Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,673,798 T→C 5.3% intergenic (+30/+18) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,7980TC 5.3% 472.0 / 11.3 153intergenic (+30/+18)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
Reads supporting (aligned to +/- strand):  ref base T (79/65);  new base C (4/4);  total (84/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01

Only 100 of 153 total aligned reads displayed.

TGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCA  >  NC_000913/2673650‑2673948
                                                                                                                                                    |                                                                                                                                                      
tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGTAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                                       >  2:1936732/1‑150 (MQ=255)
tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                                       >  2:1830611/1‑150 (MQ=255)
 gACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                                      >  2:200767/1‑150 (MQ=255)
      gCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                                                 <  1:988999/150‑1 (MQ=255)
       cGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                                                                                <  2:455444/150‑1 (MQ=255)
       cGATCCGGTTGGGCCAAATATCATTACTTTCTGGAAGAATGCCAGTATCGCTCGCGCGGCCTTGCTATTGCCAATTCCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                                <  1:2031763/150‑1 (MQ=255)
        gATGCGGTTGTGCCAAATATGATTAATTTCTGTAAGAATGGCAGTATCGCTGGCGCGTTCTTGCTATTGGCAAATACCGGGCCGGGGGCAATTTCTCGCGAACGGAGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                               >  1:1183165/1‑150 (MQ=255)
        gATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                               >  2:2109850/1‑150 (MQ=255)
        gATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                                                                                                               <  2:2007299/150‑1 (MQ=255)
        gATGCGGTGTGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCAGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                               <  2:370218/150‑1 (MQ=255)
             ggTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaat                                                                                                                                          >  2:541993/1‑150 (MQ=255)
             ggTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaat                                                                                                                                          >  2:1683602/1‑150 (MQ=255)
                   gCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTa                                                                                                                                    >  2:191811/1‑150 (MQ=255)
                   gCCAAATATGATTAATATCTGCAAGAATGTCAGTATCGCTGGCGCGTTCTAGCTTTAGCCAATTACCGCGACTCCGGCAATTTCTCACGATCGGCGATACGAAAGATGCCGCATGCGGCGTGAACTCCTATCAGGCATTTAATAAAtttc                                                                                                                                   >  2:1129884/1‑148 (MQ=255)
                       aaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                                                <  2:614605/150‑1 (MQ=255)
                       aaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                                                <  1:251288/150‑1 (MQ=255)
                           tGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCAct                                                                                                                            >  1:780847/1‑150 (MQ=255)
                              ttAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTa                                                                                                                         >  2:1983829/1‑150 (MQ=255)
                              ttAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTa                                                                                                                         <  1:188601/150‑1 (MQ=255)
                                aaTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                                       >  1:1506261/1‑150 (MQ=255)
                                aaTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                                       >  1:14299/1‑150 (MQ=255)
                                aaTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                                       >  1:1907697/1‑150 (MQ=255)
                                  tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGTGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCg                                                                                                                     <  1:1402187/150‑1 (MQ=255)
                                   ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGc                                                                                                                    >  1:1723411/1‑150 (MQ=255)
                                   ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGc                                                                                                                    >  1:2102301/1‑150 (MQ=255)
                                   ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTAAATAAATTACAGCCACTCTACCCGc                                                                                                                    <  2:1255462/150‑1 (MQ=255)
                                      tGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAc                                                                                                                 >  1:176267/1‑150 (MQ=255)
                                      tGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTAATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAc                                                                                                                 >  1:1169257/1‑150 (MQ=255)
                                         aagaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGAc                                                                                                              >  1:598120/1‑150 (MQ=255)
                                          agaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACa                                                                                                             >  1:737581/1‑150 (MQ=255)
                                              tGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtctc                                                                                                         <  1:904470/150‑1 (MQ=255)
                                                tCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTg                                                                                                       <  2:243104/150‑1 (MQ=255)
                                                tCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGAAACGACATatctt                                                                                                       >  2:1912403/1‑149 (MQ=255)
                                                  aGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGaa                                                                                                     >  2:2136895/1‑150 (MQ=255)
                                                   gTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGTGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAg                                                                                                    <  1:1603688/150‑1 (MQ=255)
                                                   gTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAACCGCAACGACATCTCTGAAg                                                                                                    >  2:1343976/1‑150 (MQ=255)
                                                   gTATCGCTGGCGCGGTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAg                                                                                                    <  1:163175/150‑1 (MQ=255)
                                                     aTCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgtt                                                                                                  >  1:2135175/1‑150 (MQ=255)
                                                       cGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgt                                                                                                >  2:1125567/1‑150 (MQ=255)
                                                        gCTGGCGCGTTCTTGCTATTGGCTATTACCGGGCCGGGGGCAATTTCTCTAGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgtt                                                                                               <  1:328499/150‑1 (MQ=255)
                                                         cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTTAACGCCTTATCCTGCATTTAATAAATTACAGCCACTCTACCCGAAACGACATCTCTGAAGttgttg                                                                                              >  2:1814117/1‑150 (MQ=255)
                                                         cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGttgttg                                                                                              >  2:152648/1‑150 (MQ=255)
                                                           ggCGCGTTCTTGCTATTGTCAATTTCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAt                                                                                            <  1:2032910/150‑1 (MQ=255)
                                                              gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTcc                                                                                         <  1:1990489/150‑1 (MQ=255)
                                                              gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCATTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTcc                                                                                         <  2:1928867/150‑1 (MQ=255)
                                                                     tGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg                                                                                  >  2:80244/1‑150 (MQ=255)
                                                                       cTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCt                                                                                <  1:509502/150‑1 (MQ=255)
                                                                        tATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCCCTCTCCCCGCCACGACATCTCTGCTGTAGTTGATTCCACTGGTGCTa                                                                               >  2:1610858/1‑150 (MQ=255)
                                                                         aTTTGCAATTTCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGATAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCTTTTAATAAATTACAGCCACTCTACCGGCAACGAGATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                                              <  1:1260461/150‑1 (MQ=255)
                                                                         aTTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                                              >  2:1337564/1‑150 (MQ=255)
                                                                          ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc                                                                             <  1:769016/150‑1 (MQ=255)
                                                                          ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc                                                                             >  2:1096323/1‑150 (MQ=255)
                                                                          ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCACTGGTGCTAgc                                                                             >  1:1222979/1‑150 (MQ=255)
                                                                            ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc                                                                           >  2:470349/1‑150 (MQ=255)
                                                                             gCAATTCCCGCGCAGGGGGCAATTTCTCGCGAAGGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCCACGACATCTCTGAAGTTTTTGATTCCCCTGGTGCTAGCGCa                                                                          <  2:1925831/150‑1 (MQ=255)
                                                                              cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTCCCCGCAACGACATCTCTGAAGGTGTTGATTCCCCTGGTGCTAGCGCaa                                                                         >  2:266423/1‑150 (MQ=255)
                                                                              cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACACGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCaa                                                                         >  2:2084842/1‑150 (MQ=255)
                                                                              cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGACTTATCCGGCATTTTATTAATTACAGCCAGTCTACCTCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt                                                                        >  2:1361690/1‑150 (MQ=255)
                                                                                tttACCGGGCCGGGGGCAATTTCTCTCGATCTGCGTTAGGAAAGATGCAGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATg                                                                       <  1:108733/149‑1 (MQ=255)
                                                                                  tACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATgag                                                                     <  2:1466524/150‑1 (MQ=255)
                                                                                    ccgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGAt                                                                   <  1:2074934/150‑1 (MQ=255)
                                                                                    ccgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGATGATTCCCCTGGTGCTAGCGCAATGAGAt                                                                   <  2:2158636/150‑1 (MQ=255)
                                                                                     cgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATc                                                                  <  2:2071045/150‑1 (MQ=255)
                                                                                     cgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATc                                                                  >  1:458158/1‑150 (MQ=255)
                                                                                        gccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAcc                                                               >  2:484011/1‑150 (MQ=255)
                                                                                                   tttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGc                                                    <  1:1945994/150‑1 (MQ=255)
                                                                                                   tttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGc                                                    <  1:1340116/150‑1 (MQ=255)
                                                                                                   tttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGc                                                    <  2:517395/150‑1 (MQ=255)
                                                                                                    ttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCTTCCGGGCga                                                  <  1:2071561/150‑1 (MQ=255)
                                                                                                      ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGCCATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                                 >  1:1013269/1‑150 (MQ=255)
                                                                                                      ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                                 <  1:1983475/150‑1 (MQ=255)
                                                                                                      ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                                 >  2:1723220/1‑150 (MQ=255)
                                                                                                        ctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATCTAATAAATTACAGCCACTCTACACGCAACGACATCACTGAAGTTGTTTATTCACCTGGTGCTATCGCAAAGAGATAACCCACTTCCAGGCgatga                                               >  1:409372/1‑150 (MQ=255)
                                                                                                         tcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgat                                              >  2:137979/1‑150 (MQ=255)
                                                                                                          cGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                                             <  2:1187200/150‑1 (MQ=255)
                                                                                                           gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCTGCATTTAATAAATTACAGCCTCTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                                            <  1:251717/150‑1 (MQ=255)
                                                                                                                 cGTTAGGATAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATcc                                      <  2:1118109/150‑1 (MQ=255)
                                                                                                                 cGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGACACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCATCCGGGCGATGATGATCATcc                                      >  2:355586/1‑150 (MQ=255)
                                                                                                                   ttAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCgg                                    >  2:677161/1‑150 (MQ=255)
                                                                                                                      ggATAGATGCCGGATGCGGCGTGAACGACTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                                 <  2:801833/150‑1 (MQ=255)
                                                                                                                      ggAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGGTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                                 >  1:1341039/1‑150 (MQ=255)
                                                                                                                           gATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGc                            <  2:598120/150‑1 (MQ=255)
                                                                                                                           aaTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGc                            >  1:1001820/2‑150 (MQ=255)
                                                                                                                             tGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTc                          >  2:1320579/1‑150 (MQ=255)
                                                                                                                              gCCGGATGCGGCGTGATCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCATCGACATCTCGGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGATATCACACCCTGACGGGCGATGATGATAATCGGGCGCATGGATCa                         >  1:1011173/1‑150 (MQ=255)
                                                                                                                              gCCGGATGCGGCGTGAACGCCTTATCCGGCAGTTTATAAATTACAGCCACTCTACCCGCAACGACATCTCTGCAGGTGTTGCTTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGAGGATGATCATCCGGCGCATGGCTCa                         >  1:1019830/1‑150 (MQ=255)
                                                                                                                              gCCGGAAGCGGCGTGCACGCCTTATCCGGCATTTAATAAATTACTGCCACTCTACCCGCAACCACATCACTGAAGTTGTTGTTTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCa                         <  2:164395/150‑1 (MQ=255)
                                                                                                                               ccGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAt                        >  1:1673620/1‑150 (MQ=255)
                                                                                                                                cgtaTGCGGCGTGAACGCCTTATCCGTCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTGTGAAGTTGTTGATTCCCCTGGGGCTATCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATc                       <  1:578320/147‑1 (MQ=255)
                                                                                                                                 ggataaggcgTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCg                      >  1:456165/7‑150 (MQ=255)
                                                                                                                                   aTGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGt                    <  2:1282508/150‑1 (MQ=255)
                                                                                                                                     gcggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTc                  <  1:541993/150‑1 (MQ=255)
                                                                                                                                      cggcgTGAACGCCTTATCCGGCATTTAAAAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTcc                 <  1:1936732/150‑1 (MQ=255)
                                                                                                                                       ggcgTGAACGCCTTATCCGGCATTTAATAATTTACAGCCACTCTACCCGCAACGACTTCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGAGGATGATCATCCGGCGCCTGGCTCATCGGGTCca                >  2:1050757/1‑150 (MQ=255)
                                                                                                                                       ggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCca                <  2:839677/150‑1 (MQ=255)
                                                                                                                                        gcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCCCTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcag               >  1:1571115/1‑150 (MQ=255)
                                                                                                                                        gcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcaa               <  1:2100211/150‑2 (MQ=255)
                                                                                                                                           tGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcagaca            <  1:1096323/150‑1 (MQ=255)
                                                                                                                                              aCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCt                                                                                <  1:834115/79‑1 (MQ=255)
                                                                                                                                                  cTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGC‑‑‑GATGATCATCCGGCGCAGCGATCATCAGCTCCTGAAAcaagaacaca  >  2:1288541/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCA  >  NC_000913/2673650‑2673948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: