Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 209,604 T→C 7.2% intergenic (+24/‑75) accA → / → ldcC acetyl‑CoA carboxylase, carboxytransferase, alpha subunit/lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913209,6040TC 7.2% 283.4 / 11.6 113intergenic (+24/‑75)accA/ldcCacetyl‑CoA carboxylase, carboxytransferase, alpha subunit/lysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base T (47/53);  new base C (4/4);  total (51/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

Only 100 of 115 total aligned reads displayed.

CCGGAAGCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGC  >  NC_000913/209455‑209752
                                                                                                                                                     |                                                                                                                                                    
ccGGAAGCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGACTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAAGGt                                                                                                                                                      >  1:1612640/1‑150 (MQ=255)
  ggAAGCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGGCTGATGCGCTACGGTGACGCGTAATTCGCAAATGTTCTGAAAAGGGGtgg                                                                                                                                                    >  2:2204485/1‑148 (MQ=255)
       cGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCg                                                                                                                                               >  2:2137290/1‑150 (MQ=255)
           ggcggcATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTgg                                                                                                                                           <  1:1735201/150‑1 (MQ=255)
           ggcggcATCGTTGAAAGCGAAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTGCTGAAAAAGGGTCACTTCGGTgg                                                                                                                                           <  2:514593/150‑1 (MQ=255)
            gcggcATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGc                                                                                                                                          >  1:1549006/1‑150 (MQ=255)
                cATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtt                                                                                                                                      >  2:761866/1‑150 (MQ=255)
                  tCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtttt                                                                                                                                    <  2:138978/150‑1 (MQ=255)
                   cGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCCGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTACTGAAAAAGGGTCACTTCGGTGGCCCttttt                                                                                                                                   >  1:1522372/1‑150 (MQ=255)
                    gTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtttttt                                                                                                                                  >  2:1930770/1‑150 (MQ=255)
                     ttGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGCCACCGAAGTGACCCtttttca                                                                                                                                 <  1:535184/150‑3 (MQ=255)
                      tGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGCCACCGAAGTGACCCtttttcag                                                                                                                                <  1:1694727/150‑4 (MQ=255)
                       gAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCATCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATc                                                                                                                               >  2:345707/1‑150 (MQ=255)
                        aaaGCGCAACTGCTTGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGCCACCGAAGTGACCCtttttcagaa                                                                                                                              <  1:321703/150‑6 (MQ=255)
                         tagagcAACTGGTGGCGGTTCTGGCAGATGACGACGTGTTAAGCACTGATTATTTAAAATATCGTAGTTTTCAGCGCCTGATGAGCTACGGTAACGCGCAATTAGCAATAGTTCTGAAAAAGGGTCACTTAGGTTGCCCTTTTTTATCGc                                                                                                                             <  2:408722/146‑1 (MQ=255)
                            cgcAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGCTGCGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTGTTTTTCGCCAc                                                                                                                          >  2:1779828/1‑150 (MQ=255)
                               aaCTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGt                                                                                                                       <  2:706050/150‑1 (MQ=255)
                                 ctgctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGttt                                                                                                                     <  2:892863/150‑1 (MQ=255)
                                  tgctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGTGCTACGGATACGCGTAATTCGCAAAAGTTCTGAAAAAGGGGCACTTCGGTGGCCCTTTTTTATCGCCTCGGTTTg                                                                                                                    <  1:1863422/150‑1 (MQ=255)
                                   gctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGa                                                                                                                   <  2:1456216/150‑1 (MQ=255)
                                   gctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGa                                                                                                                   >  2:406173/1‑150 (MQ=255)
                                    cttccGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCTTAATTCGCAAAAGTTCTGAAAAAGTGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                                                  <  1:1677109/146‑1 (MQ=255)
                                    ctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                                                  <  2:320072/150‑1 (MQ=255)
                                    ctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCGTTTTTATCGCCACGGTTTGAg                                                                                                                  >  1:1396700/1‑150 (MQ=255)
                                      ggCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCa                                                                                                                >  2:927964/1‑150 (MQ=255)
                                      ggCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGACCTTTTTTATCGCGGCGGTTTGAGCa                                                                                                                >  2:313528/1‑150 (MQ=255)
                                        cGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTATCGCCACGGTTTGAGCAGGc                                                                                                             >  2:1346272/1‑150 (MQ=255)
                                         ggATCTGGCCGATCTCGACGTGTTAAGCAATGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGc                                                                                                             <  2:1217764/150‑1 (MQ=255)
                                           aTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTa                                                                                                           <  1:2041994/150‑1 (MQ=255)
                                           aTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTa                                                                                                           <  1:391238/150‑1 (MQ=255)
                                            tCTGGCCGTTCTCGACGTGTTCAGCCCTGACGAATTAAAAAATCGTCGTTATCAGAGCCTGATGAGCTACGGGTACGCGTAATTCGAAAAAGTTCTGAAAAAGGGTCACATCGGTGGCCCTTTTTTATCTCCACGGTTTGAGCAGGCTAt                                                                                                          <  1:1155582/150‑1 (MQ=255)
                                             cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATg                                                                                                         >  2:2046818/1‑150 (MQ=255)
                                             cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATg                                                                                                         <  2:1166583/150‑1 (MQ=255)
                                              tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAATGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGa                                                                                                        <  1:1268792/150‑1 (MQ=255)
                                               ggCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGAt                                                                                                       >  1:609035/1‑150 (MQ=255)
                                                gCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGAtt                                                                                                      >  1:1323323/1‑150 (MQ=255)
                                                 ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                     >  2:1854195/1‑150 (MQ=255)
                                                 ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                     <  2:1555007/150‑1 (MQ=255)
                                                 ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                     >  2:645816/1‑150 (MQ=255)
                                                   gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaag                                                                                                   <  2:1491260/150‑1 (MQ=255)
                                                     tctcGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaagga                                                                                                 <  2:1047444/150‑1 (MQ=255)
                                                          aCGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGAt                                                                                            <  2:1977886/150‑1 (MQ=255)
                                                                 aaGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCTAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTGTGAGCATGCTATGATTAAGGAAGGATTTTCCag                                                                                     <  1:1525719/150‑1 (MQ=255)
                                                                 aaGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCag                                                                                     >  1:1229820/1‑150 (MQ=255)
                                                                    cACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggag                                                                                  <  2:246176/150‑1 (MQ=255)
                                                                    cACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggag                                                                                  <  1:1767783/150‑1 (MQ=255)
                                                                      cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCCCGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAggagga                                                                                >  2:1185723/1‑150 (MQ=255)
                                                                      cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGAAAAAGTTCTGAAAAAGGGTCACTTCGTTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTACAggagga                                                                                <  1:1562812/150‑1 (MQ=255)
                                                                      cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCCGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAGGATTTTCCAGGAGGaa                                                                               >  1:828423/1‑150 (MQ=255)
                                                                        gAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAac                                                                              >  1:2124006/1‑150 (MQ=255)
                                                                        gAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGCCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCAAGGAGGAac                                                                              >  2:1226681/1‑150 (MQ=255)
                                                                          atttttAAAAAATGGTCGTTATCAGGGCCTTTAGATCTACGGTTTCGCGTAATTGGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAacac                                                                            <  1:475890/147‑1 (MQ=255)
                                                                          aGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAacac                                                                            <  1:2205712/150‑1 (MQ=255)
                                                                           gATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAAcaca                                                                           >  2:1770965/1‑150 (MQ=255)
                                                                             tttAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCGTTTTTATCGCCACGGTTTGAGCAGGCTATGAGTAAGGAAGGATTTTCCAGGAGGAACACATg                                                                         >  2:1065575/1‑150 (MQ=255)
                                                                                aaaaaaTCGTCGGTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAc                                                                      <  2:1205146/150‑1 (MQ=255)
                                                                                  aaaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcat                                                                    >  2:634804/1‑150 (MQ=255)
                                                                                   aaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcatc                                                                   >  1:489474/1‑150 (MQ=255)
                                                                                   aaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcata                                                                   >  1:1163685/1‑149 (MQ=255)
                                                                                     aTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcatcat                                                                 <  2:1159562/150‑1 (MQ=255)
                                                                                        gtcgtTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGCAAACATGCACATCATTGc                                                              >  1:313400/1‑150 (MQ=255)
                                                                                           gtTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTAGGATTAAGGAAGGATTTTCCTGGAGGAACACATGAACATCATTGCCAt                                                           >  1:153127/1‑150 (MQ=255)
                                                                                             tATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTa                                                         <  1:2147516/150‑1 (MQ=255)
                                                                                              ttCAGCGCCTGATGAGCTACGGTTACTCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTAt                                                        <  1:630572/149‑1 (MQ=255)
                                                                                               tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATg                                                       <  2:1024825/150‑1 (MQ=255)
                                                                                               tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATg                                                       <  2:1879419/150‑1 (MQ=255)
                                                                                               tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGACCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATg                                                       <  2:105061/150‑1 (MQ=255)
                                                                                                     ccTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCg                                                 >  2:913218/1‑150 (MQ=255)
                                                                                                      cTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTAGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGc                                                <  2:1613776/150‑1 (MQ=255)
                                                                                                       tgatgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCa                                               <  1:2137290/150‑1 (MQ=255)
                                                                                                         atgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGTGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATg                                             >  2:1199699/1‑150 (MQ=255)
                                                                                                            aGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCg                                          <  2:1142950/150‑1 (MQ=255)
                                                                                                            aGCTAAGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCg                                          <  2:180352/150‑1 (MQ=255)
                                                                                                               tACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCt                                       >  2:1724571/1‑150 (MQ=255)
                                                                                                                 cGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCATTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                     <  2:2068587/150‑1 (MQ=255)
                                                                                                                 cGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                     >  2:263733/1‑150 (MQ=255)
                                                                                                                 aggTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCATGCTATGATTAAGGAATGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                     <  2:1050591/149‑1 (MQ=255)
                                                                                                                   gTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTta                                   <  1:2183296/150‑1 (MQ=255)
                                                                                                                    ttACGCGTAATTCCGAAAAGTTCTGAAAAAGGGGCACTTCGGTGGGCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtat                                  <  2:164999/150‑1 (MQ=255)
                                                                                                                           tAATTCGCAAAAGTTCTGTAAAAGGGGCACATCGGTGGCCCTTTTTTAACGCCACCGTTTGAGCAGGGTATGATTAAGGAAGGATTTTCCAGGACGAACACATGAACATCTTTTCCATTATGTGACCGCATGGCGTCTTTTATAAAGATg                           <  1:345707/150‑1 (MQ=255)
                                                                                                                               tCGCAAAAGTTCTGAAAAACGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGGCTTTTATAAAGATGAGcc                       <  1:1833984/150‑1 (MQ=255)
                                                                                                                                ggCAAAAGTTCTGAATACGGGGCACTTCGGGGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAATGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                      <  2:318703/149‑1 (MQ=255)
                                                                                                                                cGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                      >  2:591776/1‑150 (MQ=255)
                                                                                                                                cGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                      >  1:1039409/1‑150 (MQ=255)
                                                                                                                                 gctaaaGTCCTGAAAACGCGGCACGTCGGTGTCCAGTTTTTTTAGACACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGCAACACATGAACATCATTGCCATAATGGTACCGCATGGCGTCTTTTATAAAGATGAGCCCa                     <  1:444848/147‑1 (MQ=255)
                                                                                                                                 gCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCa                     >  2:1679655/1‑150 (MQ=255)
                                                                                                                                 gCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCa                     >  1:265779/1‑150 (MQ=255)
                                                                                                                                    aaaGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTATCGCCACGGTTTGCGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCaa                 <  2:2174494/150‑1 (MQ=255)
                                                                                                                                      aGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCaaa                >  1:1441110/1‑150 (MQ=255)
                                                                                                                                       gTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTACGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAg               <  1:1451132/150‑1 (MQ=255)
                                                                                                                                        cgataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGa              >  2:1871217/6‑150 (MQ=255)
                                                                                                                                         gataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACAGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGaa             >  2:525215/5‑150 (MQ=255)
                                                                                                                                          cTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCTCAACGGTTTGAGCAGGCTATTATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAAc            <  1:2132430/150‑1 (MQ=255)
                                                                                                                                          ataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCCCGGTTTGAGCAGGCTATGAATAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGCGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAaacaac            >  2:1381635/4‑150 (MQ=255)
                                                                                                                                          ataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCATGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAAc            >  2:1511338/4‑150 (MQ=255)
                                                                                                                                             aaaaaGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTgg         <  2:793244/150‑1 (MQ=255)
                                                                                                                                              aaaaGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGa        >  2:529348/1‑150 (MQ=255)
                                                                                                                                                 aGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTc     >  2:180826/1‑150 (MQ=255)
                                                                                                                                                 aGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTc     <  2:743689/150‑1 (MQ=255)
                                                                                                                                                    gTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGgc  >  1:168413/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGGAAGCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGC  >  NC_000913/209455‑209752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: