Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,555,201 T→C 5.6% intergenic (‑19/+27) eutR ← / ← eutK eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,555,2010TC 5.6% 362.0 / 7.7 127intergenic (‑19/+27)eutR/eutKeut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base T (60/56);  new base C (4/3);  total (64/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 133 total aligned reads displayed.

GCATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGC  >  NC_000913/2555052‑2555350
                                                                                                                                                     |                                                                                                                                                     
gCATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGtgtt                                                                                                                                                       >  2:1479816/1‑150 (MQ=255)
   tGTTCATAGACTTCCGTTGTGTGTCGTTGATGAACATTATGCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTATGGGTGCATGATAAATATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTa                                                                                                                                                    <  1:419643/150‑1 (MQ=255)
     ttCATAGCCTTCCGTTGTCTGTCGTGTATGAACATTATCCACTTCGACGATCGGGGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGCTAATGTTGGGGCATATTGGCTGTCCGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                  <  1:1171103/150‑1 (MQ=255)
     ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                  <  2:965882/150‑1 (MQ=255)
       cATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                                >  2:1007256/1‑150 (MQ=255)
         tAGCCATCCGTTGTCTGTCTTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTGCAGGTTTTCGGGTAAGGTGTCATGATTACTATGGTGCATATTGGCTGTACGGGTCTTTGTCATGATGTAAATGCATGGTGTTGTAGGacac                                                                                                                                              <  2:1932142/150‑1 (MQ=255)
         tAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGacac                                                                                                                                              >  1:651877/1‑150 (MQ=255)
         tAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGacac                                                                                                                                              <  2:1865488/150‑1 (MQ=255)
         tAGACATCCGTCGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGacac                                                                                                                                              <  2:1228689/150‑1 (MQ=255)
          aGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACAccc                                                                                                                                            >  2:973161/1‑150 (MQ=255)
           gACTTCAGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACAccc                                                                                                                                            <  1:324212/150‑1 (MQ=255)
              aTCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGc                                                                                                                                         >  1:1239366/1‑150 (MQ=255)
              aTCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGc                                                                                                                                         <  1:522268/150‑1 (MQ=255)
                ccGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTTAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                  <  1:309702/139‑1 (MQ=255)
                ccGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                  >  2:309702/1‑139 (MQ=255)
                ccGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCGGCat                                                                                                                                       <  1:2031681/150‑2 (MQ=255)
                 cGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGCAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                      <  1:720668/150‑1 (MQ=255)
                        tgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACa                                                                                                                               >  1:1286054/1‑150 (MQ=255)
                            gTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTGATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGtt                                                                                                                           >  2:1573098/1‑150 (MQ=255)
                               gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                        >  2:1004983/1‑150 (MQ=255)
                                 tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                      >  2:1797501/1‑150 (MQ=255)
                                 tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                      >  2:251850/1‑150 (MQ=255)
                                 tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                      >  2:377580/1‑150 (MQ=255)
                                 tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGAGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                      >  2:427884/1‑150 (MQ=255)
                                  gaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATTCCGGGTGTTGTAGGACACACGGCACCTCCGACAGGTTAATggg                                                                                                                     >  2:472979/1‑150 (MQ=255)
                                  gaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATggg                                                                                                                     >  2:859933/1‑150 (MQ=255)
                                    aCATTATCCACTTCGCCCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                   <  1:1133321/150‑1 (MQ=255)
                                       ttATCCACTTCGACCTTCGGCGTGAGCTTCTGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                <  2:588139/150‑1 (MQ=255)
                                         aTCCACTTCGACCTTCGGCGTGAGCTTCAGGGTTTCGGGTAAGGGGTGATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTgag                                                                                                              <  2:1177949/150‑1 (MQ=255)
                                          tCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTgaga                                                                                                             >  2:1358737/1‑150 (MQ=255)
                                                tCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATaa                                                                                                       >  1:1062304/1‑150 (MQ=255)
                                                  gACCTTCGGCGTGAGCTTCAGGTTTTGGGGTAAGGGTTCATGATAAAGATGGTGAAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACg                                                                                                     <  2:137484/150‑1 (MQ=255)
                                                    ccTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATTATGTTAATGCGGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGac                                                                                                   <  2:733412/150‑1 (MQ=255)
                                                     gttgggCGTGAGATTCAGTTTTTCGGGTATGGGTTCATTATAAAGATGGTGAAATTTGGATGTACGGGTCTTTTTCATGTTGTTATTGCGGGGTGTTGTTGGAAACCGGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGact                                                                                                  <  2:1766441/146‑1 (MQ=255)
                                                     cTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGact                                                                                                  >  1:629522/1‑150 (MQ=255)
                                                        gggCGGGAGCTTCAGGTTTTCGGGTATGGGTGCATGATAAAGATGGTGCAAAGTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTGGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGactact                                                                                               <  2:313512/149‑1 (MQ=255)
                                                            gTGAGCTTCAGGTTTTCGGGTAAGGGGTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGt                                                                                           <  1:423138/150‑1 (MQ=255)
                                                             tGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGtt                                                                                          <  1:1535985/150‑1 (MQ=255)
                                                               aGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTa                                                                                        >  2:1564363/1‑150 (MQ=255)
                                                                 cTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACg                                                                                      >  1:174091/1‑150 (MQ=255)
                                                                 cTTCAGGTTTTCGGGTAAGGGTTAATTATAAAGTTGGTGCAATTTGGCTGTACGGGTCTTTTTCATGATGTTATTGCCGGGTGTTGTAGTACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACg                                                                                      <  1:2074255/150‑1 (MQ=255)
                                                                   tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                    <  1:1463216/150‑1 (MQ=255)
                                                                   tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                    >  1:1015236/1‑150 (MQ=255)
                                                                    ctgttgttCGGTTAAGGGTTCATGATAATGATGGGGCAAATTGGCTGTACGGTTCTTTTTCTTGAGGTTAAGGCGGGGTGTTGTAGGACACCGGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTaa                                                                                   <  1:1619363/144‑1 (MQ=255)
                                                                    cAGGTTTTCGGGTAAGCGTTCATGATAAAGCTGGTGCAATTTGGCTGTCCGGGGCTTTTGCATGATGTTAATGCCGGGGGTTGTTGGACACCCGGCACCTCCGACAGGTTAATGCGGCTTGAGACGATAACGACTACTGCGTTTACGTaa                                                                                   <  1:354890/150‑1 (MQ=255)
                                                                     aGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTGGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAAc                                                                                  <  2:1468084/150‑1 (MQ=255)
                                                                         tttCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTcc                                                                              >  2:1169514/1‑150 (MQ=255)
                                                                         tttCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTcc                                                                              >  2:1640313/1‑150 (MQ=255)
                                                                           tCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCg                                                                            <  2:1142983/150‑1 (MQ=255)
                                                                           tCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCg                                                                            <  2:1150691/150‑1 (MQ=255)
                                                                             gggTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGc                                                                          <  1:837318/150‑1 (MQ=255)
                                                                               ttatgggTTCATTAAAAAGATGTTGAAAATTGGCTGTACGGGTCTTTTTCTTTATGTTAATGCGGTGTGTTGTTGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAg                                                                        <  2:1612129/146‑1 (MQ=255)
                                                                                 aaGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGaa                                                                      <  2:651877/150‑1 (MQ=255)
                                                                                  aGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCTGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGaaa                                                                     >  1:556585/1‑150 (MQ=255)
                                                                                  aGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGCCTACTGCGTTTACGTAACGTCCCGGCAGaaa                                                                     >  1:1500415/1‑150 (MQ=255)
                                                                                    ggTTCATGATAAAGATGGTGCAAATTGGCTGTACGTGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAag                                                                   <  1:427884/150‑1 (MQ=255)
                                                                                    ggTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAag                                                                   <  2:398156/150‑1 (MQ=255)
                                                                                        cATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTCGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCt                                                               >  1:1800070/1‑150 (MQ=255)
                                                                                         aTGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTg                                                              >  2:617094/1‑150 (MQ=255)
                                                                                         aTGATAAAGAGGGTGCAAATTGGCGGTACGGGGCTTTTTCATGATGTTAATGCCGGGGGGGGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTg                                                              <  2:1943082/150‑1 (MQ=255)
                                                                                          tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGCCTACTGCGTTTACGTAACGTCCCGGCAGAAAAGCGCTGt                                                             >  2:1106102/1‑150 (MQ=255)
                                                                                          tGATAAAGATGGTGCAAATTGGCTGGACGGGTCGTTTTCATGATGTTAATGCCGGGTGGTGGAGGCCACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTCTACGTAACGGCCCGGCAGAAAAGAGCTGt                                                             <  2:370504/150‑1 (MQ=255)
                                                                                           gATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGtt                                                            >  1:1019682/1‑150 (MQ=255)
                                                                                             tAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCg                                                          <  2:2107267/150‑1 (MQ=255)
                                                                                               aaGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                        <  1:1573098/150‑1 (MQ=255)
                                                                                                aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                       >  2:2009466/1‑150 (MQ=255)
                                                                                                 tagggTGCAAATGGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgcg                                                      <  1:1958677/147‑1 (MQ=255)
                                                                                                       gCAACTTGGCTCTACGGGGCTTTTTCATAATGGTAATGCCCGGTGTCGTAGGACACCCTGCACCTCGGAGAGGTTAATGTGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTc                                                <  1:180810/150‑1 (MQ=255)
                                                                                                        cAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCt                                               >  1:1114451/1‑150 (MQ=255)
                                                                                                         aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACGGCGTTTACGTAACGGCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                              >  2:1869756/1‑150 (MQ=255)
                                                                                                           aTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                            <  2:1239366/150‑1 (MQ=255)
                                                                                                            ttGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCt                                           <  2:174091/150‑1 (MQ=255)
                                                                                                              ggCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCCCCTCCGACAGGTTAATGGGGCTTGGGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAATAGCTGTTCGAGCGCATTTCTGGCttt                                         >  1:1131402/1‑150 (MQ=255)
                                                                                                                 tGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGGTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTc                                      <  2:1138682/150‑1 (MQ=255)
                                                                                                                   tACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCa                                    >  1:1296046/1‑150 (MQ=255)
                                                                                                                        gTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgc                               >  1:996830/1‑150 (MQ=255)
                                                                                                                          cTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGCGCGCATTTCTGGCTTTTTCCAGCGgcca                             >  1:2003444/1‑150 (MQ=255)
                                                                                                                           ttattCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccag                            <  1:1479816/147‑1 (MQ=255)
                                                                                                                                cATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaaa                       >  1:1356406/1‑150 (MQ=255)
                                                                                                                                cATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGAAGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaaa                       >  2:938148/1‑150 (MQ=255)
                                                                                                                                 atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAg                      >  2:1484375/1‑150 (MQ=255)
                                                                                                                                 atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGAGATTACGAAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAg                      >  1:317750/1‑150 (MQ=255)
                                                                                                                                   gatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTg                    >  1:769725/1‑150 (MQ=255)
                                                                                                                                     tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg                  <  1:337656/150‑1 (MQ=255)
                                                                                                                                      gTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc                 <  1:53069/150‑1 (MQ=255)
                                                                                                                                      gTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc                 >  1:21896/1‑150 (MQ=255)
                                                                                                                                        tAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGCCTACTGCGTTTACGTTACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGccg               >  2:836147/1‑148 (MQ=255)
                                                                                                                                        tAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAg               <  1:859933/150‑1 (MQ=255)
                                                                                                                                        tAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTAGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAg               >  2:183156/1‑150 (MQ=255)
                                                                                                                                          ttGCCGGGTGTTGTCGTAAACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc             <  2:1981953/149‑1 (MQ=255)
                                                                                                                                          aTGCCGGGTGTTGTAGGACCCCCGGCACCTCCGACCGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc             <  2:235244/150‑1 (MQ=255)
                                                                                                                                          aTGCCGGGGGGAGTAGGCCACCCGGCACCTCCGACAGGTTAATGCGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc             <  1:227761/150‑1 (MQ=255)
                                                                                                                                           tGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGACCCGGCAGAAAAGAGATGTTCGAGCGCATTTCTCGCTTTTTCAAGCGGCCAGCCAAAGAGGGCAGCCa            >  1:285203/1‑150 (MQ=255)
                                                                                                                                            gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGGAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc           <  1:338980/150‑1 (MQ=255)
                                                                                                                                            gCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc           >  1:1630563/1‑150 (MQ=255)
                                                                                                                                            gCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc           >  2:1273881/1‑150 (MQ=255)
                                                                                                                                             ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt          >  1:1505569/1‑150 (MQ=255)
                                                                                                                                              cGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACtt         >  1:1961703/1‑150 (MQ=255)
                                                                                                                                                  tgttgtAGGACACCCGGCACCTCCGACACGTTAATGGGGCTTGAGACGATACCGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTATTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTcc     <  2:447030/150‑1 (MQ=255)
                                                                                                                                                     tgtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGCAAAGAGCTGGTCGAGCGCATTTCTGGCTTTTTACAGCGGCCCGCCAAAGTGGGCCGCCCCTTCTCCTGc  >  1:1936903/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGC  >  NC_000913/2555052‑2555350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: