGc                                                                           <  2:236/150‑1 (MQ=255)
                                                                            tCCCTACCAACGAAGAACTGGTTATCGCGCATGACGCGTGCCGCCTGACTGCCTGATTTCACACCTCCAGCTCAGCTGGCGGTGCTGTTTTGTATCCCGCCAAATCGGCTGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCt                                                                          <  2:1309284/150‑1 (MQ=255)
                                                                            tCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTATTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCt                                                                          >  2:2078927/1‑150 (MQ=255)
                                                                             cccAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTg                                                                         >  1:591342/1‑150 (MQ=255)
                                                                             cccAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTg                                                                         <  1:1069795/150‑1 (MQ=255)
                                                                              ccaaccaaCGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGa                                                                        >  2:970307/1‑150 (MQ=255)
                                                                                 accaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATcc                                                                     <  1:865603/150‑1 (MQ=255)
                                                                                  ccaacGAAGAACTGGTTATCGCGCAAGACGCGCGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTATTGTAACCCGCCTAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTAAGCTGATccc                                                                    >  1:132917/1‑150 (MQ=255)
                                                                                   caacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCt                                                                   >  2:1974923/1‑150 (MQ=255)
                                                                                            aCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAAcc                                                          <  2:2185727/150‑1 (MQ=255)
                                                                                                gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCg                                                      <  2:1601771/150‑1 (MQ=255)
                                                                                                   aTCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAGCCGTGTCCCGTATTATTATGCTGATCCCTACCGGGACCCGCGTCg                                                   >  1:1439196/1‑150 (MQ=255)
                                                                                                   aTCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCg                                                   <  2:1158549/150‑1 (MQ=255)
                                                                                                   aTCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGGCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCg                                                   >  2:128288/1‑150 (MQ=255)
                                                                                                    tCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTGTTGTAACCCGCCAAACCGGCCGTAACGAAAGAGGATAAACCGTGGCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCgg                                                  >  2:392612/1‑150 (MQ=255)
                                                                                                     cgcgCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGt                                                 >  1:1975502/1‑150 (MQ=255)
                                                                                                      gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTc                                                <  1:2055511/150‑1 (MQ=255)
                                                                                                      gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTc                                                <  2:1933161/150‑1 (MQ=255)
                                                                                                       cgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCt                                               >  1:1051442/1‑150 (MQ=255)
                                                                                                        gcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTg                                              >  2:2157748/1‑150 (MQ=255)
                                                                                                         cAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGa                                             <  1:160026/150‑1 (MQ=255)
                                                                                                         cAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGa                                             >  1:45717/1‑150 (MQ=255)
                                                                                                              cgcgAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATc                                                                                                                      >  1:2158566/1‑72 (MQ=255)
                                                                                                              cgcgAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATACCTACCGGAACCAGCGTCGGTCTGACCAGc                                        >  2:959574/1‑150 (MQ=255)
                                                                                                                cgAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCgg                                      >  1:1057041/1‑149 (MQ=255)
                                                                                                                 gAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTc                                     <  2:272337/150‑1 (MQ=255)
                                                                                                                  aGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGGGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCa                                    >  1:1209291/1‑150 (MQ=255)
                                                                                                                   gccgccTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCATACCGGAACCAGCGTCGGTCTGACCAGCGTCAg                                   >  2:655955/1‑150 (MQ=255)
                                                                                                                     ctccTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCAAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc                                 <  2:2166836/148‑1 (MQ=255)
                                                                                                                         tGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGGCAAATCGTCGGTAAAGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTgg                             <  2:1475874/150‑1 (MQ=255)
                                                                                                                         tGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGCCCAGCGTCAGCCTTgg                             >  1:182058/1‑150 (MQ=255)
                                                                                                                            cTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGt                          >  1:816814/1‑150 (MQ=255)
                                                                                                                            cTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGt                          >  2:2001196/1‑150 (MQ=255)
                                                                                                                              gCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGa                        >  2:575477/1‑150 (MQ=255)
                                                                                                                                cTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATc                      >  2:1117147/1‑150 (MQ=255)
                                                                                                                                     ttCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGc                 <  2:1731223/150‑1 (MQ=255)
                                                                                                                                      tCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCa                >  2:544386/1‑150 (MQ=255)
                                                                                                                                       cacaCCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCaa               >  2:1699352/1‑150 (MQ=255)
                                                                                                                                       agcacCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCaa               >  1:2127178/3‑150 (MQ=255)
                                                                                                                                         cacCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATg             >  2:12204/1‑150 (MQ=255)
                                                                                                                                         cacCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATg             <  1:159039/150‑1 (MQ=255)
                                                                                                                                          acCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATgg            >  1:676580/1‑150 (MQ=255)
                                                                                                                                          acCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATgg            >  2:2198632/1‑150 (MQ=255)
                                                                                                                                          acCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATgg            <  1:1145480/150‑1 (MQ=255)
                                                                                                                                            cGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGaa          <  2:398592/150‑1 (MQ=255)
                                                                                                                                             gCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACAAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAAc         >  1:709627/1‑150 (MQ=255)
                                                                                                                                                  ctcagctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATATACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACAAGCGTCAGCCTTGGCGTGATCCGTGCAATTGAACGCaaa    >  2:871504/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGG  >  NC_000913/2414541‑2414838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑