Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,507,109 T→G 5.3% A262A (GCA→GCC) ‡ fryC ← putative enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,507,1090TG 5.3% 411.0 / 9.7 131A262A (GCA→GCC) ‡fryCputative enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base T (59/65);  new base G (3/4);  total (62/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 133 total aligned reads displayed.

AAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTA  >  NC_000913/2506962‑2507258
                                                                                                                                                   |                                                                                                                                                     
aaGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGc                                                                                                                                                     <  2:255471/150‑1 (MQ=255)
 aGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCg                                                                                                                                                    >  2:226353/1‑150 (MQ=255)
  gTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGGCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCgg                                                                                                                                                   <  2:144961/150‑1 (MQ=255)
    tGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACATGCGGCt                                                                                                                                                 >  2:333961/1‑150 (MQ=255)
      aGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCttt                                                                                                                                               >  1:1275985/1‑150 (MQ=255)
       gCGTTGAACAGGCGTTTGCCGGTTAAACGACGGGCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAAAAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTttg                                                                                                                                              <  2:1631978/150‑1 (MQ=255)
        cGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCTATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATTGAGATTGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTttgt                                                                                                                                             <  1:525648/150‑1 (MQ=255)
         gTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgtt                                                                                                                                            <  2:28162/150‑1 (MQ=255)
         gTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGGCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTttgtt                                                                                                                                            <  1:1941838/150‑1 (MQ=255)
          ttGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTttgttg                                                                                                                                           <  2:1880384/150‑1 (MQ=255)
           tGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgttgc                                                                                                                                          <  1:962689/150‑2 (MQ=255)
           tGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGa                                                                                                                                          <  1:2085914/150‑1 (MQ=255)
            gAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATACCGCTAGCGATAGAACGAGCGGGGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTGTGTTGAt                                                                                                                                         >  1:309619/1‑150 (MQ=255)
            gAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCTGCTTTGTTCAt                                                                                                                                         >  1:2078417/1‑150 (MQ=255)
             aaCAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATATCGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTCTTGaac                                                                                                                                        >  1:2050929/1‑148 (MQ=255)
             aaCAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgttgcct                                                                                                                                        <  2:2065715/150‑4 (MQ=255)
             aaCAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATc                                                                                                                                        <  2:908618/150‑1 (MQ=255)
             aaCAGGCGTTTGCCGCTTAAACGACGGTCGATAATGGGCGCCAGCCCCAGACCAATCGGCGGAATAACCAAAGCGATAGAACGAGCGTTGACTGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgttgcct                                                                                                                                        <  2:366934/150‑4 (MQ=255)
               cAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCgg                                                                                                                                      >  2:1763444/1‑150 (MQ=255)
                 ggCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTAGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGc                                                                                                                                    >  1:1342548/1‑150 (MQ=255)
                 ggCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTAAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGc                                                                                                                                    >  1:209760/1‑150 (MQ=255)
                      ttGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGccacca                                                                                                                               <  1:752628/150‑1 (MQ=255)
                        gCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAg                                                                                                                             >  2:1038772/1‑150 (MQ=255)
                         ccGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAgg                                                                                                                            >  2:929156/1‑150 (MQ=255)
                               aaaCGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGAtt                                                                                                                      >  1:1523017/1‑150 (MQ=255)
                                aaCGACGGTCGATTATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGGGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTTAATCTGAAGTCAACATAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTg                                                                                                                     <  2:1945839/150‑1 (MQ=255)
                                 acgacgGTCGATAATGGTCGCCAGCCCCAGACGAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTTGTCTGTGGTAAAGCTGAAGGCAACATTACCTGCGGCTTGGTTGATCGGGCCACCAAGGTCGATTGc                                                                                                                    <  2:306394/150‑1 (MQ=255)
                                 acgacgGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGc                                                                                                                    <  1:2201552/150‑1 (MQ=255)
                                 acgacgGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGc                                                                                                                    <  1:1616806/150‑1 (MQ=255)
                                   gacgGTCGCTAATGGGCGCCAGCCCCAGACCAATCGGCGGCATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTg                                                                                                                  <  1:1393120/150‑1 (MQ=255)
                                   gacgGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTg                                                                                                                  <  2:2086273/150‑1 (MQ=255)
                                    acgGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGt                                                                                                                 >  1:2160790/1‑150 (MQ=255)
                                    acgGTCGATAATGGTAGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGt                                                                                                                 >  1:134081/1‑150 (MQ=255)
                                      ggTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATACCGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTcg                                                                                                               <  2:85218/150‑1 (MQ=255)
                                       gTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGAAAGCGATAGAACGAGCGGTGACCGGTAATACGTTGTCAGTGGTAAAGCTGAAGGCAACAAAACCTTCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTcgc                                                                                                              <  1:1317562/150‑1 (MQ=255)
                                             aaTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggc                                                                                                        <  2:1751879/150‑1 (MQ=255)
                                             aaTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggc                                                                                                        <  2:1668660/150‑1 (MQ=255)
                                              aTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGCACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggcg                                                                                                       >  1:1485862/1‑150 (MQ=255)
                                              aTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggcg                                                                                                       <  1:1524055/150‑1 (MQ=255)
                                              aTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggcg                                                                                                       >  1:662915/1‑150 (MQ=255)
                                              aTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggcg                                                                                                       >  1:2029983/1‑150 (MQ=255)
                                                  gcGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATACAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATcc                                                                                                   <  1:1880164/149‑1 (MQ=255)
                                                      cAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTAGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCAt                                                                                               <  2:1342548/150‑1 (MQ=255)
                                                        gCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGGCAGTGGTAAATCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTg                                                                                             <  2:1509641/150‑1 (MQ=255)
                                                          cccAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCg                                                                                           >  2:745905/1‑150 (MQ=255)
                                                             aGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTAc                                                                                        <  2:2162235/150‑1 (MQ=255)
                                                             aGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTAc                                                                                        <  2:1015812/150‑1 (MQ=255)
                                                             aGAACAATCGGCGGAATAACGATAGCGATAGAACGAGCGGAGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGGCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTAc                                                                                        <  2:1392507/150‑1 (MQ=255)
                                                              gcccAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACa                                                                                       <  1:1375038/148‑1 (MQ=255)
                                                                ccAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATc                                                                                     >  2:64427/1‑150 (MQ=255)
                                                                  aaTCGGCGGAATTACGATAGCGATATAACGAGCGGTGACCGGTAATTCGTGGTCAGTGGTAAAGCTGTAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAg                                                                                   <  1:857495/150‑1 (MQ=255)
                                                                        cggAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGcc                                                                             >  1:1688632/1‑150 (MQ=255)
                                                                         ggAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGccc                                                                            <  1:859769/150‑1 (MQ=255)
                                                                             tAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtct                                                                        <  2:134081/150‑1 (MQ=255)
                                                                             aaaCGATAGCGATAGAACGAGCGGTGACCGGTAATATGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtct                                                                        <  2:1636775/149‑1 (MQ=255)
                                                                                cgatagcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCAc                                                                     >  1:1946338/1‑150 (MQ=255)
                                                                                 gatagcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCAcc                                                                    <  2:2119328/150‑1 (MQ=255)
                                                                                    agcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGc                                                                 <  2:2102876/150‑1 (MQ=255)
                                                                                     gcgatagAACGAGCGGGGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCg                                                                <  1:700174/150‑1 (MQ=255)
                                                                                      cgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCgg                                                               <  2:2113577/150‑1 (MQ=255)
                                                                                      cgatagAACGAGCGGGGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCGTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCgg                                                               <  1:927246/150‑1 (MQ=255)
                                                                                       gatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGc                                                              <  1:1038772/150‑1 (MQ=255)
                                                                                        atagAACGAGCGGTGACCGGTATTACGTGGTCAGTGGTAACGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCa                                                             <  1:499189/150‑1 (MQ=255)
                                                                                        atagAACGAGCGGTGACCGGTAATACGTGGGCAGTGGGAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCa                                                             <  2:924403/150‑1 (MQ=255)
                                                                                          agAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGt                                                           <  2:2034212/150‑1 (MQ=255)
                                                                                           gAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTc                                                          <  2:1548827/150‑1 (MQ=255)
                                                                                            aaCGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCa                                                         <  2:20539/150‑1 (MQ=255)
                                                                                              cGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGc                                                       >  1:497500/1‑150 (MQ=255)
                                                                                                    gTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTa                                                 >  1:2149379/1‑150 (MQ=255)
                                                                                                      gACCGGTAATACGTGGTCAGTGGTAAAGCTGACGGCAACAAAACCTGCGGCGTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACg                                               <  1:1098192/150‑1 (MQ=255)
                                                                                                      gACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACg                                               >  1:462514/1‑150 (MQ=255)
                                                                                                          ggTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATa                                           >  1:1735340/1‑150 (MQ=255)
                                                                                                            tAATACGTGGTCAGTGGTAAAGATGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGGCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATAcc                                         <  1:1387462/150‑1 (MQ=255)
                                                                                                             aaTACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATAccg                                        >  1:779864/1‑150 (MQ=255)
                                                                                                                 cGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGt                                    >  2:868006/1‑150 (MQ=255)
                                                                                                                 cGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGt                                    >  2:1149772/1‑150 (MQ=255)
                                                                                                                   tcgTCAGTGGTACAGCTGAAGGCCACACAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCAATGCGTCCATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTg                                  <  2:1374195/148‑1 (MQ=255)
                                                                                                                       cAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGGCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATcc                              <  2:1473932/150‑1 (MQ=255)
                                                                                                                           ggTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGcc                          <  1:2067598/150‑1 (MQ=255)
                                                                                                                              aaaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGTCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccacc                       <  2:1556042/150‑1 (MQ=255)
                                                                                                                              aaaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccacc                       <  1:1516583/150‑1 (MQ=255)
                                                                                                                              aaaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccacc                       >  1:721995/1‑150 (MQ=255)
                                                                                                                               aaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCCGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccacca                      >  1:33008/1‑150 (MQ=255)
                                                                                                                                 gCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCaaa                    >  2:974359/1‑150 (MQ=255)
                                                                                                                                 gCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCaaa                    >  1:1916505/1‑150 (MQ=255)
                                                                                                                                   tGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg                  >  1:431077/1‑150 (MQ=255)
                                                                                                                                     cgatcaacaaAGCCGCAGGTTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCCCCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgggg                >  2:1517423/5‑150 (MQ=255)
                                                                                                                                        gCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGtg             >  2:1894582/1‑150 (MQ=255)
                                                                                                                                         caacaaAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACAGCCGTTGATACAGCCACCAAAGGGGGtga            >  1:592065/1‑150 (MQ=255)
                                                                                                                                         caacaaAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGGCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCCGCACAGTACGGATACCGCCGTTGCTCCAGCCACCAAAGGGGGtga            >  1:406553/1‑150 (MQ=255)
                                                                                                                                          aacaaAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGtgat           >  2:1733896/1‑150 (MQ=255)
                                                                                                                                           acaaAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGtgatg          <  1:1578029/150‑1 (MQ=255)
                                                                                                                                             aaaaCCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGAc        <  2:1097785/150‑1 (MQ=255)
                                                                                                                                             aaaaCCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCACCCACCAAAGGGGGggatgcc        >  1:1124023/1‑148 (MQ=255)
                                                                                                                                              aaaCCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGCTCCAGCCACCAAAGGGGGTGATGACg       >  1:1148915/1‑150 (MQ=255)
                                                                                                                                              aaaCCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACg       <  1:1763444/150‑1 (MQ=255)
                                                                                                                                              aaaCCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACg       >  1:1173096/1‑150 (MQ=255)
                                                                                                                                               acccTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCAGGCGCCGCGGCGATCGCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgt      <  1:333961/148‑1 (MQ=255)
                                                                                                                                                   tGCGGCTTTGTTGATCGGTCCACCAAGGTCTATTGCTGGCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtagta  <  2:2078417/150‑1 (MQ=255)
                                                                                                                                                   tGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtagta  <  2:686444/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTA  >  NC_000913/2506962‑2507258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: