Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,729 T→A 5.5% intergenic (‑23/‑313) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,7290TA 5.5% 401.1 / 7.5 137intergenic (‑23/‑313)mntH/nupCmanganese/divalent cation transporter/nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base T (77/52);  new base A (5/3);  total (82/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACA  >  NC_000913/2512580‑2512877
                                                                                                                                                     |                                                                                                                                                    
  atatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACGCTCAACGCGATAGTTCGGCATCTTGTGCCTCTAAACCATAGCCttt                                                                                                                                                    >  1:1646308/1‑150 (MQ=255)
    atTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGc                                                                                                                                                  <  1:1617263/150‑1 (MQ=255)
     ttGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCCACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCt                                                                                                                                                 >  1:427542/1‑150 (MQ=255)
       ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                               <  1:867797/150‑1 (MQ=255)
        gTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATg                                                                                                                                              >  1:359632/1‑150 (MQ=255)
        gTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATg                                                                                                                                              >  2:1417226/1‑150 (MQ=255)
         tCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGt                                                                                                                                             >  2:1845536/1‑150 (MQ=255)
          cGCAAAGTTACCGGGATAGATATAACCAATCACCGCAATGAACGCAGGGCCCATTAATGCGCGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                            <  2:92594/150‑1 (MQ=255)
           gCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGttt                                                                                                                                           <  1:478831/150‑1 (MQ=255)
              aaGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGttttag                                                                                                                                        <  2:330998/150‑4 (MQ=255)
               aGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATg                                                                                                                                       <  1:233501/150‑1 (MQ=255)
                gTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCACCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGttttagag                                                                                                                                      <  1:2141561/150‑6 (MQ=255)
                gTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGc                                                                                                                                      >  2:1825986/1‑150 (MQ=255)
                    actgtaTCGATATAACCAATCGCCGAAATGAACGCAGGAACCATTACTGCGAGCCTCATCTTGCGCGCCGCCCGTCCTGTTATACTCTCAACGCGCTAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATg                                                                                                                                  <  2:1048412/145‑1 (MQ=255)
                         aTCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTTCTCTCACCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTTCTATGTGTCATGCTATGCCaaa                                                                                                                             >  2:124028/1‑150 (MQ=255)
                            gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACga                                                                                                                          >  2:857054/1‑150 (MQ=255)
                            gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACga                                                                                                                          >  1:2043005/1‑150 (MQ=255)
                            gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCAGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTGTGCTATGTTTCATGCTATGCCAAACgc                                                                                                                          >  1:1134226/1‑149 (MQ=255)
                             atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         <  1:1253369/150‑1 (MQ=255)
                             atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         >  2:632928/1‑150 (MQ=255)
                             atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         >  1:2017114/1‑150 (MQ=255)
                             atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCATTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         >  2:1008947/1‑150 (MQ=255)
                               ataACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCTAGCCTCATCTTGCGCGACGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTTAAACATAGCCTTTGCTATGTTTCATGCTATGCCAATCGAGaa                                                                                                                       <  1:1269433/150‑1 (MQ=255)
                                taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                      <  1:175586/150‑1 (MQ=255)
                                   ccAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGAt                                                                                                                   <  1:23301/150‑1 (MQ=255)
                                    cAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGAtt                                                                                                                  >  1:538551/1‑150 (MQ=255)
                                    cAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCATCATCTTGCGCGACGCCCGTCCGCTGGTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGAtt                                                                                                                  <  1:1420520/150‑1 (MQ=255)
                                     aaTCGCCGCAATGATCGCAGGTGCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATTCTATGCCAAACGAGAATGATTa                                                                                                                 <  1:559926/150‑1 (MQ=255)
                                     aaTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTCGTGCCTCTAAAACATAGCCTTTGCTATGTTTCCTGCTATGCCAAACGAGAATGATTa                                                                                                                 >  2:1161873/1‑150 (MQ=255)
                                         gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaa                                                                                                             >  1:2090402/1‑150 (MQ=255)
                                              aaTGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCa                                                                                                        >  1:1912583/1‑150 (MQ=255)
                                                tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                      >  2:20359/1‑150 (MQ=255)
                                                tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                      <  2:1931618/150‑1 (MQ=255)
                                                 gAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAttt                                                                                                     >  1:448657/1‑150 (MQ=255)
                                                 gAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAttt                                                                                                     >  1:153639/1‑150 (MQ=255)
                                                     tcAGGTACCATTAATGCGAGCCTTATCTTGCGCTCCGCCCGTCATCTTCTACTCTCAACGCGATAGTTCGTCATCTTGTTCCTCTAAAACATAGCCTTTGCTATGTTTAATGCTATGCCAAACGATAATGATTATCAAATTCATTTAAAt                                                                                                 <  2:80056/149‑1 (MQ=255)
                                                      cAGGTCCCATTAATGCTAGCCTCATCTTGCGCGACGCACGTCCGCTGCTACTCTCACCGCGATAGTTCGGAATATTGTGCCTCTATAACATAGCCTTTGCTATGTTTCATGCTATGGCACACGAGAATGATTTTGATAATCAttatcgtt                                                                                                <  2:541574/150‑7 (MQ=255)
                                                      cAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATg                                                                                                <  2:153639/150‑1 (MQ=255)
                                                      cAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATg                                                                                                <  2:448657/150‑1 (MQ=255)
                                                         gTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTATCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGAt                                                                                             >  1:890916/1‑150 (MQ=255)
                                                           cccATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtg                                                                                           >  1:1028776/1‑150 (MQ=255)
                                                           cccATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtg                                                                                           >  1:427298/1‑150 (MQ=255)
                                                             cATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtgtg                                                                                         >  1:705111/1‑150 (MQ=255)
                                                              aTTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATATTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtgg                                                                                        <  2:1206601/150‑1 (MQ=255)
                                                               ttAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                       <  2:170400/150‑1 (MQ=255)
                                                                 aaTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGa                                                                                     >  1:961829/1‑150 (MQ=255)
                                                                    gCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAttt                                                                                  >  2:1421339/1‑150 (MQ=255)
                                                                       aGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATTATTATCAAATTCATTTAAATGGATTGTGGTGATTtctc                                                                               <  1:597955/150‑1 (MQ=255)
                                                                        gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTGTGCTATGTTTCCTGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                              >  1:1335390/1‑150 (MQ=255)
                                                                            cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATATTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                          <  2:1912583/150‑1 (MQ=255)
                                                                             aTCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATat                                                                         >  2:1250326/1‑149 (MQ=255)
                                                                             aTCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAg                                                                         >  2:493383/1‑150 (MQ=255)
                                                                             aTCTTGCGCGCCGCCCGTCCGCTGCTACTCACAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATATCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGACAg                                                                         <  2:1397697/150‑1 (MQ=255)
                                                                               cTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAc                                                                       >  2:1862595/1‑150 (MQ=255)
                                                                               cTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAc                                                                       >  2:231277/1‑150 (MQ=255)
                                                                                 tGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCa                                                                     >  1:1795946/1‑150 (MQ=255)
                                                                                    gcggcgCCCGTCCGCTGCTAATCTCATCGCGATATTTCGTCATCTTGTGCCTCTATAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCGAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGa                                                                  <  1:1295933/150‑1 (MQ=255)
                                                                                    gcgcCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGa                                                                  <  2:245998/150‑1 (MQ=255)
                                                                                      gccgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAtt                                                                >  2:492962/1‑150 (MQ=255)
                                                                                       ccgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAGGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTa                                                               >  1:1419566/1‑150 (MQ=255)
                                                                                        cgccCGTCCGCAGCTACTCTCAACACGATAGTTCGTCATCTTCTGCCTCTAAAACAAAGCCTTTGCTATGTTACATGCTAAGCCAAACGAGAAAGATTATCAAATAAAAATAAATGGATTGTGGTGATTTCTAAGATAGACCAGtattat                                                              >  1:421792/1‑150 (MQ=255)
                                                                                         gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                             <  2:529544/150‑1 (MQ=255)
                                                                                          cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGa                                                            >  2:1351532/1‑150 (MQ=255)
                                                                                             gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                         >  2:592750/1‑150 (MQ=255)
                                                                                                   tgctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGaaaa                                                   >  2:1485382/1‑150 (MQ=255)
                                                                                                    gctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAACACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTAATTTAAATGGATTGTGGTGATTTCTCTGATAGACAAGGATTATGACTCCGAAAAg                                                  >  2:362174/1‑150 (MQ=255)
                                                                                                        ctctCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtg                                              >  2:682555/1‑150 (MQ=255)
                                                                                                          ctcAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtgat                                            >  1:334013/1‑150 (MQ=255)
                                                                                                          ctcAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCAATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGAAATCCCAGGATTATGACTACGAAAAGATtgat                                            >  1:1411946/1‑150 (MQ=255)
                                                                                                             aaCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGAc                                         >  2:190811/1‑150 (MQ=255)
                                                                                                             aaCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTATGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGAc                                         >  1:1724293/1‑150 (MQ=255)
                                                                                                              aCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACt                                        <  1:544290/150‑1 (MQ=255)
                                                                                                                  gATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGaa                                    <  2:1535050/150‑1 (MQ=255)
                                                                                                                    tAGTTCGTCATCTTGTGCCTCTAAAAGATAGGCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                  <  2:1701346/150‑1 (MQ=255)
                                                                                                                     aGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgt                                 >  1:328874/1‑150 (MQ=255)
                                                                                                                       ttCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTCTGGTGATTTATCTGATAGACAAGGATTATGACTACGAAAAGATTGATAACTTGAATgtta                               >  2:130074/1‑150 (MQ=255)
                                                                                                                         cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                             >  2:809812/1‑150 (MQ=255)
                                                                                                                           tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggt                           >  1:692824/1‑150 (MQ=255)
                                                                                                                           tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACAAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggt                           >  1:2110467/1‑150 (MQ=255)
                                                                                                                              tCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggttat                        >  1:175553/1‑150 (MQ=255)
                                                                                                                                ttGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGt                      <  1:1845536/150‑1 (MQ=255)
                                                                                                                                  gtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt                    >  2:948073/1‑150 (MQ=255)
                                                                                                                                  gtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt                    <  1:2178200/150‑1 (MQ=255)
                                                                                                                                  gtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt                    >  2:2201395/1‑150 (MQ=255)
                                                                                                                                    gCCTCTAAAACATAGCCTTTTCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                  <  1:124028/150‑1 (MQ=255)
                                                                                                                                     ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                 <  1:1177662/150‑1 (MQ=255)
                                                                                                                                     ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                 <  1:632928/150‑1 (MQ=255)
                                                                                                                                      ctctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg                >  1:1751472/1‑150 (MQ=255)
                                                                                                                                      ctctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAAGGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAtt                >  1:1482187/1‑149 (MQ=255)
                                                                                                                                      acatgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg                >  1:1433795/6‑150 (MQ=255)
                                                                                                                                       catgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt               >  1:1615281/5‑150 (MQ=255)
                                                                                                                                       catgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt               >  1:1036919/5‑150 (MQ=255)
                                                                                                                                       catgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt               >  2:703496/5‑150 (MQ=255)
                                                                                                                                        ctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt              <  2:538551/150‑1 (MQ=255)
                                                                                                                                        ctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt              <  1:654648/150‑1 (MQ=255)
                                                                                                                                           aaaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGc           <  2:654577/150‑1 (MQ=255)
                                                                                                                                                 ggCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACAttt     >  1:1863466/2‑150 (MQ=255)
                                                                                                                                                  gCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTa    <  2:1028776/150‑1 (MQ=255)
                                                                                                                                                   ccTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTAc   >  1:116118/1‑150 (MQ=255)
                                                                                                                                                    cTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACa  >  1:1949906/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACA  >  NC_000913/2512580‑2512877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: