Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,503,518 G→T 5.0% A323D (GCC→GAC) ‡ fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,503,5180GT 5.0% 362.3 / 5.3 117A323D (GCC→GAC) ‡fryAputative PTS enzyme: Hpr, enzyme I and IIA components
Reads supporting (aligned to +/- strand):  ref base G (52/57);  new base T (3/4);  total (56/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTGGG  >  NC_000913/2503369‑2503667
                                                                                                                                                     |                                                                                                                                                     
gggAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCgg                                                                                                                                                       <  1:1484016/150‑1 (MQ=255)
  gAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCa                                                                                                                                                     <  2:1119423/150‑1 (MQ=255)
       ggCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACg                                                                                                                                                >  1:1083208/1‑150 (MQ=255)
       ggCCAGCTGCGAGGCAGACTGCGCCTGTTGTTTTTGTCGTTTATCCGCCAGCGACTGCGCGAACTGATAATAACCGCTCACCGCGTCAGTCGTGTTAATCGACAGCACGCCGCACTGTGCGTCAAGGACTGCAGTTTGCCCGGCAGAACg                                                                                                                                                >  1:290259/1‑150 (MQ=255)
        gCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACgg                                                                                                                                               <  1:1698707/150‑1 (MQ=255)
        gCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTCTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACgg                                                                                                                                               >  2:2087143/1‑150 (MQ=255)
          cAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGc                                                                                                                                             <  2:1151132/150‑1 (MQ=255)
           aGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGTTATGCCGGGCa                                                                                                                                            <  1:808506/150‑1 (MQ=255)
              tGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGATTGCCCGTTATGCCGGGCaacc                                                                                                                                         <  1:262598/150‑3 (MQ=255)
               gcgcGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGTTATGCCGGGCaacct                                                                                                                                        <  2:2101249/150‑4 (MQ=255)
                cgcgGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGc                                                                                                                                       <  2:1715198/150‑1 (MQ=255)
                    gCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATACCGGGCAATCGCATcc                                                                                                                                   >  1:1640690/1‑150 (MQ=255)
                    gCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATcc                                                                                                                                   <  1:1012319/150‑1 (MQ=255)
                    gCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATcc                                                                                                                                   <  2:454330/150‑1 (MQ=255)
                      aGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAg                                                                                                                                 >  1:1739546/1‑150 (MQ=255)
                        ccTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCAAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCg                                                                                                                               >  2:2065655/1‑150 (MQ=255)
                         cTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCgg                                                                                                                              <  1:1547284/150‑1 (MQ=255)
                         cTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCGGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCgg                                                                                                                              <  1:1344284/150‑1 (MQ=255)
                          ggctccTGTTGTTTTTGGCGGTTATCCGCCAGCGTCTGCGCGACCTGATACTAACCGCGCACCGCGTCATTCGGGTTAATCGCCCGCACGCCGCACTGGGCGGCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGc                                                                                                                             <  1:1048639/146‑1 (MQ=255)
                           gcgcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCa                                                                                                                            >  2:1742404/1‑150 (MQ=255)
                           gcgcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCa                                                                                                                            >  2:1590011/1‑150 (MQ=255)
                             gcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAg                                                                                                                          >  1:1477409/1‑150 (MQ=255)
                                tgttgtTATTGCAGATTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCGCACCGCGTCAATCGGGTTAATCGCCAGCACCCCGCACTGGGCGTCAAGCCCTGCAGGTTGCCCGCCATAACGGGCAATCGCATCCAGCGGCAAGACa                                                                                                                       >  2:1292453/1‑150 (MQ=255)
                                  ttgttTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACt                                                                                                                     <  1:1065847/150‑1 (MQ=255)
                                   tgttTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTc                                                                                                                    <  2:864130/150‑1 (MQ=255)
                                    gttTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTcc                                                                                                                   >  2:967367/1‑149 (MQ=255)
                                    gttTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCa                                                                                                                   >  2:677706/1‑150 (MQ=255)
                                      ttttGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGt                                                                                                                 >  1:53730/1‑150 (MQ=255)
                                      accagccGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGt                                                                                                                 <  2:2136095/144‑1 (MQ=255)
                                       tttGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTa                                                                                                                >  1:568727/1‑150 (MQ=255)
                                          ggCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCg                                                                                                             >  2:834088/1‑150 (MQ=255)
                                            cGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCggg                                                                                                           >  1:1693502/1‑150 (MQ=255)
                                               ttATCCGCCTGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATc                                                                                                        <  2:1941063/150‑1 (MQ=255)
                                                tATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCg                                                                                                       >  2:689365/1‑150 (MQ=255)
                                                  tCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGcc                                                                                                     >  2:726588/1‑150 (MQ=255)
                                                    cGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGa                                                                                                   >  1:987435/1‑150 (MQ=255)
                                                    cGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGACAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATACAGCGGCAAGCCACTCAGTACCGGGATCGCCgc                                                                                                   >  2:1126037/1‑149 (MQ=255)
                                                     gCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAg                                                                                                  <  1:1264239/150‑1 (MQ=255)
                                                     gCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAg                                                                                                  <  2:539285/150‑1 (MQ=255)
                                                       cAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGc                                                                                                <  2:158485/150‑1 (MQ=255)
                                                        aGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCa                                                                                               >  2:824373/1‑150 (MQ=255)
                                                          cGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACg                                                                                             >  2:891648/1‑150 (MQ=255)
                                                              tGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGcc                                                                                         <  2:1871400/150‑1 (MQ=255)
                                                               gcgcGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACGGGGCGTCAAGCACTGCAGGTGGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCa                                                                                        <  1:1185477/150‑1 (MQ=255)
                                                                cgcgACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAg                                                                                       <  2:316583/150‑1 (MQ=255)
                                                                  cgACCTGATAATATCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGaa                                                                                     <  2:1392173/150‑1 (MQ=255)
                                                                   gACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAAt                                                                                    <  1:1835904/150‑1 (MQ=255)
                                                                     ccTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCa                                                                                  >  1:1606439/1‑150 (MQ=255)
                                                                      cTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAg                                                                                 <  2:241702/150‑1 (MQ=255)
                                                                      cTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAg                                                                                 >  2:254645/1‑150 (MQ=255)
                                                                       tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCCCGGGCCAGCATCTgt                                                                                >  2:348564/1‑150 (MQ=255)
                                                                       tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                >  1:1250480/1‑150 (MQ=255)
                                                                        gATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgtg                                                                               <  2:817032/150‑1 (MQ=255)
                                                                           aataaCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGGCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTAt                                                                            <  1:185927/150‑1 (MQ=255)
                                                                            ataaCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGCTCGCCGCGGCCCGGGCCAGAATCAGTGTATg                                                                           >  2:233515/1‑150 (MQ=255)
                                                                            ataaCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATg                                                                           <  1:1904306/150‑1 (MQ=255)
                                                                             taaCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCACTCGCCTCCAGCGGCACGCCACTCAGTACCGGGATCGCCGAGGCGCGGGCCAGAATCAGTGTATGc                                                                          >  2:1144419/1‑150 (MQ=255)
                                                                             taaCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGc                                                                          >  2:959359/1‑150 (MQ=255)
                                                                              aaCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCCGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGCATCAGTCTATGCg                                                                         >  1:1386810/1‑150 (MQ=255)
                                                                               aCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGa                                                                        <  1:1788422/150‑1 (MQ=255)
                                                                               aCCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGa                                                                        <  1:553970/150‑1 (MQ=255)
                                                                                   cTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCACTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTg                                                                    >  1:2165152/1‑150 (MQ=255)
                                                                                      aCCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGACAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTgcgc                                                                 >  2:1470227/1‑150 (MQ=255)
                                                                                       ccGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATTGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGcc                                                                >  2:1508369/1‑150 (MQ=255)
                                                                                         gcgTCATTCGGGTTAATCGCCAGCACGCCGGACTGTGCGGCAAGCACTGCAGTATGCCCGTCATAACGGGCAATCGCATCAAGCGGCAAGGCACTCAGTAGCGGGATCGCCGAGGCACGTGCCAGAATCAGTGTAAGCGAGGTGCGCCCg                                                              <  2:332669/150‑1 (MQ=255)
                                                                                          cgTCATTCGGCGCACTCGCCAGCCCGCCGCACTGGGCGGCAAGCACAGCAGGTTGCCCGGCATAACGGGCAAGCGCACCCCGCGGCAAGCCACTCCGTACCGGGCGCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCgg                                                             <  1:2027350/150‑1 (MQ=255)
                                                                                           gTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGt                                                            <  2:1739546/150‑1 (MQ=255)
                                                                                             cATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGttt                                                          <  2:839395/150‑1 (MQ=255)
                                                                                             cATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGGCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGttt                                                          <  1:601332/150‑1 (MQ=255)
                                                                                               ttCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATGACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtc                                                        <  2:538291/150‑1 (MQ=255)
                                                                                                    gctAATCGCCAGCCCGCCGCACTGGCCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAg                                                   <  1:292359/148‑1 (MQ=255)
                                                                                                       aaTCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAAt                                                >  2:652611/1‑150 (MQ=255)
                                                                                                       aaTCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAAt                                                <  2:1420133/150‑1 (MQ=255)
                                                                                                         tCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCa                                              <  2:2154242/150‑1 (MQ=255)
                                                                                                           gCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATg                                            >  2:1868178/1‑150 (MQ=255)
                                                                                                           gCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTGTTCTCCAGAATCATg                                            >  1:1113957/1‑150 (MQ=255)
                                                                                                             cAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGcc                                          <  1:456816/150‑1 (MQ=255)
                                                                                                               gCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCg                                        <  2:762555/150‑1 (MQ=255)
                                                                                                                       cACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGAttt                                >  2:1234691/1‑150 (MQ=255)
                                                                                                                         cTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGAttttt                              >  1:777552/1‑150 (MQ=255)
                                                                                                                          tGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTc                             <  1:1559885/150‑1 (MQ=255)
                                                                                                                           gggCGTCAAGCACTGCAGGTTGCCCGGCATACCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCa                            <  2:655526/150‑1 (MQ=255)
                                                                                                                           gggCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCa                            <  2:1783532/150‑1 (MQ=255)
                                                                                                                             gCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCaaa                          <  2:368083/150‑1 (MQ=255)
                                                                                                                             gCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCaaa                          <  1:891648/150‑1 (MQ=255)
                                                                                                                                    gCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGc                   <  1:2087143/150‑1 (MQ=255)
                                                                                                                                    gCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGc                   <  2:182817/150‑1 (MQ=255)
                                                                                                                                         gCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCATTGCTCaaa              >  2:623766/1‑150 (MQ=255)
                                                                                                                                         gCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaaa              >  2:1759198/1‑150 (MQ=255)
                                                                                                                                          cAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaaaa             <  2:616733/150‑1 (MQ=255)
                                                                                                                                           cgattGCCCGTTATGCCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaaaaa            >  2:652368/4‑150 (MQ=255)
                                                                                                                                           cgattGCCCGTTATGCCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaaaaa            >  2:2169878/4‑150 (MQ=255)
                                                                                                                                           cgattGCCCGTTATGCCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGACCGCAAGATTTTTCAAATCAAGGCTCaaaaa            >  2:1512326/4‑150 (MQ=255)
                                                                                                                                            ggTTGCCCGGCATAACGGGCAATAGCATCCAGAGGCAAGACACTCATTTCCGGCATAGCCGCGTCACGGGCCTGAATCAGTGTATGCGAGGTGCGACCGGATTTCTACCGAATCATGCCCGCAACAATTTTCAAATCAAGGCTCAAAAAc           >  2:1186025/1‑150 (MQ=255)
                                                                                                                                              ttGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTg         <  1:1700802/150‑1 (MQ=255)
                                                                                                                                              ttGCCCGGCATAACGGGAAATCGAATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTg         <  2:2056401/150‑1 (MQ=255)
                                                                                                                                                gCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCTAATCAAGGCTCAAAAACTGGc       >  1:634504/1‑150 (MQ=255)
                                                                                                                                                 cccGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCt      >  2:1912027/1‑150 (MQ=255)
                                                                                                                                                    ggCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGACTCAAAAACTGGCTTgg   >  2:545582/1‑150 (MQ=255)
                                                                                                                                                     gCATAACGGGCAATCGCTTCCAGGGGCAATCCACTCAGTACTGGGATAGCCGAGGCACGGGCCATAATCAGTGTATGCGAGGTTCGCCCGGTTTTCTCCATAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAACACTGGCTTggg  <  2:126934/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTGGG  >  NC_000913/2503369‑2503667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑