Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,804,535 A→T 10.1% intergenic (+74/‑280) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,804,5350AT10.1% 233.0 / 8.7 97intergenic (+74/‑280)nrdF/proVribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (52/34);  new base T (2/9);  total (54/43)
Fisher's exact test for biased strand distribution p-value = 1.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGC  >  NC_000913/2804386‑2804683
                                                                                                                                                      |                                                                                                                                                    
tttCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCaaa                                                                                                                                                      >  1:2150318/1‑150 (MQ=255)
tttCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCaaa                                                                                                                                                      <  1:1958249/150‑1 (MQ=255)
   cTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAaataa                                                                                                                                                   >  2:1139823/1‑150 (MQ=255)
   cTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAaataa                                                                                                                                                   >  2:1548189/1‑150 (MQ=255)
    ttttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACACAaataat                                                                                                                                                  <  2:1848352/150‑1 (MQ=255)
     tttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAAtt                                                                                                                                                 <  2:647946/150‑1 (MQ=255)
     tttCCGGATCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCATCAGGGAAATAATTCCCGCAAAATAGCTTTTTATCAAGCAAATAAtt                                                                                                                                                 >  2:105013/1‑150 (MQ=255)
      ttCCGTTTCAAGCTCCTCTTATGTGATGGGGAAAGAGGTTGAAACAGAAGATGAAGACTGTAATTTCTGAGGGTTTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAAttt                                                                                                                                                <  2:1143585/150‑1 (MQ=255)
       tCCGGTTCTGGCTCCTCTAATGAGATGGTGAAAGAGTTTGAAACAGAAGATGACGACTGGAATTTCTGAGGGTGGTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtg                                                                                                                                               <  2:1344256/150‑1 (MQ=255)
       tCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtg                                                                                                                                               <  1:1746780/150‑1 (MQ=255)
        ccGGTTCAGGCTCCTCTTATGTGAAGGGTAAAGCGGTAGAAACACAAGCTGAAGACTCGAATTTCAGAGGGTGTAATTTTCAAAAATATCACTAACCGCACCACGGAAATAATTCCCGCAAAAAAGCTTTTTATCACGCAAATAATTtga                                                                                                                                              >  1:1823085/1‑149 (MQ=255)
          ggTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtgg                                                                                                                                            >  2:154801/1‑150 (MQ=255)
           gTTCAGGCTCATCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCACAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggt                                                                                                                                           >  2:2197270/1‑150 (MQ=255)
             tCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGa                                                                                                                                         >  2:859909/1‑150 (MQ=255)
              cAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAt                                                                                                                                        <  1:1650678/150‑1 (MQ=255)
               aGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAta                                                                                                                                       >  1:1795759/1‑149 (MQ=255)
               aGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATc                                                                                                                                       <  1:1671787/150‑1 (MQ=255)
                ggCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCt                                                                                                                                      <  2:263079/150‑1 (MQ=255)
                ggCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCt                                                                                                                                      >  2:909504/1‑150 (MQ=255)
                   tcctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTaca                                                                                                                                   <  2:481329/150‑1 (MQ=255)
                   tcctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTaca                                                                                                                                   >  2:832989/1‑150 (MQ=255)
                     ctctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACt                                                                                                                                 <  1:652132/150‑1 (MQ=255)
                            gtgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATActc                                                                                                                          >  1:1832311/1‑150 (MQ=255)
                             tgtaggggAAAGCGGTTGAAACAGAAGATCAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATATTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATActct                                                                                                                         <  2:560871/146‑1 (MQ=255)
                             tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTTTGGTGATCTACACTGATActct                                                                                                                         >  2:609469/1‑150 (MQ=255)
                               aTGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGt                                                                                                                       >  2:1247729/1‑150 (MQ=255)
                                  gggAAATCCGTTGAAACAGAAGATGAAGACTGGAATATCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCACGAGAATTTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGc                                                                                                                    <  2:418584/150‑1 (MQ=255)
                                  gggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGc                                                                                                                    >  1:485531/1‑150 (MQ=255)
                                       aGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCattat                                                                                                               >  2:68151/1‑150 (MQ=255)
                                        gCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCAAGCAAATAATTTGTGGTGATCTACACTGATACTATGTTGCattatt                                                                                                              >  1:1589113/1‑150 (MQ=255)
                                         cGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTc                                                                                                             >  2:1122715/1‑150 (MQ=255)
                                          ggTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCg                                                                                                            >  1:705015/1‑150 (MQ=255)
                                           gTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGc                                                                                                           <  1:990535/150‑1 (MQ=255)
                                            ttGAAACAGAAGATGAAGACTTGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCTAAATAGTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGcc                                                                                                          <  2:1814132/150‑1 (MQ=255)
                                            ttGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGcc                                                                                                          <  1:2051538/150‑1 (MQ=255)
                                              gAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGGTGCATTATTCGCCTg                                                                                                        <  1:1988322/150‑1 (MQ=255)
                                              gAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACAACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTg                                                                                                        >  2:1728262/1‑150 (MQ=255)
                                              gAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAAAATCACTACCAGCAGCAGGGAAATAAATCCCACAAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGcaag                                                                                                        >  1:372690/1‑147 (MQ=255)
                                                   agaagaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCATGAAAcc                                                                                                   >  1:1784936/1‑150 (MQ=255)
                                                    gaagaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCCCGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACca                                                                                                  >  1:1676202/1‑150 (MQ=255)
                                                       gaTGAAGACTGGACTTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACaa                                                                                               <  2:372690/150‑1 (MQ=255)
                                                       gaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACaa                                                                                               >  1:195500/1‑150 (MQ=255)
                                                         tGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAata                                                                                             >  2:2062684/1‑150 (MQ=255)
                                                               cTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAATTAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                       >  2:1228255/1‑150 (MQ=255)
                                                               cTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                       >  2:261580/1‑150 (MQ=255)
                                                                 ggAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCTCCTGAAACCACAATATTCAGGCg                                                                                     >  2:1814913/1‑150 (MQ=255)
                                                                 ggAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCg                                                                                     >  2:1206099/1‑150 (MQ=255)
                                                                   aaTTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGtt                                                                                   >  2:1484827/1‑150 (MQ=255)
                                                                   aaTTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCATGAAACCACAATATTCAGGCgat                                                                                   >  2:120212/1‑148 (MQ=255)
                                                                     tttCTGAGGGTGTTATTTTCAAAAATATCACAACCCGCAGCAGGGAAATAATTCCCGCCAAATAGGTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGtttt                                                                                 <  1:651020/150‑1 (MQ=255)
                                                                      ttCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGttttt                                                                                >  1:565584/1‑150 (MQ=255)
                                                                       tCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAAATCCCGCCAAATAGCTTTTTATCACGCAAATAAGTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGtttttt                                                                               <  1:2130848/150‑1 (MQ=255)
                                                                        cTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTc                                                                              <  1:2139973/150‑1 (MQ=255)
                                                                         tGAGGTTTTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCg                                                                             <  1:1981348/150‑1 (MQ=255)
                                                                               tgtTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGACAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCt                                                                       <  2:2136890/150‑1 (MQ=255)
                                                                                gtTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCtt                                                                      >  1:1209914/1‑150 (MQ=255)
                                                                                  tATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTg                                                                    >  1:501657/1‑150 (MQ=255)
                                                                                    ttttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGAc                                                                  <  1:776382/150‑1 (MQ=255)
                                                                                      ttCAAAAATCTCTCTACCCGACGCAGGGAAATTATTCGCCCGAATTTTTTTTTTTTATGTTAATTAGTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGACTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaa                                                                <  2:1364898/150‑1 (MQ=255)
                                                                                          aaaaTATCACTACCCGCAGCTGGGAAATAATTCCCGCAAATTTTTTTTTTTTATGTTAATTATTTTTTTGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaaaa                                                            <  2:592926/150‑1 (MQ=255)
                                                                                           aaaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCATATAGCTTTTTATCACGCAATTAATTTGAGGTGATCTACACTGATACTCTGTTGAATTATACGCCTGAAACCTGAATATACAGGCGTTTTTTCGCTATCTTTGACAAAatat                                                           >  1:1160687/1‑150 (MQ=255)
                                                                                              tatCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTTGCTATCTTTGACAAAAAATATc                                                        <  1:1932704/150‑1 (MQ=255)
                                                                                               atCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCa                                                       <  1:2154999/150‑1 (MQ=255)
                                                                                               atCAATAACCGCAGCAGGGAAATAATTCCCGCAAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTCTTTCGCTATCTTTGACAAAAAATATCa                                                       <  1:1047746/150‑1 (MQ=255)
                                                                                                tCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCaa                                                      >  1:1101155/1‑150 (MQ=255)
                                                                                                 cACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAAAAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAAAATTCAGGCGTTTTTTCGCTATCTATGACAAAAAATATCaaa                                                     >  2:2050034/1‑149 (MQ=255)
                                                                                                  aCTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCTCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACt                                                    >  2:311014/1‑150 (MQ=255)
                                                                                                  aCTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACt                                                    <  1:1122715/150‑1 (MQ=255)
                                                                                                   cTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTAAAACGCAAATAATTTCTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCACGCGTTTTTTCGCTATCTTAGACAAAAAATATCAACtt                                                   >  2:1120820/1‑150 (MQ=255)
                                                                                                      cccGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCATATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtct                                                <  1:1867650/150‑1 (MQ=255)
                                                                                                        cGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtctcc                                              >  1:1347153/1‑149 (MQ=255)
                                                                                                        cGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCg                                              <  1:2195696/150‑1 (MQ=255)
                                                                                                          cagcagGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGAt                                            <  1:203964/150‑1 (MQ=255)
                                                                                                              agGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAGCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGc                                        <  1:1172949/150‑1 (MQ=255)
                                                                                                                ggAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGctc                                      >  1:524667/1‑150 (MQ=255)
                                                                                                                   aataatTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCa                                   >  2:1623711/1‑150 (MQ=255)
                                                                                                                   aataatTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCa                                   <  1:1247729/150‑1 (MQ=255)
                                                                                                                   aataatTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTCGTGATCTACACTTATACTCTGTTGCATTATTCGCCTGAAAACACAAAATTCAGGCGTATTTTATCTATCTTTAACAAAAAATATCAACTTTCTCGAttttaactca                                   >  2:1623709/1‑143 (MQ=255)
                                                                                                                    ataatTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAg                                  <  1:859909/150‑1 (MQ=255)
                                                                                                                     taatTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGATATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGc                                 >  2:54931/1‑150 (MQ=255)
                                                                                                                           ccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTtat                           >  2:847286/1‑150 (MQ=255)
                                                                                                                           ccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTATCATCCCTtat                           >  1:1450134/1‑150 (MQ=255)
                                                                                                                           ccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTATGACAAAAAATATCAACTTTCTAGATTTGATCTCAGACCTtat                           >  1:1528933/1‑150 (MQ=255)
                                                                                                                           ccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTAAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAAATTTCTCGATTTGCTCTCAGCCATtat                           >  1:1537016/1‑150 (MQ=255)
                                                                                                                            cGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGCTACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTAGATTTGCTCTCAGCCCTtata                          >  2:1340920/1‑150 (MQ=255)
                                                                                                                              ccAAATAGTTTTTTATCAGGTATATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATc                        <  2:489385/150‑1 (MQ=255)
                                                                                                                                aaaTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCAc                      >  1:1196715/1‑150 (MQ=255)
                                                                                                                                 aaTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACg                     >  1:632619/1‑150 (MQ=255)
                                                                                                                                     gCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGaa                 <  1:1139823/150‑1 (MQ=255)
                                                                                                                                       tttttATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACAACAATATTCAGGCGTTTTTTCGATATCTTTGACAAAAAATATCAACTTTCTCGAATTGCTCTAAGACATTATATCACGGGAAAt               >  2:1287418/1‑150 (MQ=255)
                                                                                                                                            aTCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCgg          <  1:2043712/150‑1 (MQ=255)
                                                                                                                                            aTCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCgg          <  1:2052046/150‑1 (MQ=255)
                                                                                                                                             tCACGCATATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGc         <  1:2197270/150‑1 (MQ=255)
                                                                                                                                              cACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCg        >  2:837412/1‑150 (MQ=255)
                                                                                                                                              cACGCAAATAATTTGTGGTGATATACACTGATACTATGTTGAATTATTCGACTGAAACCACAATATTCAGGCGTAATTTCGCTATCTATAACAAAAAAAATAAACATTCTCGATTTGCAATAATCAATAATATAACTGGAAATTcctgag        >  2:1696210/1‑146 (MQ=255)
                                                                                                                                               aCGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGa       >  1:1473296/1‑150 (MQ=255)
                                                                                                                                                cGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGACCTTATATCACGGGAAATTCCGGCGAt      >  2:674311/1‑150 (MQ=255)
                                                                                                                                                 gCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGAtt     <  2:485531/150‑1 (MQ=255)
                                                                                                                                                     aataatTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCACTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGc  >  2:1762816/1‑150 (MQ=255)
                                                                                                                                                     aataatTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCACTCAGCCATTATATCACGGGAAATTCCGGCGATTTGc  >  2:1771654/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGC  >  NC_000913/2804386‑2804683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: