Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,438,874 A→T 5.0% M309L (ATG→TTG)  flk → putative flagella assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,8740AT 5.0% 421.0 / 9.2 140M309L (ATG→TTG) flkputative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base A (63/69);  new base T (3/4);  total (66/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

Only 100 of 145 total aligned reads displayed.

ATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAGTAA  >  NC_000913/2438725‑2439023
                                                                                                                                                     |                                                                                                                                                     
atcgTCTTACTGCGCAATCAACGCTTACTTCTGCCCAGGCCGAGGATATTCGTAATCAGCATTATCAAGGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTAGCataata                                                                                                                                                       >  2:560357/4‑150 (MQ=255)
 tGGTCTTTCTGCGCATTCATCGCTTTCTTCTGCCCAGGCCGAGGATTTTCTTAATCAGGTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAatat                                                                                                                                                      <  1:1271662/150‑1 (MQ=255)
 tGGTCTTTCTGCGCAATCATAGTATTCTTCATCCCAGGCCTAGGATATTGATAATCAGCTTTATCAACTGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTTCTTATTCCTTTTCCACCTATGATCGACACGTTGCAAAatat                                                                                                                                                      <  2:872779/150‑1 (MQ=255)
  gggcTTTCTGCGCAATCCTCGCGTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCTATGATGGACACGTTGCAAAATATg                                                                                                                                                     <  2:235159/147‑1 (MQ=255)
         cTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCCCCGATGATGGACACGTTGCAAAATATGGCAAcgc                                                                                                                                              >  2:2186932/1‑150 (MQ=255)
           gcgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGt                                                                                                                                            >  2:1376729/1‑150 (MQ=255)
            cgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCCATATTTTGCAACGTGTc                                                                                                                                           <  2:92010/150‑1 (MQ=255)
              cAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcca                                                                                                                                         <  2:818847/150‑2 (MQ=255)
              cAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcca                                                                                                                                         <  2:31796/150‑2 (MQ=255)
              cAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTccc                                                                                                                                         >  1:185881/1‑150 (MQ=255)
               aaTCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTccat                                                                                                                                        <  1:1539913/150‑3 (MQ=255)
               aaTCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCcg                                                                                                                                        <  2:2175381/150‑1 (MQ=255)
                      gCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCtgtg                                                                                                                                 >  1:1049524/1‑150 (MQ=255)
                      gCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCtgtg                                                                                                                                 <  2:1292119/150‑1 (MQ=255)
                       cTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGCGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTgg                                                                                                                                <  1:941303/150‑1 (MQ=255)
                         ttcttcTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGAt                                                                                                                              <  1:704945/150‑1 (MQ=255)
                           cttctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTCCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATAc                                                                                                                            >  2:265066/1‑150 (MQ=255)
                             tctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCACCGGCGGGTTAAAGGGATGGAGCCGCGGGTTATGCAACCGCTGCTTACTCCTTTGCCACCGCGGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTg                                                                                                                          <  1:1404237/150‑1 (MQ=255)
                             tctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTg                                                                                                                          >  2:2127266/1‑150 (MQ=255)
                               ttcccAGGCAGATGATATTCTTAATCAGCTTTATCAACGGCGGGGTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                        <  2:1559707/148‑1 (MQ=255)
                               tGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                        <  2:2193291/150‑1 (MQ=255)
                               tGCCCAGGACGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                        <  2:1756876/150‑1 (MQ=255)
                                gCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTa                                                                                                                       >  2:204696/1‑150 (MQ=255)
                                  ccAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGt                                                                                                                     >  2:740474/1‑150 (MQ=255)
                                   cAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTc                                                                                                                    >  1:183231/1‑150 (MQ=255)
                                     ggCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgc                                                                                                                  <  1:1590443/150‑1 (MQ=255)
                                     ggCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgc                                                                                                                  <  2:1919017/150‑1 (MQ=255)
                                        cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGTTCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTGCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGTATACTGTTAGTCGCGAt                                                                                                               <  2:1115430/150‑1 (MQ=255)
                                        cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                               <  1:119840/150‑1 (MQ=255)
                                                ttCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGa                                                                                                       >  1:486450/1‑150 (MQ=255)
                                                  cTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCAACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATAatgatg                                                                                                     >  2:1883644/1‑150 (MQ=255)
                                                    tAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCt                                                                                                   <  1:222807/150‑1 (MQ=255)
                                                       tCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCTGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGt                                                                                                >  2:1886487/1‑150 (MQ=255)
                                                        cAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTc                                                                                               <  1:239723/150‑1 (MQ=255)
                                                          gCTTTATCAACGGCGGGGTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctg                                                                                             <  2:1233959/150‑1 (MQ=255)
                                                           cTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCTTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctcg                                                                                            >  1:407753/1‑148 (MQ=255)
                                                             ttATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggct                                                                                          <  1:1002174/150‑1 (MQ=255)
                                                             ttATCAACGGCGGGTTAAAGGGATTGATACGCGTGTTATGCAACCGCTGCTTCATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACTAGTCCCTCGGTGTGGATAATGTTAGTCTCGATTATCCTGATGCTGGTctggct                                                                                          <  1:1886683/150‑1 (MQ=255)
                                                               aTCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggctgg                                                                                        >  2:205261/1‑150 (MQ=255)
                                                                tCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGt                                                                                       >  2:69860/1‑150 (MQ=255)
                                                                tCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGt                                                                                       <  2:12622/150‑1 (MQ=255)
                                                                  aaCGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTc                                                                                     >  1:859976/1‑150 (MQ=255)
                                                                   aCGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCg                                                                                    <  1:1994107/150‑1 (MQ=255)
                                                                      gcggTTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATCGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGAGATTATCCTGATGCTGGTCTGGCTGGTTCGTTa                                                                                 <  1:353574/150‑1 (MQ=255)
                                                                      gcggGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTa                                                                                 <  2:820117/150‑1 (MQ=255)
                                                                        gggTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGATCTGGCTGGTTCGTTAAc                                                                               <  1:1071300/150‑1 (MQ=255)
                                                                         ggTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcc                                                                              >  1:511643/1‑150 (MQ=255)
                                                                         ggTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcc                                                                              >  2:53500/1‑150 (MQ=255)
                                                                         ggTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCATGATGCTGGTCTGGCTGGTTCGTTAAcc                                                                              >  1:1950542/1‑150 (MQ=255)
                                                                          gTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAccc                                                                             >  2:145243/1‑150 (MQ=255)
                                                                            tAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcg                                                                           <  2:223935/150‑1 (MQ=255)
                                                                                   aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTATTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                    <  1:654640/150‑1 (MQ=255)
                                                                                      gATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGCTGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                                 >  2:3545/1‑150 (MQ=255)
                                                                                      gATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGCCGCAATGAc                                                                 >  2:1455906/1‑150 (MQ=255)
                                                                                      gATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                                 >  1:1778398/1‑150 (MQ=255)
                                                                                      gATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                                 >  1:954104/1‑150 (MQ=255)
                                                                                        gccGCGGGTTATGCAACCGCTGCTTAATCCTGTTCCACCGATGACTGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGACGCTGGTCTGGCTGGGTCGTTAACCCCGACGAAATGACAg                                                               <  2:417787/149‑1 (MQ=255)
                                                                                         ccGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGt                                                              <  1:326/150‑1 (MQ=255)
                                                                                          cgcgTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCTACGCGTCCCGCGCTGTGGATACTGTTTGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTa                                                             <  1:1851209/150‑1 (MQ=255)
                                                                                           gcgTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCATGATGCTGGTCTGGCTGGATCGTTAACACCGACTAAATGACAGTaa                                                            >  2:787602/1‑149 (MQ=255)
                                                                                             gtgtTATGCAACCGCTGCTTAATCCTTTTACACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCg                                                          <  2:209765/150‑1 (MQ=255)
                                                                                                ttATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAACCCGACGAAATGACAGTATCGTGa                                                       >  1:34512/1‑150 (MQ=255)
                                                                                                 tATGCAACCGCTGCTTACTCCTTTTCCACCGATGATGGACTCGTTGCTATATATGGCAACGCGTCCCGCTCTTTGTATACTGTTAGTCGCGATTATCCTGATGCTGTTCTGGCGGGTTCGTTAACCCCGACGAAATGACAGTATCGTGAc                                                      <  2:221240/150‑1 (MQ=255)
                                                                                                 tATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGAc                                                      <  2:1724509/150‑1 (MQ=255)
                                                                                                  aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCTTGACa                                                     >  1:1201659/1‑150 (MQ=255)
                                                                                                  aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACa                                                     >  2:1984307/1‑150 (MQ=255)
                                                                                                   tGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCACGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCTACGAAATGCCAGTATCGTCACaa                                                    >  1:1945686/1‑150 (MQ=255)
                                                                                                    gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                   <  1:615105/150‑1 (MQ=255)
                                                                                                     cAACCGCTGCTTAATCCTTTTCCACCGATGCTGGACACGTTGCAATATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaata                                                  >  2:1172444/1‑150 (MQ=255)
                                                                                                     aaaCCGCTGCTTAATACTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaata                                                  <  2:1377269/149‑1 (MQ=255)
                                                                                                       aCCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataat                                                <  2:183231/150‑1 (MQ=255)
                                                                                                         cGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGCCGAAATGACAGTATCGTGACAATAATAc                                              >  1:1125090/1‑150 (MQ=255)
                                                                                                         cGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAc                                              <  2:1965653/150‑1 (MQ=255)
                                                                                                         cGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAc                                              <  1:1860004/150‑1 (MQ=255)
                                                                                                           ctgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAccc                                            <  1:590684/150‑1 (MQ=255)
                                                                                                            tgctTAATCCTTTTCCACGGTTGATGGACACGTTGCAAAATATGGCATCGCGTCCCTCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCa                                           <  1:1350915/150‑1 (MQ=255)
                                                                                                            tgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCa                                           <  2:2120314/150‑1 (MQ=255)
                                                                                                             gctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAAAATGGCAACGCGTCCCGCGCTGTGGATACTGATAGTCGCGATTATCCTGATGCTGGTCACGCAGGATCGTAAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAg                                          >  1:127849/1‑150 (MQ=255)
                                                                                                                  aTCCTTTTCCACCGATGATGGCCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCAccc                                     >  1:1640625/1‑149 (MQ=255)
                                                                                                                    ccTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCGTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCa                                   <  2:2154660/150‑1 (MQ=255)
                                                                                                                    ccTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCa                                   >  2:2157781/1‑150 (MQ=255)
                                                                                                                     cTTTTCCACCGCTGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCag                                  <  2:565342/150‑1 (MQ=255)
                                                                                                                     cTTTTCCACCGATGATTGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGGTCGTTACCCCCGACGAAATGCCAGTATCGTGACATTAATACCCCGCACCGCag                                  >  1:1848899/1‑150 (MQ=255)
                                                                                                                          ccACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAGCCATCACCGCAGAGATc                             <  2:319683/150‑1 (MQ=255)
                                                                                                                           cacaGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGAGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCg                            <  1:1376729/150‑1 (MQ=255)
                                                                                                                              cGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGccc                         <  1:1129318/150‑1 (MQ=255)
                                                                                                                               gatgatGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGcccc                        >  1:1855530/1‑150 (MQ=255)
                                                                                                                                atgttgGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCg                       <  1:787602/150‑1 (MQ=255)
                                                                                                                                    gggACGCGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCaa                   >  1:796579/2‑150 (MQ=255)
                                                                                                                                      gACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGa                 <  1:452768/150‑1 (MQ=255)
                                                                                                                                      gACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGa                 >  1:2129948/1‑150 (MQ=255)
                                                                                                                                       acacGTTGCAAAATATGGCAACGCGTCCCGCTCTGTGGCTACTGTGAGTCGCGATTATCCTGCTGCTGGTCGGGCTGGTTCGTTAGCCCCGACGAAATGCCAGAATCGTGACAATTATTACCAGCACCGAAGAGATCGCCCCGGCAAGaa                >  1:337773/1‑150 (MQ=255)
                                                                                                                                       acacGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGaa                <  2:746881/150‑1 (MQ=255)
                                                                                                                                        cacGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGaaa               >  2:1541096/1‑150 (MQ=255)
                                                                                                                                          cGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATa             >  1:2003792/1‑150 (MQ=255)
                                                                                                                                            ttGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATAcc           <  2:342974/150‑1 (MQ=255)
                                                                                                                                             tGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCg          >  2:479614/1‑150 (MQ=255)
                                                                                                                                              gCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGa         >  2:1895893/1‑150 (MQ=255)
                                                                                                                                               cAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGaa        >  1:1900909/1‑150 (MQ=255)
                                                                                                                                                aaaaTATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAag       <  1:1740383/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAGTAA  >  NC_000913/2438725‑2439023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: