Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,525,907 G→A 6.2% intergenic (+16/+23) yfeN → / ← yfeR putative outer membrane protein/transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9070GA 6.2% 406.4 / 10.2 130intergenic (+16/+23)yfeN/yfeRputative outer membrane protein/transcriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base G (60/61);  new base A (4/4);  total (64/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 134 total aligned reads displayed.

ATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAC  >  NC_000913/2525758‑2526056
                                                                                                                                                     |                                                                                                                                                     
  ggCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCt                                                                                                                                                     >  1:2038867/1‑150 (MQ=255)
   gcgcAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTc                                                                                                                                                    >  1:1915610/1‑150 (MQ=255)
       aGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCgg                                                                                                                                                <  1:2039078/150‑1 (MQ=255)
           gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAga                                                                                                                                            >  2:2215653/1‑149 (MQ=255)
           gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGt                                                                                                                                            <  1:1519597/150‑1 (MQ=255)
           gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGt                                                                                                                                            <  1:184025/150‑1 (MQ=255)
            cACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTa                                                                                                                                           <  1:414520/150‑1 (MQ=255)
            aaCTGTGGTGGAATGCAACATCACACATTACTACGGGGAATCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTa                                                                                                                                           <  1:868669/149‑1 (MQ=255)
              cTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATg                                                                                                                                         >  2:760522/1‑150 (MQ=255)
              cTGAGGGGGAATGCAACATCACACATTACTACGCGGATTCAGTATCGATATGCGGATGACAAACTCGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaat                                                                                                                                         <  1:354957/150‑3 (MQ=255)
                gtggtgGAATGCAACATCACAGATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGaa                                                                                                                                       >  1:2201930/1‑150 (MQ=255)
                  ggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaatcaat                                                                                                                                     <  1:496578/150‑7 (MQ=255)
                   gtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaatcaatc                                                                                                                                    <  1:1194019/150‑8 (MQ=255)
                     ggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCAc                                                                                                                                  <  1:1546110/150‑1 (MQ=255)
                       aaTGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCActc                                                                                                                                <  1:845868/150‑1 (MQ=255)
                          gCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtat                                                                                                                             >  2:1756280/1‑150 (MQ=255)
                           cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtata                                                                                                                            <  2:545904/150‑1 (MQ=255)
                           cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtata                                                                                                                            >  2:1490512/1‑150 (MQ=255)
                           cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtata                                                                                                                            >  2:1486780/1‑150 (MQ=255)
                               aTCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTg                                                                                                                        >  1:1191163/1‑150 (MQ=255)
                                tCACACATTACTACGGTGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGa                                                                                                                       <  1:2089063/150‑1 (MQ=255)
                                tCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCAATCTATATCTGa                                                                                                                       >  2:2004512/1‑150 (MQ=255)
                                 cacacaTTACTACGGGGATTCAGTATCGATATTCGTATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGAt                                                                                                                      <  2:837290/150‑1 (MQ=255)
                                 cacacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGAt                                                                                                                      <  1:974815/150‑1 (MQ=255)
                                 cacacaTTACTACGGGGATTCAGTATCGATATGCGGAAGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGAt                                                                                                                      <  1:641409/150‑1 (MQ=255)
                                  acacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATa                                                                                                                     <  1:742330/150‑1 (MQ=255)
                                  acacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAAATCACTCTATATCTtata                                                                                                                     >  2:310318/1‑150 (MQ=255)
                                      aTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAAc                                                                                                                 <  2:461875/150‑1 (MQ=255)
                                          ctacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATATGATACAACGGAt                                                                                                             >  1:736130/1‑150 (MQ=255)
                                           tacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATc                                                                                                            <  1:1401941/150‑1 (MQ=255)
                                           accGGGTATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATc                                                                                                            <  2:1500276/148‑1 (MQ=255)
                                            acGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATcc                                                                                                           >  1:1702773/1‑150 (MQ=255)
                                             cGGGTATTCAGTATCTATATGCGTATGACAAACTGGGCTAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTTATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATccc                                                                                                          <  1:670283/150‑1 (MQ=255)
                                             cGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATccc                                                                                                          >  1:1438801/1‑150 (MQ=255)
                                             cGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATccc                                                                                                          <  1:2173592/150‑1 (MQ=255)
                                              ggggATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATcccc                                                                                                         <  2:3894/150‑1 (MQ=255)
                                                 gATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                     >  1:875984/1‑150 (MQ=255)
                                                  aTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                     <  2:1748647/150‑1 (MQ=255)
                                                  aTTCAGTATCGACATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                     >  1:2037167/1‑150 (MQ=255)
                                                      aGTATCGATATGCGGATGACAATCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                 <  2:1670963/150‑1 (MQ=255)
                                                       gTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                <  2:1615177/150‑1 (MQ=255)
                                                       gTATCGATATGCGGATGACAAAATTTGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                <  2:1606641/150‑1 (MQ=255)
                                                            gATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCa                                                                                           >  2:1249205/1‑150 (MQ=255)
                                                                tGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg                                                                                       >  2:1607464/1‑150 (MQ=255)
                                                                tGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg                                                                                       <  1:1431998/150‑1 (MQ=255)
                                                                tGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg                                                                                       >  2:819079/1‑150 (MQ=255)
                                                                  cGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg                                                                                     <  2:1039430/150‑1 (MQ=255)
                                                                   ggATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcgc                                                                                    >  1:988623/1‑150 (MQ=255)
                                                                      tGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAtt                                                                                 <  1:1317558/150‑1 (MQ=255)
                                                                       taCAAACTGGGCGAAGATTTTTTCCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGTGGTCCGGGCAGTATGAATTCACTCTATATCTGATACAAGGGATCCCGTTCCCGCCCAGCCATTAGCGCATTg                                                                                <  2:1681394/149‑1 (MQ=255)
                                                                       gACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATACCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTg                                                                                <  1:1486780/150‑1 (MQ=255)
                                                                       gACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCAGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTg                                                                                <  2:1915610/150‑1 (MQ=255)
                                                                       gACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATAACTGCCGGGGCTCCCGGCAGTAAGAATTCACTCTTTATCTGATACAACCGATCCCCTTCCCTCACAGCCATTAGCGCAGTg                                                                                >  1:2173783/1‑150 (MQ=255)
                                                                                  cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                     >  2:270450/1‑150 (MQ=255)
                                                                                  cGAAGATTTTTACCAGGAGCCGATAATCTACTCCATCAAATTTAACTTCTGATTGATAACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCACTCCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                     <  2:902221/150‑1 (MQ=255)
                                                                                   gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCACTGTTCTCGt                                                                    >  2:1424962/1‑150 (MQ=255)
                                                                                    aaGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTa                                                                   <  1:1042664/150‑1 (MQ=255)
                                                                                        tttttACCAGGATGCGATAATCTACTCCATCAAATTTAAGTTCTGATTGATTACTGCCGGGGCTCACGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc                                                               >  1:390910/1‑150 (MQ=255)
                                                                                        tttttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc                                                               >  2:479103/1‑150 (MQ=255)
                                                                                                 ggATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaa                                                      <  2:2116354/150‑1 (MQ=255)
                                                                                                   aTGCGTTAATCTACTCCAGCAAATTTTACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCTTTGCCCTGGTCTCGTACCACGTCCCATAAAg                                                    <  1:1995517/150‑1 (MQ=255)
                                                                                                   aTGCGATAAACTACTCCATCAAATTTAACTTCTGATTGAATACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCATCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAg                                                    <  1:1397405/150‑1 (MQ=255)
                                                                                                    tGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGc                                                   >  2:2127059/1‑150 (MQ=255)
                                                                                                    tGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGc                                                   >  1:1834472/1‑150 (MQ=255)
                                                                                                       gATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTc                                                <  1:1364408/150‑1 (MQ=255)
                                                                                                        aTAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCa                                               <  2:736130/150‑1 (MQ=255)
                                                                                                        aTAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCa                                               >  1:1697092/1‑150 (MQ=255)
                                                                                                              tACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg                                         >  1:1008515/1‑150 (MQ=255)
                                                                                                               aCTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGcggcgg                                        >  1:1989661/1‑150 (MQ=255)
                                                                                                               aCTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGcggcgg                                        <  1:1756280/150‑1 (MQ=255)
                                                                                                                 tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                                      <  2:2124783/150‑1 (MQ=255)
                                                                                                                 tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                                      >  1:978204/1‑150 (MQ=255)
                                                                                                                    aTCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaa                                   >  2:294928/1‑150 (MQ=255)
                                                                                                                    aTCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCATGGTCTCGTACAACGTCCCATAAAGCTTCAGCGGAGGTGGaa                                   >  1:1507341/1‑150 (MQ=255)
                                                                                                                      cAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAg                                 >  2:529243/1‑150 (MQ=255)
                                                                                                                        aaTTTAACTTCTGATTGATTACTGCCGGGGATCCCGGCAGTATTAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCACAGCCATTAGCGCATTGCCCTGGTCTCGTACCAAGTCCCATAAAGCTTCAGAGGCGGTGGAAAGTg                               >  2:612224/1‑150 (MQ=255)
                                                                                                                         aTTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                              <  1:1895356/150‑1 (MQ=255)
                                                                                                                          tttAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGATAGTGaa                             >  1:222404/1‑150 (MQ=255)
                                                                                                                          tttAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                             >  1:592618/1‑150 (MQ=255)
                                                                                                                           ttAACTTCTGATTGATTTCTGCCGGGGCTCCCGGCAGTATGAAATCACTCTATATCTGATACAACGGATACCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGTTGGAAAGTGAAc                            <  1:140935/150‑1 (MQ=255)
                                                                                                                           ttAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                            <  1:209626/150‑1 (MQ=255)
                                                                                                                                ttCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGATCGGttc                       >  1:25193/1‑150 (MQ=255)
                                                                                                                                ttCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttc                       >  1:966099/1‑150 (MQ=255)
                                                                                                                                 tCTTATTGATTACTGCCGGGGCTCCCTGCAGTATGAATGCACTCTATATCTGATACAACGGATCCCCTTCCCGCTCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttct                      <  1:1527244/150‑1 (MQ=255)
                                                                                                                                      ttgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg                 <  2:132260/150‑1 (MQ=255)
                                                                                                                                        tcatACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCAGAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg               >  1:1359083/4‑150 (MQ=255)
                                                                                                                                           tACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGAACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAc            <  2:2201930/150‑1 (MQ=255)
                                                                                                                                            aCTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCTTAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAcc           >  1:1410553/1‑150 (MQ=255)
                                                                                                                                            aCTGCCGGGAGTCCCGGCAGTATGAATCCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAcc           >  2:756126/1‑150 (MQ=255)
                                                                                                                                            aCTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAcc           >  1:1606927/1‑150 (MQ=255)
                                                                                                                                             cTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCGTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAccc          >  2:1057326/1‑149 (MQ=255)
                                                                                                                                             cTGCCGGGGCTCCCGGCAGAATGAATTCACTCTATATCTGATACAACGGATCCCCTTACCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTACTGCGCACCa          <  2:926028/150‑1 (MQ=255)
                                                                                                                                             cTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa          >  2:1977049/1‑150 (MQ=255)
                                                                                                                                              tGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg         <  1:2095975/150‑1 (MQ=255)
                                                                                                                                               gCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGGACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGCACGGTTCTTCCTGCGCACCAGc        >  1:1435823/1‑150 (MQ=255)
                                                                                                                                                ccGGGGCTCCCGGCAGTTTGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGACCTGGTCTCGTACCACGTCCCATAAAGCATCAGCGGCCGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCa       >  2:835138/1‑150 (MQ=255)
                                                                                                                                                ccGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTGCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCa       >  2:1597983/1‑150 (MQ=255)
                                                                                                                                                     gCTCCCGGCAGTATGAATTCACTCTATATCTGATACCTCGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc  <  1:1122125/150‑1 (MQ=255)
                                                                                                                                                     gCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc  <  2:2010011/150‑1 (MQ=255)
                                                                                                                                                     gCTCACGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGAGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCTGAGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc  >  2:479541/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAC  >  NC_000913/2525758‑2526056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: