Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,800,519 T→G 5.9% intergenic (+44/‑204) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,800,5190TG 5.9% 354.6 / 7.9 122intergenic (+44/‑204)ygaM/nrdHputative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
Reads supporting (aligned to +/- strand):  ref base T (54/60);  new base G (2/6);  total (56/66)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGA  >  NC_000913/2800372‑2800668
                                                                                                                                                   |                                                                                                                                                     
gCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                                                                                     <  2:713466/150‑1 (MQ=255)
 cTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCtt                                                                                                                                                    >  1:2195345/1‑150 (MQ=255)
 cTGCGCGGAATCTTTAGATCCAGAAAGCCCCTGCTGTAGCGAGAGTACACCAGCTGCGGTAGGTAATTATATCGGCGCACAATTAAGCATGCGCAAAACTAAGAGCAAAAATGATAATAAAAAAGCGTCTTTGACCCCGAAGCCTGTCtt                                                                                                                                                    >  1:1144988/1‑150 (MQ=255)
  tGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCATCTGCGGTATGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGGCTTc                                                                                                                                                   <  2:429006/150‑1 (MQ=255)
     gcgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTCCGGTAGGTATTTTTATAGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAAAAAATACTCGTCTTTGACCCAGAAGCATGTCTTCggg                                                                                                                                                >  1:1800630/1‑150 (MQ=255)
      cgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCgggg                                                                                                                                               >  1:65889/1‑150 (MQ=255)
       ggATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt                                                                                                                                              <  2:1894429/150‑1 (MQ=255)
       ggATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt                                                                                                                                              <  2:1748596/150‑1 (MQ=255)
        gATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGtt                                                                                                                                             <  1:552765/150‑1 (MQ=255)
           tCTTTTGTTCGTGTAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTATGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGtcaaa                                                                                                                                          <  2:620387/150‑5 (MQ=255)
             ttttGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttt                                                                                                                                        >  2:1667819/1‑150 (MQ=255)
               ttGTTCGTGAAAGACCGTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTTTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTGCGGGGTTTCttttt                                                                                                                                      <  1:1130587/150‑1 (MQ=255)
               ttGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTAATTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGGCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttttt                                                                                                                                      <  1:1426369/150‑1 (MQ=255)
                tGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg                                                                                                                                     <  2:1565368/150‑1 (MQ=255)
                tGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg                                                                                                                                     >  2:1025893/1‑150 (MQ=255)
                  ttCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGTTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc                                                                                                                                   <  2:1235367/150‑1 (MQ=255)
                    cGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTg                                                                                                                                 >  1:1795620/1‑150 (MQ=255)
                    cGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTg                                                                                                                                 <  1:1016637/150‑1 (MQ=255)
                      tGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGt                                                                                                                               <  1:513347/150‑1 (MQ=255)
                       taaaGACCCTGGTGTAGCGTGGGTACAGCAGCTGAGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTg                                                                                                                              <  2:2199310/149‑1 (MQ=255)
                        aaaGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTCGTGa                                                                                                                             >  2:1477013/1‑150 (MQ=255)
                        aaaGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCAAGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGa                                                                                                                             <  1:890528/150‑1 (MQ=255)
                           gACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATc                                                                                                                          <  2:746310/150‑1 (MQ=255)
                            aCCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATca                                                                                                                         <  2:954091/150‑1 (MQ=255)
                             cccTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                                                                                        <  1:1811618/150‑1 (MQ=255)
                                tGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                                                                                     <  2:1739650/150‑1 (MQ=255)
                                    gtAGCGAGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATc                                                                                                                 >  2:904124/1‑150 (MQ=255)
                                       gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcccc                                                                                                              >  2:2012315/1‑150 (MQ=255)
                                            ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                                                                                         <  1:1893680/150‑1 (MQ=255)
                                            gctACACCAGCTGAGGTAGGTATTTTTATCCTCGCACTTTTCAGCATGCGAAAATCGTAGTGCAAAAATGATACTAAATACGCTTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                                                                                         <  2:417835/148‑1 (MQ=255)
                                             gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAccc                                                                                                        >  1:691227/1‑150 (MQ=255)
                                              tACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcccc                                                                                                       >  1:2007361/1‑150 (MQ=255)
                                               aCAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCg                                                                                                      >  2:1255972/1‑150 (MQ=255)
                                                cagcagGTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGTGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGt                                                                                                     <  1:451540/150‑1 (MQ=255)
                                                  gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                                                                                   >  1:454052/1‑150 (MQ=255)
                                                      cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCt                                                                                               <  1:533065/150‑1 (MQ=255)
                                                       tGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc                                                                                              >  1:1255522/1‑150 (MQ=255)
                                                        gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                                                                             >  1:385700/1‑150 (MQ=255)
                                                           gtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATACAAATACACCGTCACTCTCatat                                                                                          >  2:917239/1‑150 (MQ=255)
                                                             aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATcc                                                                                        >  2:507043/1‑150 (MQ=255)
                                                              ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                                                                             >  2:1066817/1‑144 (MQ=255)
                                                              ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                                                                             <  1:1066817/144‑1 (MQ=255)
                                                                taTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAgg                                                                                     <  2:1762587/150‑1 (MQ=255)
                                                                  tttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGt                                                                                   >  2:606384/1‑150 (MQ=255)
                                                                  tttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCACCATACACCGTAACGCTCATATACAGGGt                                                                                   >  1:358009/1‑150 (MQ=255)
                                                                  tttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCGTTTTGCCTGGTGAATCACAAAACTCCCCCTACCCCGTCACGCTCATATCCAGGGt                                                                                   >  2:1047630/1‑150 (MQ=255)
                                                                      tATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAAtt                                                                               >  1:14129/1‑150 (MQ=255)
                                                                       aTCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAAttt                                                                              >  2:601129/1‑150 (MQ=255)
                                                                       aTCGGCGCACTGGTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAAttt                                                                              <  1:1076418/150‑1 (MQ=255)
                                                                        tCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCGCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTc                                                                             <  1:1554464/150‑1 (MQ=255)
                                                                        tCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCACGGCACGCTCATATCAAGGGTAATTTc                                                                             >  1:327548/1‑150 (MQ=255)
                                                                         cGGCGCACTGTTAAGCATGCGCAAATCGTAGTGGAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCg                                                                            <  1:1961719/150‑1 (MQ=255)
                                                                           gcgcACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                                                                          <  2:901583/150‑1 (MQ=255)
                                                                                  gTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAtt                                                                   >  2:1654982/1‑150 (MQ=255)
                                                                                  gTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGAAATTTCGACCACTAtt                                                                   >  1:1187769/1‑150 (MQ=255)
                                                                                      tgCATGCGCAAATCGTAGTGCAAAAATGAAAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt                                                               <  2:288311/149‑1 (MQ=255)
                                                                                      aGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt                                                               <  1:870604/150‑1 (MQ=255)
                                                                                        cATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCtat                                                             <  1:2139227/150‑1 (MQ=255)
                                                                                          tGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCtatat                                                           >  1:893231/1‑150 (MQ=255)
                                                                                          tGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGGCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCCCTATTTGCtatat                                                           >  2:360375/1‑150 (MQ=255)
                                                                                            cgcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGTGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATATCGACAACTATTTGCTatatat                                                         >  1:1048619/1‑150 (MQ=255)
                                                                                            cgcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTatatat                                                         >  2:717539/1‑150 (MQ=255)
                                                                                            cgcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTatatat                                                         >  1:1793058/1‑150 (MQ=255)
                                                                                            cgcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCACGTCACGCTCATATCCAGTGTAATTTCGACCACTATTTGCTatatat                                                         <  1:1477013/150‑1 (MQ=255)
                                                                                               aaaTCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGACGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATtgt                                                      >  2:219192/1‑150 (MQ=255)
                                                                                                   cGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTgg                                                  <  1:983078/150‑1 (MQ=255)
                                                                                                    gTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACGACTATTTGCTATATATTGTGTCGt                                                 >  2:1412953/1‑150 (MQ=255)
                                                                                                     tAGTGCAAAAATGATAATAAATACGCGTCTTTGCCCCCGAAGCCTGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGtt                                                <  2:367308/150‑1 (MQ=255)
                                                                                                      aGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCTGTGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTg                                               <  1:904124/150‑1 (MQ=255)
                                                                                                      aGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTg                                               <  1:1607727/150‑1 (MQ=255)
                                                                                                       gTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGa                                              <  2:2112320/150‑1 (MQ=255)
                                                                                                        tGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGaa                                             >  2:1429442/1‑150 (MQ=255)
                                                                                                         gCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAAt                                            >  2:1669570/1‑150 (MQ=255)
                                                                                                         gCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAAt                                            >  1:444963/1‑150 (MQ=255)
                                                                                                             aaaTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCttt                                        <  2:454052/150‑1 (MQ=255)
                                                                                                               aTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCAAACCCCGACACGCTCATATCAAGGGTAATTACGACCAATATTTGATTTATATTGTGTGTTTGAATAttttt                                      >  2:793607/1‑150 (MQ=255)
                                                                                                                tGATAATAAATACGCGTCTATGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTCCCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCtttttt                                     <  2:380343/150‑1 (MQ=255)
                                                                                                                  ataataAATACGCGTCTTTGACCCCGAAGCCTGTCTTCTGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCa                                   <  2:1640755/150‑1 (MQ=255)
                                                                                                                   taataaATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCaa                                  <  1:1429442/150‑1 (MQ=255)
                                                                                                                      taaataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTa                               <  1:994116/150‑1 (MQ=255)
                                                                                                                        aataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCCCGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACa                             >  1:2066806/1‑150 (MQ=255)
                                                                                                                        aataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACa                             <  2:1269675/150‑1 (MQ=255)
                                                                                                                         ataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACAt                            <  2:1647546/150‑1 (MQ=255)
                                                                                                                             gcgTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAg                        <  1:1456048/150‑1 (MQ=255)
                                                                                                                              cgTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATAATGTGTGGTTGAATCTTTTTTCACCTACATCTAGt                       >  1:345132/1‑150 (MQ=255)
                                                                                                                                     gACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTg                <  1:1936050/150‑1 (MQ=255)
                                                                                                                                       ccccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTa              <  2:617121/150‑1 (MQ=255)
                                                                                                                                       ccccGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTGCCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTa              >  1:2128317/1‑150 (MQ=255)
                                                                                                                                       ccccGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTa              >  2:428904/1‑150 (MQ=255)
                                                                                                                                         ccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTAta            >  1:1391406/1‑149 (MQ=255)
                                                                                                                                         ccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATc            >  1:1070720/1‑150 (MQ=255)
                                                                                                                                         ccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATc            <  2:1274551/150‑1 (MQ=255)
                                                                                                                                          cGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACAACATCTAGTATCTCTGTATCa           <  1:1667819/150‑1 (MQ=255)
                                                                                                                                            aaGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAACTCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAAc         <  2:303096/150‑1 (MQ=255)
                                                                                                                                            aaGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCGCCCTTCACCGTAACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAAc         <  2:1800630/150‑1 (MQ=255)
                                                                                                                                            aaGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAAc         <  1:601129/150‑1 (MQ=255)
                                                                                                                                              gCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACag       >  2:1968101/1‑150 (MQ=255)
                                                                                                                                                cTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACagag     <  2:358009/150‑1 (MQ=255)
                                                                                                                                                 tGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACagaga    <  2:2007361/150‑1 (MQ=255)
                                                                                                                                                  gTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACagagag   <  1:1968101/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGA  >  NC_000913/2800372‑2800668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: