Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,482,839 C→G 6.4% L167F (TTG→TTC emrK ← multidrug resistance efflux pump membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,482,8390CG 6.4% 544.0 / 21.8 168L167F (TTG→TTCemrKmultidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (88/68);  new base G (5/6);  total (94/74)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

Only 100 of 171 total aligned reads displayed.

CCGGTAACCGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTG  >  NC_000913/2482690‑2482986
                                                                                                                                                     |                                                                                                                                                   
ccGGTAACCTGACTCTTAATATCCGTACGCTTAAGCGCCAACCAGGCTTCTTAAGTTGCATCCGCCGCTTCAACGACTGGGGGCTGACGGTTTAATGGGGTGTTCATAACTATAGCTTTATTCGCTTTATAAGGCTGGATAGCGGCATTc                                                                                                                                                     <  1:317924/150‑1 (MQ=255)
ccGGTAACCGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCGGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTc                                                                                                                                                     >  2:734697/1‑150 (MQ=255)
 cGGTAACCGGAATCTTAATATCCGTACGTTTAATCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACTGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCa                                                                                                                                                    <  2:451846/150‑1 (MQ=255)
  ggTAACCGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCaa                                                                                                                                                   >  1:1877415/1‑150 (MQ=255)
  ggTAACCGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCaa                                                                                                                                                   <  2:531186/150‑1 (MQ=255)
   gTAACCGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAAt                                                                                                                                                  <  2:1239274/150‑1 (MQ=255)
   gTAACCGGACTCATAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACTGTTTAATGGTGTGTTCATTAATTAAGCTTTATTCGCTTTATATGCCTGGATAGCGGCATTCAAt                                                                                                                                                  >  2:113044/1‑150 (MQ=255)
    tAACCGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATg                                                                                                                                                 >  1:2094330/1‑150 (MQ=255)
      aCCGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGcc                                                                                                                                               <  2:1127190/150‑1 (MQ=255)
       ccGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCg                                                                                                                                              >  1:1009226/1‑150 (MQ=255)
       ccGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCg                                                                                                                                              >  2:1978876/1‑150 (MQ=255)
        cGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGc                                                                                                                                             <  1:498036/150‑1 (MQ=255)
         ggACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCt                                                                                                                                            >  2:1585030/1‑150 (MQ=255)
          gACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTGAATGCCGCta                                                                                                                                           <  1:1301456/150‑2 (MQ=255)
           aCTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTGAATGCCGCtat                                                                                                                                          <  2:1071635/150‑3 (MQ=255)
           aCTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCttt                                                                                                                                          <  1:995576/150‑1 (MQ=255)
           aCTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATAACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTGAATGCCGCtat                                                                                                                                          <  1:1487873/150‑3 (MQ=255)
            ctctTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAATCCTGGATAGCGGCATTCAATGCCGCTTTg                                                                                                                                         >  1:1339825/1‑150 (MQ=255)
              ctTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTGAATGCCGCtatcca                                                                                                                                       <  1:2042076/150‑6 (MQ=255)
              ctTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCt                                                                                                                                       >  2:84910/1‑150 (MQ=255)
                tAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTAc                                                                                                                                     <  1:1522531/150‑1 (MQ=255)
                 aaTATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTTAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACt                                                                                                                                    <  2:1393818/150‑1 (MQ=255)
                  atatCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACtt                                                                                                                                   <  1:1086764/150‑1 (MQ=255)
                   tatCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTAATCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACtta                                                                                                                                  <  1:623926/150‑1 (MQ=255)
                        gTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAAc                                                                                                                             >  2:1909064/1‑150 (MQ=255)
                         tACGTTTAAGCGCCAACCAGGCTTCTATAGTTGCATCCGCCGCTTCAACGACATGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGATTTATAAGCCTGGATAGAGGCATTCAAAGCCGCTTTCCTACTTATTAACg                                                                                                                            >  1:1121399/1‑150 (MQ=255)
                           cGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTa                                                                                                                          <  2:1753011/150‑1 (MQ=255)
                           cGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCAGTCAATGCCGCTTTGCTACTTATTTACGTa                                                                                                                          >  1:1428105/1‑150 (MQ=255)
                            gTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTAt                                                                                                                         >  1:1626249/1‑150 (MQ=255)
                                 agctcgaaCCAGGCTTCTTTAGTTGCTTCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTg                                                                                                                    <  1:369528/144‑1 (MQ=255)
                                 aGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTg                                                                                                                    >  1:1239307/1‑150 (MQ=255)
                                  gcgcCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGAGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTgg                                                                                                                   >  1:2146924/1‑150 (MQ=255)
                                     ccAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTAt                                                                                                                <  2:1285982/150‑1 (MQ=255)
                                       aaCCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGc                                                                                                              <  2:739245/150‑1 (MQ=255)
                                       aaCCAGGCTCCAATAGTTGCATCCGCCGCTTCAACGACTAGTGGCTGACGTTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGAAAGCGGCATTCAATGCCGCTTTGCTACTTATAAACGTATCTTTGGTATGc                                                                                                              <  2:184613/150‑1 (MQ=255)
                                           aGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCTTTCTATGCCGCTTTGCTACTTATTAACGTATCGTTGGTATGCTCCa                                                                                                          >  1:432329/1‑150 (MQ=255)
                                                tcttTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGACGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGtt                                                                                                     >  1:191216/1‑150 (MQ=255)
                                                 cttTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATACGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGttt                                                                                                    >  2:1566035/1‑150 (MQ=255)
                                                    tAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCtt                                                                                                 >  1:906761/1‑150 (MQ=255)
                                                         gCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaa                                                                                            <  1:2000080/150‑1 (MQ=255)
                                                          cATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaaa                                                                                           <  2:246568/150‑1 (MQ=255)
                                                            tCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATa                                                                                         <  2:801737/150‑1 (MQ=255)
                                                             ccgccgCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATaa                                                                                        >  1:981538/1‑150 (MQ=255)
                                                              cgccgcTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTAATAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAAc                                                                                       >  1:1050237/1‑150 (MQ=255)
                                                                  cctccAACCACTGCTGGCTGACGCTAAAATGGTGCGTTCATTACTAAACCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCATTGGTATGCTCCAGCGTTTCTTTTGAAATAAccccc                                                                                   <  2:899917/146‑1 (MQ=255)
                                                                   cTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCt                                                                                  >  2:636714/1‑150 (MQ=255)
                                                                      cAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCt                                                                               >  2:804022/1‑150 (MQ=255)
                                                                          gACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGc                                                                           <  1:1724080/150‑1 (MQ=255)
                                                                          gACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGc                                                                           <  1:151976/150‑1 (MQ=255)
                                                                              tgtgGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAAc                                                                       >  2:1199700/1‑150 (MQ=255)
                                                                               gtgGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACg                                                                      >  2:498791/1‑150 (MQ=255)
                                                                                     gACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGTACTc                                                                >  2:1098002/1‑150 (MQ=255)
                                                                                     gACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTc                                                                >  1:583651/1‑150 (MQ=255)
                                                                                     gACGGTTTAATGGTGTGTTCATTACTAAAGCCTAATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTACCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTc                                                                <  2:1047318/150‑1 (MQ=255)
                                                                                      aCGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCg                                                               <  2:2023674/150‑1 (MQ=255)
                                                                                       cGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGa                                                              >  1:1824354/1‑150 (MQ=255)
                                                                                       cGGTTTAATCGTGTGATCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCACCTGCTTCGCTAACGGCACTCGa                                                              >  2:298512/1‑150 (MQ=255)
                                                                                             aaTGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTta                                                        <  1:53537/150‑1 (MQ=255)
                                                                                               tGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTtata                                                      <  2:2150627/150‑1 (MQ=255)
                                                                                                  tgtgtTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTATCGGCACTCGACGGTTATAAta                                                   >  2:1109730/1‑149 (MQ=255)
                                                                                                  tgtgtTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAAtc                                                   >  2:566476/1‑150 (MQ=255)
                                                                                                  tgtgtTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAAtc                                                   >  2:558452/1‑150 (MQ=255)
                                                                                                     gtTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAAtcttc                                                <  1:1214430/150‑1 (MQ=255)
                                                                                                      ttCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAAtcttct                                               <  1:282885/150‑1 (MQ=255)
                                                                                                           tACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGa                                          >  1:1481929/1‑150 (MQ=255)
                                                                                                             cTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAtt                                        <  1:1978876/150‑1 (MQ=255)
                                                                                                             atAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAtt                                        <  2:432329/149‑1 (MQ=255)
                                                                                                                aaGCTTTATTCGCTTTATAAGCCTGGATAGCTGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAttgtt                                     <  2:285578/150‑1 (MQ=255)
                                                                                                                aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAttgtt                                     <  1:54119/150‑1 (MQ=255)
                                                                                                                aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAttgtt                                     <  1:1199700/150‑1 (MQ=255)
                                                                                                                aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAttgtt                                     <  1:1772554/150‑1 (MQ=255)
                                                                                                                aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAttgtt                                     <  1:1144748/150‑1 (MQ=255)
                                                                                                                  gCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGa                                   <  2:505167/150‑1 (MQ=255)
                                                                                                                  gCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCAAAAGATTGTTGa                                   >  2:372716/1‑150 (MQ=255)
                                                                                                                    tttATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATa                                 >  2:231891/1‑150 (MQ=255)
                                                                                                                     ttATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATAc                                <  1:44704/150‑1 (MQ=255)
                                                                                                                         tCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGaa                            >  1:185268/1‑150 (MQ=255)
                                                                                                                         tCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGaa                            >  2:542116/1‑150 (MQ=255)
                                                                                                                          cgcgTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCATGATTCGCTAACGGCAATCGACGTTTATAATCTTCTAAAGATTGTTGATACTGAAt                           >  2:598336/1‑150 (MQ=255)
                                                                                                                          cGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCTCTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAAt                           >  1:1895991/1‑150 (MQ=255)
                                                                                                                          cGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAAt                           >  1:734704/1‑150 (MQ=255)
                                                                                                                          cGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAAt                           >  1:435630/1‑150 (MQ=255)
                                                                                                                           gCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATa                          >  1:1981401/1‑150 (MQ=255)
                                                                                                                            cTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTTTAATCTTCTAAAGATTGTTGATACTGAATAc                         >  1:2181820/1‑150 (MQ=255)
                                                                                                                               tataAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTg                      >  2:593005/1‑150 (MQ=255)
                                                                                                                               tataAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTg                      >  2:670843/1‑150 (MQ=255)
                                                                                                                                      tagcaaaGCGGCATTGAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTACCGGCACTCGACGGTTATAATCTTCTAATGATTGTTGATACTGAATACTTGCTGAAGc               >  2:1806767/7‑150 (MQ=255)
                                                                                                                                      tagcaaaGCGGCATTGAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGc               >  2:1797813/7‑150 (MQ=255)
                                                                                                                                         gATAGCGGCATTCAATGCCGCTTTGCTACTTATTAAAGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGAc            <  1:692647/150‑1 (MQ=255)
                                                                                                                                         caaaGCGGCATTGAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGAc            >  2:1419356/4‑150 (MQ=255)
                                                                                                                                          aaaGCGGCATTGAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGCAGCGACt           >  2:762592/3‑150 (MQ=255)
                                                                                                                                          aTAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACt           >  1:1036370/1‑150 (MQ=255)
                                                                                                                                          aTAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTAGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACt           <  2:1221662/150‑1 (MQ=255)
                                                                                                                                            aGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTc         >  1:1668243/1‑150 (MQ=255)
                                                                                                                                            aGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTc         <  2:1466432/150‑1 (MQ=255)
                                                                                                                                             gCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTGTTTTGAAATAACCCCCTGCTTCGCTAACGGCCCTCGACGGTTATAATCTTCTAATGATTGTTGATACTGAATACTTGCTGAAGCGACTTCg        >  2:422710/1‑150 (MQ=255)
                                                                                                                                              cGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCgg       >  1:1675974/1‑150 (MQ=255)
                                                                                                                                               ggCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAATTAACCCGCTGCTTCGCTAACCGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCAACTTCGGc      <  2:602076/150‑1 (MQ=255)
                                                                                                                                                   ttCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTg  <  1:1295732/150‑1 (MQ=255)
                                                                                                                                                   ttCAATGCAGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTg  <  2:1072050/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCGGTAACCGGACTCTTAATATCCGTACGTTTAAGCGCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTG  >  NC_000913/2482690‑2482986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: