Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,638,584 A→T 5.6% F23L (TTT→TTA)  bamB ← BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,638,5840AT 5.6% 423.7 / 9.9 149F23L (TTT→TTA) bamBBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (65/75);  new base T (3/6);  total (68/81)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

Only 100 of 155 total aligned reads displayed.

AGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGC  >  NC_000913/2638436‑2638733
                                                                                                                                                    |                                                                                                                                                     
aGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTaa                                                                                                                                                      >  1:1635736/1‑150 (MQ=255)
 gACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTaaa                                                                                                                                                     >  1:789000/1‑150 (MQ=255)
   cAACGTTGTCCGCCAGTGCCGGATGAAGAATGGAATACAAGTTGACAATGCCAATACCAACGTAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAAATATTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAcaca                                                                                                                                                   >  2:296044/1‑150 (MQ=255)
       gTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGa                                                                                                                                               <  2:1821712/150‑1 (MQ=255)
          gTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaaga                                                                                                                                            <  1:1068540/150‑3 (MQ=255)
          gTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACa                                                                                                                                            <  1:943194/150‑1 (MQ=255)
              gCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGTCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCg                                                                                                                                        >  1:98526/1‑150 (MQ=255)
                cAGTGCCGGATGAAGATTGGAATAGAAGTTGCAAATGCCGCTACCAACGGAAGTGCTCCACGCCGGGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCAAATCTTCTTCGCTGTTAAACAGCGAACAGCCGCt                                                                                                                                      <  1:124512/150‑1 (MQ=255)
                 aGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGTCGCtt                                                                                                                                     <  1:526090/150‑1 (MQ=255)
                          tGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTa                                                                                                                            >  1:813866/1‑150 (MQ=255)
                             aGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACg                                                                                                                         >  1:1562503/1‑150 (MQ=255)
                              gATTGGAATAGAAGTTGCCAATGCCGCTACCCACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACgg                                                                                                                        <  2:602205/150‑1 (MQ=255)
                              gATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACgg                                                                                                                        <  1:1585808/150‑1 (MQ=255)
                               aTTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGa                                                                                                                       <  1:135108/150‑1 (MQ=255)
                                  ggAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCTATGGGGACATCTTTACCCCATCTTCTTCGCTGTTAAACAGCGCACAGCCGCTTAAAAGGGTAACGGAAAg                                                                                                                    >  1:754893/1‑150 (MQ=255)
                                  ggAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACTGAAAg                                                                                                                    >  1:541828/1‑150 (MQ=255)
                                   gAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGc                                                                                                                   <  1:1658108/150‑1 (MQ=255)
                                   gAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGc                                                                                                                   <  1:1144613/150‑1 (MQ=255)
                                     aTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACTCCGTGGTCGGCGTAAACTGGTTTTCAAAGGTTGGCTATGGTGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAg                                                                                                                 <  1:116327/150‑1 (MQ=255)
                                     aTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCg                                                                                                                                                >  1:1017535/1‑119 (MQ=255)
                                     aTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCg                                                                                                                                                <  2:1017535/119‑1 (MQ=255)
                                     aTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAg                                                                                                                 <  2:1355397/150‑1 (MQ=255)
                                       aGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTc                                                                                                               >  2:2099107/1‑150 (MQ=255)
                                          aGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTg                                                                                                            >  1:365537/1‑150 (MQ=255)
                                            ttGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgc                                                                                                          <  1:457902/150‑1 (MQ=255)
                                               ccAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgcagc                                                                                                       <  1:1536866/150‑1 (MQ=255)
                                                cAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgcagca                                                                                                      >  2:1610234/1‑150 (MQ=255)
                                                cAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgcagca                                                                                                      >  2:760565/1‑150 (MQ=255)
                                                    gCCGCTAACAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGCCAATGGGGACATCTTTACCACCTCTTCTTCGATGTTAAACAGCGAAAAGCCGATTAAATGGGTAACGGAAAGCAGTCCTGGCAGCAGTaa                                                                                                  >  2:1699599/1‑150 (MQ=255)
                                                     ccGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAAt                                                                                                 >  1:1862838/1‑150 (MQ=255)
                                                     ccGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAAt                                                                                                 >  1:1999443/1‑150 (MQ=255)
                                                      cGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAAtt                                                                                                <  1:938248/150‑1 (MQ=255)
                                                        gtACCAACGGAAGTGCTCCTCGCCGTGGTCGGCGTAAACTTTTTTTCAACGGTTTGCAATTGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTa                                                                                              <  1:305671/149‑1 (MQ=255)
                                                          aCCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACg                                                                                            <  2:238046/150‑1 (MQ=255)
                                                           ccAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGc                                                                                           <  2:842728/150‑1 (MQ=255)
                                                           ccAACGGAAGTGCTACACGCCGTGGTCGGCGTAAACAGGTTTTCAACGGTTGGCAATGGGGACATCTTTACAACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCATGGCAGCAGTAATTTACGc                                                                                           >  1:1175968/1‑150 (MQ=255)
                                                            cAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCa                                                                                          >  1:849543/1‑150 (MQ=255)
                                                            cAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCa                                                                                          >  2:1674371/1‑150 (MQ=255)
                                                             aaCGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCaa                                                                                         >  2:1595663/1‑150 (MQ=255)
                                                               cGGAAGTGCTCCACGCCGTGGTAGGCGTAAACTGGTTTTCAACGGTTGGAAATGGGGACAACTTTACCACATCTTCTTCGCTGTTAAACAGCGGTCAGACGCTTAAAAGGGTAACGGTAAGCAGTCCTGGCGGCTGGATTTTACGGAAtt                                                                                       >  1:181503/1‑150 (MQ=255)
                                                                      gctagaagCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATATACGCAATTGCATCgg                                                                                <  1:107159/143‑1 (MQ=255)
                                                                      gCTCCACGCCGTGGTCGGCGTAAACTGGTGTTCGGCGGTTGGCATTGGGGCCATCTTTACCACATCTACTTCTCTGTTTAACAGCGCCCAGCCGCTTAGAAGGGTATCGGAAAGCAGTCAAGGCGTGAGTAATTTACGGATTTGCATctg                                                                                >  1:616505/1‑148 (MQ=255)
                                                                       cTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCggg                                                                               <  1:1671314/150‑1 (MQ=255)
                                                                         ccACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTc                                                                             <  2:2094257/150‑1 (MQ=255)
                                                                         ccACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTc                                                                             <  1:1407329/150‑1 (MQ=255)
                                                                           aCGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACGACATCTTCTTCGCTGTTAAACAGCGAACAGGCGGTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTccc                                                                           <  2:900122/150‑1 (MQ=255)
                                                                           aCGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTccc                                                                           <  2:1635736/150‑1 (MQ=255)
                                                                                gTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCa                                                                      >  1:2041042/1‑150 (MQ=255)
                                                                                gTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCa                                                                      <  1:1474931/150‑1 (MQ=255)
                                                                                   gTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                                   >  2:1899532/1‑150 (MQ=255)
                                                                                   gTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                                   <  2:1231113/150‑1 (MQ=255)
                                                                                   gTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGGTAAACAGCGAACAGCCGCTTAAAATGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                                   <  1:1563844/150‑1 (MQ=255)
                                                                                    gcGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAATAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                                  <  1:1907870/149‑1 (MQ=255)
                                                                                     cGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATgg                                                                 <  2:1478637/150‑1 (MQ=255)
                                                                                      ggCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGa                                                                <  1:990804/150‑1 (MQ=255)
                                                                                             aCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTATTTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAatta                                                         >  2:501511/1‑150 (MQ=255)
                                                                                             aCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattt                                                         >  2:1379699/1‑149 (MQ=255)
                                                                                             aCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCACACTCAGATGGACAAatta                                                         >  2:1710014/1‑150 (MQ=255)
                                                                                              cTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                        >  1:30813/1‑150 (MQ=255)
                                                                                                 gTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTaa                                                     >  2:626354/1‑150 (MQ=255)
                                                                                                   tttCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAAtt                                                   <  1:630560/150‑1 (MQ=255)
                                                                                                     tCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAAtttt                                                 >  2:1156818/1‑150 (MQ=255)
                                                                                                     tCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAAtttt                                                 <  2:1939964/150‑1 (MQ=255)
                                                                                                     tCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGGCCCTCTCAGATGGACAAATTATTAAtttt                                                 <  1:708903/150‑1 (MQ=255)
                                                                                                       aaCGGTTGGCAATGGTGACATCTTTACCACATCTTCTTCGCTGTTAACCAGCGAACAGCCGCTTAAAAGGGTAACGGAATGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCa                                               <  1:285879/150‑1 (MQ=255)
                                                                                                       aaCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCa                                               <  2:104139/150‑1 (MQ=255)
                                                                                                        aCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCAt                                              <  2:1494867/150‑1 (MQ=255)
                                                                                                        aCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGCTCCCTCTCAGATGGACAAATTATTAATTTACaa                                              >  1:1161460/1‑149 (MQ=255)
                                                                                                         cGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATc                                             <  2:606354/150‑1 (MQ=255)
                                                                                                                cAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGcatca                                      >  2:671350/1‑150 (MQ=255)
                                                                                                                cAATGGGGACATCTTTACCACATCATCTTCGCTGGTAAACAGCGAACAGACGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCATTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGcatca                                      <  1:1699599/150‑1 (MQ=255)
                                                                                                                 aaTGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGcatcat                                     <  2:789000/150‑1 (MQ=255)
                                                                                                                   tGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCAttt                                   >  2:1379908/1‑150 (MQ=255)
                                                                                                                       gACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCATATGGACAAATTATTAATTTTCATCTGCATCATTTCGCt                               >  1:1330914/1‑150 (MQ=255)
                                                                                                                          aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAg                            >  2:2075791/1‑150 (MQ=255)
                                                                                                                          aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAg                            <  2:1857248/150‑1 (MQ=255)
                                                                                                                           tCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTTTTAATTTTCATCTGCATAATTTCGCTCAGt                           >  1:761082/1‑150 (MQ=255)
                                                                                                                            cTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCCTCATTTCGCTCAGTg                          >  2:1529624/1‑150 (MQ=255)
                                                                                                                            cTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTg                          >  2:2114541/1‑150 (MQ=255)
                                                                                                                              ttACCACATCTTCTTCGATTTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGcc                        <  1:1026532/150‑1 (MQ=255)
                                                                                                                                acgtgaTCTTCTTCGCTTTTTAACAGCGAACAGCGGCTTTAAAGGGTTACGGATAGAAGGCCTGGCAGCAGTTATTTACGGATTTTCATCGGGTCGCTCTCTGTTTGAGTAATTATTAATTTTCTTCTTCATCATTTCGCTCAGTGCCgg                      <  2:813633/145‑1 (MQ=255)
                                                                                                                                aCCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCgg                      <  2:1287050/150‑1 (MQ=255)
                                                                                                                                aCCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCgg                      <  1:757446/150‑1 (MQ=255)
                                                                                                                                aCCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCATGGCAGCAGTAATTTACGCTATTGCATCAGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCAATTCGCTCAGTCCCgg                      >  1:304128/1‑150 (MQ=255)
                                                                                                                                  cacaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAg                    <  2:2151897/150‑1 (MQ=255)
                                                                                                                                  cacaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAg                    >  2:1316258/1‑150 (MQ=255)
                                                                                                                                   acaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGt                   <  2:365537/150‑1 (MQ=255)
                                                                                                                                    aaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTa                  <  2:849543/149‑1 (MQ=255)
                                                                                                                                     aTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTaa                 >  1:587136/1‑150 (MQ=255)
                                                                                                                                     aTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTAAGTGCCGGAGTaa                 >  2:134509/1‑150 (MQ=255)
                                                                                                                                        ttcttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACAt              >  1:1713784/1‑150 (MQ=255)
                                                                                                                                        ttcttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACAt              >  1:1518599/1‑150 (MQ=255)
                                                                                                                                         tcttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCCGTGCCGGAGTAACATc             >  1:1815938/1‑150 (MQ=255)
                                                                                                                                         tcttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATc             >  1:766673/1‑150 (MQ=255)
                                                                                                                                          cttcGCTGTTAAACAGCGAACAGACGCTTAAAAGGGTAAAGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCGTCTGCATCATTTCGCTCAGTGCCGGAGTAACATCg            <  1:1587513/150‑1 (MQ=255)
                                                                                                                                           ttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTATTTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGc           >  1:232927/1‑150 (MQ=255)
                                                                                                                                              gCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCttt        <  1:1595663/150‑1 (MQ=255)
                                                                                                                                              gCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCttt        <  1:1674124/150‑1 (MQ=255)
                                                                                                                                                tGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTc      <  1:2099107/150‑1 (MQ=255)
                                                                                                                                                    aaaCAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGc  <  2:1032504/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGC  >  NC_000913/2638436‑2638733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑