Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,838,222 A→T 8.0% intergenic (‑117/+32) hydN ← / ← ascG formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,838,2220AT 8.0% 394.2 / 17.0 135intergenic (‑117/+32)hydN/ascGformate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base A (57/67);  new base T (8/3);  total (65/70)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 140 total aligned reads displayed.

TTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATC  >  NC_000913/2838074‑2838371
                                                                                                                                                    |                                                                                                                                                     
ttACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACCAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGac                                                                                                                                                      <  2:230486/150‑1 (MQ=255)
  aCTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGacac                                                                                                                                                    >  1:195139/1‑150 (MQ=255)
   cTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACt                                                                                                                                                   >  1:1983114/1‑150 (MQ=255)
           aGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGa                                                                                                                                           >  2:2125131/1‑150 (MQ=255)
            gCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAt                                                                                                                                          >  1:1054165/1‑150 (MQ=255)
            gCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAt                                                                                                                                          >  2:1961802/1‑150 (MQ=255)
             cAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATg                                                                                                                                         >  2:100609/1‑150 (MQ=255)
             atttgatgaACCGTTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTAATTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATg                                                                                                                                         >  1:594257/4‑150 (MQ=255)
               atgatgAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGAc                                                                                                                                       <  1:2085900/150‑1 (MQ=255)
                 gatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAt                                                                                                                                     <  1:1863990/150‑1 (MQ=255)
                   tgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtgt                                                                                                                                   >  2:455043/1‑150 (MQ=255)
                   tgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAACGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtcg                                                                                                                                   <  1:822505/150‑3 (MQ=255)
                    gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCTTGCCAGTTTTTAAATTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtgtg                                                                                                                                  <  2:1587261/150‑1 (MQ=255)
                     aaccgtttCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtga                                                                                                                                 >  1:1401263/7‑150 (MQ=255)
                      aaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgat                                                                                                                                >  2:1475066/1‑150 (MQ=255)
                       aCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCAAGCCAGTTTTTAATTTACTGTTAATTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgatg                                                                                                                               <  1:280209/150‑1 (MQ=255)
                        cGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgatga                                                                                                                              >  2:1028147/1‑150 (MQ=255)
                         gtttCATCATATTCTCCATCAATGAACGTTAATGCGCCGATAGAATCACAAATCATGACAGTTTTTAATTTACTGTTATTTATGGAAATTAATTTGTGTTATGCAGGAAAAACGTTGTCATCGACACTAGTGTCGATGACATGTGATGAc                                                                                                                             <  2:1757849/148‑1 (MQ=255)
                             catcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATg                                                                                                                         >  2:1440833/1‑150 (MQ=255)
                             catcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATg                                                                                                                         <  1:997946/150‑1 (MQ=255)
                             catcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATg                                                                                                                         <  1:570789/150‑1 (MQ=255)
                              atcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGt                                                                                                                        >  1:49930/1‑150 (MQ=255)
                              atcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGt                                                                                                                        <  2:1515693/150‑1 (MQ=255)
                               tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                       >  1:159617/1‑150 (MQ=255)
                               tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                       <  2:790964/150‑1 (MQ=255)
                               tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAAGACATGTGATGACAATGtt                                                                                                                       >  1:395918/1‑150 (MQ=255)
                                caAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGttt                                                                                                                      <  2:1676391/150‑1 (MQ=255)
                                  aaTTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTAt                                                                                                                    <  1:589334/150‑1 (MQ=255)
                                    ttCTCCAGCAATGACAGTTAATTCTCCGATACAATCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAAGTAAATTCTGTAATCCAGGAAACACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcg                                                                                                                  <  2:363295/150‑1 (MQ=255)
                                     tctcCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcgc                                                                                                                 <  2:825620/150‑1 (MQ=255)
                                     tctcCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcgc                                                                                                                 <  1:16467/150‑1 (MQ=255)
                                       tcCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATTACAATGTTTATCGCGa                                                                                                               >  1:143317/1‑150 (MQ=255)
                                        ccAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGaa                                                                                                              >  1:1910855/1‑150 (MQ=255)
                                         cAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGGTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAg                                                                                                             <  1:1965666/150‑1 (MQ=255)
                                          aGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTTTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACTTGTGATGACAATGTTTATCTCTAAgg                                                                                                            >  1:895905/1‑150 (MQ=255)
                                          aGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAgg                                                                                                            <  2:2143699/150‑1 (MQ=255)
                                            cAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAg                                                                                                          <  1:842397/150‑1 (MQ=255)
                                            cAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAg                                                                                                          >  1:734895/1‑150 (MQ=255)
                                            cAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAAATAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAg                                                                                                          <  1:1002895/150‑1 (MQ=255)
                                             aaTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGc                                                                                                         >  2:1490739/1‑150 (MQ=255)
                                                 aCAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTATAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCTGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtg                                                                                                     >  1:126836/1‑150 (MQ=255)
                                                     ttAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagtg                                                                                                 <  1:859551/150‑1 (MQ=255)
                                                     ttAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagtg                                                                                                 <  1:930270/150‑1 (MQ=255)
                                                       aaTGCGCCGATACATTCTCAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACTATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagtgag                                                                                               <  1:1949139/150‑1 (MQ=255)
                                                            gcCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGATCAATGAGTGAGTcgcg                                                                                          >  2:1541390/1‑150 (MQ=255)
                                                             ccGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCgcgg                                                                                         >  2:157166/1‑150 (MQ=255)
                                                              cGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCgcggc                                                                                        <  1:1567423/150‑1 (MQ=255)
                                                                    aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCa                                                                                  <  1:769899/150‑1 (MQ=255)
                                                                      tCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGt                                                                                >  2:1170352/1‑150 (MQ=255)
                                                                       cacaAATCATGCCAGTTTTTAATTTACTTTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtt                                                                               >  2:1840388/1‑150 (MQ=255)
                                                                       cacaAATCATGCCAGATTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtt                                                                               <  2:875349/150‑1 (MQ=255)
                                                                          aaaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTc                                                                            <  2:195139/150‑1 (MQ=255)
                                                                           aaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTcc                                                                           <  2:2214141/150‑1 (MQ=255)
                                                                              caagCCAGTTTTTAAATTACTGATATTAAAGGAAATTACTTTCTGTAATGCAGGAAAAACGATGTCCTCGACACTAGGGACGACGACATGTGATGCCAAAGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCt                                                                        <  2:33792/147‑1 (MQ=255)
                                                                                agCCAGTATTTAATTTACTGTTATATAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATAGACAATAGATACTATGACATGTGATGAAAATGTTTATCGCGAAGGAGCAAAGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGa                                                                      >  1:245985/2‑150 (MQ=255)
                                                                                 gCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGaa                                                                     >  2:443595/1‑150 (MQ=255)
                                                                                  ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGAAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAg                                                                    >  1:147605/1‑150 (MQ=255)
                                                                                   cAGTTTTTAATATACTGTTCTTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAgg                                                                   <  2:653220/150‑1 (MQ=255)
                                                                                      tttttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGttt                                                                <  1:1236162/150‑1 (MQ=255)
                                                                                         ttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcg                                                             >  2:1705162/1‑150 (MQ=255)
                                                                                          tAATTTACTGTTATTTAAGGAAATTGATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcgg                                                            <  2:799353/150‑1 (MQ=255)
                                                                                            aTTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTCCGCTGAAGGTTTTcggcgg                                                         >  2:1743703/1‑150 (MQ=255)
                                                                                              ttACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCCTCGACACTAGTGACGATGACATGTGATGACAATGGTTCTCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGt                                                        <  1:967682/150‑1 (MQ=255)
                                                                                              ttACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGt                                                        <  1:565017/150‑1 (MQ=255)
                                                                                               tACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGATACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTg                                                       >  2:1349022/1‑150 (MQ=255)
                                                                                               tACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTg                                                       <  2:1054165/150‑1 (MQ=255)
                                                                                               tACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTg                                                       <  2:1846374/150‑1 (MQ=255)
                                                                                               tACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTg                                                       <  2:719076/150‑1 (MQ=255)
                                                                                               tACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTg                                                       <  1:1563029/150‑1 (MQ=255)
                                                                                                aCTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTga                                                      <  1:1924453/150‑1 (MQ=255)
                                                                                                 cTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTgag                                                     <  1:776027/150‑1 (MQ=255)
                                                                                                   gTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGaa                                                   >  1:487402/1‑150 (MQ=255)
                                                                                                      aTTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATc                                                >  2:2021559/1‑150 (MQ=255)
                                                                                                         tAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATcccc                                             >  1:1517671/1‑150 (MQ=255)
                                                                                                          aaGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCg                                            <  2:1710767/150‑1 (MQ=255)
                                                                                                          aaGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGGCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCg                                            <  2:1989274/150‑1 (MQ=255)
                                                                                                                  taattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAATTCCCAGCCATCGAg                                    >  2:258884/1‑150 (MQ=255)
                                                                                                                   aattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGGGTCGATGACAGGTGATGCCAATGTTAATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGc                                   <  1:92193/150‑1 (MQ=255)
                                                                                                                   aattTCTGTAATGCAGGAAAAACGATGGCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGc                                   <  1:2083281/150‑1 (MQ=255)
                                                                                                                    attTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCa                                  <  1:2156024/150‑1 (MQ=255)
                                                                                                                      ttCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATa                                <  1:889681/150‑1 (MQ=255)
                                                                                                                       tCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaa                               <  1:532708/150‑1 (MQ=255)
                                                                                                                          gTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaaaaa                            >  2:610948/1‑150 (MQ=255)
                                                                                                                           tAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTATATCTCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTGCGCTGAAGGTTTTCGGCGGTGAGAACTGCCCGCCATCGAGCATAAAAAt                           >  1:104622/1‑150 (MQ=255)
                                                                                                                            aaTGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATc                          >  1:1745307/1‑150 (MQ=255)
                                                                                                                             aTGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCa                         >  2:1066764/1‑150 (MQ=255)
                                                                                                                                 aGGAAAAACGATGTCATCGACACTAGTGACGAAGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCg                     <  2:395918/150‑1 (MQ=255)
                                                                                                                                  ggAAAAACGATGTCATCGACACTTGTGGCGTTGACATGTTTGGTGTATGTTTATAGGGAATGAGCTATGTGTGAGTGGCGGCGGATGAGGTTTCAGCGGAAGGTTTTAGTCGGTGCGAAGTGCACGCCGTCGAGCCTTAAAAtgatacgt                    >  2:200479/1‑143 (MQ=255)
                                                                                                                                      aaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaa                >  2:792850/1‑150 (MQ=255)
                                                                                                                                          caTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaatact            >  1:79053/2‑148 (MQ=255)
                                                                                                                                          caTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaataat            >  1:740353/2‑150 (MQ=255)
                                                                                                                                          caTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaataat            >  2:731596/2‑150 (MQ=255)
                                                                                                                                           aTGTCATCGTCACTAGTGTCGATGACATTTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGGCTCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGTTGAGAAATCCCCGCCATCGAGCATATAAATCAGCCGTCCAAAAAtt           >  2:1053868/1‑150 (MQ=255)
                                                                                                                                            tGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGATTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAAttt          >  2:1750982/1‑150 (MQ=255)
                                                                                                                                             gTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTc         >  1:593748/1‑150 (MQ=255)
                                                                                                                                              tCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTcc        <  1:2138074/150‑1 (MQ=255)
                                                                                                                                               cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt       >  1:96726/1‑150 (MQ=255)
                                                                                                                                               cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt       <  1:2004899/150‑1 (MQ=255)
                                                                                                                                                    acacTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGATCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAatc  <  1:1440833/150‑1 (MQ=255)
                                                                                                                                                    acacTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAatc  <  2:581605/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATC  >  NC_000913/2838074‑2838371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: