Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,730 T→A 5.5% intergenic (‑24/‑312) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,7300TA 5.5% 400.9 / 9.0 137intergenic (‑24/‑312)mntH/nupCmanganese/divalent cation transporter/nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base T (75/52);  new base A (5/3);  total (82/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 138 total aligned reads displayed.

ATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACA  >  NC_000913/2512582‑2512877
                                                                                                                                                    |                                                                                                                                                   
  atTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGc                                                                                                                                                  <  1:1617263/150‑1 (MQ=255)
   ttGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCCACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCt                                                                                                                                                 >  1:427542/1‑150 (MQ=255)
     ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                               <  1:867797/150‑1 (MQ=255)
     ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                               >  1:575884/1‑150 (MQ=255)
      gTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATg                                                                                                                                              >  2:1417226/1‑150 (MQ=255)
      gTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATg                                                                                                                                              >  1:359632/1‑150 (MQ=255)
       tCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGt                                                                                                                                             >  2:1845536/1‑150 (MQ=255)
        cGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                            >  1:654577/1‑150 (MQ=255)
        cGCAAAGTTACCGGGATAGATATAACCAATCACCGCAATGAACGCAGGGCCCATTAATGCGCGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                            <  2:92594/150‑1 (MQ=255)
          cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                          <  2:230859/150‑2 (MQ=255)
              gTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGc                                                                                                                                      >  2:1825986/1‑150 (MQ=255)
                tACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCGCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCAGCTACTCTCAACGCGATAGTTCGTCATCTTGAGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTa                                                                                                                                    >  1:1421174/1‑150 (MQ=255)
                  actgtaTCGATATAACCAATCGCCGAAATGAACGCAGGAACCATTACTGCGAGCCTCATCTTGCGCGCCGCCCGTCCTGTTATACTCTCAACGCGCTAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATg                                                                                                                                  <  2:1048412/145‑1 (MQ=255)
                    gggATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGcc                                                                                                                                <  1:1636774/150‑1 (MQ=255)
                      gATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCaa                                                                                                                              >  1:92530/1‑150 (MQ=255)
                       aTCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTTCTCTCACCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTTCTATGTGTCATGCTATGCCaaa                                                                                                                             >  2:124028/1‑150 (MQ=255)
                          gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACga                                                                                                                          >  2:857054/1‑150 (MQ=255)
                          gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCAGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTGTGCTATGTTTCATGCTATGCCAAACgc                                                                                                                          >  1:1134226/1‑149 (MQ=255)
                           atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         >  1:2017114/1‑150 (MQ=255)
                           atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         <  1:1253369/150‑1 (MQ=255)
                           atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         >  2:632928/1‑150 (MQ=255)
                           atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCATTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         >  2:1008947/1‑150 (MQ=255)
                             ataACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCTAGCCTCATCTTGCGCGACGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTTAAACATAGCCTTTGCTATGTTTCATGCTATGCCAATCGAGaa                                                                                                                       <  1:1269433/150‑1 (MQ=255)
                                 ccAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGAt                                                                                                                   <  1:23301/150‑1 (MQ=255)
                                  cAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGAtt                                                                                                                  >  1:538551/1‑150 (MQ=255)
                                  cAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCATCATCTTGCGCGACGCCCGTCCGCTGGTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGAtt                                                                                                                  <  1:1420520/150‑1 (MQ=255)
                                   aaTCGCCGCAATGATCGCAGGTGCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATTCTATGCCAAACGAGAATGATTa                                                                                                                 <  1:559926/150‑1 (MQ=255)
                                       gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaa                                                                                                             >  1:2090402/1‑150 (MQ=255)
                                        ccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaaa                                                                                                            >  2:1251564/1‑150 (MQ=255)
                                          gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAtt                                                                                                          >  1:538394/1‑150 (MQ=255)
                                           cAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTc                                                                                                         >  1:1869452/1‑150 (MQ=255)
                                            aaTGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCa                                                                                                        >  1:1912583/1‑150 (MQ=255)
                                              tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                      <  2:1931618/150‑1 (MQ=255)
                                              tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                      >  2:20359/1‑150 (MQ=255)
                                               gatcGCAGGTCCCATTAATGCGAGCCTCATCTTGGGCGCCGCCAGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCTTGCTATGCCAAACGTGTATGTTTTTCAAATTCAttt                                                                                                     <  2:1450291/147‑1 (MQ=255)
                                               gAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAttt                                                                                                     >  2:1177662/1‑150 (MQ=255)
                                               gAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAttt                                                                                                     >  1:153639/1‑150 (MQ=255)
                                               gAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAttt                                                                                                     >  1:448657/1‑150 (MQ=255)
                                                 aCGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTACATGCTATGCCAAACGATAATGATTATCAAATTCCTTTaa                                                                                                   >  2:140938/1‑150 (MQ=255)
                                                   tcAGGTACCATTAATGCGAGCCTTATCTTGCGCTCCGCCCGTCATCTTCTACTCTCAACGCGATAGTTCGTCATCTTGTTCCTCTAAAACATAGCCTTTGCTATGTTTAATGCTATGCCAAACGATAATGATTATCAAATTCATTTAAAt                                                                                                 <  2:80056/149‑1 (MQ=255)
                                                    cAGGTCCCATTAATGCTAGCCTCATCTTGCGCGACGCACGTCCGCTGCTACTCTCACCGCGATAGTTCGGAATATTGTGCCTCTATAACATAGCCTTTGCTATGTTTCATGCTATGGCACACGAGAATGATTTTGATAATCAttatcgtt                                                                                                <  2:541574/150‑7 (MQ=255)
                                                    cAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATg                                                                                                <  2:448657/150‑1 (MQ=255)
                                                      ggTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGa                                                                                              >  1:182621/1‑150 (MQ=255)
                                                         cccATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtg                                                                                           >  1:1028776/1‑150 (MQ=255)
                                                         cccATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtg                                                                                           <  1:2094527/150‑1 (MQ=255)
                                                           cATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtgtg                                                                                         >  1:705111/1‑150 (MQ=255)
                                                            aTTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATATTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtgg                                                                                        <  2:1206601/150‑1 (MQ=255)
                                                             ttAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtgggct                                                                                       <  1:437781/150‑5 (MQ=255)
                                                             ttAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                       <  2:1657664/150‑1 (MQ=255)
                                                             ttAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                       <  2:170400/150‑1 (MQ=255)
                                                               aaTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCGTCTTGTGCCTCTAAAACCTAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCTAATTCATTTAAATGGATTGTGGTGa                                                                                     >  1:369873/1‑150 (MQ=255)
                                                               aaTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGa                                                                                     >  1:961829/1‑150 (MQ=255)
                                                                     aGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATTATTATCAAATTCATTTAAATGGATTGTGGTGATTtctc                                                                               <  1:597955/150‑1 (MQ=255)
                                                                      gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTGTGCTATGTTTCCTGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                              >  1:1335390/1‑150 (MQ=255)
                                                                         tCAGCTTGCGCGCCGCCCGTCCGATGCTACTATCATAGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTggt                                                                           <  1:308829/150‑3 (MQ=255)
                                                                          cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATATTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                          <  2:1912583/150‑1 (MQ=255)
                                                                           aTCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAg                                                                         >  2:1043193/1‑150 (MQ=255)
                                                                           aTCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAg                                                                         >  2:493383/1‑150 (MQ=255)
                                                                             cTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAc                                                                       >  2:231277/1‑150 (MQ=255)
                                                                               tGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCa                                                                     >  1:1795946/1‑150 (MQ=255)
                                                                                  gcggcgCCCGTCCGCTGCTAATCTCATCGCGATATTTCGTCATCTTGTGCCTCTATAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCGAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGa                                                                  <  1:1295933/150‑1 (MQ=255)
                                                                                  gcgcCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGa                                                                  <  2:245998/150‑1 (MQ=255)
                                                                                   cgccgcCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAt                                                                 >  2:1869078/1‑150 (MQ=255)
                                                                                    gccgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAtt                                                                >  2:492962/1‑150 (MQ=255)
                                                                                     ccgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAGGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTa                                                               >  1:1419566/1‑150 (MQ=255)
                                                                                      cgccCGTCCGCAGCTACTCTCAACACGATAGTTCGTCATCTTCTGCCTCTAAAACAAAGCCTTTGCTATGTTACATGCTAAGCCAAACGAGAAAGATTATCAAATAAAAATAAATGGATTGTGGTGATTTCTAAGATAGACCAGtattat                                                              >  1:421792/1‑150 (MQ=255)
                                                                                        cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGa                                                            >  2:1351532/1‑150 (MQ=255)
                                                                                           gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                         >  2:592750/1‑150 (MQ=255)
                                                                                             ccGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACg                                                       >  2:538040/1‑150 (MQ=255)
                                                                                                 tgctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGaaaa                                                   >  2:1485382/1‑150 (MQ=255)
                                                                                                  gctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAACACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTAATTTAAATGGATTGTGGTGATTTCTCTGATAGACAAGGATTATGACTCCGAAAAg                                                  >  2:362174/1‑150 (MQ=255)
                                                                                                     aCTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAtt                                               >  1:742306/1‑150 (MQ=255)
                                                                                                      ctctCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtg                                              >  2:682555/1‑150 (MQ=255)
                                                                                                        ctcAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCAATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGAAATCCCAGGATTATGACTACGAAAAGATtgat                                            >  1:1411946/1‑150 (MQ=255)
                                                                                                         tctaCGCGATTGTTAGTCAATTTGTGACTCTAAAACATAGCATTTGTTATGTTTCATGCTATGCCAAACGAGATTGATTATGAAATTCATTTAAATTGATTGTGGTGATTTCTCTGATAGACCAGGATTATGTCTACGAAAAGATtgata                                           <  1:220439/147‑2 (MQ=255)
                                                                                                         tcAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtgatg                                           <  2:359632/150‑1 (MQ=255)
                                                                                                           aaCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGAc                                         >  2:190811/1‑150 (MQ=255)
                                                                                                           aaCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTATGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGAc                                         >  1:1724293/1‑150 (MQ=255)
                                                                                                                gATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGaa                                    <  2:1535050/150‑1 (MQ=255)
                                                                                                                  tAGTTCGTCATCTTGTGCCTCTAAAAGATAGGCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                  <  2:1701346/150‑1 (MQ=255)
                                                                                                                   aGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgt                                 >  1:328874/1‑150 (MQ=255)
                                                                                                                     ttCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTCTGGTGATTTATCTGATAGACAAGGATTATGACTACGAAAAGATTGATAACTTGAATgtta                               >  2:130074/1‑150 (MQ=255)
                                                                                                                       cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                             >  2:809812/1‑150 (MQ=255)
                                                                                                                         tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACAAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggt                           >  1:2110467/1‑150 (MQ=255)
                                                                                                                           aTCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggtta                         <  1:20359/150‑1 (MQ=255)
                                                                                                                            tCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggttat                        >  1:175553/1‑150 (MQ=255)
                                                                                                                                gtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt                    >  2:948073/1‑150 (MQ=255)
                                                                                                                                gtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt                    <  1:2178200/150‑1 (MQ=255)
                                                                                                                                  gCCTCTAAAACATAGCCTTTTCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                  <  1:124028/150‑1 (MQ=255)
                                                                                                                                   ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                 <  1:1177662/150‑1 (MQ=255)
                                                                                                                                    ctctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg                >  1:1751472/1‑150 (MQ=255)
                                                                                                                                    ctctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAAGGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAtt                >  1:1482187/1‑149 (MQ=255)
                                                                                                                                     catgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt               >  1:1615281/5‑150 (MQ=255)
                                                                                                                                     catgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt               >  2:703496/5‑150 (MQ=255)
                                                                                                                                     catgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt               >  1:1036919/5‑150 (MQ=255)
                                                                                                                                      ctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt              <  1:654648/150‑1 (MQ=255)
                                                                                                                                      ctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt              <  2:538551/150‑1 (MQ=255)
                                                                                                                                         aaaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGc           <  2:654577/150‑1 (MQ=255)
                                                                                                                                               ggCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACAttt     >  1:1863466/2‑150 (MQ=255)
                                                                                                                                                 ccTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTAc   >  1:116118/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACA  >  NC_000913/2512582‑2512877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: