Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,507,111 C→A 5.4% A262S (GCA→TCA) ‡ fryC ← putative enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,507,1110CA 5.4% 390.5 / 9.4 131A262S (GCA→TCA) ‡fryCputative enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base C (58/66);  new base A (3/4);  total (61/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

Only 100 of 134 total aligned reads displayed.

AAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTA  >  NC_000913/2506962‑2507258
                                                                                                                                                     |                                                                                                                                                   
aaGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGc                                                                                                                                                     <  2:255471/150‑1 (MQ=255)
aaGTTGAGAGTTGAACAGGCGTTTGACTGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAATCTCAAGGCAACAAAACCTGc                                                                                                                                                     >  1:93701/1‑150 (MQ=255)
    tGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACATGCGGCt                                                                                                                                                 >  2:333961/1‑150 (MQ=255)
      aGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCttt                                                                                                                                               >  1:1275985/1‑150 (MQ=255)
        cGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCTATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATTGAGATTGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTttgt                                                                                                                                             <  1:525648/150‑1 (MQ=255)
         gTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGCACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTttgtt                                                                                                                                            >  2:2109507/1‑150 (MQ=255)
         gTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgtt                                                                                                                                            <  2:28162/150‑1 (MQ=255)
          ttGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTttgttg                                                                                                                                           <  1:718666/150‑1 (MQ=255)
            gAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATACCGCTAGCGATAGAACGAGCGGGGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTGTGTTGAt                                                                                                                                         >  1:309619/1‑150 (MQ=255)
            gAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCTGCTTTGTTCAt                                                                                                                                         >  1:2078417/1‑150 (MQ=255)
             aaCAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgttgcct                                                                                                                                        <  2:2065715/150‑4 (MQ=255)
             aaCAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATc                                                                                                                                        <  2:908618/150‑1 (MQ=255)
             aaCAGGCGTTTGCCGCTTAAACGACGGTCGATAATGGGCGCCAGCCCCAGACCAATCGGCGGAATAACCAAAGCGATAGAACGAGCGTTGACTGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgttgcct                                                                                                                                        <  2:366934/150‑4 (MQ=255)
               cAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCgg                                                                                                                                      >  2:1763444/1‑150 (MQ=255)
                 ggCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTAGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGc                                                                                                                                    >  1:1342548/1‑150 (MQ=255)
                 ggCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTAAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGc                                                                                                                                    >  1:209760/1‑150 (MQ=255)
                        gCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAg                                                                                                                             >  2:1038772/1‑150 (MQ=255)
                         ccGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAgg                                                                                                                            >  2:929156/1‑150 (MQ=255)
                                aaCGACGGTCGATTATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGGGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTTAATCTGAAGTCAACATAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTg                                                                                                                     <  2:1945839/150‑1 (MQ=255)
                                aaCGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTg                                                                                                                     >  2:1561437/1‑150 (MQ=255)
                                aaCGACGGTCGATAATGGTCGCCAGCCACAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTg                                                                                                                     >  1:551563/1‑150 (MQ=255)
                                 acgacgGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGc                                                                                                                    <  1:2201552/150‑1 (MQ=255)
                                   gacgGTCGCTAATGGGCGCCAGCCCCAGACCAATCGGCGGCATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTg                                                                                                                  <  1:1393120/150‑1 (MQ=255)
                                   gacgGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTg                                                                                                                  <  2:2086273/150‑1 (MQ=255)
                                   gacgGTCGATAATGGTAGCCAGCCCCAGACCAAGCGGCGGAATACCGATAGCGATTGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTg                                                                                                                  <  1:1385733/150‑1 (MQ=255)
                                    acgGTCGATAATGGTAGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGt                                                                                                                 >  1:134081/1‑150 (MQ=255)
                                           aTAATGGTCTCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCcgcg                                                                                                          <  2:1414094/150‑1 (MQ=255)
                                             aaTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggc                                                                                                        <  2:1751879/150‑1 (MQ=255)
                                             aaTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggc                                                                                                        <  2:1668660/150‑1 (MQ=255)
                                              aTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGCACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggcg                                                                                                       >  1:1485862/1‑150 (MQ=255)
                                              aTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggcg                                                                                                       >  1:2029983/1‑150 (MQ=255)
                                              aTGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCgcggcg                                                                                                       <  1:1524055/150‑1 (MQ=255)
                                                  tCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGGCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAACGGCGATTGCTGTCGCCGCGGCGATcc                                                                                                   >  1:384868/1‑150 (MQ=255)
                                                  gcGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATACAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATcc                                                                                                   <  1:1880164/149‑1 (MQ=255)
                                                   cGCCAGCCCCAGACCAATCGGCTGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTAGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATccc                                                                                                  >  1:1327864/1‑150 (MQ=255)
                                                     ccAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGCAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCGTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCa                                                                                                >  1:469206/1‑150 (MQ=255)
                                                      cAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCaa                                                                                               >  2:1839098/1‑149 (MQ=255)
                                                        gCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGGCAGTGGTAAATCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTg                                                                                             <  2:1509641/150‑1 (MQ=255)
                                                          cccAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCg                                                                                           >  2:745905/1‑150 (MQ=255)
                                                           ccAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTGTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGCTCCCCATTGCGt                                                                                          >  1:1974858/1‑150 (MQ=255)
                                                            cAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTa                                                                                         >  2:1516380/1‑150 (MQ=255)
                                                             aGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTAc                                                                                        <  2:1015812/150‑1 (MQ=255)
                                                             aGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTAc                                                                                        <  2:2162235/150‑1 (MQ=255)
                                                             aGAACAATCGGCGGAATAACGATAGCGATAGAACGAGCGGAGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGGCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTAc                                                                                        <  2:1392507/150‑1 (MQ=255)
                                                                       gcggAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAgcgcgc                                                                              >  1:1892394/1‑150 (MQ=255)
                                                                        cggAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGcc                                                                             >  1:1688632/1‑150 (MQ=255)
                                                                         ggAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGccc                                                                            <  1:859769/150‑1 (MQ=255)
                                                                             tAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtct                                                                        >  1:829941/1‑150 (MQ=255)
                                                                             tAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtct                                                                        <  2:134081/150‑1 (MQ=255)
                                                                             aaaCGATAGCGATAGAACGAGCGGTGACCGGTAATATGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtct                                                                        <  2:1636775/149‑1 (MQ=255)
                                                                              aaCGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtctc                                                                       <  1:1561437/150‑1 (MQ=255)
                                                                              aaCGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtctc                                                                       <  1:1506968/150‑1 (MQ=255)
                                                                              aaCGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtctc                                                                       >  2:1039748/1‑150 (MQ=255)
                                                                               aCGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCCCCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCa                                                                      >  2:592225/1‑150 (MQ=255)
                                                                               aCGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCa                                                                      <  1:1731800/150‑1 (MQ=255)
                                                                                cgatagcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCAc                                                                     >  1:1946338/1‑150 (MQ=255)
                                                                                cgatagcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCAc                                                                     >  2:769355/1‑150 (MQ=255)
                                                                                 gatagcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCAcc                                                                    <  1:226353/150‑1 (MQ=255)
                                                                                 gatagcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCAcc                                                                    <  2:2119328/150‑1 (MQ=255)
                                                                                    agcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGc                                                                 <  2:2102876/150‑1 (MQ=255)
                                                                                     gcgatagAACGAGCGGGGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCg                                                                <  1:700174/150‑1 (MQ=255)
                                                                                      cgatagAACGAGCGGGGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCGTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCgg                                                               <  1:927246/150‑1 (MQ=255)
                                                                                       gatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGc                                                              <  1:1038772/150‑1 (MQ=255)
                                                                                           gAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTc                                                          <  2:2076261/150‑1 (MQ=255)
                                                                                           gAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTc                                                          <  2:1548827/150‑1 (MQ=255)
                                                                                            aaCGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCa                                                         <  2:20539/150‑1 (MQ=255)
                                                                                              cGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGc                                                       >  1:497500/1‑150 (MQ=255)
                                                                                                    gTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTa                                                 >  1:2149379/1‑150 (MQ=255)
                                                                                                      gACCGGTAATACGTGGTCAGTGGTAAAGCTGACGGCAACAAAACCTGCGGCGTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACg                                               <  1:1098192/150‑1 (MQ=255)
                                                                                                      gACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACg                                               >  1:462514/1‑150 (MQ=255)
                                                                                                          ggTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATa                                           >  1:1735340/1‑150 (MQ=255)
                                                                                                            tAATACGTGGTCAGTGGTAAAGATGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGGCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATAcc                                         <  1:1387462/150‑1 (MQ=255)
                                                                                                             aaTACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATAccg                                        >  1:779864/1‑150 (MQ=255)
                                                                                                             aaTACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTCGTTGATCGGGCCACCAAGGTCGAGTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATAccg                                        <  1:1152053/150‑1 (MQ=255)
                                                                                                                 cGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGt                                    >  2:1149772/1‑150 (MQ=255)
                                                                                                                 cGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGt                                    >  2:868006/1‑150 (MQ=255)
                                                                                                                 cGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGACGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGt                                    >  2:921440/1‑150 (MQ=255)
                                                                                                                   tcgTCAGTGGTACAGCTGAAGGCCACACAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCAATGCGTCCATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTg                                  <  2:1374195/148‑1 (MQ=255)
                                                                                                                       cAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGGCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATcc                              <  2:1473932/150‑1 (MQ=255)
                                                                                                                           ggTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGcc                          <  1:2067598/150‑1 (MQ=255)
                                                                                                                             tAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccac                        >  1:2075972/1‑150 (MQ=255)
                                                                                                                              aaaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGTCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccacc                       <  2:1556042/150‑1 (MQ=255)
                                                                                                                              aaaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccacc                       <  1:1516583/150‑1 (MQ=255)
                                                                                                                              aaaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccacc                       >  1:721995/1‑150 (MQ=255)
                                                                                                                               aaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGccacca                      <  1:2108432/150‑1 (MQ=255)
                                                                                                                                 gCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCaaa                    >  1:1916505/1‑150 (MQ=255)
                                                                                                                                 gCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCaaa                    >  2:974359/1‑150 (MQ=255)
                                                                                                                                   cccgatcaacaaAGCCGCAGGTTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg                  >  2:2203344/7‑150 (MQ=255)
                                                                                                                                        gCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGtg             <  2:2029983/150‑1 (MQ=255)
                                                                                                                                        gCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGtg             >  2:1894582/1‑150 (MQ=255)
                                                                                                                                         caacaaAGCCGCAGGTTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGACCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGtga            >  2:1695391/1‑150 (MQ=255)
                                                                                                                                         caacaaAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATGCAGCCACCAAAGGGGGtga            >  2:293694/1‑150 (MQ=255)
                                                                                                                                         caacaaAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACAGCCGTTGATACAGCCACCAAAGGGGGtga            >  1:592065/1‑150 (MQ=255)
                                                                                                                                         caacaaAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGGCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCCGCACAGTACGGATACCGCCGTTGCTCCAGCCACCAAAGGGGGtga            >  1:406553/1‑150 (MQ=255)
                                                                                                                                           acaaAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGtgatg          <  1:1578029/150‑1 (MQ=255)
                                                                                                                                             aaaaCCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCACCCACCAAAGGGGGggatgcc        >  1:1124023/1‑148 (MQ=255)
                                                                                                                                              aaaCCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACg       >  1:1173096/1‑150 (MQ=255)
                                                                                                                                               acccTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCAGGCGCCGCGGCGATCGCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgt      <  1:333961/148‑1 (MQ=255)
                                                                                                                                                  cTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtagt   >  2:134152/1‑150 (MQ=255)
                                                                                                                                                   tGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtagta  <  2:686444/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTA  >  NC_000913/2506962‑2507258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: