Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,673,791 A→C 5.7% intergenic (+23/+25) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,7910AC 5.7% 461.5 / 13.4 153intergenic (+23/+25)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
Reads supporting (aligned to +/- strand):  ref base A (80/64);  new base C (4/5);  total (84/69)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

Only 100 of 155 total aligned reads displayed.

CTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACA  >  NC_000913/2673642‑2673938
                                                                                                                                                     |                                                                                                                                                   
cTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGa                                                                                                                                                     >  2:2020464/1‑150 (MQ=255)
  gggATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                                   >  2:1945994/1‑150 (MQ=255)
  gggATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                                   >  2:251717/1‑150 (MQ=255)
     atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                                                                                <  1:1019575/150‑1 (MQ=255)
     atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                                                                                <  1:722434/150‑1 (MQ=255)
        tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGTAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                             >  2:1936732/1‑150 (MQ=255)
        tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                             >  2:1830611/1‑150 (MQ=255)
         gACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                            >  1:598352/1‑150 (MQ=255)
         gACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                            >  1:578142/1‑150 (MQ=255)
           ccGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                          >  2:1340116/1‑150 (MQ=255)
               cGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                                                                      <  2:455444/150‑1 (MQ=255)
               cGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                      <  2:2209495/150‑1 (MQ=255)
               cGATCCGGTTGGGCCAAATATCATTACTTTCTGGAAGAATGCCAGTATCGCTCGCGCGGCCTTGCTATTGCCAATTCCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                      <  1:2031763/150‑1 (MQ=255)
                gATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                     >  2:2109850/1‑150 (MQ=255)
                gATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                                                                                                     <  2:2007299/150‑1 (MQ=255)
                gATGCGGTGTGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCAGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                     <  2:370218/150‑1 (MQ=255)
                     ggTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaat                                                                                                                                >  2:541993/1‑150 (MQ=255)
                     ggTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaat                                                                                                                                >  2:1683602/1‑150 (MQ=255)
                         gggCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                                                                                            >  1:839677/1‑150 (MQ=255)
                           gCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTa                                                                                                                          >  2:1256270/1‑150 (MQ=255)
                           gCCAAATATGATTAATATCTGCAAGAATGTCAGTATCGCTGGCGCGTTCTAGCTTTAGCCAATTACCGCGACTCCGGCAATTTCTCACGATCGGCGATACGAAAGATGCCGCATGCGGCGTGAACTCCTATCAGGCATTTAATAAAtttc                                                                                                                         >  2:1129884/1‑148 (MQ=255)
                             cAAATATGATTAATTTCTGGAAGAATGTCAGTTTCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTAcc                                                                                                                        >  1:1620741/1‑149 (MQ=255)
                               aaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                                      <  1:251288/150‑1 (MQ=255)
                               aaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                                      <  2:614605/150‑1 (MQ=255)
                                      ttAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTa                                                                                                               <  1:188601/150‑1 (MQ=255)
                                        aaTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                             >  1:1907697/1‑150 (MQ=255)
                                        aaTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                             >  1:1506261/1‑150 (MQ=255)
                                        aaTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                             >  1:14299/1‑150 (MQ=255)
                                           ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGc                                                                                                          >  1:1723411/1‑150 (MQ=255)
                                           ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTAAATAAATTACAGCCACTCTACCCGc                                                                                                          <  2:1255462/150‑1 (MQ=255)
                                              tGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAc                                                                                                       >  1:176267/1‑150 (MQ=255)
                                              tGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTAATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAc                                                                                                       >  1:1169257/1‑150 (MQ=255)
                                               ggAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAcc                                                                                                      >  2:1743249/1‑149 (MQ=255)
                                                    aaTGTCAGTATCGCTGGCGCGAGCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCTTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtc                                                                                                 <  1:107760/150‑1 (MQ=255)
                                                      tGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtctc                                                                                               <  1:904470/150‑1 (MQ=255)
                                                        tCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGAAACGACATatctt                                                                                             >  2:1912403/1‑149 (MQ=255)
                                                         cAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGa                                                                                            >  1:1468944/1‑150 (MQ=255)
                                                          aGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGaa                                                                                           >  2:2136895/1‑150 (MQ=255)
                                                           gTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGTGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAg                                                                                          <  1:1603688/150‑1 (MQ=255)
                                                            tATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGAAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgt                                                                                         <  1:1131960/150‑1 (MQ=255)
                                                             aTCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgtt                                                                                        >  1:2135175/1‑150 (MQ=255)
                                                               cGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACATCCACTCTACCCGCAACGACATCTCTGAAgttgt                                                                                      >  2:1617085/1‑150 (MQ=255)
                                                               cGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgt                                                                                      >  1:1362350/1‑150 (MQ=255)
                                                                gCTGGCGCGTTCTTGCTATTGGCTATTACCGGGCCGGGGGCAATTTCTCTAGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgtt                                                                                     <  1:328499/150‑1 (MQ=255)
                                                                gCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGAGGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgtt                                                                                     <  2:1160674/150‑1 (MQ=255)
                                                                gCTCGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCCATCGGCGTTAGGAAAGATGCCGCATGCGGCGATAAAGCCATAACCGGCATTTAATAAAATAAAGCCACTCTAACCGCCACGAAATCTCTGAAattgtt                                                                                     >  2:1682998/1‑150 (MQ=255)
                                                                   ggCGCGTTCTTGCTATTGTCAATTTCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAt                                                                                  <  1:2032910/150‑1 (MQ=255)
                                                                     cgcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAACTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTc                                                                                >  2:706255/1‑150 (MQ=255)
                                                                      gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGATGTTGTTGATTcc                                                                               >  2:1474414/1‑150 (MQ=255)
                                                                      gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTcc                                                                               <  1:1990489/150‑1 (MQ=255)
                                                                       cgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTccc                                                                              <  1:2075518/150‑1 (MQ=255)
                                                                       cgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGACACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTccc                                                                              >  2:130547/1‑150 (MQ=255)
                                                                        gTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTcccc                                                                             <  1:1291374/150‑1 (MQ=255)
                                                                               cTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCt                                                                      <  2:607379/150‑1 (MQ=255)
                                                                               cTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCt                                                                      <  1:509502/150‑1 (MQ=255)
                                                                                tATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCCCTCTCCCCGCCACGACATCTCTGCTGTAGTTGATTCCACTGGTGCTa                                                                     >  2:1610858/1‑150 (MQ=255)
                                                                                 aTTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                                    >  2:1337564/1‑150 (MQ=255)
                                                                                  ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc                                                                   >  2:1096323/1‑150 (MQ=255)
                                                                                  ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc                                                                   <  1:769016/150‑1 (MQ=255)
                                                                                  ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCACTGGTGCTAgc                                                                   >  1:1222979/1‑150 (MQ=255)
                                                                                     gCAATTCCCGCGCAGGGGGCAATTTCTCGCGAAGGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCCACGACATCTCTGAAGTTTTTGATTCCCCTGGTGCTAGCGCa                                                                <  2:1925831/150‑1 (MQ=255)
                                                                                      cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTCCCCGCAACGACATCTCTGAAGGTGTTGATTCCCCTGGTGCTAGCGCaa                                                               >  2:266423/1‑150 (MQ=255)
                                                                                      cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACACGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCaa                                                               >  2:2084842/1‑150 (MQ=255)
                                                                                      cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGACTTATCCGGCATTTTATTAATTACAGCCAGTCTACCTCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt                                                              >  2:1361690/1‑150 (MQ=255)
                                                                                       aaTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATATCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt                                                              >  2:1987704/1‑150 (MQ=255)
                                                                                        tttACCGGGCCGGGGGCAATTTCTCTCGATCTGCGTTAGGAAAGATGCAGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATg                                                             <  1:108733/149‑1 (MQ=255)
                                                                                          tACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATgag                                                           <  2:1466524/150‑1 (MQ=255)
                                                                                            ccgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGATGATTCCCCTGGTGCTAGCGCAATGAGAt                                                         <  2:2158636/150‑1 (MQ=255)
                                                                                             cgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATc                                                        <  2:2071045/150‑1 (MQ=255)
                                                                                              gggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCa                                                       <  1:1193317/150‑1 (MQ=255)
                                                                                                gccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAcc                                                     >  2:484011/1‑150 (MQ=255)
                                                                                                 ccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAccc                                                    <  1:497209/150‑1 (MQ=255)
                                                                                                     gggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATAACCCCCtt                                                >  1:772842/1‑150 (MQ=255)
                                                                                                           tttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGc                                          <  1:1945994/150‑1 (MQ=255)
                                                                                                            ttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCTTCCGGGCga                                        <  1:2071561/150‑1 (MQ=255)
                                                                                                              ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGCCATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                       >  1:1013269/1‑150 (MQ=255)
                                                                                                              ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                       >  2:1124750/1‑150 (MQ=255)
                                                                                                              ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                       <  1:1983475/150‑1 (MQ=255)
                                                                                                               tctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatg                                      <  2:1024999/150‑1 (MQ=255)
                                                                                                                ctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTACGGGCgatga                                     >  2:1080413/1‑150 (MQ=255)
                                                                                                                ctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATCTAATAAATTACAGCCACTCTACACGCAACGACATCACTGAAGTTGTTTATTCACCTGGTGCTATCGCAAAGAGATAACCCACTTCCAGGCgatga                                     >  1:409372/1‑150 (MQ=255)
                                                                                                                 tcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAATGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgat                                    <  2:1923735/150‑1 (MQ=255)
                                                                                                                   gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCTGCATTTAATAAATTACAGCCTCTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                                  <  1:251717/150‑1 (MQ=255)
                                                                                                                         cGTTAGGATAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATcc                            <  2:1118109/150‑1 (MQ=255)
                                                                                                                         cGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGACACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCATCCGGGCGATGATGATCATcc                            >  2:355586/1‑150 (MQ=255)
                                                                                                                            tAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTCCCCGCAACGACATCTATGAAGTTGTTGATTCCCCTGGTGCTAGCGAGATGAGATAACCCCCTTCCGGGCGATGATGATCATCCGgc                         >  2:47343/1‑150 (MQ=255)
                                                                                                                              ggATAGATGCCGGATGCGGCGTGAACGACTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                       <  2:801833/150‑1 (MQ=255)
                                                                                                                              ggAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                       >  2:540973/1‑150 (MQ=255)
                                                                                                                              ggAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGGTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                       >  1:1341039/1‑150 (MQ=255)
                                                                                                                                   aaTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGc                  >  1:1001820/2‑150 (MQ=255)
                                                                                                                                     tGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCGCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTc                >  2:136966/1‑150 (MQ=255)
                                                                                                                                      gCCGGATGCGGCGTGATCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCATCGACATCTCGGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGATATCACACCCTGACGGGCGATGATGATAATCGGGCGCATGGATCa               >  1:1011173/1‑150 (MQ=255)
                                                                                                                                       ccGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAt              >  1:1673620/1‑150 (MQ=255)
                                                                                                                                        cgtaTGCGGCGTGAACGCCTTATCCGTCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTGTGAAGTTGTTGATTCCCCTGGGGCTATCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATc             <  1:578320/147‑1 (MQ=255)
                                                                                                                                        cGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATc             <  1:2109850/150‑1 (MQ=255)
                                                                                                                                         ggataaggcgTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCg            >  1:456165/7‑150 (MQ=255)
                                                                                                                                              cggcgTGAACGCCTTATCCGGCATTTAAAAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTcc       <  1:1936732/150‑1 (MQ=255)
                                                                                                                                               ggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCca      <  2:839677/150‑1 (MQ=255)
                                                                                                                                                gcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCCCTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcag     >  1:1571115/1‑150 (MQ=255)
                                                                                                                                                gcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcaa     <  1:2100211/150‑2 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACA  >  NC_000913/2673642‑2673938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: